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Effect of Pluronic F-68 on the Post-thaw Growth of Cryopreserved Transgenic Nicotiana tabacum Cells  

Cheon, Su-Hwan (Department of Biological Engineering, Inha University)
Lee, Kyoung-Hoon (Department of Biological Engineering, Inha University)
Kwon, Jun-Young (Department of Biological Engineering, Inha University)
Ryu, Hyun-Nam (Department of Biological Engineering, Inha University)
Kim, Dong-Il (Department of Biological Engineering, Inha University)
Publication Information
KSBB Journal / v.22, no.5, 2007 , pp. 313-317 More about this Journal
Abstract
To enhance the growth of cryopreserved cells of transgenic Nicotiana tabacum, Pluronic F-68 was supplemented in a recovery medium during post-thaw period. As cryoprotective agents, 1 M sucrose, 0.5 M glycerol and 0.5 M dimethyl sulfoxide (DMSO) were added before freezing steps. The post-thaw growth of the cells was improved with Pluronic F-68, ranged from 0.1 to 10 g/L. The interactions of Pluronic F-68 with the cells were confirmed by the changes of hydrophobicity or permeability of the cells. Pluronic F-68 did not show any effect on the activity of $\beta$-glucuronidase (GUS) in all treatments. Therefore, the addition of Pluronic F-68 in a recovery medium was found to be beneficial to enhance the post-thaw growth of cryopreserved transgenic tobacco cells without affecting the production of recombinant protein.
Keywords
Pluronic F-68; cryopreservation; Nicotiana tabacum; post-thaw growth; transgenic plant cell cultures;
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