• Applied Microscopy
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• v.28 no.3
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• pp.399-414
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• 1998

Glandular trichomes present on the leaf surface of Drosera capensis were examined using transmission electron microscopy. A large number of stalked glands exist on the adaxial surfaces of the leaf blade. The secretory head is composed of two layers of secretory cells, one layer of middle cells, and the inner tracheids. The secretory cells contain rough endoplasmic reticulum, mitochondria, plastids, Golgi apparatus, and vacuoles. The secretory cells show prominent cell wall ingrowth, and thick cuticle restricted on the subcuticular wall. Frequently, the cuticle has some pores, canal-like structures, showing electron -dense granules being penetrated through them. Ultrastructural localization using diaminobenzidine showed the electron-dense deposits in the vacuole. No peroxidase activity was seen in the cell wall and cytolasm. The activity of peroxidase (POX) isozymes in Drosera which isoelectric point (pI) is 3.6 and some anionic POX isozymes which pIs are laid between 3.6 and 4.6 were especially increased according to the development and the formation of glandular trichomes. Also, the activity of some POX isozymes which isoelectric points are laid between 4.6 and 5.1 were increased in the regions of leaves which has trichomes.

• Journal of the Korean Wood Science and Technology
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• v.21 no.1
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• pp.39-50
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• 1993

The purpose of this study was to describe the micromorphological changes in Korean red pine (Pinus densiflora S. et Z.) wood decayed by a major brown-rot fungus, Lentinus lepideus, using scanning electron microscope and transmission electron microscope. At the end of the 12-week exposure to the fungus in soil block procedure(ASTM 1971), test blocks sustained 5.02% weight loss. The formation of bore hole by hyphae and penetration of hyphae through bordered pit were not observed. Instead, fungal hyphae appeared to penetrate axially tracheid luminar from the the ray cells via cross field pits. Hyphae were mainly found in lignin rich cell corner regions of tracheids, and also extensive degradation of tracheid wall occurred in this region. Extensive degradation of $S_2$ layer occurred without noticeable alteration of the $S_3$ layer, but warty layer and compound middle lamella remained relatively intact. Localized erosion, the characteristic of white rot, was observed in some cell wall and wall components including lignin were found to be decomposed.

• Journal of the Korean Wood Science and Technology
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• v.16 no.2
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• pp.42-49
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• 1988

Anatomical characteristics on abnormal type of bisected compression woods and light colored wood intervened between these dark colored compression woods on under part and their respective opposite woods on upper parts of cross section in Pinus densiflora S. et Z. stem were compared through optical microscopic investigation. and the obtained results were summarized as follows: 1. Tracheid length increased linearly as the number of rings from the pith increases in all investigated parts, but increasing tendency in tracheid length was similar within bisected two compression woods and their opposite woods. 2. Tracheids were the shortest in bisected two compression woods and tracheid length in intervened wood between these two compression woods was longer than in compression woods but shorter than in the opposite woods. 3. Bisected two compression woods and intervened wood between these two compression woods revealed similar features in tracheid transition from springwood to summerwood, intercellular space, tracheid entanglement, and cross sectional view of tracheid. but spiral check was observed only in these two compression woods, therefore the intervened wood between these two compression woods disclosed anatomical characteristics similar to, not typical of, compression woods.

• Journal of Plant Biology
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• v.31 no.3
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• pp.169-185
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• 1988

Vessel elements in lateral root, tap root, transition region, stem and mid vein of 1-year old, 3-year old and 5-year old ginseng (Panax ginseng C.A. Meyer) are studied with light microscope to clarify the distribution and differentiation of several kinds of vessel elements. Vessel elements are classified into five types such as ring vessel, spiral vessel, scalariform vessel, reticulate vessel and pitted vessel according to the secondary thickenings of cell wall. All of the five types are not observed in each organ, but diverse kinds of vessels are present in stem and mid vein compared with the underground organs such as tap root and lateral root. The length of vessel elements is longest (680$\mu$m) in stem and shortest (143$\mu$m) in tap root. The diameter of vessel elements is 19.0$\mu$m in tap root and the angle of perforation plate comes under 22$^{\circ}$-60$^{\circ}$. The degree of differentiation of vessel elements according to the length, diameter and angle of perforation plate of vessel elements is highest in tap root regardless of the age of ginseng. Three types of perforation plate such as scalariform, intermediate type of simple and scalariform, and simple perforation plate are observed. The vascular tracheids are characteristically observed in mid vein of 1-year old ginseng, and in transition region of 3 and 5-year old ginseng.

• Journal of Forest and Environmental Science
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• v.30 no.2
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• pp.226-232
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• 2014

To understand transition characteristics from wood to charcoal the dimensional changes of carbonized woods at low temperature from $300^{\circ}C$ to $350^{\circ}C$ at the intervals of $10^{\circ}C$ were investigated. Three species of hardwoods and two species of softwoods were used in this study. Measurements of dimensional changes of cells were observed by stereoscopic microscope and an image analyzer. The apparent volume of each specimen decreased greatly with increasing temperature. Severe cracks and collapse were observed frequently in hardwoods and hardly in softwoods. Vessel diameter and tracheid cell wall thickness of the wood samples were decreased with increasing carbonization temperature. Contraction of vessel diameter in tangential direction was greater than that in radial direction. Cell wall thickness of tracheids decreased with increasing carbonization temperature. Consequently, even though it was small range of carbonization temperature, dimensions of wood components were changed considerably.

• Carbon letters
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• v.13 no.3
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• pp.161-166
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• 2012

Carbon materials were synthesized by pyrolysis from fibers of Corn-straw (Zea mays), Rice-straw (Oryza sativa), Jute-straw (Corchorus capsularis) Bamboo (Bombax bambusa), Bagass (Saccharum officinarum), Cotton (Bombax malabaricum), and Coconut (Cocos nucifera); these materials were characterized by scanning electron microscope, X-ray diffraction (XRD), and Raman spectra. All carbon materials are micro sized with large pores or channel like morphology. The unique complex spongy, porous and channel like structure of Carbon shows a lot of similarity with the original anatomy of the plant fibers used as precursor. Waxy contents like tyloses and pits present on fiber tracheids that were seen in the inherent anatomy disappear after pyrolysis and only the carbon skeleton remained; XRD analysis shows that carbon shows the development of a (002) plane, with the exception of carbon obtained from bamboo, which shows a very crystalline character. Raman studies of all carbon materials showed the presence of G- and D-bands of almost equal intensities, suggesting the presence of graphitic carbon as well as a disordered graphitic structure. Carbon materials possessing lesser density, larger surface area, more graphitic with less of an $sp^3$ carbon contribution, and having pore sizes around $10{\mu}m$ favor hydrogen adsorption. Carbon materials synthesized from bagass meet these requirements most effectively, followed by cotton fiber, which was more effective than the carbon synthesized from the other plant fibers.

• Journal of Forest and Environmental Science
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• v.30 no.3
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• pp.307-313
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• 2014

This study was conducted to distinguish the pines that are too similar to differentiate using conventional methods. Pinus densiflora and Pinus sylvestris have similar anatomical structure. They both have window-like pits and dentate ray tracheids, so it is not easy to distinguish the plants. We tried to find molecular markers by comparing chloroplast DNA sequences to differentiate the pines growing in Korea. We used P. densiflora, P. densiflora for. multicaulis, P. sylvestris, P. rigida, P. rigitaeda, P. koraiensis, and P. bungeana for this study. We found that the non-coding intergenic region of trnT(UGU) and trnL(UAA) genes have differences among the species. We designed a primer set to amplify the region efficiently and compared the PCR product sequences using CLC Workbench programs to find the polymorphism. We could distinguish the species using the sequences of the amplified region and the sequences were reproducible from the pines collected in Korea.

• Journal of Korean Society of Forest Science
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• v.10 no.1
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• pp.41-45
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• 1970

This study was carried out to investigate the identification of the woods of commercially important exotic tree species grown in Korea. The test trees used in this study were selected 21 species grown in Kwangyang and Suwon, Korea. The items of macroscopical features were observed and examined principally on the annual rings, transitions spring to the summerwood, pore types and arrangements, sap and heartwoods, wood colors, odor and tastes, resin ducts, parenchymas, and rays etc. The microscopical features observable in the elements, and their compositions such as vessels, tracheids, wood fibers, ray parenchyma cells, and intercellular cannals were observed and measured. The observed and measured results investigated were synthesized, and accordance with these results macroscopical and microscopical keys were prepared for the wood identification as seen in the text.

• Journal of Korean Society of Forest Science
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• v.80 no.3
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• pp.296-302
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• 1991

Compression wood and opposite wood formed in a branch of Taxodium distichum Rich. is described and compared in qualitative and quantitative anatomical aspects. The qualitative features of compression wood appeared to differ from those of opposite wood in very gradual tracheid transition from earlywood to latewood, roundish tracheid shape on cross surface, tracheid tip distortion on radial surface, and existence of intercellular spaces and helical cavities. In quantitative features, compression wood tracheids showed shorter lengths than opposite wood. The ray density and the number of uniseriate rays were greater in compression wood than in opposite wood but the height of uniseriate rays in compression wood was smaller than in opposite wood.

• Journal of the Korean Wood Science and Technology
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• v.15 no.2
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• pp.105-112
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• 1987

Some anatomical characterisitcs of Japanese red pine (Pinus densiflora S. et Z.) collected from pollution-exposed areas in Korea were investigated. These sites were reportedly the most severe air polluted areas in Korean peninsular. A decline in overall growth rate was apparent since the late 10-year period of growth. P. densiflora in Onsan and in Yeochon recorded in the radial growth reduction of approximately 70 and 60% respectively. The formation of earlywood in the period of air pollution was restricted, whereas the percentage of latewood increased. The maximum wood density was lower in xylem rings formed during a period of air-pollution than in rings found without pollution. Most of pine trees damaged by air pollution showed the classical type of length-on-age curve for tracheids. However, reduction in cell wall-thickness and diameter was revealed in the wood exposed to air pollutants. Nor anomalies in microstructures nor microorganisms in wood structures were found in the damaged trees.