• Title/Summary/Keyword: transformation and development

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A Numerical Study on the Agglomeration of Algae by the Ultrasonic Wave (초음파를 이용한 미세조류 응집에 관한 수치해석 연구)

  • Ha, Ji Soo;Shim, Sung Hun;Jung, Sang Hyun
    • Journal of Energy Engineering
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    • v.25 no.1
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    • pp.23-28
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    • 2016
  • In spite of various merit of algae as biofuel, the production cost of algae is a considerable obstacle for commercialization. The concurrent development of essential technologies is needed for the cultivating, harvesting, extracting and energy transformation. The production cost of algae biofuel has still higher than that of the other commercial biofuel. The major research activity has been focused on the cultivating and the research of other processes has been done with relatively lower activity. It is difficult to separate the algae from water because of the similar magnitude of density each other. The agglomeration and extracting of algae with the hybrid technology using ultrasonic wave is rare effect of environmental hazard and also it is appropriate technology for the next generation energy resources. The present research is investigated for the effective separation of algae from water with the ultrasonics wave. The aim of the present research is focused on the establishment of optimal design of algae agglomeration system. For this purpose, the computational fluid dynamic analysis has been conducted in the flow field with ultrasonic wave and algae flow to clarify the mechanism of algae separation by ultrasonic wave.

Construction of FMDV VP1 Gene Using Artificial DNA Synthesis and Transformation of Nicotiana tabacum Using Agrobacterium Vector System (유전자 인공합성을 이용한 구제역 유전자 VP1의 제작과 Agrobacterium Vector System을 이용한 담배 형질전환)

  • Lee, Eun-Jung;Lim, Hee-Young;Kim, Sung-Hoon;Kang, Kyung-Sun;Park, Young-Doo;Yun, Choong-Hyo;Yoon, Byoung-Su
    • Journal of Plant Biotechnology
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    • v.31 no.4
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    • pp.285-293
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    • 2004
  • FMDV is a viral pathogen that caused foot-and-mouth disease in animals. VP1 is a major capsid protein of FMDV. It is known as one of best materials for the FMDV diagnosis and for the development of protein vaccine. In this study, 633 bp of VP1 gene was modified for the expression of VP1 in plant, based on the VP1 DNA sequence from FMDV taiwan O type and from FMDV isolated vietnam. The. deduced DNA fragment was artificially synthesized using the multiple fragment extension with long-nucleotides. A new plant transgenic vector system, pCAMBIA139011 was constructed on the basis of pBI12l and pCAMBIA1390. Using this vector system and GFP gene or modified VP1 gene, each target gene was introduced into Nicotiana tabacum. The insertion of whole target gene was successfully confirmed in each transgenic plant named GFP-A7 and VP1-4, respectively. The expression level of each gene was estimated by RT-PCR and Real-Time PCR using VP1, GFP specific primers.

Recent Studies on the Edible Plant Vaccine for Prophylactic Medicine against Microorganism-Mediated Diseases (세균성 질병 예방을 위한 식물 경구 백신 연구 동향)

  • Hahn Bum-Soo;Jeong Young-Jae;Roh Kyung-Hee;Park Jong-Sug;Cho Kang-Jin;Kim Yong-Hwan;Kim Jong-Bum
    • Journal of Plant Biotechnology
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    • v.32 no.4
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    • pp.233-241
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    • 2005
  • Plants have considerable advantages for the production of antigenic proteins because they provide an inexpensive source of protein and an easy administration of vaccine. Since a publication describing edible plant vaccine of HBsAg in 1992, a number of laboratories around the world have studied the use of plants as the bioreactor to produce antigenic proteins of human or animal pathogens. Over the last ten years, these works have been mainly focused on three major strategies for the production of antigenic proteins in plants: stable genetic transformation of either the nuclear or plastid genome, or transient expression in plants using viral vectors. As many antigenic proteins have been expressed in tobacco, also several laboratories have succeeded to express genes encoding antigenic proteins in other crop plants: potato, tomato, maize, carrot, soybean and spinach. At present many works for the production of edible plant vaccine against bacteria-mediated diseases have mostly performed the studies of enterotoxins and adhesion proteins. Also the development of new-type antigens (pili, flagella, surface protein, other enterotoxin and exotoxin etc.) is required for various targets and more efficacy to immunize against microorganism pathogens. Many works mostly studied in experimental animals had good results, and phase I clinical trial of LTB clearly indicated its immunogenic ability. On the other hand, edible plant vaccines have still problems remained to be solved. In addition to the accumulation of sufficient antigen in plants, human health, environment and agriculture regulation should be proven. Also oral tolerance, the physiological response to food antigens and commensal flora is the induction of a state of specific immunological unresponsiveness, needs to be addressed before plant-derived vaccine becomes a therapeutic option.

The Study on Spatial Classification of Riverine Environment using UAV Hyperspectral Image (UAV를 활용한 초분광 영상의 하천공간특성 분류 연구)

  • Kim, Young-Joo;Han, Hyeong-Jun;Kang, Joon-Gu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.10
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    • pp.633-639
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    • 2018
  • High-resolution images using remote sensing (RS) is importance to secure for spatial classification depending on the characteristics of the complex and various factors that make up the river environment. The purpose of this study is to evaluate the accuracy of the classification results and to suggest the possibility of applying the high resolution hyperspectral images obtained by using the drone to perform spatial classification. Hyperspectral images obtained from study area were reduced the dimensionality with PCA and MNF transformation to remove effects of noise. Spatial classification was performed by supervised classifications such as MLC(Maximum Likelihood Classification), SVM(Support Vector Machine) and SAM(Spectral Angle Mapping). In overall, the highest classification accuracy was showed when the MLC supervised classification was used by MNF transformed image. However, it was confirmed that the misclassification was mainly found in the boundary of some classes including water body and the shadowing area. The results of this study can be used as basic data for remote sensing using drone and hyperspectral sensor, and it is expected that it can be applied to a wider range of river environments through the development of additional algorithms.

Development of 3-D Stereotactic Localization System and Radiation Measurement for Stereotactic Radiosurgery (방사선수술을 위한 3차원 정위 시스템 및 방사선량 측정 시스템 개발)

  • Suh, Tae-Suk;Suh, Doug-Young;Park, Sung-Hun;Jang, Hong-Seok;Choe, Bo-Young;Yoon, Sei-Chul;Shinn, Kyung-Sub;Bahk, Yong-Whee;Kim, Il-Hwan;Kang, Wee-Sang;Ha, Sung-Whan;Park, Charn-Il
    • Journal of Radiation Protection and Research
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    • v.20 no.1
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    • pp.25-36
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    • 1995
  • The purpose of this research is to develop stereotactic localization and radiation measurement system for the efficient and precise radiosurgery. The algorithm to obtain a 3-D stereotactic coordinates of the target has been developed using a Fisher CT or angio localization. The procedure of stereotactic localization was programmed with PC computer, and consists of three steps: (1) transferring patient images into PC; (2) marking the position of target and reference points of the localizer from the patient image; (3) computing the stereotactic 3-D coordinates of target associated with position information of localizer. Coordinate transformation was quickly done on a real time base. The difference of coordinates computed from between Angio and CT localization method was within 2 mm, which could be generally accepted for the reliability of the localization system developed. We measured dose distribution in small fields of NEC 6 MVX linear accelerator using various detector; ion chamber, film, diode. Specific quantities measured include output factor, percent depth dose (PDD), tissue maximum ratio (TMR), off-axis ratio (OAR). There was small variation of measured data according to the different kinds of detectors used. The overall trends of measured beam data were similar enough to rely on our measurement. The measurement was performed with the use of hand-made spherical water phantom and film for standard arc set-up. We obtained the dose distribution as we expected. In conclusion, PC-based 3-D stereotactic localization system was developed to determine the stereotactic coordinate of the target. A convenient technique for the small field measurement was demonstrated. Those methods will be much helpful for the stereotactic radiosurgery.

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Embryo-Fetal Developmental Toxicity Study of Methoxycinnamidopropyl Polysilsesquioxane (Methoxycinnamidopropyl Polysilsesquioxane의 랫드를 이용한 배.태자 발생독성 연구)

  • Hong, Jeong-Sup;Lim, Jeong-Hyeon;Kim, Kang-Hyun;Park, Myeong-Kyu;Jo, Ki-Yeon;Park, Gil-Jong;Jung, Taek-Kyu;Kim, Ja-Young;Yoon, Kyung-Sup
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.37 no.3
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    • pp.247-256
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    • 2011
  • Existing organic UV protection materials seem to be problematic due to their penetration and irritation to skin. Inorganic UV protection materials are also at issue for safety of their nano-type transformation. Therefore, the recent studies of UV protection materials have been focused not only on the effectiveness but also on their safety. One of the UV protection materials in study which have higher safety is the organic-inorganic conjugation type UV protection material. Previously, we have reported the manufacturing process, physical property and UV protection efficiency of methoxychinnamidoprophy poloysilsesquixan as a new cross-linked polymer type UV protection material. In this study, we have evaluated the effect of the methoxychinnamidoprophy poloysilsesquixan on embryo-fetal development in SD rats. This study is expected to show some definite information related to the effect on pregnancy or embryo-fetal abnormality in case of the clinical exposure of the methoxychinnamidoprophy poloysilsesquixan.

Evaluation of the Effectiveness of Surveillance on Improving the Detection of Healthcare Associated Infections (의료관련감염에서 감시 개선을 위한 평가)

  • Park, Chang-Eun
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.1
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    • pp.15-25
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    • 2019
  • The development of reliable and objective definitions as well as automated processes for the detection of health care-associated infections (HAIs) is crucial; however, transformation to an automated surveillance system remains a challenge. Early outbreak identification usually requires clinicians who can recognize abnormal events as well as ongoing disease surveillance to determine the baseline rate of cases. The system screens the laboratory information system (LIS) data daily to detect candidates for health care-associated bloodstream infection (HABSI) according to well-defined detection rules. The system detects and reserves professional autonomy by requiring further confirmation. In addition, web-based HABSI surveillance and classification systems use discrete data elements obtained from the LIS, and the LIS-provided data correlates strongly with the conventional infection-control personnel surveillance system. The system was timely, acceptable, useful, and sensitive according to the prevention guidelines. The surveillance system is useful because it can help health care professionals better understand when and where the transmission of a wide range of potential pathogens may be occurring in a hospital. A national plan is needed to strengthen the main structures in HAI prevention, Healthcare Associated Prevention and Control Committee (HAIPCC), sterilization service (SS), microbiology laboratories, and hand hygiene resources, considering their impact on HAI prevention.

Entomopathogenic Fungi-mediated Pest Management and R&D Strategy (곤충병원성 진균을 활용한 해충 관리와 개발 전략)

  • Lee, Se Jin;Shin, Tae Young;Kim, Jong-Cheol;Kim, Jae Su
    • Korean journal of applied entomology
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    • v.61 no.1
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    • pp.197-210
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    • 2022
  • Entomopathogenic fungi can be used to control a variety of sucking and chewing insects, with little effect on beneficial insects and natural enemies. Approximately 170 entomopathogenic fungal insecticides have been registered and used worldwide, with the recent focus being on the mode of action and mechanism of insect-fungal interactions. During the initial period of research and development, the industrialization of entomopathogenic fungi focused on the selection of strains with high virulence. However, improvement in productivity, including securing resistance to environmental stressors, is a major issue that needs to be solved. Although conidia are the primary application propagules, efforts are being made to overcome the limitations of blastospores to improve the economic feasibility of the production procedure. Fungal transformation is also being conducted to enhance insecticidal activity, and molecular biology is being used to investigate functions of various genes. In the fungi-based pest management market, global companies are setting up cooperative platforms with specialized biological companies in the form of M&As or partnerships with the aim of implementing a tank-mix strategy by combining chemical pesticides and entomopathogenic fungi. In this regard, understanding insect ecology in the field helps in providing more effective fungal applications in pest management, which can be used complementary to chemicals. In the future, when fungal applications are combined with digital farming technology, above-ground applications to control leaf-dwelling pests will be more effective. Therefore, for practical industrialization, it is necessary to secure clear research data on intellectual property rights.

Crisis of trust of journalism in France: Cracks in journalistic institutions and professionalism, and the impact of social movement (프랑스 언론의 신뢰도 위기: 저널리즘 제도의 내적 균열과 사회운동의 영향)

  • Park, Jin woo;Kim, Soel ah
    • Analyses & Alternatives
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    • v.6 no.2
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    • pp.185-226
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    • 2022
  • This study examines the crisis of trust of French journalism in the context of a global decline of media credibility. First of all, in the process of a huge social movement called the 'yellow vest' movement that started in 2018, distrust of the French journalism was expressed in an extreme form. This study examines some external factors in terms of the historical development of the French journalism and the public's long-standing 'criticism of journalism'. Specifically, this study first examines the quantitative indicators of trust of French journalism which were shown in Digital News Report published by the Reuters Institute for the Study of Journalism. Next, it examines the historical and institutional formation process of French journalism and public distrust that emerged along with it. And specifically, the structural crisis-economic crisis, digital transformation and intensification of competition, and deterioration of quality problems etc.-of the French journalism exposed in media coverage on social movement in 2018 is review in relation with the working process or 'routine' of actual news production. In conclusion, this study asserts that the various aspects of internal rifts in French journalism system, as well as external shocks (the influence of social movements), are a key factor in explaining the recent decline of trust in French journalism.

Development and Application of Cellulose Nanofiber Powder as a Nucleating Agent in Polylactic Acid (나노셀룰로오스 분말 개발과 폴리젖산 내 핵제 적용 연구)

  • Sanghyeon Ju;Ajeong Lee;Youngeun Shin;Teahoon Park
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.29 no.1
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    • pp.51-57
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    • 2023
  • Because of the global pollution caused by plastic disposal, demand for eco-friendly transformation in the packaging industry is increased. As part of that, the utilization of polylactic acid (PLA) as a food packaging material is increased. However, it is necessary to improve the crystallinity of PLA by adding nucleating agents or to improve the modulus by adding fillers because of the excessive brittleness of the PLA matrix. Thus, the cellulose nanofiber (CNF) was fabricated and dried to obtain a powder form and applied to the CNF/PLA nanocomposite. The effect of CNF on the morphological, thermal, rheological, and dynamic mechanical properties of the composite was analyzed. We can confirm the impregnated CNF particle in the PLA matrix through the field emission scanning electron microscope (FE-SEM). Differential scanning calorimetry (DSC) analysis showed that the crystallinity of not annealed CNF/PLA nanocomposite was increased approximately 2 and 4 times in the 1st and 2nd cycle, respectively, with the shift to lower temperature of cold crystallization temperature (Tcc) in the 2nd cycle. Moreover, the crystallinity of annealed CNF/PLA nanocomposite increased by 13.4%, and shifted Tcc was confirmed.