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Reduction effects of N-acetyl-L-cysteine, L-glutathione, and indole-3-acetic acid on phytotoxicity generated by methyl bromide fumigation- in a model plant Arabidopsis thaliana (모델식물 애기장대에 대한 훈증제 메틸브로마이드의 약해발생 및 N-acetyl-L-cysteine, L-glutathione, indole-3-acetic acid의 약해억제 효과)

  • Kim, Kyeongnam;Kim, Chaeeun;Park, Jungeun;Yoo, Jinsung;Kim, Woosung;Jeon, Hwang-Ju;Kim, Jun-Ran;Lee, Sung-Eun
    • Korean Journal of Environmental Biology
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    • v.39 no.3
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    • pp.354-361
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    • 2021
  • Understanding the phytotoxic mechanism of methyl bromide (MB), an essential fumigant during the quarantine and pre-shipment process, is urgently needed to ensure its proper use and reduce international economic losses. In a previous study, two main MB-induced toxic mechanisms such as reactive oxygen species (ROS) and auxin distribution were selected by analyzing transcriptomic analysis. In the study, a 3-week-old A. thaliana was supplied with 1 mM ROS scavengers [N-acetyl-L-cysteine (NAC) or L-glutathione (GSH)] and 1µM indole-3-acetic acid(IAA) three times every 12 h, and visual and gene expression assessments were performed to evaluate the reduction in phytotoxicity by supplements. Phytotoxic effects on the MB-4h exposed group were decreased with GSH application compared to the other single supplements and a combination of supplements at 7 days post fumigation. Among these supplements, GSH at a concentration of 1, 2, and 5mM was suppled to A. thaliana with MB-fumigation. During a long-term observation of 2 weeks after the fumigation, 5 mM GSH application was the most effective in minimizing MB-induced phytotoxic effects with up-regulation of HSP70 expression and increase in main stem length. These results indicated that ROS was a main key factor of MB-induced phytotoxicity and that GSH can be used as a supplement to reduce the phytotoxicity of MB.

Development of High-frequency Data-based Inflow Water Temperature Prediction Model and Prediction of Changesin Stratification Strength of Daecheong Reservoir Due to Climate Change (고빈도 자료기반 유입 수온 예측모델 개발 및 기후변화에 따른 대청호 성층강도 변화 예측)

  • Han, Jongsu;Kim, Sungjin;Kim, Dongmin;Lee, Sawoo;Hwang, Sangchul;Kim, Jiwon;Chung, Sewoong
    • Journal of Environmental Impact Assessment
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    • v.30 no.5
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    • pp.271-296
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    • 2021
  • Since the thermal stratification in a reservoir inhibits the vertical mixing of the upper and lower layers and causes the formation of a hypoxia layer and the enhancement of nutrients release from the sediment, changes in the stratification structure of the reservoir according to future climate change are very important in terms of water quality and aquatic ecology management. This study was aimed to develop a data-driven inflow water temperature prediction model for Daecheong Reservoir (DR), and to predict future inflow water temperature and the stratification structure of DR considering future climate scenarios of Representative Concentration Pathways (RCP). The random forest (RF)regression model (NSE 0.97, RMSE 1.86℃, MAPE 9.45%) developed to predict the inflow temperature of DR adequately reproduced the statistics and variability of the observed water temperature. Future meteorological data for each RCP scenario predicted by the regional climate model (HadGEM3-RA) was input into RF model to predict the inflow water temperature, and a three-dimensional hydrodynamic model (AEM3D) was used to predict the change in the future (2018~2037, 2038~2057, 2058~2077, 2078~2097) stratification structure of DR due to climate change. As a result, the rates of increase in air temperature and inflow water temperature was 0.14~0.48℃/10year and 0.21~0.41℃/10year,respectively. As a result of seasonal analysis, in all scenarios except spring and winter in the RCP 2.6, the increase in inflow water temperature was statistically significant, and the increase rate was higher as the carbon reduction effort was weaker. The increase rate of the surface water temperature of the reservoir was in the range of 0.04~0.38℃/10year, and the stratification period was gradually increased in all scenarios. In particular, when the RCP 8.5 scenario is applied, the number of stratification days is expected to increase by about 24 days. These results were consistent with the results of previous studies that climate change strengthens the stratification intensity of lakes and reservoirs and prolonged the stratification period, and suggested that prolonged water temperature stratification could cause changes in the aquatic ecosystem, such as spatial expansion of the low-oxygen layer, an increase in sediment nutrient release, and changed in the dominant species of algae in the water body.

Effects of Initial Shoot, Root Length, and Acclimating Substrates on Survival Rate of Plantlets Regenerated from Somatic Embryos of Larix kaempferi (일본잎갈나무 체세포배 유래 식물체의 초기 신초와 뿌리 길이, 순화용 기질이 생존율에 미치는 영향)

  • Lee, Na Nyum;Yun, A Young;Kim, Ji Ah;Kim, Tae Dong;Kim, Yong Wook;Han, Sim Hee
    • Journal of Korean Society of Forest Science
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    • v.109 no.4
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    • pp.413-420
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    • 2020
  • We analyzed the growth characteristics of each cell line and acclimating substrate of Larix kaempferi plantlets regenerated from somatic embryos, with the goal of increasing the survival rate during the acclimation phase. Somatic embryos from three embryogenic cell lines (L14-66, L16-18, and L17-B4) were used, and the acclimating substrates were commercial soils for Larix species (Larix-Soil) and horticultural corps (Hort-Soil), Elle-pot, and Peat-plug. The average initial shoot and root length was shortest in L14-66 and longest in L17-B4. The average survival rate by cell line was highest (87.0%) in L17-B4 and lowest (64.3%) for L14-66. Survival rates by substrate were highest in Elle-pot (88.5%) and Peat-plug (88.9%). The initial shoot length of the L14-66 plantlets was highly correlated with survival rates in the Larix-Soil (r = 0.852), Hort-Soil (r = 0.692), and Elle-pot (r = 0.867) substrates, but not in Peat-plug with high total nitrogen content. The initial shoot length of the L17-B4 plantlets was not correlated with the survival rate in any of the substrates. The initial root length of the L14-66 plantlets was highly related to survival rates in the Larix-Soil (r = 0.986), Elle-pot (r = 0.846), and Peat-plug (r = 0.802) substrates, and the survival rate of the plantlets was higher as the initial root length was longer. The initial root length of the L17-B4 plantlets was related to survival rate only in the Larix-Soil (r = 0.896) and Elle-pot (r = 0.696) substrates. In conclusion, to increase the survival rate of plantlets, root length should be considered over shoot length, and it is recommended to use substrates with high nitrogen content, such as Peat-plug, or to add fertilizer, during the acclimating process. In addition, in order to increase the survival rate, lines with high initial growth should be developed.

A Study on Type Classification of Erosion Control Dam using Ecosystem Connectivity (생태연결성을 고려한 사방댐 유형분류에 관한 연구)

  • Koo, Gil-Bon;Kim, Min-Sik;Kim, Chul;Yu, Seung-mun
    • Journal of Korean Society of Forest Science
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    • v.100 no.3
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    • pp.483-493
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    • 2011
  • Erosion control dams play a primary role in preventing or controlling natural disasters (landslide and debris flow etc.) and also conserve ecosystem in forested watersheds. This study examines structural characteristics of the dams such as the height of ecosystem control and the ecosystem permeability of the erosion control dams under standard drawings and the existing construction works. The objective of this study was to characterize the type classification of erosion control dams as ecosystem. Average permeability was highest on eco-piller dam (63.0%), followed in increasing order by wire rope (13.9%), silt dam (10.9%), multifunctional dam (7.2%), and gravity dam (0.4%). The height of ecosystem control was highest on gravity dam (3.2 m), followed in increasing order by multifunctional dam (1.7 m), wire rope dam (1.2 m), silt dam (0.6 m), and eco-piller dam (0.0 m). Criteria for defining the height of ecosystem control was indefinite. We grouped erosion control dams into three functional types (eco-connection, eco-semi connection, and eco-disconnection) by considering physical and structural characteristics such as the ecosystem permeability and the height of ecosystem control. The type of eco-connection (permeability > 20%) had connection areas from streambed to adjacent riparian areas, and these connection areas serve as ecosystem corridors for fauna and flora. Typical wildlife species includes mammals, reptiles, amphibians, and fishes. The type of eco-semi connection (5% < permeability < 20%) had < 2 m in the eco-barrier height from streambed, however, this type of dams partially serve as wildlife corridors and often provide fish ways. The type of eco-disconnection (permeability < 5%) had > 2 m in the eco-barrier height from streambed, thereby preventing wildlife movement.

Characteristics of Growth and Seedling Quality of 1-Year-Old Container Seedlings of Quercus myrsinaefolia by Shading and Fertilizing Treatment (피음 및 시비처리에 따른 가시나무 1년생 용기묘의 생장과 묘목품질 특성)

  • Sung, Hwan In;Song, Ki Sun;Cha, Young Geun;Kim, Jong Jin
    • Journal of Korean Society of Forest Science
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    • v.100 no.4
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    • pp.598-608
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    • 2011
  • The purpose of this study was to examine potential effects of shading and fertilizing treatment - two basic applicable factors in production of 1-year-old container seedling - on growth and seedling quality of Quercus myrsinaefolia, one of evergreen tree species in warm temperate zone of Korean. Every experimental process was conducted in a facility that consisted of compartments under the lighting control with full sunlight and shading (35%, 55% and 75% of full sunlight). Based on fertilizing treatment, this study made an experiment in 4 groups of container seedling: control (non-treated seedlings) and 1000, 2000, 3000 ppm group (3 groups with different concentrations of water-soluble fertilizer (N:P:K=19:19:19, v/v). Seedlings under 55% shading with 2000 ppm concentration showed the highest height (totaling 21.1 cm), and under 35% shading with 2000 ppm concentration showed the highest root collar diameter growth (totaling 3.96 mm) among others. All three fertilizing groups except control showed H/D ratio ranging from 4.27 to 5.26 regardless of fertilizer concentration under 35% and 55% shading where container seedlings showed a tendency toward excellent growth of height and root collar diameter. Overall, 2000 ppm group under 55% shading showed highest dry mass production of leaves (1,292 g) among others, while 2000 ppm group under 35% shading showed highest dry mass production of shoots and roots (0.592 g and 0.998 g, respectively) among others. Also, it was found that 2000 ppm group under 35% shading showed the highest dry mass production of whole seedling, which was followed by 2000 ppm group under 55% shading and 3000 ppm group under 35% shading, respectively. According to analysis on LWR of Quercus myrsinaefolia depending on shading and fertilizing treatment, it was found that 3000 ppm group under 75% shading showed highest LWR level among others, whereas every fertilized group showed lower RWR level than control without fertilizing regardless of shading levels. In general, all fertilized groups under 55% shading had relatively high quality index (QI), and 2000 ppm group under 35% shading had highest QI among others. Based on the findings of this study, it is concluded that shading level ranging from 35 to 55% and fertilizing concentration of 2,000 ppm are suitable for producing 1-year-old container seedlings of Quercus myrsinaefolia with excellent growth and high quality index.

A Comparative Study on the Information of Zooplankton Community Based on Towing Type and Depth in the Lake Ecosystems (정수생태계 동물플랑크톤 채집 시 네트 인양 유형 및 수심에 따른 군집 정보 비교)

  • OH, Hye-Ji;Chae, Yeon-Ji;Ku, Doyeong;Kim, Yu-Jin;Wang, Jeong-Hyeon;Choi, Bohyung;Ji, Chang Woo;Kwak, Ihn-Sil;Park, Young-Seuk;Nam, Gui-Sook;Kim, Yong-Jae;Chang, Kwang-Hyeon
    • Korean Journal of Ecology and Environment
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    • v.53 no.4
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    • pp.365-373
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    • 2020
  • Biomonitoring Survey and Assessment Manual for lake ecosystem suggest zooplankton collection methods to compare relatively the number of species, population density, and community indices, taking into account the convenience of the field sampling according to the sites' water depth. In this study, the oblique towing and 20 m vertical towing methods presented in the manual were respectively compared with the whole water column-vertical towing and we analyzed the differences and characteristics of zooplankton community information gathered by each collection method. For community indices, there was no difference in the comparison of oblique/vertical towing methods in the shallow lake, but in the deep lake, the diversity and richness indices increased when vertically towing through whole water column rather than when limiting the towing depth to 20 m. In addition, the total zooplankton density collected by the oblique/20 m vertical towing methods was about three times higher than the whole water column-vertical towing method, which means that the density of zooplankton community can be overestimated depending on the collection methods. It appears to be results of differences in the zooplankton density by water layer arising from their vertical distribution and in filtered raw water quantity according to the towing depth/distance. Hence, for zooplankton community information to be used as a functional quantitative indicator representing the entire lake, it would be more appropriate to apply the whole water column-vertical towing method with considering the distribution of zooplankton density by depth and contribution rate of each water layer when converting total zooplankton density.

A rudimentary review of the ancient Saka Kurgan burial rituals - Focused on the case of Katartobe Ancient Tombs in the Zhetisu Region - (고대 사카 쿠르간 매장의례의 초보적 검토 - 제티수지역 카타르토베 유적 사례를 중심으로 -)

  • NAM, Sangwon;KIM, Younghyun;SEO, Gangmin;JEONG, Jongwon
    • Korean Journal of Heritage: History & Science
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    • v.55 no.1
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    • pp.63-84
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    • 2022
  • One of the ancient nomadic cultures, the Saka is generally regarded as an important intermediary in the ancient Eurasian cultural network. This study is the reinterpretation of the excavations conducted on the Katartobe tombs site of the Saka culture through a joint three-year-long project by the National Research Institute of Cultural Heritage in Korea in collaboration with the Cultural Heritage Research Institute under the National Museum of the Republic of Kazakhstan. The main discussion of the study deals with the burial rituals performed by the community who built the Katartobe tombs by the comparison and review of the various researches on the Saka tombs based on the archaeological artifacts discovered during excavation. The research has shown that the Saka tribes maintained the tradition of burying domesticated animals, such as horses, with its owner and performed burial rituals which often involved the use of fire. The archaeological remains of the Saka also show that the burial rituals like these formed the key aspect of their cultural heritage. The archaeological discoveries also show that the Saka mourners built wooden cists under a single mound when they needed to bury multiple corpses at once and sustained the practice of excarnation when burying the bodies of those who died in the different periods of time. Some burials included a tomb passage which was used not only for carrying the deceased but also for a separate burial ritual. The main discussion of this study also deals with the remnants of bones of animals buried with their deceased owners in the same kurgan, as well as the animal species and their locations in the kurgan, resulting in the discovery of diverse meanings connected with them. The pottery buried in the tombs were largely ceremonial offering vessels, just like others excavated at nearby Saka tombs and located around the buried corpse's head facing toward the west. The excavation of the tombs also shows that two vessels were arranged at the corners of the coffin where the feet are located, revealing the characteristic features of the burial practices maintained by the tribe who built the Katartobe tombs. It may be too early to come to a definite conclusion on the burial practices of the Saka due to the relative lack of research on the kurgans across Central Asia. Excavations so far show that the kurgans clustered in a single archaeological site tend to display differences as well as uniformities. In conclusion, the ancient Central Asian tombs need more detailed surveys and researches to be able to make strides in an effort to restore the cultural heritage of the ancient Central Asian tribes who played a crucial role in the Eurasian cultural landscape.

Evaluation of Growth Characteristics and Heavy Metal Absorption Capacity of Festuca ovina var. coreana in Heavy Metal-Treated Soils (중금속 처리한 토양에서 참김의털의 생육특성과 중금속 흡수능력 평가)

  • Keum Chul, Yang
    • Ecology and Resilient Infrastructure
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    • v.9 no.4
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    • pp.259-268
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    • 2022
  • In this study, seeds of Festuca ovina var. coreana growing in waste coal landfills exposed to heavy metal contamination for a long time were collected, and growth characteristics and heavy metal accumulation capacity were evaluated through greenhouse cultivation experiments with germinated seedlings, and was conducted for the applicability of phytoremediation technology. Concentration gradients of arsenic-treated artificial soil were 25, 62.5, 125, and 250 mg/kg, respectively, lead concentrations were 200, 500, 1000, and 2000 mg/kg, and cadmium concentrations were 15, 30, 60, and 100 mg/kg, respectively In the arsenic, lead, and cadmium-treated experimental groups, the number of leaves of F. ovina var. coreana decreased in all compared to the control group except for the lead-treated groups (200, 500, and 1000 mg/kg). Length growth of the shoot part was increased in all of the arsenic treatment groups compared to the control group, but decreased in all of the root parts. In the 1000 and 2000 mg/kg lead treatment groups, lengths increased compared to the control group, but in the other treatments, they were shorter than the control group. In the case of the cadmium treatment group, all of the shoot parts were increased compared to the control group, and all of the root parts were decreased. In the case of arsenic treatment, the biomass was decreased at all parts and all concentrations compared to the control group. The 200, 500, and 1000 mg/kg lead treatments showed larger biomass than the control group in both shoot and root parts. In the cadmium treatment group, the biomass of both shoot and root parts decreased compared to the control group. As the concentration of heavy metal treatment increased, both the number of leaves and the biomass by plant parts tended to decrease, and the length growth of the shoot part tended to increase slightly, but the root part tended to decrease slightly. The arsenic accumulation concentrations of the shoot and root parts of the 62.5 mg/kg arsenic treatment area were 9.4 mg/kg and 253.3 mg/kg, respectively. While the shoot part of the 250 mg/kg arsenic treatment area withered away, the arsenic accumulation concentration in the root part was analyzed to be 859.1 mg/kg, In the 2,000 mg/kg lead treatment area, the shoot and root parts accumulated 10,308.1 and 11,012.0 mg/kg, which were 1.1 times higher than the root parts. At 100 mg/kg cadmium treatment, the shoot and root parts were 176.0 and 287.2 mg/kg, and the root part accumulated 1.6 times higher than the shoot part. As a result of tolerance evaluation of F. ovina var. coreana, multi-tolerance to three heavy metals was confirmed by maintaining growth without dying in all treatment groups of arsenic, lead, and cadmium. Plant extraction (phytoextraction) of F. ovina var. coreana was verified as a species that can be applied up to 2,000 mg/kg of soil lead contamination.

Effect of Soil Strength on Seedling Emergence of Rice and Barnyardgrasses in Direct Dry-Seeding (건답직파에서 토양경도가 벼와 피의 출아에 미치는 영향)

  • Kwon, Yong-Woong;Lee, Byun-Woo;Kim, Do-Soon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.489-495
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    • 1996
  • Seedling emergences of four rice varieties (Dongjinbyeo, Dadajo, Galsaekggarak-sharebyeo, and Italiconaverneco) and three barnyardgrass species(Echjnochloa oryzjcola, E. crus-gali var. crus gali E. crus-gali var. praticola) were evaluated in relation to soil strength. Soil strength was varied by compressing the entire volume of soil with a hydraulic jack so as to be 0.5, 1, 2, 3, 6kg /$\textrm{cm}^2$. Soil strength was measured with a penetrometer (Yamanaka type) and soil covering above the seed was 4cm deep. Experiments were conducted at two air temperature conditions of 17 and $25^{\circ}C$. At a soil strength of up to 2kg/$\textrm{cm}^2$, little or no decrease in seedling emergence occurred in all rice varieties and barnyardgrasses tested. Above that value, seedling emergence decreased progressively as the soil strength increased. The degree of decrease was greatest in Dongjinbyeo and smallest in Dadajo among tested rice varieties, and greatest in Echinochloa oryzicola among barnyardgrasses, being greater in barnyardgrasses than rice. Seedling emergence was delayed almost linearly as the soil strength increased. The delay was greatest in Dongjinbyeo among rice varieties and in Echinochloa oryzicola among barnyardgrasses. Mesocotyl length increased as soil strength increased up to 2 to 3kg / $\textrm{cm}^2$ in Dongjinbyeo and Dadajo in 17$^{\circ}C$ and $25^{\circ}C$, and up to 6kg/$\textrm{cm}^2$ in Galsaekggaraksharebyeo and Italiconaverneco in $25^{\circ}C$. Dongjinbyeo showed the least elongation of mesocotyl among rice varieties in any soil strength. The total length of mesotyl, first internode and incomplete leaf showed little variation with soil strength. The total length was longer than the 4cm covering depth in other varieties except Dongjinbyeo. This might have caused the lower emergence rate in Dongjinbyeo than other varieties in higher soil strength.

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Ecological Characteristics of Korean Dark Sleeper, Odontobutis interrupta in Geumdang Stream, Korea (금당천에 서식하는 얼록동사리(Odontobutis interrupta)의 생태 특징)

  • Hwa-Keun Byeon
    • Korean Journal of Environment and Ecology
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    • v.37 no.1
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    • pp.86-93
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    • 2023
  • This study investigated the ecological characteristics of Odontobutis interrupta at the Geumdang Stream from January to December 2021. The riverbed structure of the species habitat was rich in sand and mud. The water was deep, ranging from 21 to 124 cm, with an average of 48 cm. The stream velocity was slow at 0.24 (0.08-0.36) m/sec. The ratio of females to males was 1:0.98, and the total length of collected individuals ranged from 23 mm to 162 mm. The age according to the total length-frequency distribution as of May indicated that the group with a total length of 23-59 mm was one year old, the group with 60-99 mm was two years old, the group with 100-139 mm was three years old, and the group with 140-162 mm was four years old. As a secondary gender characteristic, the females genital papilla had a cylindrical shape, a hollow inside of the tip, and a longer diameter than males. The males had a cone shape with a pointed end. Sexually mature males had the nuptial color, with a black abdomen and whole body. Some females with a length ranging from 60 to 69 mm and all females 70 mm longer were sexually mature. Some males with a length ranging from 70 to 79 mm and all males 80 mm longer were sexually mature. The spawning season was from May to July, and the water temperature was between 19.6℃ to 29℃ during that period. The prosperous spawning season was June (26℃). The average number of eggs in the ovaries was 2,473 (883-4,955) per matured female, and the matured eggs were yellowish and spherical with a mean diameter of 1.42 (1.20-0.54) mm. The correlation between total length and weight was BW=0.0000006TL3.21 with the constant a as 0.0000006 and parameter b as 3.21. The condition factor (K) was 1.67 (1.18-2.43) on average, and the slope was 0.116.