• Title/Summary/Keyword: three-dimension culture

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Study on torso body types of adult males over 178cm tall (178cm 이상의 키 큰 성인남성에 대한 체간부 체형 유형화 연구)

  • Kim, Min-jung
    • The Research Journal of the Costume Culture
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    • v.30 no.5
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    • pp.675-688
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    • 2022
  • The purpose of this study is to analyze the body type characteristics of tall adult men aged 20 to 69 years to present base material on body dimensions for men's clothing companies. The research method comprised statistical analysis of 3D measurements after selecting items from the 8th Korean human body size survey related to the torso body types of 495 adult men who were at least 178cm tall. As a result, six factors were extracted, and the total explanatory variable of the entire factor was shown to be 80.499%. As a result of cluster analysis using this as an independent variable, three types were derived: Type 1, "a normal body type with an oval cross-sectional shape" (37.6%), with a larger vertical size and an oval chest cross-sectional shape compared to other types; Type 2: a "cylindrical thick body shape" (31.1%), which is the first stage of BMI obesity, with narrow shoulders and a cylindrical shape with a round cross-section; Type 3, "a body shape with broad shoulders and developed chest parts" (31.3%), with a horizontal size similar to Type 2 above the waist, but similar to Type 1 below. The results of this study are expected to be used as a basis for a clothing dimension system and clothing development for tall men reflecting the characteristics of the torso.

Effects of Extracellular Signaling on the Endogenous Expression of Self-Renewal-Stimulating Factor Genes in Mouse Embryonic Stem Cells

  • Gong, Seung-Pyo;Lee, Seung-Tae
    • Reproductive and Developmental Biology
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    • v.36 no.1
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    • pp.13-19
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    • 2012
  • In order to provide the basis for developing practical mouse embryonic stem cells (mESCs) culture method, how the endogenous level of self-renewal-stimulating factor genes was altered in the mESCs by different extracellular signaling was investigated in this study. For different extracellular signaling, mESCs were cultured in 2 dimension (D), 3D and integrin-stimulating 3D culture system in the presence or absence of leukemia inhibitory factor (LIF) and transcriptional level of $Lif$, $Bmp4$ and $Wnt3a$ was evaluated in the mESCs cultured in each system. The expression of three genes was significantly increased in 3D system relative to 2D system under LIF-containing condition, while only $Wnt3a$ expression was increased by 3D culture under LIF-free condition. Stimulation of integrin signaling in mESCs within 3D system with exogenous LIF significantly up-regulated transcriptional level of $Bmp4$, but did not induce transcriptional regulation of $Lif$ and $Wnt3a$. In the absence of LIF inside 3D system, the expression of $Lif$ and $Bmp4$ was significantly increased by integrin signaling, while it significantly decreased $Wnt3a$ expression. Finally, the signal from exogenous LIF significantly caused increased expression of $Lif$ in 2D system, decreased expression of $Bmp4$ in both 2D and 3D system, and decreased expression of $Wnt3a$ in integrin-stimulating 3D system. From these results, we identified that endogenous expression level of self-renewal-stimulating factor genes in mESCs could be effectively regulated through artificial and proper manipulation of extracellular signaling. Moreover, synthetic 3D niche stimulating endogenous secretion of self-renewal-stimulating factors will be able to help develop growth factor-free maintenance system of mESCs.

Development of a Three-dimensional Hydrogel System for the Maintenance of Porcine Spermatogonial Stem Cell Self-renewal

  • Park, Ji Eun;Park, Min Hee;Kim, Min Seong;Yun, Jung Im;Choi, Jung Hoon;Lee, Eunsong;Lee, Seung Tae
    • Journal of Embryo Transfer
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    • v.32 no.4
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    • pp.343-351
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    • 2017
  • Porcine spermatogonial stem cells (SSCs) prefer three-dimensional (3D) culture systems to 2D ones for the maintenance of self-renewal. Of the many 3D culture systems, agar-based hydrogels are candidates for supporting porcine SSC self-renewal, and there are various types of agar powder that can be used. In this study, we sought to identify an agar-based 3D hydrogel system that exhibited strong efficacy in the maintenance of porcine SSC self-renewal. First, 3D hydrogels with different mechanics were prepared with various concentrations of Bacto agar, lysogeny broth (LB) agar, and agarose powder, and the 3D hydrogel with the strongest alkaline phosphatase (AP) activity and greatest increase in colony size was identified for the different types of agar powder. Second, among the porcine SSCs cultured in the different 3D hydrogels, we analyzed the colony formation, morphology, and size; AP activity; and transcription and translation of porcine SSC-related genes, and these were compared to determine the optimal 3D hydrogel system for the maintenance of porcine SSC self-renewal. We found that 0.6% (w/v) Bacto agar-, 1% (w/v) LB agar-, and 0.2% (w/v) agarose-based 3D hydrogels showed the strongest maintenance of AP activity and the most pronounced increase in colony size in the culture of porcine SSCs. Moreover, among these hydrogels, the strongest transcription and translation of porcine SSC-related genes and largest colony size were detected in porcine SSCs cultured in the 0.2% (w/v) agarose-based 3D hydrogel, whereas there were no significant differences in colony formation and morphology. These results demonstrate that the 0.2% (w/v) agarose-based 3D hydrogel can be effectively used for the maintenance of porcine SSC self-renewal.

Factors Affecting Overall Satisfaction on Car Sharing Service: Satisfaction on Service Quality, Satisfaction on Service Product, Satisfaction on Service Experience (카쉐어링 서비스 종합만족도에 영향을 미치는 요인: 서비스품질 만족도, 서비스상품 만족도, 서비스체험 만족도를 중심으로)

  • Kim, Minjeong;Rhee, Hyongjae
    • Journal of Service Research and Studies
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    • v.9 no.3
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    • pp.17-38
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    • 2019
  • With recent dispersion of sharing economy, consumption culture of sharing, instead of ownership, gets wide spread in the age groups of 20-30. Even though the market of car sharing expands rapidly, little research has been done in the area of service quality and consumer behavior regarding car sharing services. Given that future market potential gets larger, it seems highly meaningful to conduct research on customer satisfaction and their usage behavior regarding car sharing services. This paper intends to analyze main factors affecting overall customer satisfaction on car sharing services; satisfaction on service quality, satisfaction on service product, and satisfaction on service experience. Each dimension of the three factors is also analyzed to see if there is any differences between major three brands. Further analysis has been done for checking any effects of gender and usage purpose of customers on the overall customer satisfaction. Key results of the analysis show all the three main factors have significant effect on overall customer satisfaction. Each dimension of service quality has significant effect on satisfaction on service quality. Dimensions of parking lot accessability and vehicle maintenance in service product evaluation, have greater effect on satisfaction on service product. In service experience, positive affect have greater effect than negative affect on satisfaction on service experience. Women users perceive larger overall customer satisfaction on car sharing services than men users. On the types of usage purpose, Users with purpose of tour and dating/meeing friends perceive larger overall customer satisfaction on car sharing services than user without it. Further managerial implications of car sharing services are also provided.

Image Making As a Planning/Design Principle: A Case Study of Andong Municipal Museum Complex (AMMC)

  • Lee, Do Young
    • Architectural research
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    • v.3 no.1
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    • pp.21-27
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    • 2001
  • This study addressing the underlying strategies for Andong municipal museum complex development is in timely view that Andong has obtained a worldwide reputation as a treasury of traditional Korean Confucian culture. Thus far, there has been a tendency that various local museums are proposed to meet architectural aspirations architects and users commonly hold. Overall, though, the major role they play in making overall city image has not been considered in a systematic manner. Based on Lee's (2001) two previous studies, this study summarized the utility of cognitive distance and cognitive map concepts, which are proposed by Kevin Lunch (1976) to evaluate city image, in planning Andong municipal museum complex (AMMC). Sample is stratified into city residents and outsiders, and also into the general public and design-related professionals to see if there is any group difference in constructing their mental image. Three major findings are obtained. First, familiarity, so-called the degree of knowing, is the function of the length of stay in a designated area. That is, the longer people stay in Andong, the more likely they are familiar with its overall environmental aspects. Second, mental proximity of Andong municipal museum complex relative to existing cultural landmarks is closely related to the degree of how people value those landmarks in terms of their significance. Dosan Seowon and Hahoe folk village are most highly valued, which means higher proximity. Third, functional diversity turned out to be the most important design dimension, while display mechanism are least valued. Cognitive simulations of this sort are meaningful in that projected composite image might be a rough first approximation of true public image.

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A Numerical Study on Automobile Interior Environment (자동차 실내 환경에 관한 수치적 연구)

  • Lee, Kum-Bae;Jean, Hee-Ho;Ko, Seok-Bo
    • Proceedings of the SAREK Conference
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    • 2006.06a
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    • pp.325-330
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    • 2006
  • Modern people spend more and more time in an automobile in their daily life. In this life, drivers and passengers should create HVAC environment in the automobile interior which is not only for convenient transportation but also give comfortable feeling with dwelling culture. Also, the latest cars give much importance for the role of HVAC system that controls the environment of the area for passengers more than just basic capability. There are solar radiant heat, surface temperature, refrigeration system(temperature, humidity, air current, velocity), and dust for the factor which have an effect on the HVAC environment in the automobile interior, also these are being carried for the HVAC environment delivered an individual character. In this study, we drew the automobile interior as three dimension and arranged a method of numerical analysis on HVAC environment in the automobile interior displaying air current distribution and temperature distribution through simulation of the automobile interior on the ventilation volume and outlet area. The aim of this study is to develop the estimated method for HVAC environment. in the automobile interior.

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Effect of perceived luxuriousness on brand equity

  • Kang, Ju-Young M.;Kim, Jieun
    • The Research Journal of the Costume Culture
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    • v.24 no.5
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    • pp.697-708
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    • 2016
  • The term used to describe consumer's valuation of a brand is brand equity. One concept that can be managed and may impact valuation of a prestige brand is "luxuriousness." As the concept of "luxuriousness" appears to be a key factor contributing to the equity of a prestige brand, the purpose of this study was to examine how luxuriousness is related to the brand equity utilizing a model developed by Yoo, Donthu, and Lee (2000). Yoo et al. (2000) identified three dimensions [brand association with awareness (BA), perceived quality (PQ), brand loyalty (BL)] accounted for the equity (OBE) of a brand. We speculated that the five dimensions of luxuriousness (i.e., quality, extended self, hedonism, accessibility, and tradition) would selectively influence the two dimensions of brand equity (BA, PQ) and that loyalty would mediate the relationship between the two dimensions of brand equity (BA, PQ) and overall brand equity. A total of 502 participants aged from 18 to 74 were surveyed in USA. Using AMOS 18, the path analysis was conducted with the maximum-likelihood estimation procedure. The model exhibited a good fit with the data and all hypotheses were supported except one. Quality, accessibility, and hedonism dimensions of luxuriousness affected perceived quality of the equity of a brand. Hedonism and extended self dimensions affected brand association with awareness. However, tradition dimension did not significantly influence brand association with awareness. Overall, this research expands understanding of brand equity as it documents the contributions of luxuriousness, a component that can be controlled by brand managers.

Digital Home Network Product Design Process & the Design Proposition (디지털 홈 네트워크 제품디자인 프로세스와 디자인 제안)

  • Lee, Dae-Woo
    • The Journal of the Korea Contents Association
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    • v.9 no.5
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    • pp.118-127
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    • 2009
  • The current global spread of communal residential culture and the trend of continuing increase of newly built apartment environment are increasing the demand of home network products for each residential space and among households. The continuing advancement of ubiquitous and the introduction and development of home network system are promoting the demand for the product design. The residential culture change and the apartment interior design change are having mutual effects on the lifestyle of residents and the product design and demand new product design. Coordination with interior products, interior style and products, product and fashion design and reconstruction and fusion between the products and their element factors are the core element of this design study. Based on this, integrated design strategy and concept research and analysis were studied for the design process, and have been expressed in three dimension design through which the design functionality and the external aesthetical design can correspond to each other. It's because the aspect of the future development prospect can be predicted in detail by presenting practical design plan.

A Study of Older Consumers' Seeking Benefits and Purchase Intentions for and Their Attitudes towards Functional Materials (노년층 소비자의 기능성 소재 추구 혜택, 태도 및 구매 의도에 관한 연구)

  • Hong, Kyung-Hee;Choi, In-Ryu
    • The Research Journal of the Costume Culture
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    • v.17 no.1
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    • pp.149-161
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    • 2009
  • The purposes of this study are to examine older consumers' seeking benefits and purchase intentions for functional materials and their attitudes towards such materials and investigate the differences between purchasers and non-purchasers of functional materials in their seeking benefits for functional materials and their emphasis on self development and pursuit of fashion. In doing so, this study samples the older adult population living in Seoul, Busan, and Gyeonggi Province and analyzes 267 questionnaire responses obtained from the sampled older consumers. The main findings from the analysis are as follows: First, the older consumers' seeking benefits for functional materials were represented by three different dimensions: reliability, brands/fashion, and others-oriented pursuit. Second, the influence on the dimension of reliability was more significant in the order of knowledge on functional materials, pursuit of fashion, and emphasis on self-development the interest in garments and the pursuit of fashion were found to have significant influence on the dimensions of brands/fashion and others-oriented pursuit. Third, the respondents' attitudes towards functional materials showed significant correlations with the dimensions of reliability and others-oriented pursuit, while their purchase intentions for functional materials were significantly influenced by the dimensions of reliability and brands/fashion. Finally, when the differences in the respondents' seeking benefits for functional materials and their pursuit of fashion and emphasis on self-development were investigated according to whether or not they had ever purchased clothes made from any functional material, the group of purchasers showed a higher mean value than the non-purchaser group.

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Assessing how the Yonsei University Foodservice is perceived by the students: Toward an effective strategy formulation (효율적인 대학급식 관리체계 및 경영전략을 위한 소비자 태도 분석)

  • Yang, Il-Sun;Jang, Yoon-Jung;Kim, Sung-Hye;Kim, Dong-Hoon
    • Journal of the Korean Society of Food Culture
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    • v.10 no.4
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    • pp.327-337
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    • 1995
  • The purposes of this study were to: (a) identify college students' patronage behaviors, (b) develop an instrument measuring the attitudes of University Students towards university foodservices management practices, (c) determine university students' attitude towards the four types of university foodservices, and (d) provide recommendations on marketing strategies for university foodservice. Questionnaires were hand delivered to 600 Yonsei University students by designated coordinators. A total of 549 questionnaires were usable; resulting in an 93.3% response rate. The survey was conducted between November 28 to December 4, 1995. Statistical data analysis was completed using the SAS Programs for descriptive analysis, T-test, ${\chi}^2$ test, ANOVA, Factor Analysis and Stepwise Multiple Regression. Most (88.3%) of students were patronizing university foodservices for lunch. Underground student foodservice (40.1%) and Restaurants outside the campus (33.7%) were primarily used for lunch and dinner respectively. Eighty six percent of university students had 1 to 2 meals per day at university foodservices. The reasons given by students for patronizing university foodservices were as follows: location, time, price, menu, taste. Most of the respondents were least satisfied with hygiene, taste, menu and atmosphere. Data indicated strong support for eight priori dimensions in terms of food, menu, atmosphere, hygiene, employee attitude, facilities and convenience. After the factor analysis, price, fast service and foodservice location attributes were rearranged, combined and created a new dimension called as 'access'. Three dimensions in terms of menu, hygiene, convenience were important to students although performance was perceived as poor through importance-performance analysis. Most of students were not satisfied with all four types of university foodservices. In terms of food quality and price which university foodservices offer, most of respondents were moderately satisfied. According to multiple regression analysis, 93.31% of the variance respondents' satisfaction score could be explained by food, menu, price, atmosphere, hygiene, employee attitude, facilities, and convenience dimensions.

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