• Proceedings of the Ginseng society Conference
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• 1984.09a
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• pp.1-11
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• 1984

Ginseng cotyledon calli were cultured on 1/2MS media supplemented with combination of various growth regulators to induce more embryoids and plantlets in a short period. And tissues of ginseng root and calli were also incubated under various factors or conditions to establish methods for the isolation of viable protoplasts in Panax ginseng C.A. Meyer. The calli derived from cotyledon produced numerous embryoids in 1/2MS media containing 0.5mg/$\ell$ 2,4-D and 0.5mg/$\ell$ kinetin after 2 months' culture. But only shoot formation was less frequent. Further development of these embryoids occurred on 1/2MS medium supplemented with the same concentration of BA and GA. Viable protoplasts were isolated from the root tissue and callus of ginseng. The specific conditions for the isolation of viable protoplasts were required of ginseng materials, root tissue and callus, being processed. For the production of viable protoplasts from 1-year old ginseng root tissue, an enzyme mixture of $2\%$ cellulase 'Ono-zuka' and $0.5\%$ macerozyme, an enzyme solution pH of 5.2 to 5.8, a 7- to 8- hour incubation period at $28{\pm}1^{\circ}C$, and 0.9M mannitol as osmoticum in the cell enzyme mixture were optimum, while the treatments with an enzyme mixture of $2\%$ cellulase 'Onozuka', $2\%$ macerozyme and $1\%$ driselase, and 25-hour incubation period at $28{\pm}1^{\circ}C$, were more efficient for the production of viable protoplasts from ginseng callus.

• Journal of Sericultural and Entomological Science
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• v.16 no.1
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• pp.67-75
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• 1974

The studies are to know how much cocoon crops is damaged by the stained leaf with nicotine produced from the tobacco field cultivated in mulching system in spring season and by residual nicotine in autumn season. Furthermore, the new knowledges are to make both industries keep up with their development. In spring season mulberry Held is located higher on the West-North of tobacco held below 20 degrees of slope and with 36 per cent of East-South wind and 18 per cent of South wind blowing from tobacco fold to the mulberry fold. In addition, silkworm larvae are fed with the mulberry leaf produced in the different plots placing by the different distances, l0m, 25m, 50m, 80m, and loom far from the tobacco Held as a control and it is also considered that narcotic larvae including the dead larvae are not observed. On the other hand, it is noted that better leaf quality and abundant growth of mulberry tree is produced from the mulberry fold closer to the tobacco field and with a low slope. 1) Maximum weight of larval body at the 5th stage is damaged by the stained leaf with the nicotine up to 25m far from the tobacco held. 2) The larvae fed with the mulberry leaf in mulberry Held up to 25m far from the tobacco fold produce small number of the fresh cocoons per 1 liter. 3) Low single cocoon weight and low cocoon shell weight are produced by the poison damaged larvae fed with the mulberry. leaf up to 25m far from the tobacco field and weight of cocoon shell is damaged higher than the single cocoon weight. It is resulted in low percentage of cocoon shell. 4) Cocoon yield including the double cocoon from 10,000 larvae is decreased by the larvae fed with the stained leaf in the mulberry fold up to 25m far from the tobacco fold and 19 per cent of cocoon yield is decreased with 2.4kg of cocoon yield in l0m plot and with 2.5kg of cocoon yield in 25m plot at the first season and at the 2nd season with 1.8kg o( cocoon yield in l0m plot and with 11.5kg of cocoon yield in 25m plot, 11 per cent and 9 per cent of cocoon yield including double cocoon from 10,000 larvae is decreased, as compared with the control, respectively. With these results, it is observed that nicotine damage is occurred to the silkworm larvae if the larvae are fed with the leaf in the mulberry Held within 25m-50m far from the tobacco field.

• Current Research on Agriculture and Life Sciences
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• v.1
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• pp.35-40
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• 1983

This study was conducted to obtain basic informations for growth regulators on occurrence of sucker in tobacco plants (Nicotiana tabacum L.). Varieties used were "hicks" and "kusaga mammoth" and growth regulators such as MH(maleic hydrazide), GA(Gibberellic acid) and BA(Benzyl adenine) were used. Immediately after topping, an application of maleic hydrazide at 900g a.i./ha completely inhibited sucker development, but sucker were developed as the rates of MH decreased, in both varieties. In nontopped tobacco plants, the similar trend as in the topped plant was observed except for no sucker development in the untreated control. Any combination of GA and BA under presence of MH had no effects on sucker development in the topped tobacco plants. However, in the nontopped plants, sucker were observed when the combined ratio of BA and GA was 10 to 1 under the presence of MH standard level. The highest no. of sucker was obtained when combined BA $10^{-5}M$ with GA $10^{-6}M$ under the presence of MH, showing higher response of hicks than that of kusaga mammoth. A single application of GA and BA in the topped plants markedly increased sucker number as GA concentrations increased showing varietal difference. GA $10^{-4}M$ increased sucker number as high as as 42% for hicks, but inhibitory effect on kusaga mammoth in comparision with the untreated control, showing very effective on hicks. BA showed the similar effect like GA. Combinations of GA and BA showed antagonistic effect on sucker development. The length of sucker was markedly promoted as the GA rates increased, and the promotive effect of sucker length by GA was not nullified by the addition of BA. But combination treatment of GA and BA mostly resulted in less dry weight than the untreated control, indicating that sucker developed from the combined treatments of GA and BA were not normal and kusaga mammoth was more affected by them.

• Journal of Ginseng Research
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• v.40 no.4
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• pp.453-461
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• 2016

Background: This study aims to describe the characterization of Paenibacillus polymyxa GBR-1 (GBR-1) with respect to its positive and negative effects on plants. Methods: The morphological characteristics of GBR-1 were identified with microscopy, and subjected to Biolog analysis for identification. Bacterial population and media optimization were determined by a growth curve. The potential for GBR-1 as a growth promoting agent, to have antagonistic activity, and to have hydrolytic activity at different temperatures was assessed. The coinoculation of GBR-1 with other microorganisms and its pathogenicity on various stored plants, including ginseng, were assessed. Results: Colony morphology, endospore-bearing cells, and cell division of GBR-1 were identified by microscopy; identification was performed by utilizing the Biolog system, gas chromatography of fatty acid methyl esters (GC-FAME). GBR-1 showed the strongest antagonistic activity against fungal and bacterial pathogens. GBR-1 cell numbers were relatively higher when the cells were cultured in brain heart infusion (BHI) medium when compared with other media. Furthermore, the starch-hydrolytic activity was influenced by GBR-1 at higher temperature compared to low temperatures. GBR-1 was pathogenic to some of the storage plants. Coinoculation of GBR-1 with other pathogens causes differences in rotting on ginseng roots. A significant growth promotion was observed in tobacco seedlings treated with GBR-1 suspensions under in vitro conditions, suggesting that its volatile organic compounds (VOCs) might play a role in growth promotion. Conclusion: The results of this study indicate that GBR-1 has both positive and negative effects on ginseng root and other stored plants as a potential biocontrol agent and eliciting in vitro growth promotion.

• KSBB Journal
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• v.22 no.5
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• pp.313-317
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• 2007

To enhance the growth of cryopreserved cells of transgenic Nicotiana tabacum, Pluronic F-68 was supplemented in a recovery medium during post-thaw period. As cryoprotective agents, 1 M sucrose, 0.5 M glycerol and 0.5 M dimethyl sulfoxide (DMSO) were added before freezing steps. The post-thaw growth of the cells was improved with Pluronic F-68, ranged from 0.1 to 10 g/L. The interactions of Pluronic F-68 with the cells were confirmed by the changes of hydrophobicity or permeability of the cells. Pluronic F-68 did not show any effect on the activity of $\beta$-glucuronidase (GUS) in all treatments. Therefore, the addition of Pluronic F-68 in a recovery medium was found to be beneficial to enhance the post-thaw growth of cryopreserved transgenic tobacco cells without affecting the production of recombinant protein.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.6
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• pp.497-503
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• 1990

A field experiment was conducted to find out the effects of nitrogen and potassium rates on the growing characters, yield and quality of burley tobacco (1988 : Burley 21, 1989: Burley 21 and KB101) during successive two years. The effect of added nitrogen fertilizer on the growth characters at topping stage was different according to growing years. Potassium rate had no effect on the growth characters. The effects of nitrogen and potassium rates on the nitrogen conteut of leaf during growth stage were great, while the effects on the potassium content were not large, relatively. As compared with Burley 21, KB101 showed high yield, and the productivity in nitrogen-reduced condition was high. The yield and quality were increased when nitrogen applied to 22.75kg/l0a, but the yield was not increased and the quality was decreased when nitrogen applied above 22.75kg/l0a. Neither potassium rate nor potassium/nitrogen application ratio had effects on the yield and quality, while the quality was somewhat decreased when potassium/nitrogen ratio was small. When the nitrogen fertilizer being applied above 22.75kg/l0a, there were no advantageous effects on the yield and quality, It is sufficient that the potassium application is about 35.0kg/l0a, and the scheme of reducing of potassium rate may be considerable.

• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.197-203
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• 1999

The albino plants of tobacco (Nicotiana tobacum L. cv. BY-4) were isolated from seed populations that were induced by heavy-ion ($^{14}N$) beam irradiation to proembryo and the in vitro growth and differentiation have been characterized. The in vitro cultured albino plants showed significant reduction of chlorophyll content and possessed larger number of stomata on both upper and lower epidermis than that of wild-type plants. Stem growth of the mutants remained dwarfed, however, the internode recovered its normal length after GA$_3$ treatment (10.0mg/L) on the MS medium containing sucrose under continuous light. When explants of leaf blades of albino plants were cultured, multiple shoots formed directly on MS medium containing 1.0mg/L of BAP or kinetin and a large number of calli were induced on the MS medium containing 1.0mg/L NAA or 1.0 mg/L 2,4-D. The albino calli regenerated multiple albino plantlets in the MS medium containing 0.1mg/L NAA + 1.0 mg/L BAP. No significant differences between the wild-type and albino plants were detected in the multiple shoot induction, callus formation from the explants and the plantlets regeneration from calli. In addition, albino plants have a similar organogenesis Pattern to that of the wild-type in the media with different combinations of NAA (0 to 5.0mg/L) and BAP (0 to 5.0mg/L) treatment. These results indicate that the albino mutant has the same normal regeneration ability as that of wild-type, although the mutant has lost functions in photosynthesis, such as pigmentation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.1
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• pp.22-32
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• 1997

This experiment was conducted to obtain information for the cultivation of Korean valerian(Valeriana lauriei var. dasycarpa Hara) which will be useful for medicinal and aromatic resources. The effect of different temperature conditions, light intensities and soil water conditions on growth, yield and component of essential oil of V. fauriei were measured at the Dankook University, Cheonan, and a study on the shading treatment was at Umsung, Chungchongbukdo, and Jinbu, Kangwondo, in 1995. V. laudei was planted at five different temperature conditions, 10, 15, 20, 25 and 3$0^{\circ}C$, eight light intensity conditions, 1, 000, 2, 500, 5, 000, 20, 000, 30, 000, 40, 000, 50, 000 and 60, 000lux, six soil water contents, 30, 45, 55, 70, 80 and 90％ of the saturated soil, during growth stage. Shading treatment was three conditions, 0, 25 and 50％, during the daytime in field conditions. Photosynthesis had a highly significant relationship with temperature conditions in a quadratic regression model, from which the temperature for the plant growth was estimated to be 17.7$^{\circ}C$. A highly significant quadratic regression was noted between temperature and leaf width or root weight of V. fauriei. It was estimated from the regression equation that the optimum temperature for root growth was 20.3$^{\circ}C$. The content of essential oil and extract rate of root was the highest in the 15~2$0^{\circ}C$. Photosynthesis also was significantly affected by light intensity in a quadratic regression model, from which the optimum light intensity for the growth was estimated to be 40, 000lux. Root yield was more produced in Jinbu than that of in Umsung. The root yield was increased by the shading treatment in Umsung, whereas it was decreased by the shading treatment in Jinbu. The content of essential oil was not affected by the shading treatment of plants during the cultivation, while the compositions of components of essential oil were related to the growing locations. As soil water content was higher, the growth and content of root extract were increased. The optimum soil moisture for the growth of V. fauriei was 80~90％ of the saturated soil. In summary, the results indicated that the growth, yield and component of essential oil in V. fauriei were affected by environmental factors as well as soil moisture.

• Journal of Ginseng Research
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• v.22 no.4
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• pp.244-251
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• 1998

KG101 was developed by the Korea Ginseng & Tobacco Research Institute. We reported about yield trials and local adaptability test. Growth characteristics and red ginseng quality of KG101 in local performance test can be summarized as follows. Aerial parts growth, stem and leaf, was similar about KG101 and Jakyungjong, 4-year old and 6-year old ginseng. Emergence rate of KG101 was higher than Jakyungjong in the 3 location of local performance test. Taproot diameter of KG101 was smaller than Jakyungjong and taproot shape of KG101 had fitted characteristics, radish type and human body type, to manufactured red ginseng. Raw ginseng grade, 1st and 2nd of KG101 was higher than Jakyungjong's. Chun, Jeesam ratio to manufacture of 2nd grade of raw ginseng of KG101 was 25%, Jakyungjong was 6-13% in 3 location of local performance test. Distribution of red ginseng weight of Jakyungiong was heavier than KG101 in 2 location, Kongju and Jungup, but KG101 was heavier than Jakyungjong in Ichon. Red ginseng quality of KG101 was better than Jakyungiong. Main degrade factor of red ginseng quality was inside cavity and inside white, was not different lines and location.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.81-83
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• 1995

This study was conducted to know the effect of soil moisture content on growth of aerial parts and root of Valeriana fauriei var. dayscarpa HARA in pot condition. The soil moisture contents were 30, 45, 55, 70, 80 and 90% of maximum water requirement. The result obtained are as follows; 1. Width and length of leaves in valerian were slightly increased with increase the soil moisture content, however, length of petiole and root were increased with soil moisture content up to 80%. 2. It showed the positive correlations between moisture content and root weight and extract content in root. 3. Optimum soil moisture content was 80 to 90% of maximum water requirement.