• 한국식품과학회지
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• 제54권2호
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• pp.147-154
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• 2022

본 연구에서는 몽골 야생 베리 추출물의 총 페놀성 화합물, 플라보노이드, 그리고 안토시아닌 함량과 항산화 효과를 측정하였다. 전구 염증매개 사이토카인의 유전자 발현에 미치는 영향을 살펴본 결과, LBE와 BBE의 전처리가 LPS 자극에 의해 증가된 TNF-α, IL-1β, iNOS, 및 COX-2의 mRNA 발현의 농도를 유의적으로 감소시키며 항염증 효능을 보여주었다. 또한 LBE와 BBE는 NOX2의 발현 억제를 통하여 활성산소의 생성을 저해시킴으로 세포내 염증성 산화적 스트레스를 감소시킴을 확인하였다. 몽골야생 블루베리에서는 6종의 안토시아닌이 검출되었으며, 야생 링곤베리에서는 1종의 안토시아닌이 검출되었다. 본 연구결과를 토대로 몽골의 야생 베리가 강력한 항산화 및 항염증을 통한 육식위주의 식단을 소비하는 몽골인들의 산화적 스트레스 및 만성 염증성 질환을 줄여주는 식품으로의 가능성을 확인하였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.111-111
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• 2018

Aronia (black chokeberry), a species of berries is source to a very large number of bioactive compounds like polyphenols, flavonoids, anthocyanins, and tannins in comparison to any other species. Owing to its antioxidant, anti-carcinogenic, anti-aging and anti-inflammatory properties. Fermentation- a bioconversion process exploiting the biological metabolic reaction of micro-organisms, has several benefits like improving the efficacy and safety of physiologically active substances, generation of new functional material, improving the adsorption rate and many others. Antigens like pathogens, food, pollen etc., generate a protective immune response in body tissues, and the process be referred to as inflammation. However, this when excessive results in a condition referred to as refractory inflammatory disease, whose incidence is increasing in the recent times, especially amongst children. The current study intended to assess the anti-oxidant activity, presence of polyphenols and anti-inflammatory efficacy of Aronia extract fermented by Lactic acid bacteria isolated from fermented sea foods. Aronia fruits collected from Sunchang, Chonbuk were lyophilized for fermentation. So as to maximise the efficacy of the fermented Aronia extract, the quantitative effects of lactic acid bacteria species, composition of extraction solution, influence of temperature and time on antioxidant activity and total polyphenol contents were investigated using an experimental design. Anti-inflammatory activity was evaluated on NO and cytokine ($TNF-{\alpha}$, IL-6) production in LPS activated Raw 264.7 cells. Results indicated that antioxidant effect and total polyphenol contents were best improved in extract of Aronia fermented by P. pentosaceus. In addition, NO and cytokine ($TNF-{\alpha}$, IL-6) levels were decreased significantly after fermentation. Thus, it was found that the anti-inflammatory activity of Aronia greatly increased after fermentation process using P. pentosaceus.

• 농업과학연구
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• 제46권4호
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• pp.971-980
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• 2019

We profiled the health-promoting bioactive components in nine types of soybean seeds with different seed coat colors (yellow, green, brown, and black) and investigated the effects of different extraction solvents (methanol, ethanol, and water) on their antioxidant activities. The carotenoid and anthocyanin compositions varied greatly by seed color, and the phenolic acids, total phenol, and total flavonoid contents differed by genotype. The carotenoid content was relatively higher in soybean seeds with green and black seed coats than in those with a yellow seed coat while lutein was the most plentiful. The anthocyanin content was considerably higher in the soybean seed with the black seed coat. The results of the DPPH assay showed strong antioxidative activities in the methanol- and water-extracts compared to the ethanol-extract, irrespective of the seed coat colors. Moreover, the soybean seeds with the black seed coat exhibited the highest antioxidant activity among the samples, regardless of the extraction solvent used. Eighteen bioactive compounds were subjected to data-mining processes including principal component analysis and hierarchical clustering analysis. Multivariate analyses showed that brown and black seeds were distinct from the yellow and green seeds in terms of the levels of carotenoids and anthocyanins, respectively. These results help our understanding of the compositional differences in the bioactive components among soybean seeds of various colors, providing valuable information for future breeding programs that seek to enhance the levels of compounds with health benefits.

• 한국약용작물학회지
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• 제24권3호
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• pp.183-190
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• 2016

Background: This study determined the effects of mulching, an environment friendly organic cultivation method, on antioxidant compound contents and growth in Codonopsis lanceolata, commonly known as Deodeok. Methods and Results: C. lanceolata was treated by mulching with several different methods (a non-woven fabric, biodegradable film, or rice husks) and also treated with hand weeding. A non-treatment plot was used as a control. The growth and levels of weed control in C. lanceolata were better in plants cultivated under mulching treatments (non-woven fabric, biodegradable film, and rice husks) than in those under non-mulching treatments (hand weeding and non-treatment). The contents of antioxidant compounds, such as total flavonoids, phenolics, and anthocyanins, were highest under the biodegradable film treatment, followed by the non-woven fabric treatment, rice husks treatment hand weeding, and non-treatment. There were identifiable differences in DPPH and ABTS activity in comparison to antioxidant compound content by solvent fractions. Mulching treatments resulted in higher DPPH scavenging activity in water and ethyl ether fractions and ABTS scavenging activity in n-butanol fractions than in other fractions, as opposed to hand weeding and non-treatment groups, although total activity of DPPH and ABTS did not increase with mulching treatments. Conclusions: Mulching C. lanceolata with biodegradable film and non-woven fabric is an effective method for improving plant growth and inhibiting the occurrence of weeds as well as for increasing antioxidant compound content and altering antioxidant activity.

• 동아시아식생활학회지
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• 제26권1호
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• pp.80-87
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• 2016

본 연구는 '설향'과 '장희' 두 품종의 품종별, 성숙도별에 일반 성분분석과, 항산화 물질함량, 항산화능을 비교하였다. 미숙과가 완숙과보다 경도가 더 높았으며, '설향'이 '장희'보다 단단하였다. 당도에서는 품종별의 차이를 보이지 않았으나, 과일이 성숙이 되면서 당도는 증가하였다. 산도는 미숙과가 완숙과보다 높게 측정되었으며, 과일이 완숙이 되었을 때는 '설향'보다 '장희'의 적정 산도가 더 높게 측정되었다. 총 페놀화합물 함량과 플라보노이드 함량에서는 '설향'이 '장희' 품종보다 더 우수하게 나타냈으며, 총 페놀화합물 함량의 경우 미숙과와 완숙과와는 유의차가 없었다. 총 안토시아닌 함량은 두 품종 모두 성숙과가 많은 양을 나타내었고, '장희' 품종이 '설향'보다 더 높게 나타났다. 항산화능에서는 DPPH 라디칼 소거능과 ABTS 라디컬 소거능 모두 '설향'이 '장희' 품종보다 더 우수하게 나타났다. 안토시아닌을 제외한 항산화 물질함량과 총 항산화능과는 매우 높은 상관 관계를 나타내었다. 본 연구 결과를 종합해 볼 때, 딸기의 당산비 등을 고려했을 때 완숙과를 섭취하는 것이 관능적으로 우수할 것으로 판단되나, 품종에 따라서 미숙과의 항산화 물질 및 항산화능이 높은 경우도 있어서 이러한 미숙과나 재배 중 발생하는 적과, 태풍 등 자연 환경변화에 따른 낙과를 이용한 다양한 기능성 원료의 개발도 충분히 가치가 있다고 사료된다.

• 한국축산식품학회지
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• 제33권6호
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• pp.806-816
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• 2013

복분자 와인을 첨가한 요구르트의 배양 중 변화는 모든 시험구에서 pH는 배양 시간이 지날수록 낮아지는 변화를 보였으며, 적정산도와 점도는 점차적으로 증가하는 경향을 보였다. 유산균수의 변화는 모든 시험구에서 시간이 지날수록 유산균수가 증가하는 경향을 나타 내었으며 5.0% 첨가구에서 가장 많은 유산균 수를 나타내었다. 저장 중 변화는 모든 시험구에서 저장 기간이 길어질수록 pH값이 점차 낮아졌으며 시간이 경과할수록 첨가구보다는 대조구에서 더 낮은 pH값을 나타내었다. 적정산도의 변화는 5.0%, 3.0% 첨가구가 1.0% 첨가구와 대조구보다 낮은 적정산도값을 나타내었고 점도의 변화는 0일에는 첨가구보다 대조구의 점성이 높았으나 6일 이후에는 대조구가 모든 첨가구의 점성보다 낮았다. 유산균수의 변화는 저장기간 내내 대조구보다 첨가구에서 높은 유산균수를 나타내었으며, 복분자 와인을 첨가한 요구르트의 알코올 함량, 안토시아닌 색소 함량과 TPA함량은 복분자 와인의 첨가량이 많을수록 수치도 높게 나타났다. 관능검사 결과, 첨가량이 증가할수록 색감과 조직성에서 선호도가 증가하는 것을 나타내었다. 복분자 와인을 첨가한 요구르트 제조 시험에서 5.0% 첨가구가 pH, 유산균 수, 점도와 안토시아닌 색소 회수 함량, total phenolic 함량과 관능평가에서 가장 우수한 성적을 보였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.318-318
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• 2022

Basil(Ocimum basilicum L.) is an annual herb that grows wild in hot and humid regions such as tropical Asia and Africa. This study was carried out for resource discovery, propagation, and DB construction of aromatic plants. In order to evaluate the genetic characteristics, 28 kinds of basil seeds were supplied from the Center for Genetic Resources. For basil characteristics, planting date, plant height, growth shape, leaf length, leaf shape, petiole color, petiole, stem color, flower color, after harvest, Ml length including roots, flower length, stem thickness and biological weight were measured. For anthocyanin analysis, only basil with purple color was selected, anthocyanins were extracted with 60% ethanol containing 1% citric acid, a standard quantitative curve was prepared with cyanidin-3-glucoside, and absorbance was measured at 525 nm. Basil planting started around June 16, and the flowering period lasted for a total of 18 days from July 19 to August 5. On August 6, when flowering was completed, morphological characteristics including flower color were measured at the site by an object. The measured basil plant length was 26.6-59.6 cm, leaf length 2.0-7.9 cm, leaf width 0.7-5.2 cm, and petiole 1.3-3.9 cm. The growth pattern of basil was generally straight, medium in shape. The color of the leaves was observed evenly by mixed green, purple, purple and green, and the recesses in the shape of the leaf were also conspicuously observed. A total of three basil flower colors were observed: white, light pink(149P 10P/84), and purple(37V 2.5RP 4/12). During the second growth survey after harvest, the total length including the basil roots is 26.7-52.0 cm, the flower length is 8.2-29.3cm, the stem thickness is 7.1-15.9mm, the number of stem nodes is 3-12 nodes, and the total weight of the basil was measured to be about 218-1540 g. There are a total of 16 types of basil expressed in purple, and the anthocyanin content was measured to be 73.74 ~ 380.72 mg/100g.

• 한국식품영양과학회지
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• 제36권4호
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• pp.499-502
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• 2007

본 연구에서는 쌀 섭취의 중요성에 착안하여 건강 기능성 쌀로 개발된 특수미의 항산화 성분을 분석하고자 하였다. 특수미의 polyphenol, phytic acid, anthocyanin은 비색법으로 vitamin E는 순상 HPLC로 분석하였다. 본 연구 결과 일반백미에 비해 특수미는 그 종류에 따라 약 $2{\sim}9$배의 높은 total polyphenol 함유하는 것으로 확인되었다. 또한 쌀에는 insoluble polyphenol이 total polyphenol의 약 $25{\sim}47%$를 차지하는 것으로 나타났다. 쌀의 vitamin E 함량은 백미(0.23mg ${\alpha}-TE/100g$)에 비해 현미(1.02mg ${\alpha}-TE/100g$) 및 특수미 $(0.97{\sim}1.21mg\;{\alpha}-TE/100 g)$에서 높은 함량을 보였고 phytic acid 함량은 백미, 현미, 거대배아미, 녹미, 적색미, 고아미, 흑미 순으로 각각 529, 1,429, 1,507, 1,561, 1,732, 2,163mg/100g으로 나타났다. 일반미와 특수미의 anthocyanin 함량을 측정한 결과 흑미 (302mg/100g)를 제외한 나머지 시료에서는 검출되지 않았으며 일반미와 특수미의 anthocyanin 함량 비교를 위해서는 HPLC 등을 이용한 정밀한 방법이 적용되어야 하겠다. 본 연구 결과는 쌀의 항산화 성분과 항산화 활성 연구에 있어 기초 자료로서 활용될 것으로 예상되며, 건강증진 식품으로서의 쌀을 인식시켜 나아가 쌀의 소비 촉진에 상당한 영향을 미칠 것을 기대된다.

• Journal of Dairy Science and Biotechnology
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• 제35권3호
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• pp.176-183
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• 2017

Flaxseed (Linum usitatissimum L.) is an important food, oil, and fiber crop of the family Linaceae. Although flaxseed has been consumed as a food ingredient for several centuries, its nutritional benefits have not yet been completely established. Flaxseed is a good source of lignans, nonstarch polysaccharides, and high-quality proteins. Hence, in this study, we aimed to prepare a bioactive kefir containing flaxseed and to examine the physicochemical characteristics of kefir containing different concentrations of flaxseed. We investigated the pH, and sensory evaluation of bioactive Kefir containing different concentrations of flaxseed. We investigated the pH, total anthocyanins (TAs), and sensory evaluation of bioactive Kefir containing different concentrations of flaxseed. The pH of this bioactive kefir decreased, whereas the TA content increased with increasing incubation time; however, these parameters were not affected by the amount of added flaxseed. As the addition rate of flaxseed increased, the scores for overall acceptability, texture, color, flavor, and taste in sensory evaluations were generally the same as or lower than the control. There were no significant differences in overall acceptability, texture, color, flavor, and taste between the control and treated groups. Therefore, further studies are needed to develop methods for production of health-improving kefir as a dietary supplement based on the functional properties of flaxseed.

• 한국작물학회지
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• 제51권2호
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• pp.173-178
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• 2006

Anthocyanin in blackish purple rice is composed of cyanidin, peonidin, malvidin, pelargonidin and delphinidin flavylium ion and their glucosides, which shows antioxidant activity similar to a-tocoperol. It has been demonstrated that cyanidin-3-glucoside(C3G) of anthocyanin has a high-potency antioxidant activity, and is a prominent in anticancer and antithrombotic activity. In analysis of content of anthocyanin from the genetic source of blackish purple rice, the level of C3G was approximately 80% of total contents of anthocyanins, and showed the highest content in Heugjinjubyeo, of which contains an amount of 500 mg by weight of 100g brown rice in comparison that most of varieties has less than 50mg by weight of 100g brown rice. We found that C3G pigment in blackish purple rice was considerably affected by cultural season, cultivation area, ripening temperature, and weather condition, etc. as well as genetic properties. In cross combination between Heugjinjubyeo and Suwon425, intermediate parent $F_9$ generation which was three times higher or more in C3G content than that of Heugjinjubyeo was bred, of which it contained an amount of 1678 mg by weight of 100g brown rice, and we called it 'C3GHi' Rice. And, we found that the C3GHi rice was more excellent than that of the existing Heugjinjubyeo in anticancer and antithrombotic activity. Methanol extract from Heugjinjubyeo was fractionated by organic solvents in order of n-hexane, $CH_{2}Cl_2$ and n-BuOH, and then oryzafuran, quercetin, vanillic acid and protocatechuic acid and their structures from the n-BuOH fraction were ascertained. Oryzafuran of these compounds was a natural compound found firstly in nature. Quercetin and protocatechuic acid besides new compound oryzafuran showed excellent antioxidant ability to vitamin C. These results suggest that blackish purple rice has very high value as a source of various functional food as well as staple food.