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Isolation and Characterization of Burkholderia cepacia EB215, an Endophytic Bacterium Showing a Potent Antifungal Activity Against Colletotrichum Species  

Park Ji Hyun (Biological Function Research Team, Korea Research Institute of Chemical Technology, Division of Applied Life Sciences(BK21 program) and Research Institute of Natural Science, Gyeongsang National University)
Choi Gyung Ja (Biological Function Research Team, Korea Research Institute of Chemical Technology)
Lee Seon-Woo (Biological Function Research Team, Korea Research Institute of Chemical Technology)
Jang Kyoung Soo (Biological Function Research Team, Korea Research Institute of Chemical Technology)
Lim He Kyoung (Biological Function Research Team, Korea Research Institute of Chemical Technology)
Chung Young Ryun (Division of Applied Life Sciences (BK21 Program) and Research Institute of Natural Science, Gyeongsang National University)
Cho Kwang Yun (Biological Function Research Team, Korea Research Institute of Chemical Technology)
Kim Jin-Cheol (Biological Function Research Team, Korea Research Institute of Chemical Technology)
Publication Information
Microbiology and Biotechnology Letters / v.33, no.1, 2005 , pp. 16-23 More about this Journal
Abstract
In order to develop a new microbial fungicide using endophytic bacteria for the control of anthracnoses occurring on various crops, a total of 260 bacterial strains were isolated from fresh tissues of 5 plant species. After they were cultured in broth medium, their antifungal activities were tested for in vivo antifungal activity against cucumber anthracnose caused by Colletotrichum orbiculare. As the results, liquid cultures of 28 strains showed potent antifungal activities more than $90\%$ against cucumber anthracnose. At 3-fold dilutions of liquid cultures, 18 strains inhibited the development of cucumber anthracnose of more than $70\%$. They were further tested for in vivo antifungal activity against red pepper anthracnose caused by C. coccodes and in vitro antifungal activity against C. acutatum, a fungal agent causing red pepper anthracnose. Among 18 strains, a bacterial strain EB215 isolated from cucumber roots displayed the most potent antifungal activity against Colletotrichum species. It was identified as Burkholderia cepacia based on its physiological and biochemical characteristics, Biolog test and 16S rDNA gene sequence. It also controlled effectively the development of rice blast (Magnaporthe grisea), rice sheath blight (Corticium sasaki), tomato gray mold (Botrytis cinerea), and tomato late blight (Phytophthora infestans). Studies on the characterization of antifungal substances produced by B. cepacia EB215 are in progress.
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