• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2002년도 학술발표대회
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• pp.104-104
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• 2002

• 유기물자원화
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• 제13권3호
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• pp.89-96
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• 2005

본 연구에서는 유기인 화합물인 Paraoxon의 분해를 위하여 재조합 대장균에서 세포내 간극(periplasmic space)으로 분비되는 organophosphorus hydrolase(OPH)의 생산에 대하여 고찰하였다. OPH 생산의 향상을 위하여 성장 배지에 첨가되는 최적의 조건은 1.0 mM isopropyl-${\beta}$-D-thiogalactopytanoside (IPTG), 0.25 mM $Co^{2+}$ 및 0.1 mM ethylenediamine tetraacetate (EDTA) 이었다. 이 조건에서 최대OPH 생산은 $174Unit/L{\cdot}OD$를 나타내었다. 또한 1 mM의 Paraoxon은 OPH에 의하여 완전히 분해되었다. 이러한 연구 결과는 토양 및 수계에 잔류하는 환경독성 유기인 화합물을 제거하는 bioremediation의 수수단으로 활용될 수 있음을 보여주었다.

• BMB Reports
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• 제34권5호
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• pp.440-445
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• 2001

Pesticide waste and chemical stockpiles are posing a potential threat to both Vie environment and human health. There is currently a great effort toward developing effective and economical methods for the detoxification of these toxic organophosphates. In terms of safety and economy, enzymatic biodegradation has been recommended as the most promising tool to detoxify these toxic materials. To develop an enzymatic degradation method to detoxify such toxic organophosphorus compounds, a gene encoding organophosphorus acid anhydrolase (OPAA) from genomic DNA of Alteromonas haloplanktis C was subcloned and expressed. The enzyme consists of a single polypeptide chain with a molecular weight of 48 kDa. It demonstrates strong hydrolyzing activity on sarin, an acetylcholinesterase inhibitor. Moreover, its high activity is sustained for a considerable length of time. It is projected that the recombinant OPAA can be applied as an enzymatic tool that can be used not only for the detoxification of pesticide wastes, but also for the demilitarization of chemical stockpiles.

• Environmental Analysis Health and Toxicology
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• 제17권3호
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• pp.207-217
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• 2002

This study was designed to investigate the characteristics of atmospheric concentrations of toxic volatile organic compounds (VOCs) in Korea. Target compounds included 1,3-butadiene, aromatics such as BTEX, and a number of carbonyl compounds. In this paper, as the second part of the study, the seasonal and locational concentrations of atmospheric VOCs were evaluated. Sampling was conducted seasonally at seven sampling sites. each of them representing a large urban area (commercial and residential), a small urban area (commercial and residential), an industrial area (a site within the complex and a residential), and a background place in Korea. In general, higher concentrations were found in the petro-chemical industrial site than other sites, while VOCs measured in commercial (heavy -traffic) sites were higher than residential sites. Seasonality of VOCs concentrations were not so much clear as other combustion related pollutants such as sulfur dioxide, indicating that the VOCs are emitted from a variety of sources, not only vehicle exhaust and point sources but fugitive emissions. Except the industrial site, the concentrations of VOCs measured in this study do not reveal any serious pollution status, since the levels did not exceed any existing ambient standards in the U.K. and/or Japan. However, the increasing number of petrol -powered vehicles and the rapid industrialization in Korea may result in the increased levels of VOCs concentrations in many large urban areas in the near future, if there is no appropriate programme implemented for the control of these compounds.

• KSBB Journal
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• 제20권2호
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• pp.84-87
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• 2005

바이오디젤과 바이오디젤을 기반으로 생산된 neopentyl polyol ester (NPE)계 윤활유 베이스, 디젤유 윤활유와 석유디젤을 분석대상으로 하여 지렁이 (Eisenia fetida)를 이용한 독성 시험을 수행하였다. OECD 207규격에 근거하여 독성을 분석한 결과 바이오디젤과 NPE계 윤활유 베이스의 반수치사농도 $(LC_{50})$ 값은 각각 2,450과 1,528 mg/kg dry weight of soil로서 두 물질은 독성 이 적은 경독성 (slightly toxic) 물질인 것으로 판명되었다. 디젤유 윤활유와 석유디젤의 경우 반수치사농도 $(LC_{50})$ 값이 각각 500과 603 mg/kg로서 중등독성 (moderately toxic) 물질로 판정되었다.

• 공업화학
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• 제30권5호
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• pp.513-522
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• 2019

유기물질, 전이금속 및 유기-금속 구조체(MOFs)를 기반으로 하는 촉매들이 유기인 계열 독성물질들을 분해하고 제거하는데 효과적임이 보고되어 왔다. 최근 20년간 독성물질 분해연구를 위해 다공성 MOFs들이 응용 목적에 맞게 디자인되고 합성되었다. $Zr_6$ 기반의 금속노드와 유기결합체를 가지는 MOFs들은 세공크기, 공극률, 표면적, Lewis acidic 자리 그리고 열적 안정성 등과 같은 기본구조내의 변형이 가능하기 때문에 화학작용제, 살충제 및 제초제를 제거하는 촉매로 널리 사용되어왔다. 본 리뷰에서는 구조, 안정성, 입자크기, 연결된 리간드 수, 유기 기능기 등에 따른 MOFs들의 촉매효율과의 연관성을 다루게 될 것이다.

• 약학회지
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• 제34권2호
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• pp.102-105
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• 1990

The pyrolysis of mesaconitine and hypaconitine which are present as strongly toxic components in the raw aconite roots was performed. C(15)-keto pyro type compounds 1, 4 were obtained by the pyrolysis of mesaconitine and hypaconitine, respectively and structures of those compounds were determined as 16-Epi-pyromesaconitine(1) and 16-Epi-pyrohypaconitine (4) having $C(16)-{\alpha}-OCH_3$ on the basis of their spectral and chemical data.

• 한국군사과학기술학회지
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• 제5권3호
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• pp.105-112
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• 2002

A combined process of non-thermal plasma and catalytic techniques has been investigated to treat toxic gas compounds in air. The treated gas in the present study is $CH_3$CN that has been known to be a simulant of toxic chemical agent. A planar type dielectric barrier discharge(DBD) reactor has been used to generate non-thermal plasma that produces various chemically active species, O, N, OH, $O_3$, ion, electrons, etc. Several different types of adsorbents and catalysts, which are MS 5A, MS 13X, Pt/alumina, are packed into the plasma reactor, and have been tested to save power consumption and to treat by-products. Various aspects of the present techniques, which are decomposition efficiencies along with the power consumption, by-product analysis, reaction pathways modified by the adsorbents and catalysts, have been discussed in the present study.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2012년도 추계학술대회 논문집
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• pp.145-146
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• 2012

Recently the problem of the fouling organisms, especially Sn-compounds is expanded to environmental problems from the chemical reaction with imposex and secondary contamination. One of the existing antifouling system, antifouling paints, is regulated from 2012, July because of seriousness about toxic substances such as TBT, mercury, copper and so on. TBT is known that causes a variety of biological inhibition in various chemicals even a very small amount of concentration. So it has been developed to replace it. In this paper, we try to develope a new system with the environmental background and the research of the adhesion characteristics of fouling organisms using a low current, high frequency high voltage pulse power, not toxic compounds.

• 한국군사과학기술학회지
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• 제4권1호
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• pp.188-197
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• 2001

Organophosphorus acid anhydrolases(OPAA) catalyzing the hydrolysis of toxic organophosphates have been found in a variety of prokaryotic and eukaryotic organisms. Of the several kinds of OPAA that can degrade nerve agents, such as DFP, sarin and soman, a OPAA gene harbored in the chromosomal DNA of Alteromonas haloplanktis strain was subcloned in order to develope an enzymatic degradation method of toxic organophosphorus compounds. For this 1481 bp DNA fragment containing OPAA gene and its flanking regions has been synthesized through PCR using chromosomal DNA of A. haloplanktis strain. After subcloning and subsequent expression, crude OPA anhydrolase was prepared and assayed. It was shown that the OPAA had a very high hydrolytic activity on DFP. The specific activity of the enzyme was 1,110 $\mu$mole.$min^{p-1}.mg^{-1}$ protein. It seemed that OPAA with such a high hydrolytic activity may give a good prospects to its use, as a biodegradation tool, in detoxifying toxic organophosphorus compounds, such as pesticides and chemical stockpiles which are posing a potential threat to the field environment and human health.