• KSBB Journal
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• 제30권3호
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• pp.109-113
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• 2015

Current drying and encapsulation methods for probiotics manufacturing are complicate and cost-burdened processes. The aim of this study was to develop a simple ingredient mixture to make probiotic granules via one-step process, providing not only a cryoprotective effect during freezing and drying but also high survival ratio in gastrointestinal tract. As cryoprotectans, commercially available ingredients including skim milk, monosaccharide (trehalose or glycerin), maltodextrins (with low or high degree of equivalents) were used. Their cryoprotective effect during lyophilization and survival ratios in artificial gastric juice and bile salt were measured against 3 strains of lactic acid bacteria (LAB) (Lactobacillus plantarum, Lb. brevis, and Lactococcus lactis). As results, 3 mixtures with different compositions showed a cryprotective effect on LAB tested and the best compostion was dependant upon LAB; skim milk 10%, trehalose 15%, glycerin 0.5%, and NaCl 1% was for Lb. plantarum and Lc. lactis, and maltodextrin 10% instead of skim milk was for Lb. brevis. In addition, those mixtures showed similar survival effect on LAB tested. These results demonstrate that skim milk or maltodextrins with trehalose, glycerin, and NACl can be effectively used for onestep lyophilization of LAB as an alternative method of encapsulation.

• Journal of Ecology and Environment
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• 제31권4호
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• pp.269-276
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• 2008

In order to understand the role of seed banks for restoration, seed banks in abandoned rice fields in the Gwangneung National Arboretum, central Korea were investigated using the seedling emergence method. The study sites represented three stages: an initial stage dominated by forbs such as Persicaria thunbergii and Juncus effuses var. decipiens, a middle stage dominated by Salix, and a late stage dominated by Quercus aliena and Prunus padus (in nearby riparian forest chosen as a reference stand). DCA ordination arranged the stands according to the number of years since abandonment. CCA ordination identified the dominant environmental variables correlated most closely with Axes 1 and 2 as $Mg^{2+}$ (intraset correlation was 0.827) and $K^+$ (intraset correlation was -0.677), respectively. Species richness and diversity decreased from the initial stage (H'=2.61) to the middle (H'=1.79) and late (H'=0.75) stages. A total of 49 species $(/m^2)$ and 18,620 seedlings $(/m^2)$ emerged out of the seed bank samples. The DCA ordination and similarity analysis detected a large discrepancy between the composition of the actual vegetation and the seed bank. We conclude that the contribution of seed bank to restoration is low. However, seed bank may help the recovery of forbs after disturbance. Some of our results are consistent with the tolerance model of succession whereas others follow the trajectory of the facilitation model. More research on succession will be required to understand the underlying mechanisms.

• BMB Reports
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• 제40권3호
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• pp.426-431
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• 2007

To investigate the possible mechanisms of high-altitude native animals in adapting to high altitude, we cloned hemoglobin alpha-chain (alpha-chain Hb) gene from Pantholops hodgsonii, an animal species that indigenously lives at elevations of 3700-5500 m on the Qinghai-Tibetan plateau. Using reverse transcription polymerase chain reaction (RT-PCR) technique, the alpha-chain Hb gene was amplified from total RNA in the liver of the Pantholops hodgsonii. TA cloning technique was used and the PCR product was cloned into pGEM-T vector. The DNA sequence of the gene was highly homologous with sheep (99.1%), goat (98.6%), cattle (95.6%) and human (86.5%). The alpha-chain Hb gene encoded a 142-amino acid protein that could be identified with the homology of alpha-chain Hb protein in sheep (98%), goat (96%), cattle (91%) and human (87%). However, 18 alternations were detected when compared with the alpha-chain Hb gene in human, and 2 in sheep. Moreover, the alterations of a117 GluAsp and $\alpha$132 AsnSer in important regions were noted in human and sheep, respectively. Phylogenetic analysis suggested that the structure of alpha-chain Hb was highly similar to that in sheep. This study provided essential information for elucidating the possible roles of hemoglobin in adapting to extremely high altitude in Pantholops hodgsonii.

• 식물조직배양학회지
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• 제28권1호
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• pp.53-58
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• 2001

제초제 저항성 오이 (Cucumis sativus L. cv Green angel)를 생산하기 위하여 배발생 현탁배양세포와 binary vector pGA-bar을 지닌 Agrobacterium tumefacians (LBA4404)를 공동배양하였다. 형질전환 벡터의 T-DNA부분에는 kanamycin에 저항성을 나타내는 neomycin phosphotrans ferase (npt II) 유전자와 phosphinothricin (PPT)에 저항성을 나타내는 phosphinothricin acetyltransferase (bar) 유전자를 지니고 있다. 48시간의 공동배양 후 배발생 캘러스는 20mg/L PPT가 함유된 성숙배지에서 배양하였다. 약 200개체의 형질전환 유식물체를 40mg/L PPT가 첨가된 호르몬이 없는 배지에서 생산하였다. 5개의 오이 형질전환 식물체의 염색체에 bar유전자가 도입되어 발현되는 것을 northern blot 분석을 통하여 확인하였다. 형질전환 오이 식물체가 토양에서 성숙되었다. 성숙한 오이 식물체는 PPT가 함유된 상업적 제초제 (Basta)를 일반적인 사용 농도 (3ml/L)처리시에도 저항성을 나타내며 생장하였다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제21권4호
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• pp.315-320
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• 2018

Purpose: This study set out to evaluate the compliance to, and efficacy of oral supplementation, using a 1.5 kcal/mL or 1 kcal/mL sip feed, in children with mild to moderate malnutrition. Methods: This was a parallel, randomized, controlled open-label trial in children aged 3 to 6 years with a weight for height Z (WHZ) score <-1 and ${\geq}-3$, who were randomized to receive a total of 600 kcal/day from either a 1.5 kcal/mL or a 1.0 kcal/mL pediatric sip feed for 28 days. Assessments included daily study product intake, body weight, tolerance and dietary intake from solid food. Results: Of 110 children recruited, 98 ($mean{\pm}standard$ deviation of age $49{\pm}7months$) completed the study. Both sip feeds were well tolerated, with high compliance ($80{\pm}24%$ and $81{\pm}22%$ of prescribed volume in 1.5 kcal/mL and 1.0 kcal/mL groups respectively, p=0.79). Both study groups gained similar weight during the 28 days intervention period ($0.42{\pm}0.40kg$ in 1.5 kcal/mL group vs. $0.49{\pm}0.49kg$ in 1.0 kcal/mL group, p=0.43). There were no significant differences between the groups in weight gain and in the change in WHZ score over the intervention period. Dietary analysis at the end of the study did not show replacement of solid food by the oral nutritional supplements. Conclusion: In children with mild to moderate malnutrition, both 1.5 kcal/mL and 1 kcal/mL pediatric sip feeds had high compliance and were well tolerated, and were equally effective in promoting weight gain in the 28 days study period.

• 생태와환경
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• 제40권4호
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• pp.527-536
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• 2007

In this study, we applied 10-metric health assessment model, based on the Index of Biological Integrity (IBI) during 2006 in the Changwon Stream, which is located in the Changwon city, Gyeongnam province, S. Korea, and then compared with water quality data. The Index of Biological Integrity (IBI) in the Changwon Stream varied from 18 to 38 in the watershed depending on the sampling location and averaged 30.3 (n=6) during the study. Analysis of tolerance guilds showed that the proportion of sensitive species was 13%, but tolerant and intermediate species were 34% and 53%, respectively. Qualitative Habitat Evaluation Index (QHEI) averaged 43.3 (range: 65-104, n=6) indicating non-supporting condition, based on the criteria of U.S. EPA (1993). Values of QHEI showed a typical longitudinal decreases from the headwater reach to the downstream location, except for Site 1 with a low QHEI value by artificial habitat by concrete construction. Minimum QHEI was found in Site 4 where fish diversity was minimal. Conductivity increased continuously along the gradients and especially showed abrupt increases in the downstream sites along with turbidity. Stream ecosystem health of IBI matched to the values of QHEI except for S6. Low IBI values in the sites 4 and 5 was considered to be a result of combined effects of chemical pollutions and habitat degradations. Our results support the hypotheses of Plafkin et ai. (1989) that physical habitat quality directly influences the trophic structure and species richness, and is closely associated with IBI values.

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7401-7405
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• 2014

Background: Postoperative chemoradiotherapy is accepted as standard treatment for stage IB-IV, M0 gastric cancer. Radiotherapy (RT) planning of gastric cancer is important because of the low radiation tolerance of surrounding critical organs. The purpose of this study was to compare the dosimetric aspects of 2-dimensional (2D) and 3-dimensional (3D) treatment plans, with the twin aims of evaluating the adequacy of 2D planning fields on coverage of planning target volume (PTV) and 3D conformal plans for both covering PTV and reducing the normal tissue doses. Materials and Methods: Thirty-six patients with stage II-IV gastric adenocarcinoma were treated with adjuvant chemoradiotherapy using 3DRT. For each patient, a second 2D treatment plan was generated. The two techniques were compared for target volume coverage and dose to normal tissues using dose volume histogram (DVH) analysis. Results: 3DRT provides more adequate coverage of the target volume. Comparative DVHs for the left kidney and spinal cord demonstrate lower radiation doses with the 3D technique. Conclusions: 3DRT produced better dose distributions and reduced radiation doses to left kidney and spinal cord compared to the 2D technique. For this reason it can be predicted that 3DRT will result in better tumor control and less normal tissue complications.

• 전자공학회논문지
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• 제50권6호
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• pp.85-90
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• 2013

본 논문에서는 본 연구실에서 제안된 Dummy Gate Assisted MOSFET을 이용하여 6bit SAR (Successive Approximation Register) ADC를 설계하였으며 이에 대한 대조군으로 Conventional MOSFET으로 동일한 회로를 설계하여 두 회로의 Co-60 Gamma Ray에 의한 누적방사선 영향을 비교 분석해 보았다. 설계된 SAR ADC는 Binary Capacitor DAC과 Dynamic Latch 형태의 Comparator 그리고 Logic으로 구성이 되었으며, 0.35um standard CMOS공정으로 제작되었다. 방사선 조사 후 Conventional MOSFET을 이용한 ADC는 정상동작하지 못하였지만, Dummy Gate Assisted MOSFET을 사용한 ADC는 방사선 조사 후 DNL은 0.7LSB에서 2.0LSB, INL은 1.8LSB에서 3.2LSB로 다소 증가하였으나 정상적인 A/D 변환이 가능하다는 것을 확인하였다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2007년도 추계학술대회 논문집
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• pp.1610-1615
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• 2007

This paper presents a characteristics of linear induction motor considering airgap variation for railway transit in order to achieve high performance of the vehicle. The operating principle of a LIM(Linear induction motor) is identical to a rotary induction motor. Space-time variant magnetic fields are generated by the primary part across the airgap and induce the electro-motive force(EMF) in the secondary part, a conducting sheet. This EMF generates the eddy currents, which interact with the airgap flux and so produce the thrust force known as Loren's force. Even though the operating principal is exactly same as a rotary motor, the linear motor has a finite length of the primary or secondary parts and it causes static and dynamic end-effect which is the discontinuous airgap flux phenomenon. This end-effect causes the deterioration of the system performance, especially in high-speed operation. Another problem is that construction tolerance restricts the minimum airgap in order to prevent a collision between the primary part and the secondary reaction plate. More over, as the airgap length is getting smaller, the attraction force between the primary part and secondary parts is getting larger dramatically and the attraction force would be another friction against propulsion. Therefore, it is necessary to figure out the characteristics of linear induction motor considering airgap variation in order to achieve high performance of the vehicle. The dynamic model of LIM taking into account end-effects is derived. Then the modified mechanical load equation considering the effect of the attraction and thrust force according to the airgap variation is analyzed. The simulation results are presented to show the effect of the LIM according to the airgap variation.

• 한국광학회지
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• 제15권3호
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• pp.241-247
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• 2004

고해상도 인공위성용 망원경의 조립 및 평가를 위해서는 평행빔 만들어주는 시준장치가 꼭 필요하다. 전통적으로 큰 직경의 시준장치로는 비축포물면 거울을 많이 사용하여 왔다. 하지만, 평가대상망원경의 초점거리가 긴 경우 비축포물면은 보다 긴 초점거리를 가져야 하고 공기의 유동효과를 효과적으로 제어하지 못할 경우, 정확한 평가가 이루어지기 어려운 단점이 있다. 이에 비해 Cassegrain 형태 시준장치는 빔을 꺾어 사용하므로, 초점거리에 비해 적은 공간을 차지하고 공기의 유동효과도 상당히 줄일 수 있는 장점이 있다. 따라서, 본 논문에서는 직경 300 mm 인공위성 망원경을 측정하기 위해 직경 450 mm 의 Cassegrain 형태 시준장치를 설계하고 제작한 과정을 설명한다. 주경 및 부경을 제작한 후 시준장치를 구성한 결과 최종 파면수차는 0.07λ(λ=633 nm)로 diffraction limited 성능을 보였다.