• Journal of Life Science
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• v.27 no.12
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• pp.1494-1499
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• 2017

Oral infection due to Candida albicans is a widely recognized and frequent cause of superficial infections of the oral mucosa (oral candidiasis). Although oral candidiasis is not a life-threatening fungemia, it can cause severe problems in individuals under certain conditions. MicroRNAs (miRNAs) are noncoding, small RNA molecules, which regulate the expression of other genes by inhibiting the translation of target mRNAs. The present study was designed to identify miRNAs in C. albicans and determine their possible roles in this organism. miRNA-sized small RNAs (msRNAs) were cloned in C. albicans by deep sequencing, and their secondary structures were analyzed. All the cloned msRNAs satisfied conditions required to qualify them as miRNAs. Bioinformatics analysis revealed that two of the most highly expressed C. albicans msRNAs, Ca-363 and Ca-2019, were located in the 3' untranslated region of the corticosteroid-binding protein 1 (CBP1) gene in a reverse orientation. miRNA mimics were transformed into C. albicans to investigate their RNA-inhibitory functions. RNA oligonucleotide-transformed C. albicans was then observed by fluorescent microscopy. Quantitative PCR analysis showed that these msRNAs did not inhibit CBP1 gene expression 4 hr and 8 hr after ectopic miRNA transformation. These results suggest that msRNAs in C. albicans possess an miRNA-triggered RNA interference gene-silencing function, which is distinct from that exhibited by other eukaryotic systems.

• The Korean Journal of Elementary Counseling
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• v.4 no.1
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• pp.237-262
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• 2005

The purpose of this study was to examine the effects of an empathy training program on the empathy and bullying's degree for bullies. The research hypotheses set to achieve this goal were as follows. 1. An experimental group that gets empathy training might make a better improvement in empathy immediately after the training than a control group that doesn't, and the effect of the training might last till eight weeks later. 2. An experimental group that undergoes empathy training might show a more significant decrease in bullying's degree for bullies immediately after the training than a control group that doesn't, and the effect of the training might last till eight weeks later. The subjects in this study were 20 children who were designated as bullies when 40 fifth graders from Y Elementary school and 31 children from S Elementary school in the city of T, south Gyeongsang province, took K-PNI. Those children were asked to tell about who were attackers and who were victims, and 10 children each were selected from the two elementary schools as bullies, who were respectively selected as an experimental group and a control group. The empathy training program used in this study was prepared by modifying the empathy training programs developed by Shin Gyeong- il(1994). That program was prepared to be appropriate at fifth grader level. To assess how much the selected children bullied their peers, Kim Seok-jin(1999)'s School Bullying Inventory was utilized. Besides, Song Ho-yeon's revised version to assess the change of counselee empathy was employed. In order to analyze the resulte, ANOVA was implemented. The conclusions obtained from the results and discussion of this study are as follows. First, the empathy training program was effective in changing the empathy of the bullies for the better, and their improved empathy remained the same eight weeks later. Second, the empathy training program had an effect in reducing bullying's degree for bullies, and there was no change in their reduced bullying's degree eight weeks later. Third, the empathy training program served to change their neglecting/ ostracizing and ridiculing/teasing behaviors. And the retention test that was implemented eight weeks later showed the effect of training remained unchanged. Fourth, the empathy training program was effective in changing their cursing/threatening actions according to the posttest results, but the retention test showed that its effect didn't keep on. Fifth, the empathy training program didn't bring any changes to their robbing/striking actions either immediately after the training or eight weeks later.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.318-326
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• 2002

The pathogenic marine bacterium Vibrio vulnificus is the causative agent of food-borne diseases such as life-threatening septicemia. To better understand this organism's strategies to survive osmotic stress, a mutant that was more sensitive to high osmolarity was screened from a library of mutants constructed by a random transposon mutagenesis. By a transposon-tagging method, putAP genes encoding a proline dehydrogenase and a proline permease were identified and cloned from V. vulnificus. The amino acid sequences deduced from nucleotide sequences of putAP from V. vulnificus were 38 to $59\%$ similar to those of PutA and PutP reported from other Enterobacteriaceae. Functions of putAP genes were assessed by the construction of mutants, whose putAP genes were inactivated by allelic exchanges. When proline as the sole carbon or nitrogen source was used, the putA mutant was not able to grow to the substantial level, revealing the proline dehydrogenase is the only enzyme for metabolic conversion of proline into other amino acids. Although the growth rate of the putP mutant on proline as the sole carbon or nitrogen source was significantly reduced, the mutant still grew. This indicated that at least one more proline permease is produced by V. vulnificus. The putP mutant decreased approximately $2-log_10$ CFU/ml after a hyperosmotic challenge, while the parent strain decreased approximately $l-log_10$ CFU/ml. This result suggests that the gene product of putP contributes to the osmotic tolerance of V. vulnificus.

• Journal of Navigation and Port Research
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• v.39 no.4
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• pp.285-291
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• 2015

Exposure to cold sea water can be life-threatening to the drowned individual. Although appropriate life jacket can be usually be provided for the buoyance at the drowning accident, heat loss can make the drowned individual experience the hypothermia. Inflatable life jackets filled with inflatable air pocket can increase the thermal protection as well as the buoyancy force. Because it is important to know how the human body behaves unde the different life jacket, present study compares the thermal insulation capacity of solid type life jacket with that of inflatable life jacket. In order to represent the insulation capacity of life jacket, thermal resistance is estimated based on the assumption of steady-state. Also, a transient three-dimensional thermal distribution of the thigh is analyzed by using finite element method implementing the Pennes bioheat equation. The finite element model is a segmental, multi-layered representation of the body section which considers the heat conduction within tissue, bone, fat and local blood flow rate.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.36 no.1
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• pp.89-96
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• 2018

JejuHaenyeo(women diver) culture has been designated as UNESCO Intangible Cultural Heritage of Humanity(ICH) in 2016. However, the number of new Haenyeo is decreasing due to the hardships and difficulties of Haenyeo job, and the aging factor of Haenyeo is threatening the cultural preservation and inheritance. The study aims to analyze JejuHaenyeo's awareness on self, community and UNESCO ICH designation. The study further aims to check any significant difference in Haenyeo's self-awareness per age group to conclude an effective strategy for permanence of Haenyeo culture. 228 JejuHaenyeo were interviewed at "The 10th JejuHaenyeo World Festival" around the Jeju Haenyeo Museum in Gujwa-eup, Jeju-si. The test result shows the difference per age group on awareness on self and UNESCO ICH designation status. The younger the generation of Haenyeo it gets, the awareness on self and UNESCO ICH designation status of Haenyeo gets lowerer. Without an immediate improvement to supplement JejuHaenyeo's awareness on self and UNESCO ICH designation status, preservation and inheritance of JejuHaenyeo culture may not be an easy task in the future.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7812-7819
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• 2015

Nursing students experienced anxiety and fear of building relationships with psychiatric patients due to lack of professional knowledge about metal illness and skill to take care of the patients. The simulation education is known as an effective teaching-learning method since being performed in a non-threatening environment similar to clinical setting. The purpose of this nonequivalent quasi-experimental study was to identify the effect of simulation on anxiety and self-confidence about the psychiatric-mental health nursing practice. The third year nursing students at a three year nursing program were participated in this study. For the experimental group (N=28) the simulation education using the standardized patient based on the scenario for patients with schizophrenia was performed, and for the control group (N=18), the debated education applying the case study same as for the experimental group was made in the class. Using SPSS, t-test and ANCOVA were analyzed to compare the anxiety and self-confidence between the experimental and control groups. As a result of the study, the anxiety score on psychiatric-mental health nursing clinical practice of the experimental group significantly decreased, compared to that of the control group, but the self-confidence score for the performance of psychiatric nursing care showed no significant differences between the groups. Accordingly the simulation education can be an effective educational method to reduce anxiety about psychiatric-mental health nursing clinical practice for nursing students.

• Environmental and Resource Economics Review
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• v.27 no.1
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• pp.139-160
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• 2018

Using survey data of selected 1,349 individuals nationwide in Korea, we measure the influencing factors for the acceptance of nuclear power and estimates the probability of acceptance under several scenarios with different percentages of monetary compensation. Results of panel probit demonstrate that nuclear risk aversion tendency was found to be higher in case of female, younger age, past experience of extreme event such as an earthquake. However, the residents' residency nearby the nuclear power plant was not related to the risk-aversion tendency. In addition, we found that the nuclear acceptance is improved when the monetary compensation rate is increased. Although the policy demand intended to reduce GHG emissions in South Korea, the expansion of nuclear power is not be easy due to the occurrence of recent strong earthquakes because the risk attitude of an individual is influenced by subjective assessments formed through direct and indirect experiences of natural disasters such as an earthquake. Our results suggest that the opposition to construction of nuclear power plant is expected to be further intensified especially when combined with the experiences of threatening earthquakes. As a result, the debate and policy conflicts of nuclear power plants will consistently continue and large social costs are apparent for the acceptance of nuclear power plant.

• Development and Reproduction
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• v.10 no.4
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• pp.247-254
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• 2006

It is well publicized that the existence of various endocrine disrupting chemicals threatens normal sexual development of many sedentary marine fishes in the coastal areas. However, a suitable marine fish species for efficient monitoring of this threatening has yet to be identified. One of the difficulties in estimating the effect of endocrine disruption in marine fish is the absence of clear distinction between testicular and ovarian structures at the early stages of sex differentiation. In search of a potential test species, we have investigated the microscopic structures of sexually undifferentiated and differentiated gonads and the susceptibility of gonadal differentiation to exogenous sex steroids during the sex differentiation period in a sedentary marine rockfish, Sebastes schlegeli. Male gonads in this species contained dark pigmentation that made them distinct from female gonads. Treatment either with $estradiol-17\;{\beta}(E_2)$ or $17\;{\alpha}-methyltestosterone$ (MT) significantly altered the sex ratios with the complete sex changes or the occurrence of ovotestis that was easily identified by the mixed structure of dimorphic gonads (coexistence of ovarian cavity/primary oocytes and dark pigmentation/seminiferous tubules). Results in this study suggest that S. schlegeli can be developed as a monitoring/test fish species for endocrine disruption in marine fish in the coastal areas.

• Journal of the Society of Disaster Information
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• v.8 no.2
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• pp.158-170
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• 2012

Citizens'income has been increased along with the trend of rapid changes in society, and the quality of their lives has been improved as well. As much as the degree of increase of quality of life, the number of spectators for performing art events, etc. who desire to fulfill their needs for enjoyment of varied cultural performances have been increasing, and also a large number of spectators enjoys a variety of festivals being held in each provincial area as well as international events: we still remember such frantic rooting shown by citizens during 2002 World Cup drawing attention and interest of entire nation. There are always risks of loss of human lives if accidents occur as there sult of close-packed crowd gathered at the same time. Therefore, it is required to prepare adequate security measures in order to prevent various accidents before hand. It is hoped that this research work would be of help for further efficient and systematic security management for the performing arts centers or public theaters encouraging the event organizer and the private security firm and the Korea Private Security Association to exert great effort and investments in further projects for development of security technology. Also, it is required to build a performance culture to consider audience's safety first from the beginning to the end of the event on the basis of efficient security management. Furthermore, spect at or sare required to recognize the fact that safety in the site for performing arts should be guaranteed for everyone's sake, and, to achieve this, they are obliged to be more cooperative with the event organizer and the private security firm, forming a trinity all together, in order not to have safety threatening situations in the site of performing arts events.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.9
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• pp.5865-5874
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• 2015

Large accidents such as the recent to the ringing years Pat disaster they become apparent in the human resources, the cause of the accident is also the site corresponding manual member and management blind spots, are aggregated such as safety frigidity. Big problem is safe frigidity of workplace than accident. It is also a reason to introduce a punitive damages system in offices and business owners that caused the direct safety accident from this point. Punitive damages agent and compensation for mental damage, by so as to compensate for far more money than the actual damages, which carry a punishment nature to prevent a situation where the tort is repeated. Currently, it admits "compensatory damages system" corresponding to the damage that gave basically damage by law, but are subject to criticism that not properly relief victims. However, there is a need for the introduction of punitive damages system for safety accident prevention. This is, this system, while to prevent the recurrence of the reverse safety accident, strive to investment and management for safety, healthy companies can operate in the direction of giving more opportunities, national companies that are ignoring and threatening the safety, but to leave because the match also to social justice.