Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Analysis of antioxidant and anti-inflammatory effects of Mongolian wild lingonberry and blueberry, and identification of their bioactive compounds

몽골 야생 링곤베리와 블루베리의 항산화, 항염증 효과 및 생리활성 물질 분석 연구

  • Lee, Hye Ju (Department of Smart Green Technology Engineering, Pukyong National University) ;
  • Naranbulag, Batdorj (Department of Food Science and Nutrition, Pukyong National University) ;
  • Jeong, Seung Jin (Department of Smart Green Technology Engineering, Pukyong National University) ;
  • Seo, Chan (Department of Food Science and Nutrition, Pukyong National University) ;
  • Lee, Sang-Gil (Department of Smart Green Technology Engineering, Pukyong National University)
  • 이혜주 (부경대학교 스마트그린기술융합공학과) ;
  • ;
  • 정승진 (부경대학교 스마트그린기술융합공학과) ;
  • 서찬 (부경대학교 식품영양학과) ;
  • 이상길 (부경대학교 스마트그린기술융합공학과)
  • Received : 2022.02.11
  • Accepted : 2022.02.23
  • Published : 2022.04.30
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Abstract

The Mongolian lingonberry and blueberry are two essential food sources found in Mongolia. This study investigated the antioxidant and anti-inflammatory effects of methanol extracts from Mongolian lingonberry (LBE) and blueberry (BBE). Compared to the LBE, the BBE showed higher total phenolic, flavonoid, and anthocyanin contents, as well as antioxidant capacities. The LBE and BBE inhibited the mRNA expression of pro-inflammatory genes, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and cyclooxygenase (COX-2) in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. In addition, the LBE and BBE inhibited NADPH oxidase-2 (Nox2) mRNA expression, indicating that they have cellular antioxidant capacities. Anthocyanin derivatives of the LBE and BBE were analyzed using LC-QTOF/MS. Six anthocyanins were identified in the BBE, while one was detected in the LBE. Our findings demonstrate that the anthocyanin-rich LBE and BBE could be used as functional food sources in Mongolia.

본 연구에서는 몽골 야생 베리 추출물의 총 페놀성 화합물, 플라보노이드, 그리고 안토시아닌 함량과 항산화 효과를 측정하였다. 전구 염증매개 사이토카인의 유전자 발현에 미치는 영향을 살펴본 결과, LBE와 BBE의 전처리가 LPS 자극에 의해 증가된 TNF-α, IL-1β, iNOS, 및 COX-2의 mRNA 발현의 농도를 유의적으로 감소시키며 항염증 효능을 보여주었다. 또한 LBE와 BBE는 NOX2의 발현 억제를 통하여 활성산소의 생성을 저해시킴으로 세포내 염증성 산화적 스트레스를 감소시킴을 확인하였다. 몽골야생 블루베리에서는 6종의 안토시아닌이 검출되었으며, 야생 링곤베리에서는 1종의 안토시아닌이 검출되었다. 본 연구결과를 토대로 몽골의 야생 베리가 강력한 항산화 및 항염증을 통한 육식위주의 식단을 소비하는 몽골인들의 산화적 스트레스 및 만성 염증성 질환을 줄여주는 식품으로의 가능성을 확인하였다.

Keywords

Acknowledgement

본 논문은 부경대학교 자율창의학술연구비(2021)에 지원을 받아 연구되었으며 이에 감사드립니다.

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