Two kinds of anticaking agents (dextrin, polydextrose) were combined with kiwifruit paste at 5% w/w ratio and freeze-dried to prepare a powdered material. The physiochemical characteristics of kiwifruit powders with anticaking agents were compared with those without anticaking agents as the control. The yield was higher in the powders with anticaking agents than the control. Moisture content, acidity, and total phenolics were lower in the powders with anticaking agents than the control. The contents of vitamin C was higher in the powders with anticaking agents than the control, but was no significant difference with different anticaking agent types. There were no significant differences in free sugar content (fructose, glucose, total sugar) and organic acid content (oxalic acid, lactic acid, total) depending on the anticaking agent types. Hunter's L-value was significantly high in the order of the samples with dextrin, the control, and polydextrose, while a-value showed an opposite tendency. Browning index, water solubility, and swelling power didn't show any significant difference. However, the hygroscopicities with elapsed time were lower in the powders with anticaking agents than the control. Therefore, the kiwifruit powder combined with dextrin or polydextrose as an anticaking agent at 5% w/w ratio could be used as a food biomaterial with a good quality in moisture, vitamim C, color value, browning index, water solubility, and hygroscopicity.
The purpose of this study was to investigate the physiological response to salt treatments in the leaves of several tree species. The results obtained were as follows : 1. The water potential of tree leaves damaged with various salt concentrations did not change nearly for 10 hours after treatment. As time elapsed after treatment, however, the higher salt concentration in soils, the higher leaf water potential was observed. 2. Leaf water potential of species intolerant to salt was higher than tolerant species due to the severe dehydration from cells. 3. According to the water relation parameters obtained from P-V curves, the values of ${\pi}_{\sigma}$ and ${\pi}_{\rho}$ in the damaged leaves were higher, but those of $V_{\rho}/V_{\sigma}$ and Emax were lower than those of the intact leaves. 4. The photosynthesis rate of tree leaves decreased remarkably with increasing the salt concentrations in soils, and it decreased faster for species intolerant cintolerant to salt.
Several experiments including persistence, distribution, leaching, and terminal residue trials were carried out to investigate the behavior of cyclosulfamuron in rice paddies. Cyclosulfamuron was gradually dissipated in two different soils showing the first-order kinetics. Half-lives of the herbicide were calculated to be $17{\sim}30$ and $14{\sim}16$ days under field and laboratory conditions, respectively. In the paddy soil/water system, the residue tended to reside more in the soil phase as time elapsed. Cyclosulfamuron was less persistent in paddy water than in soil with half-lives of 10 and 19 days, respectively. No cyclosulfamuron was leached below 20 cm-deep soil during water percolation with 50 cm hydraulic head, while some downward mobility was observed within the soil column. When EC and SC formulations of cyclosulfamuron were applied to the paddy field at 120 or 150-day pre-harvest intervals, its terminal residues in hulled rice were all less than 0.01 mg/kg, irrespective of formulation type and application timing. In rice straw, however, some residues were found at $<0.02{\sim}0.05$ mg/kg as SC formulation was applied. Rapid dissipation, restricted mobility, and low terminal residues of cyclosulfamuron in rice paddies suggest that no significant residues would be transported or carried over to the non-target environment.
This study was made to optimize the conditions needed to produce two types of gynogenetic diploids in the sweet fish, Plecoglossus altivelis. Firstly, ultraviolet (UV) ray doses between 3,000 erg to 14,000 erg/$\textrm{mm}^2$ were tested to inactivate sperm genetically. Based on the appearance of the haploid syndromes in the embryo, a dose of UV ray 6000~7000 erg was required to inactivate sperm genetically. Then, cold shock treatment at 1~2$^{\circ}C$ for 15~30 min were conducted to retain the 2nd polar body in inseminated egg. The best elapsed time before the start of the cold shock was examined between 5~8 min. The experiments in which began 5 min after insemination at 1~2$^{\circ}C$ during 17.5 min gave 21.2% survival rate and 89.7% normal eyed embryo rate. The gynogenetic diploid produced by suppression of the first cleavage, a considerably high number of heteroploids appeared and high mortality was observed at the metamorphosis stage, so further investigation is needed. The production of gynogenetic diploids were confirmed by GPI isozyme marker. The heterozygous type in Gpi-1 locus was observed in the meiotic-G2N as a result of gene-centromere recombination during meiosis. The heterozygous type was never observed in mitotic-G2N and showed segregation into two homozygous types at Gpi-1 locus.
This study was conducted to investigate the change of antioxidant enzyme activity (catalase and superoxide dismutase) and variation of blood physiology in olive flounder (Paralyticus olivaceus) by hydrogen peroxide (H2O2) treatment. Blood parameters were measured 1, 3 and 5 hours after H2O2 treatment with 0 (control), 100, 300 and 500 ppm for 1 hr. The value of hematocrit was decreased significantly dependently on treatment concentrate and elapsed time in the treatment of H2O2. Hemoglobin concentration in the test groups were lower than that of the control group. Red blood cell value in the test groups were significantly lower compared to that of the control group, but recovered to the level of the control group after 5 hr. Protein concentration was significantly lower compared to that of the control group at 0 and 1 hr, but recovered after 3 hr in 500 ppm treatment group. The superoxide dismutase (SOD) and catalase (CAT) enzyme activities were observed to be increased. Heat-shock protein 70 (HSP70) was significantly increased compared to that of control group in all of the test groups. HSP70 mRNA groups was highly expressed in 500 ppm treatment.
The average concentration of Pb in 53 samples of canned orange juice currently sold in Korean market was 0.225 mg/kg, and that of Sn, 40.7 mg/kg. There was no appreciable difference in Pb concentration according to elapsed time after manufacturing, whereas Sn concentration increased 0.66 mg/kg per month. During the storage at room temperature or in refrigerator after opening, the Pb concentration increased slowly, reaching 1.7 to 1.8 times of original concentration, whereas Sn concentration increased by 20% per day, resulting in 3 times of original concentration after 7 days. There were no serious changes in Pb and Sn concentration in storage at room temperature or refrigerator for 3 days, when juice samples were opened and transferred to glass container. It is needed that detailed inspection by undertaken to monitor the contents of heavy metals in canned orange juice, since 18% of samples within recommended distribution period exceeded the legal standard for Pb, and recommended that more attention be paid in handling canned orange juice after opening, in order to avoid the hazard from heavy metals.
Recently, many efforts have been made to construct the first unlined tunnel, without in-situ concrete lining, in Korea. However, the lack of reliability in the performance of shotcrete as permanent tunnel support prevented from its realization. Shotcrete has been regarded to have significant problems in field application and long term performance because of unsatisfactory strength level and durability compared to those of European countries. In this study, the high strength shotcrete satisfying compressive strength over 40 MPa and flexural strength over 4.5 MPa was developed from optimized mix design. The type of accelerators and the amount of silica fume were selected as the main factors in mixing process and the analyses were carried out up to the elapsed time of 2 years. In order to evaluate the short term durability of shotcrete, an array of laboratory test consisting of freeze-thaw, carbonation chloride penetration and permeability test was performed. For long-term durability tests, specimens have been put in an operated highway tunnel to expose them to the similar environment when they are actually used as an unlined tunnel support. From the strength and durability tests, it was found that only alkali-free based accelerator satisfied the target strength of this study and also, the developed shotcrete showed very high performance in its durability.
Nutrients and water absorption by Citrus unshiu Marc. cv. Miyagawa Wase were examined using spray type of out-door hydroponics system. Three different concentration levels of nutrient solution were treated to citrus trees to examine nutrient uptake. To do so, concentration and the volume change of nutrient solution was measured for each treatment. By weighing the volume of solution and citrus trees, amounts of water uptake and fresh weight increase were observed periodically. Water uptake by trees increased as tree grew and time elapsed, but was lower at high level of nutrient concentration than the rest, due partly to the reduction in hydraulic conductance and to the sustained high salt concentration. Rapid increase in fresh weight and nutrient uptake occurred from May to July and from August to September. The amounts of nutrient uptake were significantly different among nutrient levels: the higher concentration, the greater uptake by citrus tree. The absorbed amounts of $NO_3$, K and Ca were much higher than those of $NH_4$, S, P, Mg, and Fe. Most mineral contents in leaves were proportional to the concentration of supplied nutrient solutions. From the results of nutrient absorption and contents in leaves at different levels of nutrient solutions, the composition of major elements for citrus nutrient solution can be modified as follows: 27.1, 16.5, 66.0, 80.0, and $24.0mg\;L^{-1}$ for N, P, K, Ca, and Mg, respectively.
We used green manure crops such as hairy vetch, crimson clover, rye, sorghum, and sudan grass by mixing with soils to assess the effects of green manure crops on nutrient supply and soil quality improvement. Temporal changes in soil inorganic nitrogen, carbohydrate, microbial biomass, and humus content were determined as soil quality indicators. Inorganic nitrogen content of the control maintained similar level during the whole period, but it had continually increased until 4 weeks after incorporation (WAI) of green manure crops. Especially, inorganic nitrogen content sharply increased in sudan grass. After incorporation of green manure crops, temporal change of soluble sugar in soils was as follows: it had gradually increased in legume green manure crops-incorporated soils until 7 WAI, which was the highest, and then showed the tendency to be reduced. Meanwhile, it in non-legume green manure crops-incorporated soils rapidly increased after the incorporation, and reached the maximum around 4 WAI. Humic acid by the decomposition of crop residues in green manure crops-incorporated soils was greatly enhanced with the elapsed time of 4 WAI, although it was low at the same level as the control until 2 weeks. In addition, there was a difference in fulvic acid by incorporated crops, fulvic acid in hairy vetch, sorghum and sudan grass showed a similar tendency with the change in humic acid. Our results suggest that soluble sugar, microbial activity and humic acid could be available indicators to evaluate the fertility of green manure crops-incorporated soils.
This report compared paramedian and flank approaches for the effective removal of abdominal unilateral cryptorchidism in calves. Two Korean native calves of approximately 300 kg in body weight, which had undergone the removal of a testis from the scrotum, were diagnosed as having abdominal unilateral (left) cryptorchidism by rectal palpation. One calf was administered with xylazine (0.2 mg/kg, IV) and was restrained in the dorsal recumbency position on the ground of barn for the paramedian approach, while the other calf was sedated with xylazine (0.03 mg/kg, IV) and restrained in the standing position for a flank approach. For the two calves, 2% lidocaine was administered by local infiltration, along the proposed incision line. Following incisions of the skin, muscle and peritoneum, the testis, which was located between the left kidney and inguinal canal, was grasped, exteriorized out of the abdominal cavity and detached from the gubernaculum testis. Then spermatic cord was ligated and cut. Finally, the testis was removed. An assistant was required to restraint a calf in dorsal recumbency for paramedian approach, and cryptorchidectomy was carried out with operator in a rather uncomfortable squatting posture, while restraint was rather simple and cryptorchidectomy was proceeded with operator in a standing posture where the flank approach was taken. During surgery, the gubernaculum testis was more strained during pulling the testis in the flank approach than in the paramedian approach, as the distance between incision site and the gubernaculum testis was greater in the flank approach than that in the paramedian approach. On the other hand, intra-abdominal pressure was greater in the paramedian approach than in the flank approach during surgery. The elapsed time between restraint and the end of surgery was 10 min shorter where the flank approach (35 min) was taken, than where the paramedian approach (45 min) was taken. In conclusion, this case study demonstrated that the flank approach might be more convenient than the paramedian approach for abdominal unilateral cryptorchidectomy in calves.
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