• The Korean Journal of Physiology and Pharmacology
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• v.12 no.5
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• pp.275-280
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• 2008

A brief ischemic insult induces significant protection against subsequent massive ischemic events. The molecular mechanisms known as preconditioning (PC)-induced ischemic tolerance are not completely understood. We investigated whether kinetic changes of cyclooxygenase (COX)-2 during reperfusion time-periods after PC were related to ischemic tolerance. Rats were given PC by occlusion of middle cerebral artery (MCAO) for 10 min and sacrificed after the indicated time-periods of reperfusion (1, 2, 4, 8, 12, 18 or 24 h). In PC-treated rats, focal ischemia was induced by occlusion of MCA for 24 h and brain infarct volume was then studied to determine whether different reperfusion time influenced the damage. We report that the most significant protection against focal ischemia was obtained in rats with 8 h reperfusion after PC. Administration of indomethacin (10 mg/kg, oral) or rofecoxib (5 mg/kg, oral) 48 h prior to PC counteracted the effect of PC. Immunohistochemical analysis showed that COX-2 and HO-l protein were induced in PC-treated rat brain, which was significantly inhibited by rofecoxib. Taken together, we concluded that the kinetic changes of COX-2 expression during the reperfusion period after PC might be partly responsible for ischemic tolerance.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.26-32
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• 2014

Recent genome annotation revealed that Populus trichocarpa contains 81 glutathione S-transferase (GST) genes. GST genes play important and varying roles in plants, including conferring tolerance to various abiotic stresses. Little information is available on the relationship - if any - between drought/salt stresses and GSTs in woody plants. In this study, we screened the PatgGST genes in hybrid poplar (Populus alba ${\times}$ Populus tremula var. glandulosa) that were predicted to confer drought tolerance based on our expression analysis of all members of the poplar GST superfamily following exposure to salt (NaCl) and drought (PEG) stresses, respectively. Exposure to the salt stress resulted in the induction of eight PatgGST genes and down-regulation of one PatgGST gene, and the level of induction/repression was different in leaf and stem tissues. In contrast, 16 PatgGST genes were induced following exposure to the drought (PEG) stress, and two were down-regulated. Taken together, we identified seven PatgGSTs (PatgGSTU15, PatgGSTU18, PatgGSTU22, PatgGSTU27, PatgGSTU46, PatgGSTU51 and PatgGSTU52) as putative drought tolerance genes based on their induction by both salt and drought stresses.

• Molecules and Cells
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• v.27 no.4
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• pp.449-458
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• 2009

The OsAsr1 cDNA clone was isolated from a cDNA library prepared from developing seed coats of rice (Oryza sativa L.). Low-temperature stress increased mRNA levels of OsAsr1 in both vegetative and reproductive organs. In situ analysis showed that OsAsr1 transcript was preferentially accumulated in the leaf mesophyll tissues and parenchyma cells of the palea and lemma. For transgenic rice plants that over-expressed full-length OsAsr1 cDNA in the sense orientation, the Fv/Fm values for photosynthetic efficiency were about 2-fold higher than those of wild type-segregating plants after a 24-h cold treatment. Seedlings exposed to prolonged low temperatures were more tolerant of cold stress, as demonstrated during wilting and regrowth tests. Interestingly, OsAsr1 was highly expressed in transgenic rice plants expressing the C-repeat/dehyhdration responsive element binding factor 1 (CBF1), suggesting the regulation of OsAsr1 by CBF1. Taken together, we suggest that OsAsr1 gene play an important role during temperature stress, and that this gene can be used for generating plants with enhanced cold tolerance.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.3
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• pp.223-230
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• 2017

Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) is an upstream signaling molecule that has been shown to induce stress tolerance in plants. In this study, the AtNDPK2 gene, under the control of a stress-inducible SWPA2 promoter, was introduced into the genome of tall fescue (Festuca arundinacea Schreb.) plants. The induction of the transgene expression mediated by methyl viologen (MV) and NaCl treatments were confirmed by RT-PCR and northern blot analysis, respectively. Under salt stress treatment, the transgenic tall fescue plants (SN) exhibited lower level of $H_2O_2$ and lipid peroxidation accumulations than the non-transgenic (NT) plants. The transgenic tall fescue plants also showed higher level of NDPK enzyme activity compared to NT plants. The SN plants were survived at 300 mM NaCl treatment, whereas the NT plants were severely affected. These results indicate that stress-inducible overexpression of AtNDPK2 might efficiently confer the salt stress tolerance in tall fescue plants.

• Design & Manufacturing
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• v.15 no.2
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• pp.42-48
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• 2021

In this paper, when manufacturing large/multi-mold parts (more than 30 core parts),A mold manufacturing (tolerance) management system was established through design-processing linkage.The mold manufacturing (tolerance) management system is a design-based measurement shape/measurement position determination system, M/C processing-linked measurement drive system,It is composed of four parts: CAD-linked measurement result analysis system and manager mold part quality management system.In addition, the constructed system was applied to the field and the effect of system construction was evaluated by comparing it with the existing process.As a result of the evaluation, the measurement precision is within 0.02mm, and the time it takes to measure after the end of processing is shorter than that of the existing process.(12 hours → 2 hours) It was shortened to 16.7%.In addition, it was confirmed that the time required for reprocessing after measurement was reduced by 25% (4 hours → 1 hour) compared to the existing process.

• Journal of The Korean Association For Science Education
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• v.29 no.4
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• pp.414-422
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• 2009

In this study, we investigated the influences of students' motivational characteristics on the processes of learning density concept using a discrepant event. The participants were 642 seventh graders from two middle schools. Tests of failure tolerance, self-efficacy and mastery/performance goal orientation were administered as pretests. A preconception test was also administered. The intervention was the students' individual study of the density concept with a worksheet that was designed to incorporate the major steps of conceptual change learning. The tests of attention, effort and conceptual understanding were administered as post-tests. The responses of 203 students who had been found to possess the target misconception were analyzed. The results of a path analysis revealed that students' motivational characteristics variables did not influence cognitive conflict. Failure tolerance and mastery goal orientation, however, influenced conceptual understanding via situational interest, attention and effort. Self-efficacy influenced conceptual understanding via effort. Performance goal orientation negatively influenced conceptual understanding via attention and effort. Cognitive conflict influenced conceptual understanding directly as well as indirectly via situational interest.

• Journal of Korean Society for Quality Management
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• v.25 no.2
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• pp.140-153
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• 1997

As product quality is maily determined in the product design and process design step, systematic design should be performed through parameter design and tolerance design. Therefore, we introduced analysis of variance and regression analysis as a statistical method which determine optimal levels of affective design factors to product characteristics, then we compared that process and result. In analysis of variance, variation of quality characteristics arises from noise factors, so the optimal levels of design factors are selected to minimize the effect of noise factors. In regression analysis, variation of quality characteristics aries from variation of each own design factors. As a method to reduce variation of these quality characteristics, sensitivity analysis was performed about each design factors. Through this sensitivity analysis, we represented process to calculate the interaction term of the factors.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2004.10a
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• pp.47-50
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• 2004

It is very important that the analysis of postbuckling characteristics for stiffened composite structure. Damage tolerance design concept has been imported in many aerospace structures design to increase the structural efficiency and material failure is an inevitable process in postbuckling behavior. These design concepts are very efficient but consume very much calculation time in analytical process. The proper analysis method for reducing calculation time was researched and the total analysis time was reduced. A selection of proper element, the large load increments in linear response region, and the termination of analysis for unnecessary region were imported in the analysis and about $70\%$ time reduction was achieved with keeping a high accuracy of results.

• Journal of the Society of Naval Architects of Korea
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• v.44 no.4
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• pp.425-438
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• 2007

In this paper, unsettled technical controversies concerning about fatigue strength analysis for FPSO, one of the representative floaters, associated with welding types, screening methods, fabrication tolerances, corrosion margins and Morison loads are described based on yard practices. Basic theory for stochastic fatigue analysis is introduced as detail as possible. In order to resolve large parts of the controversies, a new fully stochastic fatigue analysis program for FPSO is developed.

• Mass Spectrometry Letters
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• v.15 no.1
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• pp.26-39
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• 2024

Gold nanostructures (Au NSs) are useful and interesting matrices for mass spectrometric analysis of various biomolecules based on organic matrix-free laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF-MS). Au NSs provide high efficiency and versatility in LDI-TOF-MS analysis based on their well-established synthesis and surface functionalization, large surface area, high laser absorption capacity, and photothermal conversion efficiency. Therefore, Au NSs based LDI-TOF-MS can be a facile, functional, and efficient analytical method for important small biomolecules owing to its simple preparation, rapid analysis, salt-tolerance, signal reproducibility, and quantitative analysis. This review chronologically summarizes the important advance of Au NSs-based LDI-TOF-MS platforms in terms of in-depth mechanism, signal enhancement, quantitative analysis, and disease diagnosis.