• Korean Journal of Ichthyology
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• v.21 no.1
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• pp.1-6
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• 2009

The effect of water temperature on fasting and post-prandial total ammonia excretion (TAN) of the starry flounder Platichthys stellatus (mean body weigh : $42.4{\pm}3.4g$) was studied. The fasting and post-prandial TAN excretions were measured under three different water temperatures (10, 15 and $20^{\circ}C$) for 24 hours using a recirculating system. In each treatment three replicates were set up and total 45 fish were used. Fish were taken to the indoor aquarium, acclimated over 10 days at three temperatures, and transferred to TAN measuring system for measurements of TAN excretion at the same temperatures. After 3 days of starvation, fasting TAN excretion was measured at each temperature. To investigate post-prandial TAN excretion, fish were hand-fed to satiation level with a commercial diet containing 50.2% crude protein for 7 days, two times daily 08 : 00 and 16 : 00 h. The fasting and postprandial TAN excretion increased with increased water temperature (p<0.05). Mean fasting TAN excretion rates at 10, 15 and $20^{\circ}C$ were 10.9, 11.2 and $12.2mg\;TAN\;kg\;fish^{-1}\;h^{-1}$, respectively. The value at $20^{\circ}C$ was higher than those at 10 and $15^{\circ}C$ (p<0.05), but there was no significant difference between $10^{\circ}C$ and $15^{\circ}C$. Mean post-prandial TAN excretion rates at 10, 15 and $20^{\circ}C$ were 33.0, 43.4 and $55.3mg\;TAN\;kg\;fish^{-1}\;h^{-1}$, respectively. Two peaks of post-prandial TAN excretions were observed, and the second peak was always greater than the first. The post-prandial TAN excretion rate reached to the maximum after 10 hours from the first feeding at $10^{\circ}C$($45.3mg\;TAN\;kg\;fish^{-1}\;h^{-1}$), $15^{\circ}C$ ($64.5mg\;TAN\;kg\;fish^{-1}\;h^{-1}$) and $20^{\circ}C$ ($83.2mg\;TAN\;kg\;fish^{-1}\;h^{-1}$), respectively. The TAN loss for ingested nitrogen at $20^{\circ}C$ (48.8%) was higher than that for $10^{\circ}C$ (43.0%) and $15^{\circ}C$ (45.7%). This study provides empirical data for estimating ammonia excretion and managing culture management of starry flounder under given temperatures.

• Korean Journal of Environmental Biology
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• v.26 no.3
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• pp.138-144
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• 2008

The effects of salinity on oxygen consumption, operculum movement number and ammonia nitrogen excretion were examined in cultured tilapia, Oreochromis mossambicus (total length 10$\pm$1.0 cm, total weight 17.3$\pm$4.2 g). The fish were exposured to certain salinities (0, 3, 9, 15, 21, 27 and 32 psu) step by step with 3 psu$\cdot$d$^{-1}$ and changed rapidly from certain salinity to another salinity. The oxygen consumption of the fish which was transferred step by step showed increasing tendency in the range of 3 $\sim$ 27 psu, but markedly appeared very low value at 32 psu. The tolerance limit in tilapia by salinity change without acclimation was shown 23.3 psu from 96-h TL$_{50}$. The oxygen consumption, operculum movement and ammonia nitrogen excretion of fish which was transferred rapidly from certain salinity to each salinity (0, 9, 15, 21 and 32 psu) showed a changing point at 15 psu; they showed increasing and/or decreasing pattern before 15 psu, and showed decreasing pattern after 15 psu. From these results, it was concluded that the appropriate salinity without physiological change for Java tilapia was below 15 psu.

• Journal of Animal Science and Technology
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• v.49 no.4
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• pp.491-500
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• 2007

Two experiments were used to determine the effects of environment-friendly diets on growth performance, fecal excretion, nitrogen excretion and emission gases in manure for growing pigs. In experiment 1, ninety six crossed pigs(Landrace×Yorkshire×Duroc) were allocated into four treatments. Treatments were AME(adequate ME diet, 3,265 kcal/kg), LME(lower ME diet, 3,100 kcal/kg), LME 0.05(lower ME diet+α- galactosidase & β-mannanase 0.05%) and LME 0.10(lower ME diet+α-galactosidase & β-mannanase 0.10%). Pigs fed AME diet had lower ADFI(Average Daily Feed Intake) than pigs fed other diets(p<0.05). DM(Dry Matter) digestibility in pigs fed AME and LME 0.10 diets had greater than pigs fed LME diet(p<0.05). Energy digestibility is higher in pigs fed AME and LME 0.10 diets than other treatments(p<0.05). In experiment 2, twenty four crossbred pigs(33.71 kg average BW) were used in a 14-d metabolism experiment. The pigs were housed in individual cages equipped with plastic bed flooring. Treatments were CP(Crude protein) 18% without Bacillus sp., CP 18% diet+Bacillus sp. 0.05%, CP 14% without Bacillus sp. and CP 14% diet+Bacillus sp. 0.05%. Nitrogen intake was higher for CP 18% diets than CP 14% diets(p<0.05). DM, N(Nitrogen) and energy digestibility were affected by probiotics(p<0.05). With the high CP in diets, Energy and N digestibility, urine N percent, urine N excretion and total N excretion were increased significantly compared to low CP in diets(p<0.05). Among the treatments, DM and N digestibilities, feces N excretion, N absorption were decreased significantly(p<0.05), however, feces excretion, feces N, urine N percent, urine N excretion and total N excretion were increased significantly(p<0.05) when pigs fed without probiotics diets compare to pigs fed with probiotics diets. DM and N digestibility, feces excretion, feces N excretion, urine N percent, urine N excretion, total N excretion, N absorption and N adsorption ratio were CP×probiotic interactions in p<0.05. Ammonia(p<0.01) and H2S(p<0.05) in manure were lower in CP 14% diets than CP 18% diets. Also, ammonia and H2S in manure were CP×probiotic interactions in p<0.05. In conclusion, low energy and reduction of CP dietary added enzyme and probiotics improved nutrient digestibility and reduced odors emission in manure for growing pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.1
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• pp.51-56
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• 2017

Objective: The objective of this study was to evaluate the intake and nutrient digestibility, nitrogen balance and ruminal ammonia nitrogen in lambs of diets containing different levels of residual frying oil. Methods: Levels of 0, 20, 40, 60, and 80 g/kg dry matter (DM) base of residual frying oil in the diets of lambs were evaluated. Five castrated lambs with initial body weights of $36.8{\pm}3.3kg$, distributed in a Latin square ($5{\times}5$) design, were used. Results: There was a decreasing linear effect on the intake of DM, organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), total carbohydrates (TCH), and nonfibrous carbohydrates (NFC). There was an increased linear intake of ether extract (EE). The apparent digestibility of DM, OM, CP, NDF, TCH, and NFC, as well as urine nitrogen excretion, nitrogen balance and ruminal parameters, were not influenced by different levels of residual frying oil in the diet. EE digestibility presented a crescent linear effect. Conclusion: It can be concluded that the addition of residual frying oil to the diets of sheep can affect nutrient intake without affecting the digestibility of most nutrients (with the exception of EE), nitrogen balance and ruminal ammonia nitrogen concentration.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.25 no.5
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• pp.371-382
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• 1992

Biochemical composition, the rates of nitrogen excretion in the form of ammonia, amino acids and total nitrogen, and oxygen consumption of the shrimp Crangon affinis were measured at three temperature regimes $(7,\;15,\;and\;25^{\circ}C)$ and the variations were also determined for starvation periods (1-25 days). The composition of the major biochemical fractions was analysed into carbohydrate: $4.2\%,\;protein:\;68.6\%,\;lipid:\;7.0\%,\;chitin:\;6.3\%,\;and\;ash:\;14.6\%,$ all expressed as percentage of dry weight. Molting frequency was lower at $7^{\circ}C\;than\;25^{\circ}C$ during the period of starvation, and during the same period the higher temperature was, body weight and body compositions the more decreased. Through all starvation periods $O_2$ consumption tended to decrease but total nitrogen tended to increase at any temperature regimes. The dominant form of excreted total nitrogen was ammonia-N at any temperature. From the O:N ratio it appeared that carbohydrate and lipid reserves were quickly exhausted (1-5 days), and that proteins were the substrates oxidized to meet the energetic requirements of C. affinis at any temperature. After 25 days of starvation the O:N ratio remained constant near a value of 8, which indicates that only proteins were being utilized at three temperatures. After 25 days of starvaion C. affinis excreted 23.01ug N/mg body nitrogen per day at $7^{\circ}C,\;32.97\mu g\;N/mg$ body nitrogen per day at $15^{\circ}C,\;and\;44.81\mu g\;N/mg$ body nitrogen per day at $25^{\circ}C$, and lost about 1.75, 2.47 and $3.29^{\circ}C$ of body protein per day at 7, 15, and $25^{\circ}C$ respectively.

• Korean Journal of Poultry Science
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• v.12 no.1
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• pp.17-24
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• 1985

In order to investigate an effect of non-protein nitrogen on the biological utilization of protein, hatched single comb White Leghorn male chicks were fed for the first 8 days with a commercial chicks mash, next 6 days with protein-free diet and subsequent 6 days with protein-free diets and protein diets containing 10.59% of crude protein supplemented with 0, 0.5, 1.0 and 1.5%, respectively. During experimental feeding period, chicks fed protein-free diets had intaked gradually lower feed and had shown a similar body weight loss though urea contents were increased. When birds fed protein diets, body weight gain and feed intake were not different among birds fed the graded levels of urea although feed conversions were shown a highering tendency along with increasing urea contents. According as supplemented urea were increased, protein efficiency ratio f (PER) and net protein ratio (NPR) were increased in chicks fed protein-free diets, which were shown a lowering trend in chicks fed protein diets. Effect of supplemented urea on the urinary excretion of uric acid were not found in birds fed protein-free diets, while which were increased in birds fed protein diets with the increase of urea contents. Urea addition did not affect the excretion of total creatine in birds fed protein-free or protein diets. Excretion of ammonia was jogjered in order to increasing level of urea in birds fed protein-free diets, but which were not found any particular effect in birds fed protein diets. Also urea excretion were gradually increased with the increasing contents of urea in protein-free and protein diets. Nitrogen balance of birds fed protein-free diets were minus values, which were increased with increasing urea contents in diets. When birds fed protein diets, nitrogen balance and urinary nitrogen excretion was highered and fecal nitrogen excretion were not altered as urea levels of diets increased. Digestibility of urea nitrogen supplemented in protein-free diets were lowered along with increasing contents of urea, but biological value(BV) and net protein utilization(NPU) was found a highering tendency in birds fed protein-free diet containing 1.5% of urea. When birds fed with protein diets, digestibility, BV and NPU of protein were found a highering trend in birds fed protein diets added with 0.5% of urea.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.7
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• pp.1005-1015
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• 2009

Two experiments were carried out to determine the utility of sainfoin hay, a temperate tanniferous forage legume, as a dietary supplement for grass-fed cows. The condensed tannins (CT) of sainfoin might counteract the typical metabolic ammonia load of cows in intensive grazing systems. Furthermore, the physical fibrousness of sainfoin might improve ruminal pH stability. In the preliminary experiment, the eating rate of non-lactating Holstein cows of two tanniferous hays, sainfoin and birdsfoot trefoil, was compared to that of a grass-clover hay after specific periods of time (n = 4). The eating rate of sainfoin was superior to that of the other forages. In the main experiment, designed as a replicated 3${\times}$3 Latin square, six ruminally-cannulated, lactating Red Holstein cows received grass, concentrate and either no supplementation, 3 kg/d of grass hay or 3 kg/d of sainfoin hay (n = 6). Measured intakes of the grass hay and the sainfoin hay were 2.0 and 1.5 kg DM, and two cows entirely refused to eat the sainfoin hay and had to be excluded from data analysis. Grass DMI was similar for cows supplemented with sainfoin hay and cows fed only grass whereas intake of concentrate was higher (p<0.01) for the latter treatment. Continuous measurement of ruminal pH showed that the minimum pH at night tended to be lower (p<0.10) with grass-only feeding compared to sainfoin supplementation, but pH did not decline below the threshold of subacute acidosis for a longer period of time. The slightly higher intake of nitrogen (N) for cows supplemented with sainfoin hay (413 g/d) compared to cows fed only grass (399 g/d) was accompanied by an increased (p<0.05) fecal N excretion and a tendency for an increased (p<0.10) urinary N excretion. Ruminal ammonia concentration, as well as plasma and milk urea, were not affected by sainfoin supplementation. In conclusion, the lack of positive effects typical for CT might be explained either by the limited CT content of this plant species (55 g/kg DM) or the relatively low proportion of sainfoin in the total diet or both. Moreover, due to the unexpected low grass quality, the general ammonia load might have been too low for CT to have an impact.

• Korean Journal of Poultry Science
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• v.24 no.4
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• pp.179-184
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• 1997

Seven-day-old 40 broiler chicks of both sexes were fed the conventional diet containing 0, 0.3, 6 and 0.9％ Quartz porphyry(QP) and water ad libitum until 28 days of age. The excreta water content and nitrogen balance were measured during the experimental period. At the end of the trial, blood samples were collected to measure ammonia, glucose, triglyceride, and total cholesterol concentrations. In addition, intestinal ammonia concentrations were measured. The excreta moisture contents decreased significantly(P < 0.05) as QP levels increased(r= -0.96). Ammonia contents in the intestine tended to increase as QP levels increased, but the ammonia concentration in the blood was not affected by the QP levels. The blood glucose concentration was not changed but blood triglyceride concentration was lower in the 0.3％ QP treatment than the other groups(P < 0.05), Total cholesterol concentration in the blood tended to increase as QP levels increased. The QP 0.3％ treatment showed somewhat higher positive N balance than the other groups. The results of this experiment indicate that the dietary supplementation of QP could offer some benefits to broiler growers.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.8
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• pp.1111-1119
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• 2016

Mao seed is a by-product of the wine and juice industry, which could be used in animal nutrition. The current study was designed to determine the effect of supplementation of mao (Antidesma thwaitesianum Muell. Arg.) seed meal (MOSM) containing condensed tannins (CT) on rumen fermentation, nitrogen (N) utilization and microbial protein synthesis in goats. Four crossbred (Thai Native${\times}$Anglo Nubian) goats with initial body weight (BW) $20{\pm}2kg$ were randomly assigned to a $4{\times}4$ Latin square design. The four dietary treatments were MOSM supplementation at 0%, 0.8%, 1.6%, and 2.4% of total dry matter (DM) intake, respectively. During the experimental periods, all goats were fed a diet containing roughage to concentrate ratio of 60:40 at 3.0% BW/d and pangola grass hay was used as a roughage source. Results showed that supplementation with MOSM did not affect feed intake, nutrient intakes and apparent nutrient digestibility (p>0.05). In addition, ruminal pH and ammonia nitrogen ($NH_3$-N) were not influenced by MOSM supplementation, whilst blood urea nitrogen was decreased quadraticly (p<0.05) in goats supplemented with MOSM at 2.4% of total DM intake. Propionate was increased linearly with MOSM supplementation, whereas acetate and butyrate were remained the same. Moreover, estimated ruminal methane ($CH_4$) was decreased linearly (p<0.05) when goats were fed with MOSM at 1.6% and 2.4% of total DM intake. Numbers of bacteria and protozoa were similar among treatments (p>0.05). There were linear decreases in urinary N (p<0.01) and total N excretion (p<0.01) by MOSM supplementation. Furthermore, N retention was increased linearly (p<0.05) when goats were fed with MOSM supplementation at 1.6% and 2.4% of total DM intake. Microbial protein synthesis were not significantly different among treatments (p>0.05). From the current study, it can be concluded that supplementation of MOSM at 1.6% to 2.4% of total DM intake can be used to modify ruminal fermentation, especially propionate and N utilization in goats, without affecting the nutrient digestibility, microbial populations and microbial protein synthesis.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.1
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• pp.46-54
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• 2014

This study was conducted to investigate the effects of eucalyptus (E. Camaldulensis) crude oils (EuO) supplementation on voluntary feed intake and rumen fermentation characteristics in swamp buffaloes. Four rumen fistulated swamp buffaloes, body weight (BW) of $420{\pm}15.0$ kg, were randomly assigned according to a $2{\times}2$ factorial arrangement in a $4{\times}4$ Latin square design. The dietary treatments were untreated rice straw (RS) without EuO (T1) and with EuO (T2) supplementation, and 3% urea-treated rice straw (UTRS) without EuO (T3) and with EuO (T4) supplementation. The EuO was supplemented at 2 mL/h/d in respective treatment. Experimental animals were kept in individual pens and concentrate mixture was offered at 3 g/kg BW while roughage was fed ad libitum. Total dry matter and roughage intake, and apparent digestibilites of organic matter and neutral detergent fiber were improved (p<0.01) by UTRS. There was no effect of EuO supplementation on feed intake and nutrient digestibility. Ruminal pH and temperature were not (p>0.05) affected by either roughage sources or EuO supplementation. However, buffaloes fed UTRS had higher ruminal ammonia nitrogen and blood urea nitrogen as compared with RS. Total volatile fatty acid and butyrate proportion were similar among treatments, whereas acetate was decreased and propionate molar proportion was increased by EuO supplementation. Feeding UTRS resulted in lower acetate and higher propionate concentration compared to RS. Moreover, supplementation of EuO reduced methane production especially in UTRS treatment. Protozoa populations were reduced by EuO supplementation while fungi zoospores remained the same. Total, amylolytic and cellulolytic bacterial populations were increased (p<0.01) by UTRS; However, EuO supplementation did not affect viable bacteria. Nitrogen intake and in feces were found higher in buffaloes fed UTRS. A positive nitrogen balance (absorption and retention) was in buffaloes fed UTRS. Supplementation of EuO did not affect nitrogen utilization. Both allantoin excretion and absorption and microbial nitrogen supply were increased by UTRS whereas efficiency of microbial protein synthesis was similar in all treatments. Findings of present study suggested that EuO could be used as a feed additive to modify the rumen fermentation in reducing methane production both in RS and UTRS. Feeding UTRS could improve feed intake and efficiency of rumen fermentation in swamp buffaloes. However, more research is warranted to determine the effect of EuO supplementation in production animals.