• 한국연초학회지
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• 제23권1호
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• pp.45-52
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• 2001

This study was carried out to evaluate the uncertainty in the analysis of total alkaloids and reducing sugar content in flue-cured tobacco. The sources of uncertainty associated with the analysis of total alkaloids and reducing sugar were the weighing of sample, the preparation of extracting solution, the addition of extracting solution into the sample, the preparation of standard solution, the precision of calibration curve for standard solution, the reproducibility of analysis, and the determination of water content in tobacco, etc. For the calculating uncertainties, Type A of uncertainty was evaluated by the statistical analysis of a series of observation, and Type B by the information based on supplier’s catalogue and/or certificated of calibration. It was shown that the main source of uncertainty was caused by the calibration curve of standard solution, the reproducibility of analysis, the volume measurement of 1$m\ell$, and the purity of nicotine reference material in the preparation of standard solution. The uncertainty in the addition of extracting solution, the sample weighing, the volume measurement of 100$m\ell$, and the determination of water content of tobacco contributed relatively little to the overall uncertainty. The expanded uncertainty of total alkaloids and reducing sugar in flue-cured tobacco at 95% level of confidence was $\pm$0.12% and $\pm$0.54%, respectively.

• 한국식품영양학회지
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• 제30권1호
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• pp.112-119
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• 2017

In this study, we investigated and compared some chemical properties of Korean natural honeys and sugar-fed honeys for their quality characteristics. The natural honey samples were monofloral from chestnut and acacia flowers, and the sugar-fed honey samples were collected from honeybees feeding on sugar cane and sugar beet. The chemical properties of the honey samples, such as moisture, total protein, total lipids, ash, carbohydrate, minerals, vitamins, and free amino acids were determined. The moisture content was $18.5{\pm}0.9%$ in natural acacia honey, $17.2{\pm}0.9%$ in natural chestnut honey, $19.6{\pm}0.9%$ in sugar cane-fed honey, and $24.8{\pm}%$ in sugar beet-fed honey. The total protein and ash contents were the highest in natural chestnut honey. Maltose and sucrose were not detected in natural honeys but were detected at 2~7% in sugar-fed honeys. The vitamin, mineral, and free amino acids contents of natural honeys were higher than sugar-fed honeys. The natural chestnut honey is the highest in honeys. These results confirmed that the quality of natural honey was better than that of sugar-fed honey. Also, the vitamin, mineral, and free amino acids contents are potential characteristics for distinguishing between natural and sugar-fed honeys.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.192-192
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• 2022

Sweetpotato is rich in starch, which is converted to sugar during storage due to enzymatic hydrolysis. The sugar content of sweetpotato is a component related to taste and storability. In this study, the sugar content (fructose, glucose, maltose, sucrose and total sugar content) of 94 genotypes was evaluated and the GWAS (Genome-Wide Association Study) was conducted to search for candidate genes for sugar content. The fructose and glucose content were 0.2 ~ 8.8 and 0.2 ~ 9.4 g/100g, respectively. The maltose, sucrose and total sugar content were 0.2 ~ 9.1,3.2 - 30.0 and 7.9 ~ 40.2 g/100g, respectively. The fructose and glucose showed a positive correlation (0.98). The 94 genotypes were genotyped with genotyping-by-sequencing (GBS) and aligned against the reference genome sequences of sweetpotato. The GBS libraries from 94 genotypes were sequenced on an Illumina HiSeqXten system, and 1,339,892 SNPs (Single Nucleotide Polymorphism) were generated. Filtering for < 60% missing rate and > 0.05 minor allele frequency resulted in a total of 44,255 SNPs used in GWAS. The GAPIT (Genome Association and Prediction Integrated Tool) was used to conduct based on the mean of sugar content with a Bonferroni-corrected chromosome-wide significance threshold with a -logio(P) of 5.95. The significant SNPs were obtained with fructose (seven), glucose (six), maltose (four) and sucrose (nine). There were several genes related to sugar content around the significant SNPs such as sugar transport protein 8-like, probable galactose-1 -phosphate uridyltransferase-like and beta-amylase. These results will contribute to understanding of sugar content and conversion in sweetpotato.

• 한국연초학회지
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• 제28권1호
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• pp.1-8
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• 2006

To get the information of flue-cured leaf, the chemical constituents, quality indices, leaf color and the effects of climatic factors on the physicochemical properties of leaf produced from 2000 to 2004 crop years at 6 growing areas were analysed. The average leaf chemical contents for 5 years were as follows ; nicotine 2.22%, total sugar 28.0%, total nitrogen 1.89%, ether extracts 6.37% and chlorine 0.38%. The nicotine and total nitrogen contents were low while the total sugar were high as compared with KT&G recommended contents(nicotine ; $2.5{\sim}3.0%$, total nitrogen; $2.0{\sim}2.5%$, total sugar ; below 25.0%). The variations of physicochemical properties among crop years were high while those of growing areas relatively low. The nicotine content of leaf was negatively correlated to the rainfalls in June and July sugar content was negatively correlated to the average air temperature in June and July according to crop years. The orange colored leaves were produced under the drought and long sunshine weather condition while the lemon colored leaves were produced under the contrary condition according to crop years. Blending the different crop year's leaves in the proper way may be beneficial to produce of uniform and consistent cigarettes. It is considered that the increasing of nitrogen fertilizer or improving of nitrogen uptake may be available to increase the nicotine and nitrogen and decrease the total sugar contents of flue-cured leaf tobacco.

• 한국식품조리과학회지
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• 제7권1호
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• pp.27-34
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• 1991

This study attempted to set up reasonable extraction time of Omija that was put in water for the various components to soak out. Changes of free sugars, organic acids, reducing sugar, total acid and tannin in Omija with various extraction times were investigated (together with the analysis of each components in Omija fruit). 1. High Performance Liquid Chromatography showed fructose, glucose, and sucrose to be the major free sugars of the Omija fruit. Free sugars and reducing sugar value in Omija beverage increased gradually in according with the extraction time, and marked 75.6% per total free sugars and 82.1% per total reducing sugar at 12 hours. 2. Gas Chromatography showed lactic acid, oxalic acid, fumaric acid, levulinic acid, succinic acid, malic acid, citric acid and pyroglutamic acid to be the major organic acids of the Omija fruit. Organic acids and total acids value in Omija beverage increased gradually on proportion to extraction time, and marked 97.0% per total organic acids at 9 hours and 79.0% per total acids at 12 hours. 3. Tannin content in Omija beverage was increased when extraction time was longer but it showed a low percentage as compared with the reducing sugar and total acid. Tannin content marked 48.8% per total tannin at 12 hours. 4. Sensory evaluation revealed that !1 hours of extraction produced the best quality products based in taste, flavor, color and over-all acceptability, considering the data, it seems possible to conclude that the optimum of time for extraction of Omija to water is 9 hours.

• 한국연초학회지
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• 제7권1호
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• pp.57-65
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• 1985

This study was conducted to obtain the information for the difference of chemical characteristics in each tobacco growing districts and year. The results obtained were as follows 1. Difference of chemical characteristics in 6 districts: In case of flue-cured tobacco leaves, the produced leaf tobacco from Cheong Ju and Dae Jeon districts showed middle level in nicotine and total sugar contents, on the other hand, low nicotine and high sugar contents tobacco leaves were produced in An Dong and Jin Ju districts and high nicotine and low sugar contents of tobacco leaves in Su Won and Chun Cheon , respectively. In Burley tobacco leaves, the nicotine and total nitrogen contents of Chun Cheon districts product's were lower than those of Dae Jeon , Jeon Ju and Kwang Ju district's one. 2. Comparison of each tobacco produced year : The nicotine contents of five-cured and Burley tobacco leaves were the highest in 1981 and 1979, respectively. Also, as time goes to recent year, nicotine and total nitrogen contents were decreased and total sugar contents somewhat was increased .

• 생명과학회지
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• 제14권1호
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• pp.98-103
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• 2004

해조 또는 해조성분을 가수분해시키는 효소를 생산하는 미생물이 존재할 것으로 추정되는 논물에서 채취한 미생물군 시료에서 다시마분말 첨가 고체배지를 이용하여 순수배양을 실시하여 16균주를 분리하였으며, 이들 균주를 다시마분말 첨가 액체배지에서 3주간 배양하며, 1주 간격으로 전당과 환원당의 함량을 측정하여 추출율을 비교한 결과 배양 전 기간에서 30A412균주가 높은 추출율을 나타내었다. 30A412 균주를 제외한 나머지 15균주는 3주간의 배양으로도 그 추출율이 10%이하를 나타내었다. 30A412균주의 알긴산, 미역 및 다시마 분말 첨가 배지에서의 성장율은 배양 1주일 후부터 서서히 증가하였으며, 대조구보다 높은 성장율을 보였다. 알긴산, 다시마 및 미역 분해율은 배양기간이 경과함에 따라 증가하는 경향을 보였다. 균체 성장율과 분해율 모두 다시마 배지에서 가장 높은 값을 나타내었으며, 다음으로 미역배지, 알긴산 배지의 순이었다.

• 한국수산과학회지
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• 제36권6호
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• pp.563-567
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• 2003

Various bacterial strains were isolated from rice field ditch water, and their seaweed degrading activities were investigated. They were incubated in a liquid medium of sea tangle (Laminaria iaponica) and sea mustard (Undaria pinnatifida) powder for 3 weeks. Ratios of reduced sugar to total sugar of the liquid medium were measured once a week. Ratios of reduced sugar to total sugar of 27A311, 27C221 27A111 and 27B121 strains were highest. Accordingly, these four strains were incubated in 3 different liquid media of sodium alginate, sea tangle powder, and sea mustard powder for 3 or 4 weeks. The ratios of reduced sugar to total sugar and cell growth were measured once a week. Cell growth was higher in 27A111 and 27C221. Ratios of reduced sugar to total sugar was higher for 27C221 in the liquid mediums with sodium alginate and sea mustard powder, and for 27A111 in the liquid medium with sea tangle powder.

• 한국연초학회지
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• 제9권1호
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• pp.45-55
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• 1987

For some basic information in tobacco breeding, the modes of inheritance and heritabilities for the twelve agronomic and chemical characters were estimated in the study of eight varieties partial diallel set. Additive gene actions were significant for all characters except total nitrogen content and dominance gene effects were also significant for all characters. Yield, number of leaves per plant, leaf length and leaf shape index were inherited as partial dominance, and overdominance was detected for plant height, stem diameter, internode length, total alkaloid, total nitrogen and total sugar content. Dominant gene showed increasing effects in yield, plant height, stem diameter, internode length, leaf width and total sugar content, but showed decreasing effects in the other characters. The broad heritability was very high for all characters. and the narrow heritability was high in days to flower and yield, but low in internode length, total alkaloid, total nitrogen and total sugar content.

• Asian-Australasian Journal of Animal Sciences
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• 제26권2호
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• pp.218-226
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• 2013

This experiment was conducted to evaluate the intake, nutrient apparent digestibility and the effect of total collection days (two and four days) on apparent digestibility estimates for lambs fed diets containing sugar cane treated with calcium oxide (CaO). Eight Santa In$\hat{e}$s castrated male lambs with a $16.6{\pm}1.8$ kg body weight were used. The lambs were distributed in two $4{\times}4$ Latin squares, with four experimental periods of 14 d each. The animals were kept in 1.2 $m^2$ individual pens, and the intake and digestibility evaluations were performed during the last four days of each period. The diets were formulated to be isonitrogenous, containing 14% crude protein (CP), and presenting 70% sugar cane treated with 0, 0.75, 1.5 or 2.25% of CaO (as-fed basis), corrected with 1% urea, and 30% concentrate. The sugar cane with added CaO was chopped, treated, and offered to the animals after 24 h of storage. The sugar cane with CaO increased the DM, OM, CP, NDF, NDFap, TC, NFCap and TDN intake (kg/d), when compared to natural sugar cane, and produced the same intake expressed as a percentage of body weight (% BW). The NFCap digestibility of the CaO-treated sugar cane was inferior to the NFCap digestibility in natural sugar cane. There was a linear increase in the DM intake with the CaO-added sugar cane, but the DM and NDF digestibility and the TDN content decreased linearly. The chemical treatment of sugar cane with CaO increases the intake but does not improve the nutrient digestibility. Two days of total fecal collection were found to be sufficient to estimate the total apparent digestibility in lambs.