• 한국물환경학회지
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• 제22권6호
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• pp.1075-1081
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• 2006

In this research, mortality rates of pathogen indicator microorganisms discharged from various point sources and diffuse sources in urban area were measured. Water samples were taken at domestic sewer, combined sewer overflow, effluent from a wastewater treatment plant, urban river, and sediment of an urban river. Mortality rates of indicator microorganisms in domestic sewer estimated by assuming the first order kinetics at $20^{\circ}C$ were as follows: total coliform = 0.092/day, fecal coliform = 0.185/day, E. coli = 0.252/day, and fecal streptococci = 0.281/day. Sensitivity of mortality rates of total coliform on temperature was estimated as $K_{temp}=K_{20}{\times}1.162^{(temp-20)}$ for the range of $10-20^{\circ}C$. Mortality rates due to sunlight were measured as 1.22-1.59/day while mortality rate due to settling for 40 min were estimated as $9.21{\times}10^3-20.0{\times}10^3/day$.

• 한국환경보건학회지
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• 제28권2호
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• pp.193-202
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• 2002

The polymerase chain reaction(PCR) of target lacZ and uidA genes were used to detect total coliform and Escherichia coli for determining water quality, respectively. Of 109 spring waters, coliform were detected from 38 spring waters by lacZ PCR method but 21 spring waters by culture method accepted by the Ministry of Environment for water quality monitoring. The lacz PCR method gave the results statistically equivalent to those of the culture method(kappa=0.62, McNemar=17.00). The uidA PCR method gave the same results to those of the culture method. The sensitivity and specificity of coliform and E. coli by PCR method were 100% and 80.7%, respectively. Therefore, PCR can be used for the rapid identification of Escherichia coli and coliform in potable water using uidA and lacZ.

• 한국환경보건학회지
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• 제33권5호
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• pp.422-427
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• 2007

The purposes of this study were to investigate the concentration of total coliforms in sewage effluents during the period from August 2004 to October 2005. The removal efficiency range of multi-tube method and plate count method were $31.3{\sim}99.5%$ and $66.8{\sim}99.2%$, respectively. Though a correlation between the multi-tube method and the plate count method in the same sample is low, not only is an experimental procedure very simple, but the time required also is short. The seasonal correlation between methods showed more sensitive spring and summer than autumn and winter. So the study indicated plate count method can be used in rapid and reliance identification of total coliform more than the multi-tube method.

• 환경영향평가
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• 제16권4호
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• pp.241-250
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• 2007

A study was carried out on the level of heavy metals, total coliform and benzo(a)pyrene that may be introduced through by-product fertilizers or air pollution to turfgrass in natural turfgrass playground in elementary schools around the western coast and eastern mountain areas in Jeollabuk-do, Korea. The level of heavy metals found in turfgrass was in the order of Cu > Zn > Pb > Cd. The majority of heavy metals was found in the roots. It is presumed that the absorption of some air pollutants and by-product fertilizers or chemical fertilizers. The level of benzo(a)pyrene showed an average of 0.05ng/g with a range between 0.02 to 0.08ng/g in the leaves. In the meantime, none was detected in the stems, while the average for roots was 0.02ng/g with a range between 0.01 and 0.03ng/g. The level detected is that found background level, however, benzo(a)pyrene can be introduced partly from the unstable combustion of fossil fuel or through vehicle emissions. Total coliform distributed in grass showed an average of 12MPN/100ml with a range between 2 and 36MPN/100ml. The density of total coliform displayed no regional predominance. However, in some urban areas, the density was slightly higher. The feces of pets or by-product fertilizers may have contributed to the input of pathogenic micro-organism. Yet the level was found to be negligible.

• 한국수산과학회지
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• 제54권6호
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• pp.1045-1051
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• 2021

Eighty-nine different types of commercially salt-fermented fishery products comprising various raw materials were analyzed for total aerobic bacteria, number of coliform bacteria, fecal coliform, and Escherichia coli. The food-poisoning bacterial content of the samples was investigated using next-generation sequencing. The mean mass of total aerobic bacteria in Jeotgal was 6-1.8×10⁹ CFU/g, and that in Aekjeot and Sikhae was 4-2.2×10⁵ CFU/mL and 1.9×10⁵-8.4×10⁸ CFU/g, respectively. Coliform bacteria were detected in 9 (28.1%) of 32 Jeotgal samples; 15 (46.8%) of 32 seasonal Jeotgal samples; and in 5 (55.5%) of 9 Sikhae samples. Fecal coliform and E. coli were not detected in 86 of the 89 samples. Yersinia enterocolitica was detected only in Galchi jeot (salt-fermented hairtail) (1 type) and not in other Jeotgal samples. These results contribute to our knowledge regarding the bacterial stability of salt-fermented fishery products.

• 한국환경보건학회지
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• 제35권3호
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• pp.162-168
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• 2009

The aim of this study is to investigate microbial sanitary condition of public baths in Seoul, Korea. A total of 28 water samples were collected from 14 different public baths and sudatoriums. The prevalence of fecal indicator microorganisms such as total coliform, fecal coliform, and Escherichia coli was characterized. In addition, bacteria in water was membrane filtered by 0.45um nitrocellulose membrane, and the filter was analyzed by both cultivation and PCR amplification of partial 16S rRNA gene. The levels of chlorine were measured for each of water samples. More than 40% of 14 collected water samples, the concentrations of total coliform bacteria exceeded the water quality for bath water guideline. There was no significant correlation between chlorine residue and the presence of total coliform. Various microorganisms including pathogenic microorganisms were identified from cultivation and subsequent analysis of 16s rRNA gene sequences. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bathhouses.

• 한국축산식품학회지
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• 제18권4호
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• pp.301-306
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• 1998

Four low fat ground beef groups containing 10% fat plus 0, 5, 10 and 20% additional cooked lod rice and a control ground beef containing 30% fat were prepared and the analysis for microbiological and oxidative stability were conducted. During 6 days of storage at 4$^{\circ}C$ microbial analysis including total plate count and coliform groups were performed and 2-thiobarbituric acid reactive substance (TBARS) absorbances were measured. The growth rate of total aerobic bacteria and coliform groups tended to increase with the increase in fat content and the amount of added cooked lod rice. Development of oxidative rancidity were not significantly different between 10 and 30% fat ground beef but among the 10% ground beef the rancidity development significantly(p<05) decreased with the in-crease in the amount of added cooked old rice. Low fat ground beef groups were not stable over 3 days during storage at 4$^{\circ}C$.

• 한국물환경학회지
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• 제22권3호
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• pp.504-512
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• 2006

In order to estimate microbial contaminant discharge from diffuse sources, rainfall runoff was monitored at forestry, agriculture and urban watersheds. Total coliform and E. coli were monitored at the study watersheds as they are regulated by the environmental laws. Concentration and EMC (Event Mean Concentration) of coliform of rainfall runoff at the urban watershed were the highest followed by those from agricultural and forestry watersheds. By monitoring coliform concentrations of overlying water and sediment at five monitoring points in the downstream of the Geum River, average concentration from spring to summer was higher than those values from fall to spring. Coliform concentrations in the pore water were higher compared to those of overlying water and closely related with flow rate of the river.

• 한국습지학회지
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• 제25권4호
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• pp.326-334
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• 2023

본 연구는 낙동강수계 지류에서의 장기 수질모니터링 자료를 이용하여 수질의 목표수질 초과율, 증가 추세를 검토하여 지류에서 문제 되는 물질이 무엇인지 파악하는 것이 목적이다. 낙동강수계에서는 중점 관리가 필요한 38개 지류에 대하여 월 1회 모니터링이 수행되고 있으며 이 자료를 이용하여 지점별 목표수질 초과 및 증가 경향을 분석하였다. 분석 항목은 하천 수질 기준으로 평가가 가능한 DO, BOD, COD, TOC, SS, 총인, 분원성 대장균군, 총대장균군 8개 항목이다. 분석 결과, 목표수질 초과율이 50% 이상이며 증가추세인 항목은 TOC, 분원성 대장균과 총대장균수로 나타났으며 초과율이 50% 이하이나 증가 경향을 가진 항목은 SS로 나타났다. TOC는 난분해성 물질의 증가로 인한 원인으로 판단되며 대장균의 지속적인 증가는 향후 하수처리시설에서의 방류수질 중 대장균의 관리가 필요할 것으로 판단된다.

• 한국농공학회지
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• 제45권7호
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• pp.94-106
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• 2003

A pilot study was performed to examine the feasibility of UV disinfection system and the reactivation of indicator microorganisms (TC, FC, E. coli) after UV irradiation for agricultural reuse of reclaimed water. Photoreactivation and dark repair enable UV-inactivated microorganisms to recover and may reduce the efficacy of UV inactivation, which might be drawbacks of the UV disinfection method. The effluent of biofilter for 16-unit apartment house was used as input to the UV disinfection system, and average SS and BOD concentration were 3.8 and 5.7 mg/L, respectively, and the mean level of total coliform was in the range of $1.0\times10^4$ MPN/100mL. UV disinfection was found to be effective and it reduced mean concentration of indicator microorganisms (total coliform, fecal coliform, and E. coli) to less than 100 MPN/100mL within 60s exposure using 17, 25, and 40W lamps. Two UV doses of 6 and 16 mW$\cdot$s/$\textrm{km}^2$ were applied and microorganisms reactivation was monitored under the dark, photoreactivating light, and solar irradiation. Microorganisms reactivation was observed in the UV dose of 6 mW$\cdot$s/$\textrm{km}^2$, and numbers increased up to 5% at the photoreactivating light and 1% at the dark. However, microorganisms were inactivated rather than reactivated at the solar radiation and numbers decreased to non-detectible level about below 2 MPN/100mL in 4 hours. In the case of 16 mW$\cdot$s/$\textrm{km}^2$, microorganism reactivation was not observed indicating that UV dose might affect the reactivation process such as photoreactivation and dark repair. Therefore, concerns associated with microorganism reactivation could be controlled by sufficient UV dose application. Agricultural reuse of reclaimed water might be even less concerned due to exposure to the solar irradiation that could further inactivate microorganisms. The pilot study result is encouraging, however, sanitary concern in water reuse is so critical that more comprehensive investigation is recommended.