• Biomolecules & Therapeutics
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• 제17권4호
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• pp.422-429
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• 2009

Invasion and metastasis is the main cause of cancer mortality. Angiogenesis is a prerequisite for the tumor growth and metastasis. Matrix metalloproteinases (MMPs) are the key enzymes playing in the invasive growth and metastasis of cancer as well as angiogenesis. Xanthohumol, a prenylated chalcone of the Hop plant (Humulus lupulus L), has been reported to suppress cancer invasion and angiogenesis. In the present study, we investigated the antiinvasive effects of xanthohumol (1) and its synthetic derivatives, 4'-O-methylxanthohumol SEM ether (2), xanthohumol C (3), and xanthohumol C MOM ether (4) in relation to MMP expression in HT-1080 human fibrosarcoma cells. The compound 1 and its derivative, 3 and 4, significantly inhibited serum-induced HT-1080 cell invasion, and 12-O-tetradecanoylphorbol-13-acetate (TPA)-enhanced activity and expression level of MMP-2 and MMP-9 in a concentration-dependant manner. In addition, they inhibited TPA-enhanced expression of MT1-MMP with relatively weak inhibition in tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 level. The compound 1 significantly decreased the cell viability, whereas the derivatives, 2 and 3 showed no cytotoxicity, and compound 4 showed slight cytotoxicity in the cells. Furthermore, in a chick chorioallantoic membrane (CAM) assay, the derivatives 3 and 4 dose-dependently suppressed vascular endothelial growth factor (VEGF)-induced angiogenesis, which is similar to that of compound 1. Taken together, the results indicate that compounds 3 and 4 may be valuable anti-angiogenic agents in the treatment of chronic diseases such as cancer and inflammation working through suppression of MMP-2 and MMP-9.

• Reproductive and Developmental Biology
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• 제31권2호
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• pp.97-102
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• 2007

The objective of this study was to analyzed pattern of proteins expression abnormally in cloned bovine placenta. TIMP-2 protein whose function is related to extracellular matrix degradation and tissue remodeling processes was one of differentially up-regulated proteins in SCNT placenta. And one of down-regulated protein in SCNT placenta was identified as vimentin protein that is presumed to stabilize the architecture of the cytoplasm. The expression patterns of these proteins were validated by Western blotting. To evaluate how regulatory loci. of TIMP-2 and vimentin genes was programmed reprogramming in cloned placenta. the status of DNA methylation in the promoter region of TIMP-2 and vimentin genes was analyzed by sodium Bisulfite mapping. The DNA methylation results showed that there was not difference in methylation pattern of TIMP-2 and vimentin loci between cloned and normal placenta. Histone H3 acetylation state of the nucleosome was analyzed in the cloned placental and normal placenta by Western blotting. A small portion of the protein lysates were subjected to Western blotting with the antibodies against anti acetyl-Histone H3. Overall histone H3 acetylation state of SCNT placenta was significantly higher than those of normal placenta cells. It is postulated that cloned placenta at the end of gestation seems to be unusual in function and morphology of placenta via improper expression of TIMP-2 and vimentin by abnormal acetylation states of cloned genome.

• 대한치과교정학회지
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• 제31권5호
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• pp.499-504
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• 2001

하악골 횡적 성장결핍에 의한 하악 전치부의 총생을 치료하는데 성인의 경우 발치가 종종 요구된다. 그러나 발치에 의한 안모 변화가 바람직하지 않을 때 견인골 신장술을 시행하여 하악 정중부의 확장은 횡적 공간을 확보하여 빠른 교정치료 및 안정성을 확보할 수 있는 방법으로 고려된다. 하악 정중부 견인골 신장술을 시행한 후 tooth-borne type 과 tooth & bone-borne type distractor를 사용하여 latency, distraction, consolidation period를 거쳐 하악 정중부 횡적 확장을 시행하여 전치부 총생을 빠르게 치료할 수 있었으며, 두가지 방법에 의한 치유과정을 비교한 결과 치주적인 요소와 골 형성과정은 유사하였으며, 악관절 부위의 퇴행적 증상변화는 관찰되지 않았다.

• 한국식품영양과학회지
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• 제33권9호
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• pp.1573-1579
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• 2004

단호박을 이용하여 새로운 가공제품을 개발하고자 여러가지 잇점을 줄 수 있는 삼투건조를 최적조건에서 처리하고 산업적으로 많이 사용되고 있는 열풍, 진공건조와 새로운 건조방법의 적용을 위해 선정한 냉풍건조를 행한 후 품질특성을 비교 평가하였다. 삼투처리 후 열풍, 진공, 냉풍건조된 단호박의 수분함량은 sucrose로 삼투처리한 경우가 모든 건조 방법에서 glucose 처리구보다 낮았으며, 건조방법에 따른 비타민 C의 함량은 glucose로 삼투처리 후 냉풍건조한 경우가 35.69 ㎎/100 g으로 가장 높게 측정되었다. 전반적으로 침지 용액에 따른 L, a, b값은 유사한 경향이었으며, 냉풍건조가 색도변화가 가장 작게 일어나 생단호박의 색상과 유사하였다. 건조 단호박의 표면구조는 삼투용액과 건조방법에 따라 상이하였는데, 삼투처리 후 냉풍건조된 단호박을 제외하고 열풍 및 진공건조된 단호박의 조직 손상이 크게 나타났다. 건조방법 및 건조시간에 따른 수분함량과 비타민 C함량을 살펴보면 수분함량의 경우 건조효율성을 고려할 때 sucrose로 삼투처리 후 진공건조한 단호박이 가장 우수하였으나, 비타민 C함량에서는 glucose로 삼투처리 후 냉풍건조한 단호박이 가장 양호하였다. 삼투용액에 따른 건조방법별 건조속도는 sucrose로 삼투처리한 단호박의 건조속도가 glucose 처리구보다 빠름을 알 수 있었다.

• 식물조직배양학회지
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• 제25권1호
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• pp.21-25
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• 1998

미치광이풀(Socopolia parviflora Nakai) 모상근 배양에서 모상근 생장과 tropane alkaloid 생산에 대한 sucrose농도와 일부 흡착제의 효과를 조사하였다. 모상근 클론 SP 11은 5% sucrose 농도를 함유한 1/2 B5 배지에서 최적인 생장과 tropane alkaloid 생산을 보였다. 모상근에서 tropane alkaloids 생성양상은 모식물에서와 약간의 차이를 보였고 그 중 식물체에서 검출되지 않는 littorine의 생성이 모상근에서 두드러졌다. 조사된 흡착제(0.01% 1%) 중에서, charcoal 0.01%와 XAD-II l%에서 약간의 생장촉진효과를 보인 것을 제외하고는 그 외 농도나 흡착제(amberlita chitosan)에서는 억제효과 또는 유의적인 변화를 보이지 않았다. 반면에 hydroxyapatite는 미치광이풀 모상근의 생장과 tropane alkaloid 생산에 있어 촉진효과가 있음이 확인되었다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제19권5호
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• pp.35-44
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• 2014

In this study, the bio-phytoremediation and phytoremediation technologies were applied to the soils contaminated with total petroleum hydrocarbons (TPH) and heavy metals to evaluate the remediation efficacy from May 2012 to December 2013. Poplar (Populus bonatii Levl.) and Sun Hemp (Crotalaria juncea L.) were selected and planted in phytoremediation practice. These plants were also utilized in the bio-phytoremediation practice, with the addition of earthworm (Eisenia fetida) and petroleum-degrading bacteria (Pseudomonos sp. NKNU01). Furthermore, physiological characteristics, such as photosynthesis rate and maximal photochemical yield, of all testing plants were also measured in order to assess their health conditions and tolerance levels in adverse environment. After 20 months of remedial practice, the results showed that bio-phytoremediation practice had a higher rate of TPH removal efficacy at 30-60 cm depth soil than that of phytoremediation. However, inconsistent results were discovered while analyzing the soil at 100 cm depth. The study also showed that the removal efficiency of heavy metals was lower than that of TPH after remediation treatment. The results from test field tissue sample analysis revealed that more Zinc than Chromium was absorbed and accumulated by the tested plants. Plant height measurements of Poplar and Sun Hemp showed that there were insignificant differences of growth between the plants in remediation plots and those in the control plot. Physiological data of Poplar also suggested it has higher tolerance level toward the contaminated soils. These results indicated that the two testing plants were healthy and suitable for this remediation study.

• Journal of Chest Surgery
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• 제36권10호
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• pp.794-797
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• 2003

광역동치료는 흉부외과 영역에서 조기 식도암, 기관 및 기관지 종양, 위장관 점막 내 종양 및 바레트식도 등에서 광감작물질의 광파장 및 광선의 암세포에 대한 선택적이고 직접적인 독성 효과를 이용한 비수술적 치료로 안전하고 효과가 있으며, 기타 진행된 폐암이나 식도암에서 보존적 요법으로도 이용될 수 있다고 여겨지고 있다. 그러나 광역동치료 후 광독성에 의한 피부 독성이 흔하여, 직사 광선의 차단 및 약물 요법이 병행되어야 한다. 저자들은 식도암 수술 후 흘곽 내에 거치된 위장에 발생한 원발성 위암에 대한 광역동치료(Photodynamic tharapy)로 좋은 결과를 얻었기에 문헌 고찰과 함께보고하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권7호
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• pp.855-862
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• 2010

Among the known interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful pathways. The Mx protein has direct antiviral activity and inhibits a wide range of viruses by blocking an early stage of the viral replication cycle. Cloning, characterization, and expression of Mx in vivo and in vitro have been conducted. The chicken Mx gene spans 21 kb and is made up of 14 exons and 13 introns, of which the promoter region was analyzed. The real-time PCR results showed that Mx expression was increased in chicken embryo fibroblasts (CEF) after 12- and 24-h induction with polyI: C. Induction of Mx expression by poly I: C in vivo revealed tissue-specific patterns among the chicken tissues tested. A trace expression of Mx was detected in healthy chicken liver tissues from adult chickens without inducement; the expression levels in the liver, heart, and gizzard were higher than in the muscle and kidney. This is the first report to demonstrate the expression of a glutathione-S-transferase-tagged-Mx fusion protein of 75 KDa, as well as the biological activity tested by SDS-PAGE and western blotting.

• Nutrition Research and Practice
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• 제10권2호
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• pp.148-153
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• 2016

BACKGROUND/OBJECTIVES: Bone formation and bone resorption continuously occur in bone tissue to prevent the accumulation of old bone, this being called bone remodeling. Osteoblasts especially play a crucial role in bone formation through the differentiation and proliferation. Therefore, in this study, we investigated the effects of Scytosiphon lomentaria extract (SLE) on osteoblastic proliferation and differentiation in MC3T3-E1 cells. MATERIALS/METHODS: A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining and protein expression analysis of osteoblastic genes were carried out to assess the osteoblastic proliferation and differentiation. RESULTS: The results indicated that treatment of SLE promoted the proliferation of MC3T3-E1 cells and improved ALP activity. And, SLE treatment significantly promoted mineralized nodule formation compared with control. In addition, cells treated with SLE significantly upregulated protein expression of ALP, type 1 collagen, bone morphogenetic protein 2, runt-related transcription factor 2, osterix, and osteoprotegerin. CONCLUSIONS: The results demonstrate that SLE promote differentiation inducement and proliferation of osteoblasts and, therefore may help to elucidate the transcriptional mechanism of bone formation and possibly lead to the development of bone-forming drugs.

• Current Research on Agriculture and Life Sciences
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• 제10권
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• pp.137-145
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• 1992

본 연구에 사용한 시료는 형성층 유래의 캘러스를 0.01mg/${\ell}$ 2, 4-D, 0.5mg/${\ell}$ BAP, 3% sucrose, 0.75% 한천을 첨가한 MS배지에서 재분화 시킨 개체를 이용하였다. 시료의 대량증식은 1.0mg/${\ell}$ BAP를 첨가한 MS 배지에서 실시하였으며 엽전개는 식물생장조절제가 첨가되지 않은 MS배지로 옮겨서 실행하였다. Agrobacteria를 이용한 형질 전환은 엽절편, 절간조직등을 박테리아를 묻힌 침으로 자극하여 식물체 분화를 유도하였다. 그 결과 엽절편 조직에서는 분화된 식물체를 얻지 못했으나, 절간조직의 측아에서는 49%에 달하는 24개체가 분화되었다. 이들 분화된 줄기는 kanamycin이 100mg/${\ell}$이 함유된 선발 배지에서 일차적인 선발을 하여 최종적으로 GUS 유전자 검정을 한 결과 처음에 접종한 50개체중 형질전환 된 것으로 추정되는 5개체를 얻어서 형질 전환 추정 비율은 10%에 달한 것으로 나타났다.