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http://dx.doi.org/10.5010/JPB.2015.42.2.93

Modification of cell wall structural carbohydrate in the hybrid poplar expressing Medicago R2R3-MYB transcription factor MtMYB70  

Kim, Sun Hee (National Academy of Agricultural Science, Rural Development Administration)
Choi, Young Im (Division of Forest Biotechnology, Korea Forest Research Institute)
Jin, Hyunjung (Department of Biosystems Engineering, Korea University)
Shin, Soo-Jeong (Department of Wood and Paper Science, Chungbuk National University)
Park, Jong-Sug (National Academy of Agricultural Science, Rural Development Administration)
Kwon, Mi (Green Plant Institute)
Publication Information
Journal of Plant Biotechnology / v.42, no.2, 2015 , pp. 93-103 More about this Journal
Abstract
The isolation, cloning, and characterization of an R2R3-MYB transcription factor gene (MtMYB70) from the model legume Medicago truncatula is reported. MtMYB70 consists of a 768-bp coding sequence corresponding to 255 amino acids. Sequence alignment revealed that MtMYB70 cDNA contains conserved R2R3-type MYB domains with highly divergent C terminal regions. MtMYB70 was found to have relatively low sequence homology with known R2R3-MYB genes. Phylogenetic analysis placed the R2R3-MYB domain of MtMYB70 closest to PtMYB1, a known activator of lignin biosynthesis. Overexpression of MtMYB70 under the control of the 35S promoter in transgenic poplar did not cause a significant difference in total lignin content relative to the control, but glucan content was significantly increased in transgenic poplar. Therefore, MtMYB70 might have regulatory role in the biosynthesis of cell wall structural carbohydrates.
Keywords
MtMYB70; Medicago truncatula; hybrid poplar; R2R3-MYB; xylem; glucan;
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