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http://dx.doi.org/10.7841/ksbbj.2015.30.3.109

Analysis of Ingredient Mixtures for Cryoprotection and Gastrointestinal Stability of Probiotics  

Jeong, Eun Ji (TOBICO Inc.)
Moon, Dae Won (TOBICO Inc.)
Oh, Joon Suk (TOBICO Inc.)
Moon, Jin Seok (Department of Food Science and Biotechnology, Chungbuk National University)
Kim, Kwang Yup (Department of Food Science and Biotechnology, Chungbuk National University)
Choi, Hye Sun (Department of Agrofood Resources, NAAS, RDA)
Han, Nam Soo (Department of Food Science and Biotechnology, Chungbuk National University)
Publication Information
KSBB Journal / v.30, no.3, 2015 , pp. 109-113 More about this Journal
Abstract
Current drying and encapsulation methods for probiotics manufacturing are complicate and cost-burdened processes. The aim of this study was to develop a simple ingredient mixture to make probiotic granules via one-step process, providing not only a cryoprotective effect during freezing and drying but also high survival ratio in gastrointestinal tract. As cryoprotectans, commercially available ingredients including skim milk, monosaccharide (trehalose or glycerin), maltodextrins (with low or high degree of equivalents) were used. Their cryoprotective effect during lyophilization and survival ratios in artificial gastric juice and bile salt were measured against 3 strains of lactic acid bacteria (LAB) (Lactobacillus plantarum, Lb. brevis, and Lactococcus lactis). As results, 3 mixtures with different compositions showed a cryprotective effect on LAB tested and the best compostion was dependant upon LAB; skim milk 10%, trehalose 15%, glycerin 0.5%, and NaCl 1% was for Lb. plantarum and Lc. lactis, and maltodextrin 10% instead of skim milk was for Lb. brevis. In addition, those mixtures showed similar survival effect on LAB tested. These results demonstrate that skim milk or maltodextrins with trehalose, glycerin, and NACl can be effectively used for onestep lyophilization of LAB as an alternative method of encapsulation.
Keywords
Probiotics; Lactic acid bacteria; Acid tolerance; Bile resistance; Cryoprotectant agents; freeze drying;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 The Korea Food and Drug Administration, Available from: http://www.mfds.go.kr/index.do?mid=675&seq=24741&cmd=v. (2014).
2 The Korea Food and Drug Administration, Available from: http://www.foodnara.go.kr/hfoodi. (2014).
3 FULLER, Ray (ed.). (2012) Probiotics: the scientific basis. pp. 111-144. Springer Science & Business Media, London.
4 Heckley, R. J. (1978) Preservation of microorganisms. Adv. Appl. Microbiol. 24: 15-28.
5 Morich, T. (1973) Lactic acid bacteria in animal industry. Japanese J. Zootech. Sci. 44: 535-553.
6 Deman, J. C., M. Rogosa, and M. E. Shape (1960) A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23: 130.   DOI
7 Heckley, R. J. (1961) Preservation of bacteria by lyophilization. Adv. Appl. Microbiol. 3: 1-28.   DOI
8 Yun, H. J., Y. J. Lee, S. H. Yeo, H. Y. Park, H. D. Park, S. Y. Baek (2013) Isolation and characterization of exopolysaccharide producing lactic acid bacteria from Korean soy sauce and soybean paste. Korean J. Microbiol. Biotechnol. 41: 190-197.   DOI
9 Hood, S. K. and E. A. Zittola (1998) Effect of low pH on the ability of Lactobacillus acidophilus to survive and adhere to human intestinal cells. J. Food Sci. 53: 1514-1516.
10 Kwak, H. S. and A. Feucht (2013) Microencapsulation of lactic acid bacteria. Korean J. Food Sci. An. 33: 229-238.   DOI
11 Burgain, J., C. Gaiani, M. Linder, and J. Scher (2011) Encapsulation of probiotic living cells: From laboratory scale to industrial applications. J. Food Eng. 104: 467-483.   DOI   ScienceOn
12 Heidebach, T., P. Forst, and U. Kulozik (2010) Influence of caseinbased microencapsulation on freeze-drying and storage of probiotic cells. J. Food Eng. 98: 309-316.   DOI
13 Cook, M. T., G. Tzortzis, D. Charalampopoulos, and V. V. Khutoryanskiy (2014) Microencapsulation of a synbiotic into PLGA/alginate multiparticulate gels. Int. J. Pharm. 466: 400-408.   DOI
14 Kobayashi, Y., K. Tohyamat, and T. Terashima (1974) Biological characteristics of Lactobacillus Tolerance of the multiple antibiotic resistance strain, L. casei PSR3002, to artificial digestive fluids. Nihon Saikingaku Zasshi 29: 691-697.   DOI
15 Shin, M. S., J. J. Lee, S. H. Na, H. S. Bae, C. S. Huh, and Y. J. Baek (1998) Characteristics of Bifidobacterium spp. Isolated from korean faces for probiotics. Korean J. Dairy Sci. 20: 273-282.
16 Paik, H. D., M. Y. Jung, W. S. Kim, and K. T. Kim (2002) Characterization of bacillus polyfermenticus SCD for oral bacteriotherapy of gastrointestinal disorders. Korean J. Food Sci. Technol. 34: 73-78.
17 Cell biotech Co., Ltd. (2011) Lactic acid bacteria having multi-coting layers and preparing method thereof. Korea Patent, 10-2011-0093074.