• The Korean Journal of Physiology and Pharmacology
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• 제27권6호
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• pp.521-531
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• 2023

Transmembrane protein TMEM16A, which encodes calcium-activated chloride channel has been implicated in tumorigenesis. Overexpression of TMEM16A is associated with poor prognosis and low overall survival in multiple cancers including lung adenocarcinoma, making it a promising biomarker and therapeutic target. In this study, three structure-related sesquiterpene lactones (mecheliolide, costunolide and dehydrocostus lactone) were extracted from the traditional Chinese medicine Aucklandiae Radix and identified as novel TMEM16A inhibitors with comparable inhibitory effects. Their effects on the proliferation and migration of lung adenocarcinoma cells were examined. Whole-cell patch clamp experiments showed that these sesquiterpene lactones potently inhibited recombinant TMEM16A currents in a concentration-dependent manner. The half-maximal concentration (IC50) values for three tested sesquiterpene lactones were 29.9 ± 1.1 µM, 19.7 ± 0.4 µM, and 24.5 ± 2.1 µM, while the maximal effect (E_max) values were 100.0% ± 2.8%, 85.8% ± 0.9%, and 88.3% ± 4.6%, respectively. These sesquiterpene lactones also significantly inhibited the endogenous TMEM16A currents and proliferation, and migration of LA795 lung cancer cells. These results demonstrate that mecheliolide, costunolide and dehydrocostus lactone are novel TMEM16A inhibitors and potential candidates for lung adenocarcinoma therapy.

• ETRI Journal
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• 제36권5호
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• pp.704-713
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• 2014

Most natural language processing tasks depend on the outputs of some other tasks. Thus, they involve other tasks as subtasks. The main problem of this type of pipelined model is that the optimality of the subtasks that are trained with their own data is not guaranteed in the final target task, since the subtasks are not optimized with respect to the target task. As a solution to this problem, this paper proposes a consolidation of subtasks for a target task ($CST^2$). In $CST^2$, all parameters of a target task and its subtasks are optimized to fulfill the objective of the target task. $CST^2$ finds such optimized parameters through a backpropagation algorithm. In experiments in which text chunking is a target task and part-of-speech tagging is its subtask, $CST^2$ outperforms a traditional pipelined text chunker. The experimental results prove the effectiveness of optimizing subtasks with respect to the target task.

• 한국전자거래학회지
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• 제13권3호
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• pp.51-66
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• 2008

기업이 비즈니스 프로세스의 중요성을 인식하게 되면서 비즈니스 프로세스 관리시스템에 대한 관심이 높아졌다. 그러나 전통적으로 BPMS는 업무프로세스의 모형화 및 실행에 그 초점이 맞추어져 있어, 상대적으로 프로세스 관리의 궁극적인 목적인 프로세스 효율성 제고나 더 나아가 프로세스를 혁신하는 데에는 큰 기여를 하지 못하였다. BPMS는 일반적으로 방대한 량의 이력 데이터를 남기게 되는데, 이에는 프로세스의 실행과 관련된 다양한 의미있는 정보나 규칙들이 숨어있다. 본 연구는 데이터마이닝 기법을 활용하여 프로세스 이력 데이터를 분석하는 체계적인 방법론을 제시한다. 제시된 방법론은 프로세스 분석을 위한 데이터 모형, 분석절차, 그리고 시스템 프레임워크를 포함한다. 또한 그 유용성을 검증하기 위하여, 시뮬레이션 데이터를 이용한 실험결과를 제시한다.

• 한국정보통신학회논문지
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• 제13권9호
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• pp.1787-1794
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• 2009

본 논문에서는 거실에서의 가족이 즐길 수 있는 IPTV 게임에 활용될 수 있으며, 유년기 자녀들의 게임 중독을 막을 수 있는 텐저블 전자 주사위 인터페이스를 제안한다. 제안된 텐저블 전자 주사위 인터페이스는 실제 주사위와 가능한 유사하게 설계되어 직접적이고 직관적인 조작으로 사용자들이 사용할 수 있도록 하였다. 제안된 인터페이스는 외부 장치를 필요로 하지 않는 자립식(self-contained) 장치이다. 실험을 통해 제안된 주사위 인터페이스가 PC나 IPTV 환경에서 동작하는 게임에 효과적으로 적용될 수 있음을 확인할 수 있다. 앞으로 이 장치를 현재 개발중인 IPTV 게임 등에 적용하여 실용성을 검증할 예정이다.

• Journal of information and communication convergence engineering
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• 제8권2호
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• pp.234-237
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• 2010

P300 component of the event-related potential(ERP) has been studied for theoretical, empirical, and clinical applications. Nowadays the 1-, 2-, and 3-stimulus oddball paradigms are used for eliciting P300 component of EEG in the auditory stimulus experiments. In this paper, we used a method to add one more stimulus to the 3-stimulus auditory paradigm. The adding stimulus has not the same volume but the same tone of the target stimulus. The 4-stimulus oddball paradigm to use two targets is used to elicit the P300 event-related potentials. In 4-stimulus oddball paradigm, an infrequent non-target (p=0.10) is presented in addition to two infrequent targets (p=0.10) and a frequent standard (p=0.70). Two target stimuli elicited a P300 component with a parietal maximum distribution. The amplitude of the P300 in target 2 was larger than that in target 1 and the latency of the P300 in target 2 was longer than that in target 1. The P300 component due to target 2 stimuli was larger than that due to target 1 stimuli. The experimental results approve that the 4-stimulus oddball paradigm can elicit P300 component clearly. The results are compared with the results of the traditional oddball paradigm.

• Biomolecules & Therapeutics
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• 제18권2호
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• pp.152-158
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• 2010

Melanin concentrating hormone is a neuropeptide highly expressed in the brain that regulates several physiological functions mediated by receptors in the G-protein coupled receptor family, especially plays an important role in the complex regulation of energy balance and body weight mediated by the melanin concentrating hormone receptor subtype 1 (MCH1). Compelling pharmacological evidence implicating MCH1 signaling in the regulation of food intake and energy expenditure has generated a great deal of interest by pharmaceutical companies as MCH1 antagonists may have potential therapeutic benefit in the treatment of obesity and metabolic syndrome. Although fluorescence-based calcium mobilization assay platform has been one of the most widely accepted tools for receptor research and drug discovery, fluorescence interference and shallow assay window limit their application in high throughput screening and have led to a growing interest in alternative, luminescence-based technologies. Herein, a luminescence-based functional assay system for the MCH1 receptor was developed and validated with the mitochondrial targeted aequorin. Aequorin based functional assay system for MCH1 presented excellent Z' factor (0.8983) and high signal-to-noise ratio (141.9). The nonpeptide MCH1 receptor antagonist, SNAP 7941 and GSK 803430, exhibited $IC_{50}$ values of 0.62 ${\pm}$ 0.11 and 12.29 ${\pm}$ 2.31 nM with excellent correlation coefficient. These results suggest that the aequorin based assay system for MCH1 is a strong alternative to the traditional GPCR related tools such as radioligand binding experiments and fluorescence functional determinations for the compound screening and receptor research.

• 센서학회지
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• 제15권2호
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• pp.77-83
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• 2006

A monitoring apparatus for pulse shapes of human heartbeats has been developed using an amorphous MI(Magnetic Impedance) sensor. The pulse shapes are successfully obtained from voltage signals due to the variations of magnetic impedance in the amorphous MI sensor, which is attached to a patient's wrist. This voltage signal was fed into a signal processing module to extract the pulse shapes of heartbeats. The signal processing module, which is proposed to detect a weak variations of impedance in MI sensor under a noisy measurement environment, consists of a high frequency current source, an amplifier stage and a synchronous detection circuit. To evaluate the characteristics of a newly developed apparatus, various experiments were performed. The experimental results show that the developed apparatus could be used as a diagnosis tool for traditional Korean medicine with further systematic clinical studies.

• 동의생리병리학회지
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• 제21권1호
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• pp.165-170
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• 2007

Pyungwi-san(PWS) have been using as a basic prescription of digestive disorder in Korean traditional medicine. This study was performed to examine the in vitro antioxidant activity of the extract using different antioxidant tests including by 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging, superoxide anion radical scavenging, metal chelating hydrogen peroxide scavenging, lipid peroxydation protective effect and scavenging effect of nitric oxide and peroxynitrite. Herbal-acupuncture solution of PWS(PWS-HS) exhibited a concentration-dependent inhibition of DPPH radical adduct formation and it showed dose-dependent free radical scavenging activity onto superoxide anions. In addition, the result of metal chelating hydrogen peroxide scavenging and ammonium thiocyanate experiments showed that PWS-HS was an active scavenger of hydroxyl radicals. Furthermore, it was also found to be effective in scavenging nitric oxide and peroxynitrite, well-known cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제14권1호
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• pp.101-105
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• 2008

본 논문은 전파 오류가 빈번한 802.11 무선 랜에서 VoIP(Voice over IP) 용량(capacity)을 해석적으로 계산할 수 있는 수학적 모델을 제안한다. 기존 모델은 오류가 발생하는 무선 채널에서의 VoIP의 용량을 시뮬레이션으로만 예측하였다. 본 논문은 전파 오류 환경에서 VoIP 용량을 해석적으로 평가하기 위해 기존 모델을 확장하여, 전파 오류, 전송 지연, 음성 품질, AP(Access Point)에서의 대기 시간 등의 영향을 포함하도록 하였다. 제안한 VoIP 용량 모델은 3%에서 9%까지의 오차 범위에서 시뮬레이션의 결과와 같다.

• Computers and Concrete
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• 제6권3호
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• pp.253-268
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• 2009

This study aims to develop a cost-based high-performance concrete (HPC) mix optimization system based on an integrated approach using artificial neural networks (ANNs) and genetic algorithms (GA). ANNs are used to predict the three main properties of HPC, namely workability, strength and durability, which are used to evaluate fitness and constraint violations in the GA process. Multilayer back-propagation neural networks are trained using the results obtained from experiments and previous research. The correlation between concrete components and its properties is established. GA is employed to arrive at an optimal mix proportion of HPC by minimizing its total cost. A system prototype, called High Performance Concrete Mix-Design System using Genetic Algorithm and Neural Networks (HPCGANN), was developed in MATLAB. The architecture of the proposed system consists of three main parts: 1) User interface; 2) ANNs prediction models software; and 3) GA engine software. The validation of the proposed system is carried out by comparing the results obtained from the system with the trial batches. The results indicate that the proposed system can be used to enable the design of HPC mix which corresponds to its required performance. Furthermore, the proposed system takes into account the influence of the fluctuating unit price of materials in order to achieve the lowest cost of concrete, which cannot be easily obtained by traditional methods or trial-and-error techniques.