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http://dx.doi.org/10.5333/KGFS.2005.25.4.281

Production of Transgenic Birdsfoot trefoil Plants by Introduction of 'SWPA2 Promoter + AtNDPK2 Gene'  

Kim Ki-Yong (National Livestock Research Institute)
Jang Yo-Soon (Korea Ocean Research & Development Institute)
Kim Meing Jooung (National Livestock Research Institute)
Lim Keun Bal (National Livestock Research Institute)
Kim Won Ho (National Livestock Research Institute)
Seo Sung (National Livestock Research Institute)
Lee Sang Jin (National Livestock Research Institute)
Kwak Sang-Soo (Korea Research Institute of Bioscience & Biotechnology(KRIBB))
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.25, no.4, 2005 , pp. 281-286 More about this Journal
Abstract
To develop transgenic birdsfoot trefoil (Lotus corniculatus L.) plants tolerant to environmental stress, Arabidopsis NDPK gene (AtNDPK) was introduced into birdsfoot trefoil plants using Agrobacterium-mediated transformation and expressed powerfully under the control of the SWPA2 promoter. The expression vector, pCAMBIA2300 was used for introduction of AtNDPK gene into birdsfoot trefoil plants. The transformed calli were selected on kanamycin containing medium and then regenerated. The transformed birdsfoot trefoil plants were cultivated fur 4 months on BOi2Y medium. Genomic DNA PCR and Southern blot analysis confirmed the incorporation of AtNDPK into the birdsfoot trefoil genome.
Keywords
AtNDPK gene; Birdsfoot trefoil; Environmental stress; SWPA2 promoter; Transgenic plant;
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