• 생명과학회지
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• 제10권3호
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• pp.254-261
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• 2000

난소를 절제한 female rat에 Vit C. Vit E, Vit C와 Vit E를 복합적으로 투여했을 때 rat의 간 조직 의 손상 정도 및 항산화 효소 활성을 관찰하기 위해 체중 150$\pm$10g이 되는 female rat에 100mg/kg 함 량의 각 실험 물질들을 이틀에 한 번 두달 간 복강내로 투여한 뒤 간 조직 중 과산화지질량 및 항상화 효소인 SOD, catalase, 총 glutathion 량 및 Glutathion peroxidase의 활성도를 측정함으로써 항산화계의 변화 및 생리 활성 변화를 살펴 보았다. 1. 간, 신장 조직 중의 과산화지질 함량은 노화군(Ovx)이 정상 군(Sham)에 비해 각각 1.78배, 1.61배 증가하였다. 그리고 각 비타민 투여군은 난소를 절제한 군에 비해 과산화질함량이 현저히 감소하였다. 2. 혈청 중 총 cholesterol 량은 노화군(Ovx)이 정상군(Sham)에 비 해 2.57배 높게 나타났다. 각 비타민 투여군은 노화군에 비해 수치가 현저히 감소하였는데, Ovx+Vit C 군의 경우는 94%정도의 억제율을 보였다. HDL-cholesterol은 노화군(Ovx)이 정상군(Sham)에 비해 36.7% 감소하였다. 각 비타민 투여로 HDL-cholesterol 함량이 좋은 수준으로 증가되었고, Ovx+Vit Erns은 노화군보다 2.52배 높게 나타났다. 동맥경화지수도 Ovx+Vit E 군이 아주 낮게 나타났다. 3. 간 조직 중 SOD 활성도는 노화군(Ovx)이 정상군(Sham)보다 5.3배 높게 나타났고, 각 비타민 투여로 free radical 생성이 억제되어 항산화효소인 SOD 활성도를 감소시켰다. 간 조직 중 catalase 활성도는 노화군 (Ovx)이 정상군(Sham)보다 1.58배 정도 높게 나타났는데, 각 비타민 투여로 catalase 활성도가 낮아졌 고, Ovx+Vit E 군은 탁월한 감소 효과를 보였다. 4. 간 조직 중 총 glutathion의 활성도는 정상군 (Sham)이 노화군(Ovx)에 비해 1.22배 정도 높게 나타났고, 각 비타민 투여군 중 Ovx+Vit Erns의 경우 간 조직 중 총 glutathionfid이 가장 높게 나타났다. 간조직 중 reduced-glutathion (GSH)함량과 총 glutathion 량에 대한 oxidized-glutathion (GSSG)의 비율, 또한 Ovx+Vit E 군의 수치가 가장 높게 나 타났다. Vit E가 항산화에 좋은 효과가 있음을 알 수 있었다. 그러나, GPx의 활성 수치는 그 다지 유의 한 변화를 나타내지는 않았다.

• 한국가금학회지
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• 제41권1호
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• pp.61-68
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• 2014

본 시험은 육계에 야콘 부산물과 쑥 분말을 각각 0.5%와 1.0%를 급여하여 사육한 육계의 생산성과 다리 육의 도체특성, pH, TBARS, WHC, 전단력 및 육색을 조사하였다. 시험구는 야콘 부산물과 쑥 분말을 급여하지 않은 처리구를 대조구, 야콘 부산물 분말 0.5% 급여구는 T1, 야콘 부산물분말 1.0% 급여구는 T2, 쑥 분말 0.5% 급여구를 T3 그리고 쑥 분말 1.0% 급여구를 T4로 나누어 사양하였다. 야콘 부산물과 쑥 분말을 급여한 육계의 증체량, 사료섭취량 및 사료 요구율 등의 생산성의 유의적인 변화는 관찰되지 않았고, 폐사율은 유의하게 감소하였다. 혈액 생화학 성상 분석 결과, 야콘 부산물과 쑥 분말의 첨가 급여는 총콜레스테롤과 LDL-cholesterol 및 triglyceride는 감소하고, HDL-cholesterol은 증가하였다. 계육의 TBARS는 야콘 부산물과 쑥 분말을 급여함에 따라 낮아졌으며(P<0.05), 보수성과 전단력은 처리구간 유의적인 변화는 확인되지 않았다. 계육의 육색은 대조구보다 야콘 부산물과 쑥 분말 급여구에서 CIE $L^*$값은 낮아지고, CIE $a^*$값은 높아져 육색이 어두워지는 경향이었으며(P<0.05), CIE $b^*$값은 야콘 부산물과 쑥 분말의 급여에 의한 처리구 간의 유의적인 변화는 없었다. 결론적으로 야콘 부산물과 쑥 분말을 육계에 급여하면 TBARS 값이 낮아지고, 총 콜레스테롤과 LDL-cholesterol 및 triglyceride는 감소하며, HDL-cholesterol이 증가되어 콜레스테롤 대사에 도움이 될 것으로 사료되며, 이러한 경향은 야콘 부산물보다 쑥 분말 급여구에서 뚜렷한 결과를 나타내고 있다.

• 한국축산식품학회지
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• 제32권6호
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• pp.789-795
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• 2012

본 시험은 육계에 뽕잎 분말의 급여량에 따라(0, 1%, 2% 및 3%) 5주간 사육한 육계의 생산성과 육계의 도체특성, 혈액성상 및 육색을 조사하였다. 실험구는 뽕잎 분말을 첨가 급여하지 않은 처리구를 대조구, 뽕잎 분말 1% 급여구는 T1, 뽕잎 분말 2% 급여구는 T2 그리고 뽕잎 분말 3% 급여구를 T3 등 4개 처리구로 나누어 실시하였다. 육계의 생산성은 처리구간 유의적인 변화는 없었다. 도체특성 중 도체중, 도체율, 근위, 맹장, 심장 및 복강지방 무게는 처리구간에 유의성이 없었고, 간의 무게는 대조구와 T1 및 T2는 비슷하지만 T3에서 다소 가벼운 결과이었다(p<0.05). 육계의 혈액 성상 중 HDL-cholesterol과 glucose 및 albumin은 뽕잎 급여구에서 높았고, 총 콜레스테롤과 LDL-cholesterol은 낮아지는 결과로(p<0.05) 뽕잎을 3%이상 급여할 경우 유의한 변화를 보였다. 뽕잎 분말을 3% 급여한 계육의 $L^*$값과 $a^*$값은 증가하여 육색이 어두워지는 경향이었고, $b^*$값은 처리구간에 특이한 변화가 없었다. 결론적으로 뽕잎 분말을 3% 이상 급여하면 총콜레스테롤, LDL-cholesterol이 감소하며, HDL-cholesterol이 증가함으로 혈액성상을 개선할 가능성이 있는 것으로 판단된다.

• 동의생리병리학회지
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• 제21권1호
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• pp.104-110
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• 2007

Aralia contientalis show several beneficial effects including anti-oxidant effects. Aralia contientalis is used as a therapeutic agent to stop haemorrhages and a tonic to promoted health in Korean and Chinese medicine. The pharmacokinetic profiles of the main Aralia contientalis is still not accurately investigated. In present study, I examined the effects of water extracts of Aralia contientalis on high cholesterol diet atherosclerosis-induced rats in serum and abdominal aorta. A total of 3-week old 9 male rats of Sprague-Dawley were divided into 3 groups and fed with the basal diet (normal group), high cholesterol diet (atherosclerosis induced group) for 8 weeks, high cholesterol diet supplemented with water extracts of Aralia contientalis (Aralia contientalis group) for 2 weeks. And rats were sacrificed, serum lipid level, abodominal aortic anti-oxidant activities and lipid peroxide were measured. These results indicated that serum total cholesterl, LDL-cholesterol, triglycerides concentration significently lowered in Aralia contientalis group than high cholesterol diet group. But HDL-cholesterol concentraion significently higher in Aralia contientalis group than high cholesterol diet group.

• Nutritional Sciences
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• 제6권2호
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• pp.78-84
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• 2003

The objective of this study was to examine the effects of seven dietary oils on the serum lipid patterns of rats. Seventy weanling Wistar Kyoto rats were divided into seven groups of ten rats each. Walnut oil (rich in PUFA), wheat germ oil (rich in PUFA), corn oil (rich in PUFA), canola oil (rich in monounsaturated fatty acids), fish oil (rich in PUFA), primrose oil (rich in PUFA), and palm oil (rich in saturated fatty acids) were employed for 21 days. Serum total cholesterol concentrations for rats fed palm oil, walnut oil, and wheat germ oil were significantly higher than were concentrations for rats receiving corn oil. fish oil, and primrose oil. The mean serum LDL cholesterol values for rats fed fish oil, primrose oil, and corn oil were significantly lower than those for rats fed walnut oil, wheat germ oil, canola oil, and palm oil. HDL cholesterol concentrations were the highest when wheat germ oil was fed and the lowest when fish oil was fed. The feeding of wheat germ oil and palm oil to rats resulted in considerably higher serum triglyceride levels than did all other treatments. The feeding of wheat germ oil to rats resulted in considerably higher serum phospholipid levels. Serum phospholipid concentrations were significantly lower in rats fed the canola oil, fish oil, ,and primrose oil diets, when compared to concentrations achieved with the feeding of walnut oil, wheat germ oil, corn oil, and palm oil. Palm oil, which has a high ratio of saturated to polyunsaturated fatty acids, resulted in the highest serum total cholesterol and highest LDL cholesterol levels, while fish oil, primrose oil, and corn oil produced the lowest total cholesterol and LDL cholesterol. Wheat germ oil produced the highest values for HDL cholesterol, triglycerides, and phospholipids. In general, feeding oils rich in polyunsaturated fatty acids produced more favorable responses than feeding oils containing large amounts of monounsaturated or saturated fatty acids.

• Journal of Microbiology and Biotechnology
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• 제21권7호
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• pp.766-775
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• 2011

We investigated the effect of high molecular weight polygamma- glutamic acid (hm ${\gamma}$-PGA) on adiposity and lipid metabolism of rats in the presence of an obesity-inducing diet. Thirty-two Sprague-Dawley rats were fed either a normal-fat (11.4% kcal fat, NFC) or high-fat (51% kcal fat, HFC) diet. After 5 weeks, half of each diet-fed group was treated with hm ${\gamma}$-PGA (NFP or HFP) for 4 weeks. The HFC group had significantly higher body weight, visceral fat mass, fasting serum levels of total cholesterol, LDL cholesterol, and leptin, and lower serum HDL cholesterol level compared with those of the NFC group (p < 0.05). Treatment with hm ${\gamma}$-PGA decreased body weight gain and perirenal fat mass (p<0.05), fasting serum total cholesterol, and mRNA expression of glucose-6- phosphate dehydrogenase (G6PD), regardless of dietary fat contents (p < 0.01). However, hm ${\gamma}$-PGA increased serum HDL cholesterol in the HFC group (p < 0.05). In vitro, 3-hydroxy-3-methylglutaryl coenzyme-A (HMGCoA) reductase activity was suppressed by the addition of hm ${\gamma}$-PGA. In agreement with observations in animal study, the supplementation of hm ${\gamma}$-PGA (150 mg/day) to 20 female subjects in an 8-week double-blind, placebocontrolled study resulted in a tendency to decrease total cholesterol and LDL cholesterol concentrations. We thus conclude that dietary supplementation of hm ${\gamma}$-PGA may act as a hypocholestrolemic agent, secondary to its inhibitor effect on HMG-CoA reductase, and decrease abdominal adiposity by decreasing hepatic lipogenesis. The present study is an important first step in establishing the effect of hm ${\gamma}$-PGA on cholesterol levels in rats and humans.

• Journal of Nutrition and Health
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• 제37권4호
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• pp.273-280
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• 2004

This study was conducted to examine the effect of dietary iron levels on lipid metabolism, antioxidative and antithrombogenic capacities in 16-month-old rats. Thirty-two Sprague-Dawley male 16-month-old rats weighing 618 $\pm$ 6 g were raised for 10 days with medium-iron diet (35 ppm in diet) and blocked into 4 groups according to their body weights. One of groups was sacrificed to obtain initial data and the rest 3 groups were raised for 3 months with experimental diets containing different levels of iron (5 ppm, 35 ppm, and 350 ppm). Total lipid, triglyceride and total chole-sterol concentrations in plasma and liver, HDL-cholesterol concentration in plasma, fecal total lipid triglyceride and total cholesterol excretions, thiobarbituric acid reactive substances (TBARS) level in plasma LDL + VLDL (low density lipoprotein + very low density lipoprotein) fractions, blood-clotting time and eicosanoids levels in plasma were measured. The results are as follows: Plasma total lipid, triglyceride and total cholesterol concentrations, TBARS level in plasma LDL + VLDL fractions were increased and blood-clotting time tended to be shortened during 3 months of experimental period. Low (5 ppm) iron diet improved lipid metabolism via increasing HDL-cholesterol and fecal choles-terol excretion. High (350 ppm) iron diet decreased plasma total lipid, triglyceride and total cholesterol concentrations as compared to medium (35 ppm) iron diet and lowered body weight and epididymal fat pad weight. On the other hand, TBARS level in plasma LDL + VLDL fractions and blood-clotting time were increased with high iron diet. It is plausible that low iron diet improves lipid metabolism, antioxidative and antithrombogenic capacities in 16-month-old rats.

• 대한지역사회영양학회지
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• 제2권4호
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• pp.515-522
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• 1997

This study was done to compare serum levels of lipids, trace minerals, ceruloplasmin and ferroxidase activity between female college smokers and nonsmokers. Serum levels of lipids, Cu, Zn, ceruloplasmin and ferroxidase activity were determined in 33 smokers and 42 nonsmokers residing in the Seoul area. Serum levels of TG, total cholesterol and HDl-cholesterol were measured using test kits. Serum Cu Zn were determined by the AAS. Serum ceruloplasimin level and ferroxidase activity were determined by spectrophotometric methods. All data were statistically analyzed by the SAS PC package program. Mean and standard errors were calculated for each item, and the significance of the difference between two groups was evaluated by Student's t-test at p〈0.05. There was no significant difference in levels of total cholesterol, TG, VLDL-cholesterol, LDL-cholesterol, LDL/HDL and atherogenic index, but HDL-cholesterol level was significantly lower(p$\ll$0.05) in female college smokers than in nonsmokers. Serum zinc level showed no significant difference, whereas copper level was significantly higher in smokers, consequently yielding higher Cu/Zn ratio in smoking college women. Ceruloplasmin level was 32.6mg/dl in smoking college women, which was significantly higher(p$\ll$0.05) than that of 28.6mg/dl in nonsmokers. However, cerulopasmin ferroxidase activity, 0.34U/mg in nonsmokers was higher(p$\ll$0.05) than that of 0.29U/mg in smokers. From these results, it is confirmed that cigarette smoking itself has a very detrimental effect on health and mineral nutritional status although the smoking history is relatively short among college women(pack-years=1.7). (Korean J Community Nutrition 2(4) : 515-522, 1997)

• 동의생리병리학회지
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• 제18권2호
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• pp.431-435
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• 2004

The purpose of this study was to examine the effects of Salviae Miltiorrhizae Radix on the high fat diet-induced hyperlipidemia in rats. Sixty male Sprague-Dawley rats, around 270g of B.W., were divided into normal, control, sample A and sample B group. Normal and control groups were orally administrated with saline during 4 weeks, sample A and sample B with 252.5mg/kg(rat) and 420.1 mg/kg(rat) of solid extract of Salviae Miltiorrhizae Radix during 4 weeks respectively. Blood samples were collected from each of animals at 4 weeks, and used for the blood chemical analysis. Animals were sacrificed at 4 weeks, and chemical analysis was performed on the collected liver samples. The serum total cholesterol, HDL-cholesterol, LDL-cholesterol levels of sample A group were significantly decreased compared with the control group. On the basis of these of results, it is confirmed that Salviae Miltiorrhizae Radix has the curative effects on the hyperlipidemia in rats induced by high fat diet

• 대한약침학회지
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• 제7권3호
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• pp.47-57
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• 2004

Objective : In order to study effects of ${\lceil}Persicae\;Semen{\rfloor}$ on the hyperlipidemia rats induced by Triton WR, we have made hyperlipidemia rats by administering Triton WR-1339 for 3 days, then have administered. The results were summerized as follows : 1. The Total cholesterol, triglyceride, LDL-cholesterol, Free fatty acid, phospholipid and CRF(Cardiak Risk Factor) by hypercholesteremic rats injected by Triton WR-1339 of the herb-Acupuncture group were decreased as compared with those of the control group, especialy hypercholesteremic rats injected by Triton WR- 1339 of the herb-Acupuncture+Acupuncture group were some more decreased as compared with those of the control group. 2. The HDL-cholesterol by hypercholesteremic rats injected by Trion WR -1339 of the herb-Acupuncture group were increased as compeared those of control group, especialy hypercholesteremic rats injected by Triton WR-1339 of the herb-Acupuncture+Acupuncture group were some more increased as compared with those of the control group. According to the above results, Persicae Semen showed inhibition on decrease of serum total cholesterol, triglyceride and phosholipid contents as well as inhibition on increase of HDL-cholesterol contents in hyperlipidemia.