• The Journal of the Acoustical Society of Korea
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• v.15 no.2
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• pp.61-71
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• 1996

In this paper, dialogic triphone data base construction for triphone synthesis system is discussed. Particularly, in this work, dialogic speech data is collected from the broadcast media, and three different transcription steps are taken. Total 10 hours of speech data are collected. Among them, six hours of speech data are used for the triphone data base construction, and the rest four hours of data are reserved. Dialogic speech data base construction is far different from the reciting speech data base construction. This paper describes various steps that necessary for the dialogic triphone data base construction from collecting speech data to triphone unit labeling.

• Journal of the Korean Institute of Intelligent Systems
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• v.23 no.4
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• pp.371-377
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• 2013

This paper proposes three methods for improving the accuracy of auto-music transcription considering with time-varying tempo from monophonic sound. The first one that uses TV(Total Variation) filter for smoothing the pitch data reduces the fragmentation in the pitch segmentation result. Also, the measure finding method that combines three different ways based on pitch and energy of sound data, respectively as well as based on rules produces more stable result. In addition the temporal result of note-length encoding is corrected in optimal way that the resulted encoding minimizes the sum of quantization error in a measure while the sum of note-lengths is equal to the number of beats. In the experiment with 16 children songs, we obtained the improved result in which measure finding was complete, the accuracy of encoding for note-length and pitch was about 91.3 and 86.7, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.11
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• pp.3280-3286
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• 2009

In this paper, we designed and manufactured HD Switch Panel parts with micro-etched pattern. By using CAE analysis, we could predict weld-line positions and the amount of shrinkage during injection molding process. We measured the temperature distribution of the mold surface so that we could optimize the processing conditions such as mold temperature. In order to increase the transcription of micro-etched pattern, we applied the E-Mold technology and evaluated the effect of mold temperature on the surface quality. We concluded that the etched pattern was improved(more than 2.5$\to$1.5~1.7) through the measurement of surface gloss and the observation of both SEM and SPM images.

• The Journal of the Korea Contents Association
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• v.12 no.11
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• pp.9-19
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• 2012

Time-varying tempo of a song is one of the error sources for the identification of a note duration in automatic music recognition. This paper proposes an improved music transcription scheme equipped with the identification of note duration considering the time-varying tempo. In the proposed scheme the measures are found at first and the tempo, the playing time of each measure, is then estimated. The tempo is then used for resizing each IOI(Inter Onset Interval) length and considered to identify the accurate note duration, which increases the degree of correspondence to the music piece. In the experiment the proposed scheme found the accurate measure position for 14 monophonic children songs out of 16 ones recorded by men and women. Also, it achieved about 89.4% and 84.8% of the degree of matching to the original music piece for identification of note duration and pitch, respectively.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.928-934
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• 2005

ATF2 is a cellular transcription factor which belongs to the CREB/ATF class and it is leucine zipper protein which generally binds to DNA as dimers. This paper presents the procedure for subcloning the ATF2 gene and the results of experiment used the expressed ATF2. The pET expression vector was used since it produced 6xHis fusion protein for easy purification using affinity column. The Nickel chelating chromatography was used for Purifying the expressed ATE2 from E- codi BL2l. Subsequen시y In vitro binding pull-down assay showed the binding specificity of ATF2 with AP-1 family factors such as BATF, c-Fos, c-Jun and ATF2 itselgf. ATF2 forms homodimer as well as strong heterodimer with BATF. It also forms stable dimer with c-Jun but barely binds with c-Fos.

• Proceedings of the KSPS conference
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• 2002.11a
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• pp.81-85
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• 2002

We present a statistical analysis of Korean phonological variations using automatic generation of phonetic transcription. We have constructed the automatic generation system of Korean pronunciation variants by applying rules modeling obligatory and optional phonemic changes and allophonic changes. These rules are derived from knowledge-based morphophonological analysis and government standard pronunciation rules. This system is optimized for continuous speech recognition by generating phonetic transcriptions for training and constructing a pronunciation dictionary for recognition. In this paper, we describe Korean phonological variations by analyzing the statistics of phonemic change rule applications for the 60,000 sentences in the Samsung PBS(Phonetic Balanced Sentence) Speech DB. Our results show that the most frequently happening obligatory phonemic variations are in the order of liaison, tensification, aspirationalization, and nasalization of obstruent, and that the most frequently happening optional phonemic variations are in the order of initial consonant h-deletion, insertion of final consonant with the same place of articulation as the next consonants, and deletion of final consonant with the same place of articulation as the next consonants. These statistics can be used for improving the performance of speech recognition systems.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2005.09a
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• pp.421-426
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• 2005

In this paper, we investigate the nonlinear dynamic behavior of TPP (thiamine pyrophosphate) riboswitches in E. coli (Escherichia coli). TPP riboswitches are highly conserved RNA regulatory elements, embedded within the 5’'untranslated region of three TPP biosynthesis operons. The three operons thiCEFSGH, thiMD, and thiBPQ are involved in the biosynthesis, salvage, and transport of TPP, respectively. TPP riboswitches modulate their expressions in response to changing TPP concentration, without involving protein cofactors. Interestingly, the expression of thiMD is regulated at the translational level, while that of thiCEFSGH at both levels of transcription and translation. We develop a mathematical model of the TPP riboswitch’s regulatory system possessed by thiCEFSGH and thiMD, so as to simulate the time-course experiments of TPP biosynthesis in E. coli. The simulation results are validated against three sets of reported experimental data in order to gain insight into the nature of steady states and the stability of TPP riboswitches, and to explain the biological significance of regulating at level of transcription or translation, or even both. Our findings suggest that in the TPP biosynthesis pathway of E. coli, the biological effect of down-regulating thiCEFSGH operon at the translational level by TPP riboswitch is less prominent than that at the transcriptional level.

• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.93-103
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• 2015

The isolation, cloning, and characterization of an R2R3-MYB transcription factor gene (MtMYB70) from the model legume Medicago truncatula is reported. MtMYB70 consists of a 768-bp coding sequence corresponding to 255 amino acids. Sequence alignment revealed that MtMYB70 cDNA contains conserved R2R3-type MYB domains with highly divergent C terminal regions. MtMYB70 was found to have relatively low sequence homology with known R2R3-MYB genes. Phylogenetic analysis placed the R2R3-MYB domain of MtMYB70 closest to PtMYB1, a known activator of lignin biosynthesis. Overexpression of MtMYB70 under the control of the 35S promoter in transgenic poplar did not cause a significant difference in total lignin content relative to the control, but glucan content was significantly increased in transgenic poplar. Therefore, MtMYB70 might have regulatory role in the biosynthesis of cell wall structural carbohydrates.

• Journal of KIISE:Software and Applications
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• v.30 no.3_4
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• pp.379-387
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• 2003

A lot of microbial genome projects have been completed to pour the enormous amount of genomic sequence data. In this context. the problem of identifying promoters in genomic DNA sequences by computational methods has attracted considerable research attention in recent years. In this paper, we propose a new model of prokaryotic core promoter region including the -10 region and transcription initiation site, that is Dependency-Reflecting Decomposition Model (DRDM), which captures the most significant biological dependencies between positions (allowing for non-adjacent as well as adjacent dependencies). DRDM showed a good result of performance test and it will be employed effectively in predicting promoters in long microbial genomic Contigs.

• The KIPS Transactions:PartB
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• v.16B no.2
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• pp.141-150
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• 2009

This paper proposes a system that can automatically write singing voices to music notes. First, the system uses Stabilized Diffusion Equation(SIDE) to divide the song to a series of syllabic parts based on pitch detection. By the song segmentation, our method can recognize the sound length of each fragment through clustering based on genetic algorithm. Moreover, this study introduces a concept called 'Relative Interval' so as to recognize interval based on pitch of singer. And it also adopted measure extraction algorithm using pause data to implement the higher precision of song transcription. By the experiments using 16 nursery songs, it is shown that the measure recognition rate is 91.5% and DMOS score reaches 3.82. These findings demonstrate effectiveness of system performance.