Analysis of Isoflavones for 66 Varieties of Sprout Beans and Bean Sprouts

66품종 나물콩과 콩나물의 isoflavone 분석

  • Kim, Youn-Hee (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Hwang, Young-Hyun (Department of Agronomy, Kyungpook National University) ;
  • Lee, Hye-Sung (Department of Food Science and Nutrition, Kyungpook National University)
  • Published : 2003.08.01

Abstract

Selection of high-quality varieties of sprout bean and bean sprout containing high levels of total isoflavones was performed by high performance liquid chromatography. The range and mean of total isoflavone contents of sixty six varieties of sprout beans were $247{\sim}2,256$ and $1209{\pm}470\;mg/kg$, respectively, with KLG10618, KLG11118, KLG10600, KLG10022, KLG1085 and sohokong containing the highest amounts of isoflavone among the samples. Medium-sized bean variety with green seed-coat color contained highest amount of total isoflavones among samples. The range and mean of total isoflavone contents of thirty varieties of bean sprouts were $768{\sim}3,343$ and $1,898{\pm}577\;mg/kg$, respectively, with soho bean sprout containing the highest total isoflavone content (3,343 mg/kg, dry basis). Total isoflavone contents of bean sprouts increased gradually during cultivation period, reaching maximum level on the 5th day of cultivation, and were the highest in the order of roots, cotyledon, and hypocotyl.

Isoflavone함량이 높은 우량나물콩 품종과 우량 콩나물을 선별하기 위해 우리나라의 나물콩 66품종과 이들로 재배한 30종 콩나물의 isoflavone함량을 분석하여 다음과 같은 결과를 얻었다. 66종 나물콩의 총 isoflavone의 평균 함량은 $1209.0{\pm}470\;mg/kg$, 함량범위는 $247{\sim}2,256\;mg/kg$이었고 daidzein과 genistein이 약 1:1의 비율로 함유되어 있었다. 66개 나물콩 품종 중 총 isoflavone 함량이 높은 상위 10개 품종은 KLG10618(2256 mg/kg), KLG11118(2233 mg/kg), KLG10600(1984 mg/kg), KLG10022(1982 mg/kg), KLG10855(1903 mg/kg), 소호콩(1843 mg/kg), KLG11078(1809 mg/kg), KLG11051(1792 mg/kg), KLG10783(1781 mg/kg) 그리고 KLG10873(1762 mg/kg)이었다. 시료 나물콩의 종피색별 총 isoflavone평균 함량은 녹색종에서 가장 높았고 종자크기별 층 isoflavone 평균 함량은 중립종($16{\sim}24\;g$/100립)에서 높은 편이었다. 30종 콩나물의 총 isoflavone 평균 함량은 1898.3 mg/kg이었고, 함량범위는 $768{\sim}3,343\;mg/kg$이었으며, 소호콩(3343 mg/kg), 명주나물콩(2943 mg/kg), KLG11051(2676 mg/kg), KLG11118(2650 mg/kg), KLG10650(2562 mg/kg) 순으로 높았다. 콩에서 콩나물로 재배시 총 isoflavone함량은 평균 1.4배 증가하였다. 콩나물의 총 isoflavone의 함량은 재배일수에 따라 증가하여 5일째에 가장 높은 함량 나타내었다. 콩나물의 부위별 총 isoflavone 함량은 뿌리, 자엽, 배축 순으로 높게 나타났고 daidzein의 함량은 뿌리에서 가장 높았고 genistein의 함량은 자엽에서 가장 높았다.

Keywords

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