• Journal of Biomedical Engineering Research
• /
• v.23 no.1
• /
• pp.61-72
• /
• 2002

In this parer, a new harmonic imaging technique is Proposed and evaluated experimentally. In the Proposed method. a weighted chirp signal with a hanning window is transmitted. The RF samples obtained on each array element are individually compressed by correlating with the reference signal defined as the 2nd harmonic component($2f_0$) of a transmitted chirp signal generated in a square-law system. The correlator output will then consist of the compressed version of the $2f_0$ component generated in tissue and the crosscorrelation sequence of the fundamental($f_0$) and 2f$_{0}$components. The Proposed method uses the compressed $2f_0$ component to form an image. for which the crosscorrelation term should be suppressed below at least -50dB. The Proposed method has two process, 2f$_{0}$-correlation and $2f_0$-correlation(PI) . $2f_0$-correlation can successfully eliminate the $f_0$ component with a single transmit-receive events and therefore is more efficient than the conventional Pulse inversion method in terms of the frame rate. 2i)-correlation(Pl) Performs pulse compression after applying pulse inversion method for the 2nd harmonic image with high resolution and SNR. Another advantage of the proposed method is that the SNR of 2nd harmonic imaging can be improved without limitation by increasing the duration of the chirp signal. The proposed method was verified through both the computer simulations and actual experiments .ts .

• Journal of Life Science
• /
• v.26 no.9
• /
• pp.1097-1104
• /
• 2016

With the development of next generation sequencing (NGS), large numbers of transcriptional molecules have been discovered. Most transcripts are non -coding RNAs (ncRNAs). Among them, long non-coding RNAs (lncRNAs) with more than 200 nucleotides represent functional RNA molecule that will not be translated into protein. In plants, lncRNAs are transcribed by RNA polymerase II (Pol II) or Pol III, Pol VI and Pol V. After transcription of these lncRNAs, more RNA processing mechanisms such as splicing and polyadenylation occurs. The expression of plant lncRNAs is very low and is tissue specific. However, these lncRNAs are strongly induced by specific external stimuli. Because different external stimuli including environmental stresses induce a large number of plant lncRNAs, these lncRNAs have been gradually considered as new regulatory factors of various biological and development processes such as epigenetic repression, chromatin modification, target mimicry, photomorphogenesis, protein relocalization, environmental stress response, pathogen infection in plants. Moreover, some lncRNAs act as precursor of short RNAs. Although a large number of lncRNAs have been predicted and identified in plants, our current understanding of the biological function of these lncRNAs is still limited and their detailed regulatory mechanisms should be elucidated continuously. Here, we reviewed the biogenesis and regulation mechanisms of lncRNAs and summarized the molecular functions unraveled in plants.

• Korean Journal of Food Science and Technology
• /
• v.29 no.4
• /
• pp.758-763
• /
• 1997

Fresh jujubes (Zizyphus jujuba Miller) of whitish green and red ripe maturities were stored at 5 different temperatures, and quality changes through the storage were measured to find an optimal storage condition. Respiration rates and their temperature dependences for both maturities were not different from each other, which suggested non-climacteric pattern of postharvest respiration. Red ripe fruits showed heavier weight and higher content in soluble solid and ascrobic acid compared with whitish green mature fruits. Through the storage of jujubes in perforated packages tissue softening and decay were main visual quality deteriorations with the former preceding the latter. The whitish green mature jujubes showed slower rate of quality changes in softening and decay than red ripe ones, and are thus more suitable for long term storage. In the storage, the whitish green fruits changed into red color, and showed increase in soluble solid and decrease in acidity and ascorbic acid content. Storing the jujubes at $-2^{\circ}C$ resulted in symptoms of chilling injury, and storing at higher temperatures above $0^{\circ}C$ accelerated the decay and softening. $0^{\circ}C$ was found to be optimal temperature for long term storage, where jujube had the lowest rate of quality changes without chilling injury. Even at optimal temperature of $0^{\circ}C$, however, storage life retaining freshness was only around 40 days which is not enough.

• Journal of Biomedical Engineering Research
• /
• v.18 no.4
• /
• pp.429-438
• /
• 1997

Mechanical valve is one of the most widely used implantable artificial organs of which the reliability is so important that its failure means the death of patient. Therefore early noninvasive detection is essentially required, though mechanical valve failure with thrombosis is the most common. The objective of this paper is to detect the thrombosis formation by spectral analysis and neural network. Using microphone and amplifier, we measured the sound from the mechanical valve which is attached to the pneumatic ventricular assist device. The sound was sampled by A/D converter(DaqBook 100) and the periodogram is the main algorithm for obtaining spectrum. We made the thrombosis models using pellethane and silicon and they are thrombosis model on the valvular disk, around the sewing ring and fibrous tissue growth across the orifice of valve. The performance of the measurment system was tested firstly using 1 KHz sinusoidal wave. The measurement system detected well 1KHz spectrum as expected. The spectrum of normal and 5 kinds of thrombotic valve were obtained and primary and secondary peak appeared in each spectrum waveform. We find that the secondary peak changes according to the thrombosis model. So to distinguish the secondary peak of normal and thrombotic valve quantatively, 3 layer back propagation neural network, which contains 7, 000 input node, 20 hidden layer and 1 output was employed The trained neural network can distinguish normal and valve with more than 90% probability. As a conclusion, the noninvasive monitoring of implanted mechanical valve is possible by analysing the acoustical spectrum using neural network algorithm and this method will be applied to the performance evaluation of other implantable artificial organs.

• Journal of Periodontal and Implant Science
• /
• v.31 no.3
• /
• pp.543-554
• /
• 2001

치주조직 재생을 도모하기 위한 전통적인 시술방법으로 여러가지 골이식재를 이용한 골이식술이 이용되고 있다. 이번 실험의 목적은 골형성을 위한 재료로 Calcium Polyphosphate(CPP)를 사용하여 비글견에서의 조직 반응과 골유도성을 보는 것이고 또한 다른 이식재들간에 신생골의 형성에 어느정도 영향을 주는 가를 알아보는 것이다. 이번 실험에 사용된 CPP는 무수 $Ca(H_2PO_4)$를 condensation하여 무결정의 $Ca(PO_3)_2$를 얻고 이를 용율하고 냉각시킨 후 분쇄하여 얻은 것으로 3세된 비글견에 이식하여 관찰하였다. 조직학적으로 CPP granule의 경우는 키토산이나 $Na_2O$를 넣은 경우 모두 bone 의 ingrowth가 관찰되었고 다른 섬유조직의 개재를 볼수 없었다 동결탈회건조골을 넣은 경우에는 주위로 골형성 보이지 않았고 단지 섬유성 조직이 관찰 되었다. 아무것도 넣지 않은 경우에 비해서 동결탈회건조골이나 키토산, $Na_2O$를 넣은 CPP granules 경우에 더 많은 비율로 신생 골의 양이 나타나는 것을 볼수 있었다. 아무것도 넣지 않은 대조군과 이식재를 넣은 군간에는 유의성이 있는 것으로 나타났고(p<0.05). 또한 CPP granules with chitosan과 CPP granules with $Na_2O$ 사이에는 신생골의 형성에 유의성이 없었다. 이것으로 보아 CPP granules with chitosan과 CPP granules with $Na_2O$는 모두 골유도성이 있고 신생골의 형성을 촉진하므로 치주질환으로 인한 골결손 부위에 사용할 수 있는 재료로 우수한 특성을 지닌다고 사료된다.

• Journal of Navigation and Port Research
• /
• v.40 no.1
• /
• pp.7-14
• /
• 2016

Although the life jacket can provide the buoyance with the drowner, heat loss can make the drowned individual be subject to the hypothermia. In this study, The thermal insulation of two types life jacket including inflatable and foam type were evaluate by both experiments and numerical analysis. To estimate the thermal resistance of the jackets, experiments on the heat flux were conducted by the thermal manikin exposed to cold water. Heat flux loss on the surface of thermal manikin were measured for both foam and inflatable type life jacket. Also, finite element method is applied to a body section in order to understand the level of hypothermia of each life jacket. The segmental of human thigh is represented by a multi-layered section which considers the heat conduction within tissue, bone and fat. As a result, the thermal resistance and hypothermia time of each jackets have been compared based on the finite element analysis. It was found that the insulation ability of suggested life jackets is better than that of conventional type.

• Journal of the Optical Society of Korea
• /
• v.20 no.5
• /
• pp.607-613
• /
• 2016

The pleura is known as an end target organ of exposure to toxic environmental materials such as fine particulate matter and asbestos. Moreover, long-term exposure to hazardous materials can eventually lead to fatal lung disease such as diffuse pleural fibrosis or mesothelioma. Chest computed tomography (CT) and ultrasound are gold standard imaging modalities for detection of advanced pleural disease. However, a diagnostic tool for early detection of pleural reaction has not been developed yet due to difficulties in imaging ultra-fine structure of the pleura. Optical coherence tomography (OCT), which provides cross-sectional images of micro tissue structures at a resolution of 2-10 μm, can image the mesothelium with a thickness of ~100 μm and therefore enables investigation of the early pleural reaction. In this study, we induced the early pleural reaction according to a time sequence after pleurodesis using talc, which has been widely used in the clinical field. The pleural reaction in talc grouped according to the time sequence (1st, 2nd, 4th weeks) showed a significant thickening (average thickness: 45 ± 7.5 μm, 80 ± 10.7 μm, 90 ± 12.5 μm), while the pleural reaction in sham and normal groups showed pleural change from normal to minimal thickening (average thickness: 16 ± 5.5 μm, 17 ± 4.5 μm, 15 ± 6.5 μm, and 12 ± 7.5 μm, 13 ± 2.5 μm, 12 ± 3.5 μm). The measurement of pleural reaction by pathologic examinations was well-matched with the measurement by OCT images. This is the first study for measuring the thickness of pleural reactions using a biophotonic modality such as OCT. Our results showed that OCT can be useful for evaluating the early pleural reaction.

• Journal of Life Science
• /
• v.17 no.6 s.86
• /
• pp.822-824
• /
• 2007

The efficient system for plant regeneration from cotyledon and primary feat explants of lettuce was established. Plant regeneration efficiency was shown 91.3% from cotyledon and 85.9% from primary leaf explants of variety 'Jungtongpogi' in KN medium. Plant regeneration efficiency was also estimated with various plant regeneration media in variety' Chungchima', which was lowest plant regeneration efficient showing 35.4% from cotyledon and 30.3% from prima leaf explants in KN medium. Kl medium increased 77.9% and 80.7% of plant regeneration efficiencies from cotyledon and primary leaf explants of variety 'Jungtongpogi' were cultured on KN medium. In case of varie쇼 ‘Chungchima', efficient plant regeneration was shown when primary leaf explants were cultured on SH and KI media.

• Journal of Biomedical Engineering Research
• /
• v.25 no.6
• /
• pp.431-438
• /
• 2004

Medical ultrasonic imaging is a useful imaging facility known to be most safe and easy. It enables physicians to observe the inside structures of the bodies, blood flow, and motions of internal organs. Some physical properties of biologic tissues can also be estimated from backscattered sounds. However, the ultrasonic pulses interrogating the living organisms leave their footprints in the returning signals during imaging. Some significant details are buried in the footprints and their overlaps from adjacent particles. These distortions also decrease the quality of the images. Many research efforts have been made to enhance the image quality and to recover the acoustic information in various ways. In this study, a new interrogation method based on the wavelet and subband filter bank is proposed. It adopts the subband wavelet filters satisfying the perfect-reconstruction (PR) conditions as the interrogating pulses to restore the details useful in tissue characterization and to enhance the image quality. The proposed method was applied to two types of simulations of ultrasonic imaging. The results showed its ability to restore the detailsin the simulated interrogation of biologic tissues, and verified the improved image quality in the simulated imaging of general ultrasonic phantom compared with the conventional method.

• Journal of Navigation and Port Research
• /
• v.39 no.4
• /
• pp.285-291
• /
• 2015

Exposure to cold sea water can be life-threatening to the drowned individual. Although appropriate life jacket can be usually be provided for the buoyance at the drowning accident, heat loss can make the drowned individual experience the hypothermia. Inflatable life jackets filled with inflatable air pocket can increase the thermal protection as well as the buoyancy force. Because it is important to know how the human body behaves unde the different life jacket, present study compares the thermal insulation capacity of solid type life jacket with that of inflatable life jacket. In order to represent the insulation capacity of life jacket, thermal resistance is estimated based on the assumption of steady-state. Also, a transient three-dimensional thermal distribution of the thigh is analyzed by using finite element method implementing the Pennes bioheat equation. The finite element model is a segmental, multi-layered representation of the body section which considers the heat conduction within tissue, bone, fat and local blood flow rate.