• 한국연초학회지
• /
• 제17권1호
• /
• pp.79-97
• /
• 1995

On the basis of the literature, which is at present still scientific information improvable, the results of research done so far into the occurrence and analytical methodology of major markers of environmental tobacco smoke(ETS) have been summarized. Key areas addressed are: differences in physicochemical composition between mainstream smoke, sidestream smoke and ETS: techniques for field measurement of ETS: relationship between indoor air quality with ETS and its distribution view of US EPA and its problems: biological effects and concerning estimation of ETS exposure.

• 한국연초학회지
• /
• 제4권2호
• /
• pp.67-73
• /
• 1982

Essential oil components from tobacco powder were investigated as flavouring agent. The essential oil was isolated from tobacco powder by a simple distillation /extraction method The extracted essential oil was fractionated into basic, acidic and neutral groups. And the neutral group of essential oil was separated by column chromatography into 10 fractions. Above groups and fractions were tested for tobacco aroma and smoke aroma. The neutral group except most nonpolar fraction displays good flavouring properties which make them highly suitable for improving the flavour and aroma of tobacco and tobacco smoke. The most nonpolar fraction from neutral group was carefully investigated using preparative column, thin layer and gas chromatography ailed by GC/MS coupling. The major subfraction was identified as hydrocarbons on the basis of the IR spectrum. The 58 hydrocarbon components were identified by their mass spectra and was chromatographic retention times.

• 한국연초학회지
• /
• 제18권1호
• /
• pp.76-84
• /
• 1996

Flue-cured tobaccos(cv. NC82, grade AB3O-1) expanded by super steam, volatile organic solvents(tricuorofluoromethane, propane), carbon dioxide, nitrogen, and by freeze-drying were examined for their physico-chemical properties. The expanding rate by using the organic solvents or carbon dioxide at the impregnated pressure was 70% or more, whereas the rate by super steam and freeze-drying was 49.4 and 42.9%, respectively. The contents of total sugar, alkaloid and ether extracts of the expanded tobacco by organic solvents or carbondioxide were decreased to 27.6, 20% or more, and 13%, respectively, when compared to non-expanded one. The contents of citric acid of the tobacco expanded by using trichlorofluoromethane and carbon dioxide were increased to 19.1, 14.0%, respectively. Linoleic acid in the tobacco expanded by super steam or trichlorofluoromethane was also increased to 17.3%, but malonic acid in the tobacco expanded by super steam was 18.5% less than that in the non-expanded one. Nineteen essential oil among 22 assayed in this experiment were decreased in the expanded tobaccos. Key words : expanding method, tobacco, physico-chemical properties.

• 한국식물병리학회지
• /
• 제11권4호
• /
• pp.374-379
• /
• 1995

The cDNA of tobacco mosaic virus-pepper strain (TMV-P) coat protein (CP) genes were introduced into tobacco plants (Nicotiana tabacum cv. Samsun nn) using a binary Ti plasmid vector of Agrobacterium tumefaciens. these cDNAs introduced into tobacco plants were detected by polymerase chain reaction. Symptom development was distinctly suppressed in the transgenic plant introduced buy sense CP cDNA when the plant was inoculated with TMV-P, while in transgenic tobacco plants of antisense CP gene, symptom development was not suppressed as in non-transgenic plants. TMV-P concentration in the sense CP transgenic tobacco plant was decreased to 1/14 of the concentration in non-transgenic plants. Expression of the kanamycin resistance gene of these transgenic plants could be detected in the progeny.

• 한국한의학연구원논문집
• /
• 제13권1호통권19호
• /
• pp.53-61
• /
• 2007

When Ojuyeonmunjangjunsan'go, which we may call the ocean of data in Chosun Dynasty, referes to the matter related tobacco, it often quotes from Enroku. Enroku is a technical book of tobacco in Japan. It not only often quotes from Jibongyuseol, an encyclopedia published in Chosun Dynasty, but also embodies literary works related tobacco written by Koreans. We are so interested, because even the first literary work related tobacco written by a Korean is included in it. But on the ground of foreign book, Koreans have not made a deep study on it. So, In this treatise We will picture a broad outline of Enroku, and introduce literary works related tobacco written by Koreans in it. It would be little aid for the deep study on tobacco itself and on literary works related tobacco in Korea.

• 한국연초학회지
• /
• 제16권2호
• /
• pp.113-121
• /
• 1994

The green peach aphid was relatively the majority(36.9-72.3% ) among all alate aphids collected from tobacco plants. Density of glatae was relatively lower on varieties Tl 1112 and Br 21 than on Va 115 or Y S A among seven varieties tested. However, no considerable difference was observed in general biology of nymphs produced by alatae on the tobacco varieties. About 55% of alate green peach aphid collected from tobacco fields produced progency, and over 80% of the nymphs became adults on cut tobacco leaves in petri dish. Adult longevity of both alate and apteral green peach aphid was shorter, and numbers of nymphs produced by both types were lower in summer than in autumn. Apterae produced more nymphs than alatae, but the longevity of apterae was shorter.

• BMB Reports
• /
• 제32권1호
• /
• pp.1-5
• /
• 1999

In order to understand the biological role of calmodulin in plants, transgenic tobacco plants expressing a calmodulin mutant (VU-4 calmodulin, lys to ile-115) gene have been analyzed. SDS-PAGE and Western-blot analyses showed that the foreign calmodulin mutant is stably and highly expressed in the transgenic tobacco plants. The levels of $H_2O_2$were elevated approximately 2-fold in the transgenic plants. Furthermore, the transgenic tobacco plants have more than 6-fold higher levels of NADPH compared to control tobacco plants. The present findings, combined with previous data showing differences in the susceptibility of the transgenic tobacco seeds and normal tobacco seeds to fungal contamination (Oh and Yang, 1996), suggest that the expression of the calmodulin derivative gene in tobacco plants could increase resistance to infection by fungal pathogens.

• 한국연초학회지
• /
• 제14권1호
• /
• pp.57-65
• /
• 1992

The quality of cut tobacco will be deteriorated due to weather conditions such as temperature and relative humidity, when shipping and long term storaging. The change of equilibrium moisture contents in cut tobacco were under influence of relative humidity and temperature but not humectants. Therefore internal packing materials are necessary for cut tobacco to maintain initial quality, including taste, flavour and musty smell during shipping or storaging. When packing cut tobacco for shipping or long term storaging, desiralbe packing density was 200/$m^2$, For the long term storage under R.H 90% and 3$0^{\circ}C$, polyethylene film thickness suitable for packing material are above 100$\mu$, which resulted in 42days of shelf life.

• 한국연초학회지
• /
• 제2권2호
• /
• pp.51-57
• /
• 1980

담배 모자이크 바이러스(TMV)抵抗性을 가진 T.L448A외 30品種을 供試하여 germplasm의 抵抗性源에 따른 特性을 調査한 結果, 무병징 Group은 국부병반Group에 比하여 長幹, 多收, 晩生種이었으며 品質과 內容成分은 낮았다. 두 group의 諸形質間 相關關係는 開花日數와 草長(r=0.8308), 品質과 還元糖(r=0.9110) 間에 正의 有意相關關係를 보였다. 국부병반 group중 T.I.1504는 TMV抵抗性品種育成에 利用이 可能할 것으로 思料된다.

• 한국연초학회지
• /
• 제7권2호
• /
• pp.151-167
• /
• 1985

Generally, the same quality tobacco may give similar concentration of each chemical component. This research investigation was studied to obtain the differences in concentrations of volatile oil compounds in physically similar tobacco produced in different environment and managements-in Korea and in the United States. The flue-cured leaf tobacco produced in Korea and America was regraded to B3L and P3L by American grading system and analyzed for volatile oils relating to tobacco flavor and aroma. Sixty compounds of the more than 100 peaks distinguishable on the total neutral volatile oils were identified by G5-MS and quantified. Their concentrations are compared between B3 L and P3L produced in Korea and in the United States. The most volatile oil concentrations of B3 L and P3L grade tobacco arc higher in American than in Korean. Only a few components such as benzaldehyde, pulegonc, 4, 6, 9 - megastigmatriene - 3 - one, and coumaran are less in American.