• The Plant Pathology Journal
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• v.18 no.4
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• pp.187-191
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• 2002

This paper describes the cloning and sequence analysis of the 5'-terminal region and full-length cDNA production of genomic RNA of Lily symptomless virus (LSV), a Species Of the genus Carlavirus. A sing1e DNA band about 600 bp harboring the 5'-end of genomic RNA of the virus was successfully amplified by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE), and was cloned for nucleotide sequence determination. Sequence analysis of selected RACE cDNA clones revealed that the LSV 5'non-translated region consists of 67 nucleotides long of AT rich stretch followed GC rich from the 5'-end. To produce full-length cDNA products for the viral genomic RNA, a set of LSV-specific primers could be designed based on the obtained sequence in this study and the known sequences of 3'-terminal region for the virus. Full-length cDNA copies of LSV, an 8.4 kb long, were directly amplified by the long-template RT-PCR technique from the purified viral genomic RNA samples. This full-length cDNA copies were analyzed by restriction mapping. The molecules produced in this study can be useful for the production of in vitro infectious cDNA clone, as well as, for the completion of genomic RNA sequence and genome structure for the virus.

• MALSORI
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• no.37
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• pp.49-71
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• 1999

The Purpose of this paper is to gather pairs of heteronyms in modern Korean and transcribe them in the IPA and the Roman alphabet in order to propose that all of them should be differentiated in Hanngul orthography. More than a quarter of the whole Korean vocabulary consists of words with a long vowel and the number of minimal pairs distinguished only by the chroneme reaches nearly ten thousand (i.e. twenty thousand words). The letter h syllable-finally is used here to represent the long vowel in Romanization except the vowel '으‘[?:] which is transcribed by doubling the letter u (i.e. uu). Another factor bringing forth lots of heteronyms in Korean is the lack of full indication as to the non-automatic reinforcement in the initial consonant of a word (or a morpheme) when preceded by another within a phrase (or a word). These reinforced word-initial consonants are written with the letter c and an apostrophe (like c'g- , c'd- , c'b-, c's-, c'j-) in Romanization here. The reinforced morpheme-initial consonant within a word is written with the letters k t, p, ss and cz for ㄲ, ㄸ, ㅃ, ㅆ and ㅉ sounds respectively. The contrasted pronunciations of pairs of heteronyms beginning with ㅅ /s/sup h// and ㅆ /s/ sounds are transcribed here for exemplification.

• MALSORI
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• no.50
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• pp.51-69
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• 2004

In this paper, we present a one-pass semi-dynamic network decoding framework that inherits both advantages of fast decoding speed from static network decoders and memory efficiency from dynamic network decoders. Our method is based on the novel language model network representation that is essentially of finite state machine (FSM). The static network derived from the language model network [1][2] is partitioned into smaller subnetworks which are static by nature or self-structured. The whole network is dynamically managed so that those subnetworks required for decoding are cached in memory. The network is near-minimized by applying the tail-sharing algorithm. Our decoder is evaluated on the 25k-word Korean broadcast news transcription task. In case of the search network itself, the network is reduced by 73.4% from the tail-sharing algorithm. Compared with the equivalent static network decoder, the semi-dynamic network decoder has increased at most 6% in decoding time while it can be flexibly adapted to the various memory configurations, giving the minimal usage of 37.6% of the complete network size.

• BMB Reports
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• v.37 no.3
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• pp.320-334
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• 2004

StMBF1 (Solanum tuberosum multiprotein bridging factor 1) is a plant member of the MBF1 family of transcriptional co-activators. In an attempt to understand the role of StMBF1, we analyzed its interaction with plant transcription factors of the homeodomain-leucine zipper (Hd-Zip) family, a group of proteins with a typical leucine zipper motif adjacent to a homeodomain. StMBF1 is able to interact in vitro with the Hd-Zip protein Hahb-4 both in the presence and absence of DNA. Upon binding, StMBF1 increases the DNA binding affinity of Hahb-4, and of another plant homeodomain containing protein from the GL2/Hd-Zip IV family, HAHR-1. The biological role of interactions is discussed in this paper.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.1-6
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• 2001

This paper reports a few characteristics of seven insect mitochondrial genomes sequenced completely (Bombyx mori, Drosophila melanogaster, D. yakuba, Apis mellifera, Anopheles gambiae, A. quadrimaculatus, and Locusta migratoria). Comparative analysis of complete mt genome sequences from several species revealed a number of interesting features (base composition, gene content, A+T-rich region, and gene arrangement, etc) of insect mitochondrial genome. The properties revealed by our work shed new light on the organization and evolution of the insect mitochondrial genome and more importantly open up the way to clearly aimed experimental studies for understanding critical roles of the regulatory mechanisms (transcription and translation) in mitochondrial gene expression.

• The KIPS Transactions:PartB
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• v.11B no.1
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• pp.99-106
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• 2004

The syllable restoration is a process restoring a phonetic value recognized in a speech recognition device with the notation form that a vocalization is former. In this paper a syllable restoration rule was composed of a based on standard pronunciation for a syllable restoration process. A syllable restoring regulation was used, and a generation method of a notation candidate set was researched. Also, A study is held to reduce the number of created notation candidate. Three phases of reduction processes were suggested. Reduction of a notation candidate has the non-notation syllable, non-vocabulary syllable and non-stem syllable. As a result of experiment, an average of 74％ notation candidate decrease rates were shown.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.1101-1107
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• 2015

The role of CRE1 in a thermophilic fungus, Myceliophthora thermophila ATCC42464, was studied using RNA interference. In the cre1-silenced strain C88, the filter paper hydrolyzing activity and β-1,4-endoglucanase activity were 3.76-, and 1.31-fold higher, respectively, than those in the parental strain when the strains were cultured in inducing medium for 6 days. The activities of β-1,4-exoglucanase and cellobiase were 2.64-, and 5.59-fold higher, respectively, than those in the parental strain when the strains were cultured for 5 days. Quantitative reverse-transcription polymerase chain reaction showed that the gene expression of egl3, cbh1, and cbh2 was significantly increased in transformant C88 compared with the wild-type strain. Therefore, our findings suggest the feasibility of improving cellulase production by modifying the regulator expression, and an attractive approach to increasing the total cellulase productivity in thermophilic fungi.

• Microbiology and Biotechnology Letters
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• v.36 no.2
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• pp.149-157
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• 2008

Many proteins consist of distinctive domains that can act independently or cooperatively to achieve a unique function. As these domains evolve from a naturally existing repertoire of functional domains, this implies that domain organization is an intrinsic element involved in building the complex structure and function of proteins. Thus, identifying functional domains would appear to be critical to the elucidation of questions related to protein evolution, folding, and the engineering of hybrid proteins for tai- lored applications. However, the simple application of "Lego-like assembly" to the engineering of hybrid proteins is an oversimplification, as many hybrid constructs lack structural stability, usually due to unfavorable domain contacts. Thus, directed evolution, along with computational studies, may help to engineer hybrid proteins with improved physico-chemical properties. Accordingly, this paper introduces several approaches to functional hybrid protein engineering that potentially can be used to create modulators of gene transcription and cell signaling, and novel biosensors to analyze biological functions in vivo.

• Genomics & Informatics
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• v.5 no.1
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• pp.1-5
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• 2007

Embryonic stem cells can be differentiated into various types of cells, requiring a tight regulation of transcription. Biomarkers related to each lineage of cells are used to guide the differentiation into neural or any other fates. In previous experiments, we reported the guided differentiation (GD)-specific genes by comparing profiles of random differentiation (RD). Interestingly 68% of differentially expressed genes in GD overlap with that of RD, which makes it difficult for us to separate the lineages by examining several markers. In this paper, we design a prediction model to identify the differentiation into neural fates from any other lineage. From the profiles of 11,376 genes, 203 differentially expressed genes between neural and random differentiation were selected by random variance T-test with 95% confidence and 5% false discovery rate. Based on support vector machine algorithm, we could select 79 marker genes from the 203 informative genes to construct the optimal prediction model. Here we propose a prediction model for the prediction of neural fates from random differentiation which is constructed with a perfect accuracy.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.8 s.227
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• pp.1183-1189
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• 2004

A novel cell count method, which can improve the count efficiency and reduce contamination problem, was presented using micro lattice engraved on culture dish. The micro lattice has feature of $50{\mu}m{\times}50{\mu}m$ rectangular shape, $2{\mu}m$ line width, and $2{\mu}m$ depth in $3mm{\times}3mm$ area. In this paper, nickel mold was fabricated with thickness of 3mm and diameter of 80 mm, and transcription of the micro lattice on a polystyrene cell culture dish was performed by hot embossing at $200\;^{\circ}C$. The tedious and error-prone harvest/load processes of conventional cell counts with a hemocytometer could be omitted, and these advantages became magnified during periodical counts involving long-term cultures. SupT1 cells and HeLa cells were cultivated with the dish for 7 days in $CO_2$ incubator and counted as $371.84/mm^2$ and $123.36/mm^2$, respectively, during the cultivation without harmful effects on the cells.