담배 모자이크 바이러스 고추계통(TMV-P)의 외피단백질 유전자를 도입한 형질전환 담배의 TMV-P에 대한 반응

Responses to Infection of Tobacco Mosaic Virus Pepper Strain (TMV-P) in Transgenic Tobacco Plants Expressing the TMV-P Coat Protein or Its Antisense RNA

The cDNA of tobacco mosaic virus-pepper strain (TMV-P) coat protein (CP) genes were introduced into tobacco plants (Nicotiana tabacum cv. Samsun nn) using a binary Ti plasmid vector of Agrobacterium tumefaciens. these cDNAs introduced into tobacco plants were detected by polymerase chain reaction. Symptom development was distinctly suppressed in the transgenic plant introduced buy sense CP cDNA when the plant was inoculated with TMV-P, while in transgenic tobacco plants of antisense CP gene, symptom development was not suppressed as in non-transgenic plants. TMV-P concentration in the sense CP transgenic tobacco plant was decreased to 1/14 of the concentration in non-transgenic plants. Expression of the kanamycin resistance gene of these transgenic plants could be detected in the progeny.