Browse > Article
http://dx.doi.org/10.9721/KJFST.2022.54.2.147

Analysis of antioxidant and anti-inflammatory effects of Mongolian wild lingonberry and blueberry, and identification of their bioactive compounds  

Lee, Hye Ju (Department of Smart Green Technology Engineering, Pukyong National University)
Naranbulag, Batdorj (Department of Food Science and Nutrition, Pukyong National University)
Jeong, Seung Jin (Department of Smart Green Technology Engineering, Pukyong National University)
Seo, Chan (Department of Food Science and Nutrition, Pukyong National University)
Lee, Sang-Gil (Department of Smart Green Technology Engineering, Pukyong National University)
Publication Information
Korean Journal of Food Science and Technology / v.54, no.2, 2022 , pp. 147-154 More about this Journal
Abstract
The Mongolian lingonberry and blueberry are two essential food sources found in Mongolia. This study investigated the antioxidant and anti-inflammatory effects of methanol extracts from Mongolian lingonberry (LBE) and blueberry (BBE). Compared to the LBE, the BBE showed higher total phenolic, flavonoid, and anthocyanin contents, as well as antioxidant capacities. The LBE and BBE inhibited the mRNA expression of pro-inflammatory genes, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and cyclooxygenase (COX-2) in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. In addition, the LBE and BBE inhibited NADPH oxidase-2 (Nox2) mRNA expression, indicating that they have cellular antioxidant capacities. Anthocyanin derivatives of the LBE and BBE were analyzed using LC-QTOF/MS. Six anthocyanins were identified in the BBE, while one was detected in the LBE. Our findings demonstrate that the anthocyanin-rich LBE and BBE could be used as functional food sources in Mongolia.
Keywords
Mongolian wild blueberry; Mongolian wild lingonberry; antioxidant; anti-inflammation; anthocyanins;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bedard K, Krause K-H. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol. Rev. 87: 245-313 (2007)   DOI
2 Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal. Biochem. 239: 70-76 (1996)   DOI
3 Agarwal S, Piesco N, Johns L, Riccelli A. Differential expression of IL-1β, TNF-α, IL-6, and IL-8 in human monocytes in response to lipopolysaccharides from different microbes. J. Dent. Res. 74: 1057-1065 (1995)   DOI
4 Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 181: 1199-1200 (1958)   DOI
5 Giusti MM, Wrolstad RE. Characterization and measurement of anthocyanins by UV-visible spectroscopy. Curr. Protoc. Food Anal. Chem. F1. 2.1-F1. 2.13 (2001)
6 Hwang JH, Ma JN, Park JH, Jung HW, Park YK. Anti-inflammatory and antioxidant effects of MOK, a polyherbal extract, on lipopolysaccharide- stimulated RAW 264.7 macrophages. Int. J. Mol. Med. 43: 26-36 (2019)
7 Rice-Evans C, Halliwell B, Lunt G, Davies KJ. Oxidative stress: the paradox of aerobic life. Biochem. Soc. Symp. 61: 1-31 (1995)   DOI
8 Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-942 (1987)
9 Dudonne S, Dube P, Anhe FF, Pilon G, Marette A, Lemire M, Harris C, Dewailly E, Desjardins Y. Comprehensive analysis of phenolic compounds and abscisic acid profiles of twelve native Canadian berries. J. Food Compost. Anal. 44: 214-224 (2015)   DOI
10 Liu J, Hefni ME, Witthoft CM. Characterization of flavonoid compounds in common Swedish berry species. Foods. 9: 1-13 (2020)
11 Wu X, Prior RL. Systematic identification and characterization of anthocyanins by HPLC-ESI-MS/MS in common foods in the United States: fruits and berries. J. Agric. Food Chem. 53: 2589-2599 (2005)   DOI
12 Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559 (1999)   DOI
13 Kovac S, Angelova PR, Holmstrom KM, Zhang Y, Dinkova-Kostova, AT, Abramov, AY. Nrf2 regulates ROS production by mitochondria and NADPH oxidase. Biochim. Biophys. Acta.-General Subjects. 1850: 794-801 (2015)   DOI
14 Lee SG, Kim B, Yang Y, Pham TX, Park Y-K, Manatou J, Koo SI, Chun OK, Lee J-Y. Berry anthocyanins suppress the expression and secretion of proinflammatory mediators in macrophages by inhibiting nuclear translocation of NF-κB independent of NRF2-mediated mechanism. J. Nutr. Biochem. 25: 404-411 (2014)   DOI
15 Paixao N, Perestrelo R, Marques JC, Camara JS. Relationship between antioxidant capacity and total phenolic content of red, rose and white wines. Food Chem. 105: 204-214 (2007)   DOI
16 Rankin JA. Biological mediators of acute inflammation. AACN Adv. Crit. Care. 15: 3-17 (2004)
17 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237 (1999)   DOI
18 Kalt W, Ryan DA, Duy JC, Prior RL, Ehlenfeldt MK, Vander Kloet S. Interspecific variation in anthocyanins, phenolics, and antioxidant capacity among genotypes of highbush and lowbush blueberries (Vaccinium section cyanococcus spp.). J. Agric. Food Chem. 49: 4761-4767 (2001)   DOI
19 Hokkanen J, Mattila S, Jaakola L, Pirttila AM, Tolonen A. Identification of phenolic compounds from lingonberry (Vaccinium vitisidaea L.), bilberry (Vaccinium myrtillus L.) and hybrid bilberry (Vaccinium x intermedium Ruthe L.) leaves. J. Agric. Food Chem. 57: 9437-9447 (2009)   DOI
20 Hutabarat R, Xiao Y, Wu H, Wang J, Li D, Huang W. Identification of anthocyanins and optimization of their extraction from rabbiteye blueberry fruits in Nanjing. J. Food Qual. 2019: 1-11 (2019)
21 Kaur C, Kapoor HC. Anti-oxidant activity and total phenolic content of some Asian vegetables. Int. J. Food Sci. Technol. 37: 153-161 (2002)   DOI
22 Landete J. Dietary intake of natural antioxidants: Vitamins and polyphenols. Crit. Rev. Food Sci. Nutr. 53: 706-721 (2013)   DOI
23 Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB. Reactive oxygen species in inflammation and tissue injury. Antioxid. Redox Signal. 20: 1126-1167 (2014)   DOI
24 Rao X, Huang X, Zhou Z, Lin X. An improvement of the 2ˆ (-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat. Bioinforma. Biomath. 3: 71-85 (2013)