• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.57-62
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• 2008

Isoflavone content in various parts of six soybean cultivars and soybean sprout during germination was analyzed by high performance liquid chromatography. The parts analyzed were seed coat, cotyledon, and axis for seeds and whole sprout, root, hypocotyl, and cotyledon for sprout. Two cultivars, Aga3 which is known to have the smallest seed size and the highest isoflavone content among the Korean soybean cultivars and Pungsannamulkong which is the most widely being used as soy-sprout, were selected for sampling from 1 to 10 days after germination. At the same weight, the order of isoflavone content increased from seed coat to cotyledon to axis. The highest total isoflavone(isoflavone$\times$dry weight) content was observed in the cotyledon and the lowest in the seed coat. The cotyledon of the Aga3 variety had the highest total isoflavone content and the lowest was measured in the Pungsannamulkong variety. The highest total isoflavone content, $10,788{\mu}g/g$, was observed in whole sprouts(cotyledon+hypocotyl+root) on day 7 for Aga3. After day 7, there was a decreasing trend in isoflavone content as the germination period increased. Total isoflavone content in the cotyledon of Aga3 significantly increased after seed germination, whereas the isoflavone content in the cotyledon of Pungsannamulkong decreased. However, total isoflavone content in the root of both varieties increased while isoflavone content in the hypocotyls decreased after seed germination.

• 한국작물학회지
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• 제65권2호
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• pp.84-92
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• 2020

콩 발아기간 중 몇 가지 면역활성제(자스몬산, 키토산, 베타글루칸 및 게르마늄)와 녹차 및 인삼 추출물이 이소플라본 함량 증대에 미치는 영향에 대하여 알아보았다. 1. 대부분의 처리에서 발아시간이 경과함에 따라 이소플라본 함량이 높아졌는데 면역활성제 중에서는 키토산 처리가 이소플라본 함량 증대에 가장 큰 효과가 있었으며 약초추출물 처리는 효과가 미미하였다. 2. 품종별 이소플라본 함량은 모든 처리에서 대풍2호>우람>풍산나물콩의 순서로 높았으며 이소플라본 함량은 품종과 biotic 물질 처리간 상호작용도 영향을 미치는 것으로 판단된다. 3. 개별 이소플라본 종류별 함량을 분석한 결과 malonyl-glucoside>glucoside>aglycone>acetyl-glucoside 순서로 함량이 높았으며 발아가 진행되면서 aglycone 함량이 증가하는 경향이었다. 따라서 콩 발아기간 중 biotic 물질 처리는 어느 정도 이소플라본 함량을 증대시키며 품종과 물질처리간 상호작용 효과도 있음을 확인할 수 있었다.

• 한국작물학회지
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• 제64권2호
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• pp.89-101
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• 2019

The variation of content of 12 soybean seed isoflavone components was determined in the aglycone, glucoside, malonylglucoside and acetylglucoside groups of 44 Korean soybean cultivars grown in 2016 as well as in 2017. The total isoflavone content of the 44 cultivars averaged at $2935.4{\mu}g/g$ and was in the range of 950.6 to $5226.3{\mu}g/g$ for two years. Malonylglucoside group averaged at $2437.2{\mu}g/g$ with the highest proportion of isoflavone composition (83.0%). Significant differences were observed between cultivars, years and their interactions for both the total isoflavone and each composition group contents (P < 0.0001); however, no year-wise differences were observed for daidzein and genistin. The broad-sense heritability ($h^2$) within the set of 44 Korean soybean cultivars was as high as 0.93 for the total isoflavone content and was in the range of 0.8-0.92 for each composition group of isoflavone except for acetylglucoside. The total isoflavone content in cultivar group for soy-sprout was higher ($3850.4{\mu}g/g$) than that for the other cultivar groups of soy-paste and tofu ($3082.8{\mu}g/g$), black or green soybean cooked with rice ($2345.8{\mu}g/g$), and early maturity group ($1298.6{\mu}g/g$). The total isoflavone content of 'Sowonkong', a soybean cultivar for soy-sprout, was the highest ($5226.3{\mu}g/g$). In the cultivar group for soy-paste and tofu, the average isoflavone contents of 'Daepung', 'Daepung2ho', 'Saegeum', 'Uram', and 'Jinpung' were higher than $4000{\mu}g/g$. With the exception of small seeded cultivars with low isoflavone contents such as 'Sohwang' and 'Socheongja', the seed size and total isoflavone content were significantly negatively correlated in 2016 and 2017, respectively ($r=-0.47^{**}$ and $-0.49^{**}$). The number of days of growth from flowering to maturity did not affect the variations observed in isoflavone content.

• 한국작물학회지
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• 제51권spc1호
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• pp.146-151
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• 2006

고 isoflavone 함유 콩 유전자원 선발 및 품종육성의 기초자료로 활용하기 위해 국내 콩 주요품종 43계통 및 보존 유전자원 648계통의 isoflavone 함량을 분석한 결과를 요약하면 다음과 같다. 1. 한국산 콩 주요 43품종의 평균 isoflavone 함량은 daidzein이 $484.4{\pm}223.9{\mu}g/g$, genisteinol $448.0{\pm}156.2{\mu}g/g$, glyciteinol $84.9{\pm}48.7{\mu}g/g$, total isoflavoneol $1,017.3{\pm}390.0{\mu}g/g$을 나타내었다. 2. 공시된 주요 품종의 isoflavone 함량은 석양풋콩이 $2,002{\mu}g/g$으로 가장 높은 함량을 나타내었고, 황금콩이 $277{\mu}g/g$으로 가장 낮은 함량을 나타내었다. 또한 콩 주요 43품종을 용도별로 분류하여 isoflavone 함량을 비교한 결과 풋콩 및 올콩이 $1,438{\pm}493.7{\mu}g/g$을 나타내어 조사된 용도별 콩 분류 중 가장 높은 양상을 나타내었다. 3. 국내 보존 콩 유전자원 648계통을 대상으로 isoflavone 함량을 조사한 결과 IT 180001계통이 $3,479{\mu}g/g$의 함량을 나타내어 가장 높은 양상을 나타내었고, IT 910878 계통은 $82.61{\mu}g/g$의 total isoflavone을 함유하여 가장 낮은 계통으로 조사되어 보존 유전자원간에 큰 함량변이를 나타내었다.

• 한국작물학회지
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• 제51권spc1호
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• pp.174-178
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• 2006

본실험은 조생종인 하대두형 및 중국수집종 콩과 중숙${\sim}$만숙종의 국내 육성 종과의 isoflavone 함량변이를 비교하며, 파종기 이동 및 콩의 생육단계별 생육기간에 따른 isoflavone함량의 차이를 분석하고자 수행하였으며, 결과를 요약하면 다음과 같다. 1. 전체 isoflavone함량은 파종기 이동에 관련 없이 국내 수집종이 하대두형 및 중국수집 종에 비하여 놓았으며, 하대 두형콩과 중국수집 종과 비슷한 함량 분포를 나타냈다. 2. 파종기이동에 따른 총 isoflavone 함량은 파종기를 늦게 할수록 하대두형, 중국수집종 및 국내육성종 모두에서 증가하는 경향 이었다. 3. 콩의 개화기${\sim}$성숙기의 기간 및 생육일수가 길어질수록 isoflavone 함량도 높아지는 경향을 나타냈으며, 통계적으로도 부합되었다. 4. 콩의 isoflavone 함량은 입중이 무거울수록 함량이 낮아 졌으며, 상관계수는 4월${\sim}$5월 파종구에서 각각 -0.57, -0.51 및 -0.38로 분석되었다.

• 한국작물학회지
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• 제51권1호
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• pp.81-88
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• 2006

The 117 soybean cultivars were collected from nine provinces in Korea, and various seed quality traits along with isoflavone contents were evaluated to elucidate their relationship. The 100-seed weight of the black soybean (31.2 g) was significantly higher (p<0.05) than yellow soybeans (28.6 g). The composition of genistein, daidzein, and glycitein accounted for 75.8, 22.8, and 1.4 % of total isoflavone in yellow soybean cultivars, while their compositions in black soybeans were 58.5, 39.7, and 1.8%, respectively. The mean contents of total isoflavone in yellow and black soybean were $l,561.6{\mu}g\;g^{-1}\;and\;l,018.3{\mu}g\;g^{-1}$. The isofalvone content showed significant variation among cultivars when classified by the seed size. In the yellow soybeans, total isoflavone content was higher in small size soybean cultivars $(1,776.0{\mu}g\;g^{-1})$ and medium size soybean cultivars $(1,714.3{\mu}g\;g^{-1})$ compared to large size ones $(1,518.5{\mu}g\;g^{-1})$. Genistein content was proved as the major factor determining the relationship between isoflavone content and 100-seed weights (r =-0.206*). Daidzein and glycitein, however, showed no significant relationship with the 100-seed weights. Isoflavone content was not significantly correlated with color parameters L (lightness) and a (redness) values, but color parameter b (yellowness) was positively correlated with glycitein (r=0.264*) in the yellow soybeans, while its negative correlation between daidzein (r=-0.245*) and total isoflavone (r=-0.256*) were observed in black soybeans. However, these findings suggested that the seed color value may not serve as an effective parameter for estimating the isoflavone intensity of the soybeans. Variation of protein and lipid contents between yellow soybeans (n=58) and black soybeans (n=59) was relatively stable, however, protein and lipid contents have no significant relationship with isoflavone content.

• Food Science and Biotechnology
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• 제16권3호
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• pp.426-433
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• 2007

We analyzed the isoflavone content of domestic soybeans during steaming, boiling, fermentation, germinating cultivation, fermentation, and soybean curd production. The isoflavone content of the beans was reduced by steaming and boiling, and overall reductions ranging from 16.0 to 65.0% of initial isoflavone values were detected. After 4 days of germinating cultivation, the total isoflavones of Eunhakong increased from 1,341 to $2,017\;{\mu}g/g$ and the total isoflavones of Guinunikong increased from 1,284 to $1,535\;{\mu}g/g$. The isoflavone content of the vinegar beans produced from Hwangkeumkong and Black No.1 increased from 1,877 to $1,956\;{\mu}g/g$, and from 885 to $1,956\;{\mu}g/g$ after 8 days of immersion in 4% acetic acid, respectively. During soybean curd production, significant amounts of isoflavones were lost in the whey (30-31 %) and soybean curd residue (15-20%). Only 37.4% of the isoflavones present in the original soybeans remained in the soybean curd with the hot extraction method, and 50.7% of them with the cold extraction method. Soybean curd prepared with whole soybean method, however, retained 80.7% of the initial isoflavones.

• 한국식품과학회지
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• 제35권4호
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• pp.568-575
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• 2003

Isoflavone함량이 높은 우량나물콩 품종과 우량 콩나물을 선별하기 위해 우리나라의 나물콩 66품종과 이들로 재배한 30종 콩나물의 isoflavone함량을 분석하여 다음과 같은 결과를 얻었다. 66종 나물콩의 총 isoflavone의 평균 함량은 $1209.0{\pm}470\;mg/kg$, 함량범위는 $247{\sim}2,256\;mg/kg$이었고 daidzein과 genistein이 약 1:1의 비율로 함유되어 있었다. 66개 나물콩 품종 중 총 isoflavone 함량이 높은 상위 10개 품종은 KLG10618(2256 mg/kg), KLG11118(2233 mg/kg), KLG10600(1984 mg/kg), KLG10022(1982 mg/kg), KLG10855(1903 mg/kg), 소호콩(1843 mg/kg), KLG11078(1809 mg/kg), KLG11051(1792 mg/kg), KLG10783(1781 mg/kg) 그리고 KLG10873(1762 mg/kg)이었다. 시료 나물콩의 종피색별 총 isoflavone평균 함량은 녹색종에서 가장 높았고 종자크기별 층 isoflavone 평균 함량은 중립종($16{\sim}24\;g$/100립)에서 높은 편이었다. 30종 콩나물의 총 isoflavone 평균 함량은 1898.3 mg/kg이었고, 함량범위는 $768{\sim}3,343\;mg/kg$이었으며, 소호콩(3343 mg/kg), 명주나물콩(2943 mg/kg), KLG11051(2676 mg/kg), KLG11118(2650 mg/kg), KLG10650(2562 mg/kg) 순으로 높았다. 콩에서 콩나물로 재배시 총 isoflavone함량은 평균 1.4배 증가하였다. 콩나물의 총 isoflavone의 함량은 재배일수에 따라 증가하여 5일째에 가장 높은 함량 나타내었다. 콩나물의 부위별 총 isoflavone 함량은 뿌리, 자엽, 배축 순으로 높게 나타났고 daidzein의 함량은 뿌리에서 가장 높았고 genistein의 함량은 자엽에서 가장 높았다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.101-101
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• 2020

Soybean is known as to have a several healthy ingredients. Among them, isoflavones are effective in reducing obesity, menopausal symptom. Isoflavones consist of 12 isomers, including Aglycon, Glucoside, Malonyl glucoside, Acetyl glucoside, and are usually found in soybean seeds. The content is determined by the sum of 12 isomers, and the content value difference between the varieties is huge. In this study, we investigated the agronomic traits, 12 isomer of isoflavone content and composition for 49 soybean germplasms. This germplasms were selected from the 23,000 germplasms with the highest total content of isoflavones possessed by the National Agrobiodiversity Center. Seed samples were cultivated in experimental field located in Jeonju City on April 04, 2019. Matured seeds were harvested and portions of each seed samples were oven-dried, pulverized, and analyzed for their isoflavone compositions using HPLC-DAD. The soybean samples showed distinction in their agronomic traits, isoflavone compositions and contents. The days to flowering ranged between 38 and 69 days while the days to maturity ranged between 103 and 156 days. The seed coat color of soybean germplasms was 24 in black, 10 in yellow, 2 in green, 5 in yellowish green, 4 in green with black spot, 4 in pale yellow. The germplasm with the highest total content of isoflavones was the IT178054(1257.61±7.98 ㎍/g), but the germplasms containing the largest number of isoflavone isomers were IT274592, IT275005, both germplasms had 11 isoflavone isomers excluding Malonyl glycitin. The largest source of Aglycon, the most easily absorbed isoflavone form in the human body, was IT274592(DZ: 8.83±0.30 ㎍/g, GL: 11.14±0.81 ㎍/g, GE: 8.16±0.26 ㎍/g), while only IT274592, IT275005, IT308619 contained all three components of Aglycon. In Principal Component Analysis(PCA), the first two principal components showed more than 3.5 Eigen value and accounted for 58.2% of variability. The total content value had strong relationship with Malonyl genistin content value. Acetyl isomers had strong relationship, but Malonyl isomers were only related to isomers except Malonyl glycitin. These results will help in research on soybean varieties to enhance isoflavone ingredients.

• Journal of Nutrition and Health
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• 제35권4호
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• pp.409-413
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• 2002

성장기 수컷 흰쥐를 대상으로 콩단백질과 콩의 isoflavones의 함량이 골밀도 및 골함량에 미치는 효과를 알아본 결과는 아래와 같다. 1) 식이 효율은 대조군인 casein군에 비하여 콩단백질군이 낮았다. 2) 단백질의 종류나 isoflavones의 유무에 따른 혈 중이나 뇨 중 Ca과 P의 함량에는 유의적인 차이가 없었다. 3) 체중 당 총골밀도는 두 군의 콩단백질군이 대조군 보다 높았고, 콩단백질 내에서는 isoflavones를 함유한 군이 더 높았다. 4) 체중 당 spine의 골밀도나 골함량은 두 군의 콩단백질군이 대조군 보다 높았고, 콩단백질 내에서는 골밀도는 isoflavones를 함유한 군이 유의적으로 더 높았다. 5) 체중 당 femur의 골밀도는 콩단백질군이 대조군 보다 높았고, 콩단백질 내에서는 isoflavones를 함유한 군이 더 높았으나 통계적 유의성은 없었다. 결론적으로 콩단백질은 체중당 총골함량이나 칼슘함량에 있어서 동물성 단백질인 casein 보다 유익하였고 특히 isoflavones를 함유한 콩단백질은 체중당 총골밀도와 spine 골밀도에서 isoflavones 함량이 낮은 콩단백질에 비하여 더 유익하여 콩단백질과 isoflavones은 성장기 수컷 흰쥐에서 골밀도 향상에 유익함을 확인하였다. 따라서 성장기에 콩의 섭취는 casein 단백질에 비하여 골밀도나 골함량을 높이는데 유익하며 특히 isoflavones를 함유한 콩의 섭취는 골밀도를 높이는데 더욱 유익하다고 사료된다.