Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Modification of cell wall structural carbohydrate in the hybrid poplar expressing Medicago R2R3-MYB transcription factor MtMYB70

  • Kim, Sun Hee (National Academy of Agricultural Science, Rural Development Administration) ;
  • Choi, Young Im (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Jin, Hyunjung (Department of Biosystems Engineering, Korea University) ;
  • Shin, Soo-Jeong (Department of Wood and Paper Science, Chungbuk National University) ;
  • Park, Jong-Sug (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kwon, Mi (Green Plant Institute)
  • Received : 2015.03.26
  • Accepted : 2015.05.08
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The isolation, cloning, and characterization of an R2R3-MYB transcription factor gene (MtMYB70) from the model legume Medicago truncatula is reported. MtMYB70 consists of a 768-bp coding sequence corresponding to 255 amino acids. Sequence alignment revealed that MtMYB70 cDNA contains conserved R2R3-type MYB domains with highly divergent C terminal regions. MtMYB70 was found to have relatively low sequence homology with known R2R3-MYB genes. Phylogenetic analysis placed the R2R3-MYB domain of MtMYB70 closest to PtMYB1, a known activator of lignin biosynthesis. Overexpression of MtMYB70 under the control of the 35S promoter in transgenic poplar did not cause a significant difference in total lignin content relative to the control, but glucan content was significantly increased in transgenic poplar. Therefore, MtMYB70 might have regulatory role in the biosynthesis of cell wall structural carbohydrates.

Keywords

References

  1. Agarwal M, Hao Y, Kapoor A, Dong CH, Fujii H, Zheng X, Zhu JK. 2006. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. J Biol. Chem. 281:37636-37645 https://doi.org/10.1074/jbc.M605895200
  2. Baumann K, Perez-Rodriguez M, Bradley D, Venail J, Bailey P, Jin H, Koes R, Roberts K, Martin C. 2007. Control of cell and petal morphogenesis by R2R3-MYB transcription factors. Development 134:1691-701 https://doi.org/10.1242/dev.02836
  3. Barker DG, Bianchi S, Blondon F, Dattee Y, Duc G, Essad S, Flament P, Gallusci P, Genier G, Guy P, Muel X, Tourneur J, Denarie J, Huguet T. 1990. Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobiumlegume symbiosis. Plant Mol. Biol. Rep. 8:40-49 https://doi.org/10.1007/BF02668879
  4. Bedon F, Grima-Pettenati J, Mackay J. 2007. Conifer R2R3-MYB transcription factors: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca). BMC Plant Biol. 7:17 https://doi.org/10.1186/1471-2229-7-17
  5. Bell CJ, Dixon RA, Farmer AD, Flores R, Inman J, Gonzales RA, Harrison MJ, Paiva NL, Scott AD, Weller JW, May GD. 2001. The Medicago genome initiative: a model legume database. Nucleic Acids Res. 29:114-117 https://doi.org/10.1093/nar/29.1.114
  6. Bhargava A, Ahad A, Wang S, Mansfield SD, Haughn GW, Douglas CJ, Ellis BE. 2013. The interacting MYB75 and KNAT7 transcription factors modulate secondary cell wall deposition both in stems and seed coat in Arabidopsis. Planta 237:1199-1211 https://doi.org/10.1007/s00425-012-1821-9
  7. Bomal C, Bedon F, Caron S, Mansfield SD, Levasseur C, Cooke JE, Blais S, Tremblay L, Morency MJ, Pavy N, Grima-Pettenati J, Seguin A, Mackay J. 2008. Involvement of Pinus taedaMYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis. J. Exp. Bot. 59:3925-3939 https://doi.org/10.1093/jxb/ern234
  8. Carletti G, Lucini L, Busconi M, Marocco A, Bernardi J. 2013. Insight into the role of anthocyanin biosynthesis-related genes in Medicago truncatula mutants impaired in pigmentation in leaves. Plant Physiol. Biochem.70:123-132 https://doi.org/10.1016/j.plaphy.2013.05.030
  9. Chai G, Wang Z, Tang X, Yu L, Qi G, Wang D, Yan X, Kong Y, Zhou G. 2014. R2R3-MYB gene pairs in Populus: evolution and contribution to secondary wall formation and flowering time. J. Exp. Bot. 65:4255-4269 https://doi.org/10.1093/jxb/eru196
  10. Choi H-K, Mun J-H, Kim D-J, Zhu H, Baek J-M, Mudge J, Roe B, Ellis N, Doyle J, Kiss GB, Young ND, Cook DR. 2004. Estimating genome conservation between crop and model legume species. Proc. Natl. Acad. Sci. 101:15289-15294 https://doi.org/10.1073/pnas.0402251101
  11. Choi YI, Noh EW, Lee HS, Han MS, Lee JS, Choi KS. 2007. Mercury-tolerant transgenic poplars expressing two bacterial mercury-metabolizing genes. J. Plant Biol. 50:658-662 https://doi.org/10.1007/BF03030610
  12. Crane C, Wright E, Dixon RA, Wang Z-Y. 2006. Transgenic Medicago truncatula plants obtained from Agrobacterium tumefaciens-transformed roots and Agrobacterium rhizogenestransformed hairy roots. Planta 223:1344-1354 https://doi.org/10.1007/s00425-006-0268-2
  13. Deluc L, Barrieu F, Marchive C, Lauvergeat V, Decendit A, Richard T, Carde JP, Merillon JM, Hamdi S. 2006. Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway. Plant Physiol.140:499-511 https://doi.org/10.1104/pp.105.067231
  14. Fornale S, Sonbol FM, Maes T, Capellades M, Puigdomenech P, Rigau J, Caparros-Ruiz D. 2006. Down-regulation of the maize and Arabidopsis thaliana caffeic acid O-methyltransferase genes by two new maize R2R3-MYB transcription factors. Plant Mol. Biol. 62:809-823 https://doi.org/10.1007/s11103-006-9058-2
  15. Glover BJ, Perez-Rodriguez M, Martin C. 1998. Development of several epidermal cell types can be specified by the same MYB-related plant transcription factor. Development 125: 3497-3508
  16. Goicoechea M, Lacombe E, Legay S, Mihaljevic S, Rech P, Jauneau A, Lapierre C, Pollet B, Verhaegen D, Chaubet-Gigot N, Grima-Pettenati J. 2005. EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesis. Plant J. 43:553-567 https://doi.org/10.1111/j.1365-313X.2005.02480.x
  17. Jung C, Seo JS, Han SW, Koo YJ, Kim CH, Song SI, Nahm BH, Choi YD, Cheong JJ. 2008. Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis. Plant Physiol.146:623-635
  18. Krishnamurthy KV. 1999. Methods in cell wall cytochemistry. USA: CRC press, Boca Raton, FL.336 pp
  19. Legay S, Sivadon P, Blervacq AS, Pavy N, Baghdady A, Tremblay L, Levasseur C, Ladouce N, Lapierre C, Seguin A, Hawkins S, Mackay J, Grima-Pettenati J. 2010. EgMYB1, an R2R3-MYB transcription factor from eucalyptus negatively regulates secondary cell wall formation in Arabidopsis and poplar. New Phytol. 188:774-778 https://doi.org/10.1111/j.1469-8137.2010.03432.x
  20. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-${\Delta}$${\Delta}$CT method. Methods 25:402-408 https://doi.org/10.1006/meth.2001.1262
  21. Lloyd G, McCown B. 1981. Commercially feasible micropropagation of Mountain Laurel, Kalmia latifolia, by use of shoot tip culture. Combined Proceedings-International Plant Propagator's Society 30:421-427
  22. Ma QH, Wang C, Zhu HH. 2011. TaMYB4 cloned from wheat regulates lignin biosynthesis through negatively controlling the transcripts of both cinnamyl alcohol dehydrogenase and cinnamoyl-CoA reductase genes. Biochimie 93:1179-1186 https://doi.org/10.1016/j.biochi.2011.04.012
  23. Meshitsuka G, Nakano K. 1979. Studies on the mechanism of lignin color reaction (XIII). Mokuzai Gakkaishi 25:588-594
  24. Moon D, Shin S-J, Choi JW, Park J-S, Kim W, Kwon M. 2011. Chemical modification of secondary xylem under tensile stress in the stem of Liriodendron tulipifera. For. Sci. Technol. 7:53-59
  25. Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  26. Nakatsuka T, Haruta KS, Pitaksutheepong C, Abe Y, Kakizaki Y, Yamamoto K, Shimada N, Yamamura S, Nishihara M. 2008. Identification and characterization of R2R3-MYB and bHLH transcription factors regulating anthocyanin biosynthesis in gentian flowers. Plant Cell. Physiol. 49:1818-1829 https://doi.org/10.1093/pcp/pcn163
  27. Newman LJ, Perazza DE, Juda L, Campbell MM. 2004. Involvement of the R2R3-MYB, AtMYB61, in the ectopic lignification and dark-photomorphogenic components of the det3 mutant phenotype. Plant J. 37:239-250 https://doi.org/10.1046/j.1365-313X.2003.01953.x
  28. Ogata K, Morikawa S, Nakamura H, Hojo H, Yoshimura S, Zhang R, Aimoto S, Ametani Y, Hirata Z, Sarai A, et al. 1995. Comparison of the free and DNA-complexed forms of the DNA-binding domain from c-myb. Nat. Struct. Biol. 2: 309-320 https://doi.org/10.1038/nsb0495-309
  29. Omer S, Kumar S, Khan BM. 2013. Over-expression of a subgroup 4 R2R3 type MYB transcription factor gene from Leucaena leucocephala reduces lignin content in transgenic tobacco. Plant Cell Rep.32:161-171 https://doi.org/10.1007/s00299-012-1350-9
  30. Park JS, Kim JB, Cho KJ, Cheon CI, Sung MK, Choung MG, Roh KH. 2008. Arabidopsis R2R3-MYB transcription factor AtMYB60 functions as a transcriptional repressor of anthocyanin biosynthesis in lettuce (Lactuca sativa). Plant Cell Rep. 27:985-994 https://doi.org/10.1007/s00299-008-0521-1
  31. Prosperi JM, Auricht G, Genier G, Johnson R. 2001. Medics (Medicago L.). Pages 99-114 in N. Maxtedand S.J. Bennet,eds. Plant Genetic Resources of Legume in the Mediterranean. Kluwer Academic Publishers, Dordrecht, Netherlands
  32. Patzlaff A, McInnis S, Courtenay A, Surman C, Newman LJ, Smith C, Bevan MW, Mansfield S, Whetten RW, Sederoff RR, Campbell MM. 2003. Characterization of a pine MYB that regulates lignification. Plant J. 36:743-754 https://doi.org/10.1046/j.1365-313X.2003.01916.x
  33. Rabinowicz PD, Braun EL, Wolfe AD, Bowen B, Grotewold E. 1999. Maize R2R3-MYB genes: Sequence analysis reveals amplification in the higher plants. Genetics 153:427-444
  34. Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G. 2000. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science 290:2105-2110 https://doi.org/10.1126/science.290.5499.2105
  35. Romano JM, Dubos C, Prouse MB, Wilkins O, Hong H, Poole M, Kang KY, Li E, Douglas CJ, Western TL, Mansfield SD, Campbell MM. 2012. AtMYB61, an R2R3-MYB transcription factor, functions as a pleiotropic regulator via a small gene network. New Phytol. 195:774-786 https://doi.org/10.1111/j.1469-8137.2012.04201.x
  36. Rubin EM. 2008. Genomics of cellulosic biofuels. Nature 454:841-844 https://doi.org/10.1038/nature07190
  37. Sambrook J, Russell DW. 2001. Molecular cloning. 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 2100 pp
  38. Shen H, He X, Poovaiah CR, Wuddineh WA, Ma J, Mann DG, Wang H, Jackson L, Tang Y, Stewart CN Jr., Chen F, Dixon RA. 2012. Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks. New Phytol. 193:121-136 https://doi.org/10.1111/j.1469-8137.2011.03922.x
  39. Stracke R, Werber M, Weisshaar B. 2001. The R2R3-MYB gene family in Arabidopsis thaliana. Curr. Opin. Plant Biol. 4:447-456 https://doi.org/10.1016/S1369-5266(00)00199-0
  40. Tian Q, Wang X, Li C, Lu W, Yang L, Jiang Y, Luo K. 2013. Functional characterization of the poplar R2R3-MYB transcription factor PtoMYB216 involved in the regulation of lignin biosynthesis during wood formation. PLoS One 8:e76369 https://doi.org/10.1371/journal.pone.0076369
  41. Wang S, Li E, Porth I, Chen JG, Mansfield SD, Douglas, C.J. 2014. Regulation of secondary cell wall biosynthesis by poplar R2R3-MYB transcription factor PtrMYB152 in Arabidopsis. Sci. Rep. 23:5054
  42. Wilkins O, Nahal H, Foong J, Provart NJ, Campbell MM. 2009. Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiol.149:981-993
  43. Young ND, Debelle F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berges H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonzalez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, Jocker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, Denarie J, Dixon RA, May GD, Schwartz DC, Rogers J, Quetier F, Town CD, Roe BA. 2011. The Medicago genome provides insight into the evolution of rhizobial symbioses. Nature 480:520-524 https://doi.org/10.1038/nature10625
  44. Zhang Y, Cao G, Qu LJ, Gu H. 2009. Involvement of an R2R3-MYB transcription factor gene AtMYB118 in embryogenesis in Arabidopsis. Plant Cell Rep. 28:337-346 https://doi.org/10.1007/s00299-008-0644-4
  45. Zhong R, Lee C, Zhou J, McCarthy RL, Ye ZH. 2008. A battery of transcription factors involved in the regulation of secondary cell wall biosynthesis in Arabidopsis. Plant Cell 20:2763-2782 https://doi.org/10.1105/tpc.108.061325
  46. Zhou J, Lee C, Zhong R, Ye ZH. 2009. MYB58 and MYB63 are transcriptional activators of the lignin biosynthetic pathway during secondary cell wall formation in Arabidopsis. Plant Cell 21:248-266 https://doi.org/10.1105/tpc.108.063321
  47. Zhu L, Shan H, Chen S, Jiang J, Gu C, Zhou G, Chen Y, Song A, Chen F. 2013. The heterologous expression of the Chrysanthemum R2R3-MYB transcription factor CmMYB1 alters lignin composition and represses flavonoid synthesis in Arabidopsis thaliana. PLoS One 19(6) e67680. DOI:10.1371/journal.pone.0065680