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Detection of Coliform and Escherichia coli in Spring Water by Polymerase Chain Reaction  

류승희 (서울특별시 보건환경연구원)
박석기 (서울특별시 보건환경연구원)
Publication Information
Journal of Environmental Health Sciences / v.28, no.2, 2002 , pp. 193-202 More about this Journal
Abstract
The polymerase chain reaction(PCR) of target lacZ and uidA genes were used to detect total coliform and Escherichia coli for determining water quality, respectively. Of 109 spring waters, coliform were detected from 38 spring waters by lacZ PCR method but 21 spring waters by culture method accepted by the Ministry of Environment for water quality monitoring. The lacz PCR method gave the results statistically equivalent to those of the culture method(kappa=0.62, McNemar=17.00). The uidA PCR method gave the same results to those of the culture method. The sensitivity and specificity of coliform and E. coli by PCR method were 100% and 80.7%, respectively. Therefore, PCR can be used for the rapid identification of Escherichia coli and coliform in potable water using uidA and lacZ.
Keywords
Coliform; Escherichia coli; Spring water; Polymerase chain reaction;
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