• Title/Summary/Keyword: tomato gray mold

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Isolation and Characterization of Burkholderia cepacia EB215, an Endophytic Bacterium Showing a Potent Antifungal Activity Against Colletotrichum Species (탄저병균에 길항력이 우수한 식물내생세균 Burkholderia cepacia EB215의 분리 및 특성 규명)

  • Park Ji Hyun;Choi Gyung Ja;Lee Seon-Woo;Jang Kyoung Soo;Lim He Kyoung;Chung Young Ryun;Cho Kwang Yun;Kim Jin-Cheol
    • Microbiology and Biotechnology Letters
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    • v.33 no.1
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    • pp.16-23
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    • 2005
  • In order to develop a new microbial fungicide using endophytic bacteria for the control of anthracnoses occurring on various crops, a total of 260 bacterial strains were isolated from fresh tissues of 5 plant species. After they were cultured in broth medium, their antifungal activities were tested for in vivo antifungal activity against cucumber anthracnose caused by Colletotrichum orbiculare. As the results, liquid cultures of 28 strains showed potent antifungal activities more than $90\%$ against cucumber anthracnose. At 3-fold dilutions of liquid cultures, 18 strains inhibited the development of cucumber anthracnose of more than $70\%$. They were further tested for in vivo antifungal activity against red pepper anthracnose caused by C. coccodes and in vitro antifungal activity against C. acutatum, a fungal agent causing red pepper anthracnose. Among 18 strains, a bacterial strain EB215 isolated from cucumber roots displayed the most potent antifungal activity against Colletotrichum species. It was identified as Burkholderia cepacia based on its physiological and biochemical characteristics, Biolog test and 16S rDNA gene sequence. It also controlled effectively the development of rice blast (Magnaporthe grisea), rice sheath blight (Corticium sasaki), tomato gray mold (Botrytis cinerea), and tomato late blight (Phytophthora infestans). Studies on the characterization of antifungal substances produced by B. cepacia EB215 are in progress.

Control effects of new triazoyl quinolines KSI-4315 and KSI-4317 against barley powdery mildew and wheat leaf rust (신규의 triazoyl quinoline 화합물 KSI-4315와 KSI-4317의 보리 흰가루병과 밀 붉은녹병에 대한 방제효과)

  • Choi, Gyung-Ja;Yon, Gyu-Hwan;Kim, Heung-Tae;Jang, Kyoung-Soo;Kim, Jin-Cheol;Lee, Seon-Woo;Pak, Chwang-Siek;Cho, Kwang-Yun
    • The Korean Journal of Pesticide Science
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    • v.7 no.4
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    • pp.302-309
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    • 2003
  • Disease control activities of 230 triazolyl quinoline derivatives were investigated against six plant diseases such as rice blast, rice sheath blight, tomato gray mold, tomato late blight, wheat leaf rust (WLR) and barley powdery mildew (BPM). New triazolyl quinolines, KSI-4315 and KSI-4317 exhibited a great in vivo control activities against WLR and BPM, and then were selected for further tests such as preventive, curative, systemic, and persistence against WLR and BPM. The KSI-4315 and KSI-4317 contained MeS moiety and MsO moiety in carbon 4-position, respectively. They possessed both preventive activity and curative activity against WLR and BPM. KSI-4317 showed the better control activity than KSI-4315 against BPM, while KSI-4315 represented the better antifungal activity against WLR. Good persistence of KSI-4315 and KSI-4317 were also observed against WLR and BPM. Persistence of KSI-4315 was similar to that of KSI-4317 on WLR, but KSI-4317 was superior to KSI-4315 on BPM in its persistence. Systemic disease control of KSI-4315 and KSI-4317 was investigated by examining translaminar activity from leaf-under-surface to leaf-upper-surface, systemic activities by leaf to leaf movement and the effect of drenching treatment. Systemicities of KSI-4315 and KSI-4317 were not observed in wheat, but KSI-4317 showed more predominant systemicity than KSI-4315 in barley. These results suggest that KSI-4317 would potentially control WLR and BPM in the fields.

Gain Threshold Estimation for Some Pests in Major Crops (주요 작물 몇 가지 병해충에 대한 수익역치 추정)

  • Park, Hong-Hyun;Yeh, Wan-Hae;Park, Hyung-Man
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.63-69
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    • 2007
  • This study was conducted to examine the problems that would arise in our cropping systems when introducing Gain Threshold (GT) which is an important element in determining Economic Injury Level (EIL). Cain Threshold (GT) can be defined as the amount of damage (=yield loss) to justify management, and calculated by dividing the management costs by the market crop price. GTs for some pests in rice, soybean, and greenhouse vegetable crops cultivation were estimated and also compared with those in foreign countries. GTs and percentage of yield loss equivalent to them were estimated to be 4.6-6.1kg/10a/season and 1.0-1.3% for brown planthopper, white-backed planthopper, rice water weevil, and sheath blight, whereas for rice blast in rice cultivation were 12.7kg/10a/season and 2.7%. In soybean cultivation, the values for bean bug were 6.2 kg/10a/season and 3.6%. GTs and percentage of yield loss estimated for melon thrips, whitefly, and downy mildew in cucumber cultivation were 10.0-12.6kg/10a/week, 1.4-1.7%, and the values for two spotted mite and gray mildew in strawberry cultivation were 3.1-3.5kg/10a/week, 1.3-1.5%, and the values for American leaf minor, whitefly, and gray mold in tomato were 8.4-9.7kg/10a/week, 1.7-1.9%. Overall GTs in our cropping systems were so low compared to those in foreign countries, which meant that the low GTs might yield the low EILs. Therefore, we could suggest that prior to direct introduction of GTs calculated from current cultivation systems in developing EILs it is necessary to seriously consider reasonable values of GTs or the yield loss equivalents to them.