• BMB Reports
• /
• v.43 no.12
• /
• pp.773-780
• /
• 2010

Cancers are still difficult targets despite recent advances in cancer therapy. Due to the heterogeneity of cancer, a single-treatment modality is insufficient for the complete elimination of cancer cells. Therapeutic strategies from various aspects are needed. Gene therapy has been expected to bring a breakthrough to cancer therapy, but it has not yet been successful. Gene therapy also should be combined with other treatments to enhance multiple therapeutic pathways. In this view, gene delivery vector itself should be equipped with intrinsic anti-cancer activities. HVJ (hemagglutinating virus of Japan; Sendai virus) envelope vector (HVJ-E) was developed to deliver therapeutic molecules. HVJ-E itself possessed anti-tumor activities such as the generation of anti-tumor immunities and the induction of cancer-selective apoptosis. In addition to the intrinsic anti-tumor activities, therapeutic molecules incorporated into HVJ-E enabled to achieve multi-modal therapeutic strategies in cancer treatment. Tumor-targeting HVJ-E was also developed. Thus, HVJ-E will be a novel promising tool for cancer treatment.

• Child Health Nursing Research
• /
• v.8 no.1
• /
• pp.5-19
• /
• 2002

The purpose of this study was to identify therapeutic play need and the degree of the nurses's therapeutic play performances perceived by hospitalized children's mothers, and then to compare the difference between the degree of the need and perceived performances. The subjects of this study consist of 121 hospitalized children's mothers. A questionaire for this study was 29 item likert type 4 point scale developed on the basis of literature review, hospitalized children's mother interview and researcher's clinical experiences. Data was analyzed by Paired t-test, t-test, ANOVA & Duncan's comparison. Results of this study are summarized as follows : 1. The degree of the therapeutic play need was measured to be high (3.20). The degree of perceived therapeutic play need by five components were followed as : physiologic suffering relief & enhancing play (3.39), growth & development facilitating play (3.26), instructional play (3.24), emotional well-being promoting play (3.18) and providing play tool & environment(2.97). 2.The degree of the nurse's therapeutic play performances perceived by mothers was measured to be low (1.60). The degree of perceived performances of the therapeutic play by the five components were followed as : physiologic suffering relief & enhancing play (1.87), instructional play (1.74), emotional well-being promoting play (1.64), providing play tool & environment (1.44) and growth&development facilitating play (1.42). 3. The difference between the degree of the therapeutic play need and nurese's perfor- mances perceived by hospitalized children's mothers were significant statistically (t= 38.54, p=.0001). Also, five components of therapeutic play were significant statistically (p=.0001). Therapeutic play has unique benefits among health care intervention for children. These findings will help in building of the theoretical framework of therapeutic play and enhancing the quality of nursing care for hospitalized children. Therapeutic play program for hospitalized children according to child growth & development and physiologic status are recommended to be developed.

• Journal of Trauma and Injury
• /
• v.34 no.4
• /
• pp.248-256
• /
• 2021

Purpose: Laparoscopy has various advantages over laparotomy in terms of postoperative recovery. The number of surgeons using laparoscopy as a diagnostic and therapeutic tool in abdominal trauma patients is increasing, whereas open conversion is becoming less common. This report summarizes a single surgeon's experience of laparoscopy at a level I trauma center and evaluates the feasibility of laparoscopy as a diagnostic and therapeutic tool for abdominal trauma patients. Methods: In total, 30 abdominal trauma patients underwent laparoscopy by a single surgeon from October 2014 to May 2020. The purpose of laparoscopy was categorized as diagnostic or therapeutic. Patients were classified into three groups by type of surgery: total laparoscopic surgery (TLS), laparoscopy-assisted surgery (LAS), or open conversion (OC). Univariate analysis was performed to determine the advantages and disadvantages. Results: The mechanism of injury was blunt in 19 (63.3%) and penetrating in 11 patients (36.7%). Eleven (36.7%) and 19 patients (63.3%) underwent diagnostic and therapeutic laparoscopy, respectively. The hospital stay was shorter for patients who underwent diagnostic laparoscopy than for those who underwent therapeutic laparoscopy (5.0 days vs. 13.0 days), but no other surgical outcomes differed between the groups. TLS, LAS, and OC were performed in 12 (52.2%), eight (34.8%), and three patients (13.0%), respectively. There was no significant difference in morbidity and mortality among the three groups. Conclusions: Laparoscopic surgery for selected cases of abdominal trauma may be feasible and safe as a diagnostic and therapeutic tool in hemodynamically stable patients due to the low OC rate and the absence of fatal morbidity and mortality.

• Proceedings of the PSK Conference
• /
• 2004.10a
• /
• pp.132-132
• /
• 2004

• Physical Therapy Korea
• /
• v.28 no.4
• /
• pp.235-244
• /
• 2021

Background: The body schema, which is constantly updated using somatosensory information, enables accurate movement. Since pain is reported as a possible source to alter the body schema, the left right judgement test (LRJT) has been widely used in the pain rehabilitation. However, there was a lack of consistency in the effect of the pain on the LRJT results, and for the effect of the LRJT as a part of intervention programs for pain patients. The deeper understand of the LRJT is necessary for better reproducibility, and to expand the therapeutic applications of the LRJT in the pain and musculoskeletal rehabilitation. Objects: This literature review aimed to understand the LRJT and to study the potential of the LRJT for therapeutic applications. Methods: The PubMed database was searched for studies relevant to LRJT. To establish the query set, the term was regarded from various perspectives. Results: The selected studies were classified into three categories: LRJT development, factors influencing LRJT, and therapeutic applications. Conclusion: Left right judgement test is the evaluation tool for the integrity of body schema as well as a tool for implicit motor imagery. Pain, proprioception, and other factors influence the performance of the LRJT.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.6
• /
• pp.2445-2452
• /
• 2012

RNA interference has created a breakthrough in gene silencing technology and there is now much debate on the successful usage of RNAi based methods in treating a number of debilitating diseases. Cancer is often regarded as a result of mutations in genomic DNA resulting in faulty gene expression. The occurrence of cancer can also be influenced by epigenetic irregularities in the chromatin structure which leads to alterations and mutations in DNA resulting in cancer cell formation. A number of therapeutic approaches have been put forth to treat cancer. Anti cancer therapy often involves chemotherapy targeting all the cells in common, whereby both cancer cells as well as normal cells get affected. Hence RNAi technology has potential to be a better therapeutic agent as it is possible to deactivate molecular targets like specific mutant genes. This review highlights the successful use of RNAi inducers against different types of cancer, thereby paving the way for specific therapeutic medicines.

• Molecules and Cells
• /
• v.41 no.8
• /
• pp.717-723
• /
• 2018

Chimeric antigen receptor (CAR) T-cell therapy, an emerging immunotherapy, has demonstrated promising clinical results in hematological malignancies including B-cell malignancies. However, accessibility to this transformative medicine is highly limited due to the complex process of manufacturing, limited options for target antigens, and insufficient anti-tumor responses against solid tumors. Advances in gene-editing technologies, such as the development of Zinc Finger Nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), have provided novel engineering strategies to address these limitations. Development of next-generation CAR-T cells using gene-editing technologies would enhance the therapeutic potential of CAR-T cell treatment for both hematologic and solid tumors. Here we summarize the unmet medical needs of current CAR-T cell therapies and gene-editing strategies to resolve these challenges as well as safety concerns of gene-edited CAR-T therapies.

• Korean Institute of Interior Design Journal
• /
• v.25 no.6
• /
• pp.139-148
• /
• 2016

Chronic patients and staffs in healthcare facilities cannot avoid long-term stays indoors. Therefore, they need to get adequate amounts of sunlight to prevent diseases caused by vitamin D deficiency. This is a case study on the advanced daylighting systems installed to healthcare facilities for therapeutic environments as serving natural light therapy and a pleasant atmosphere. This paper shows daylighting devices that overcome the structural limits of architectural design solutions for inducing natural light. This study presents appropriate daylighting technologies to the purpose and types of healthcare facilities by analyzing therapeutic environmental factors. Natural light delivered through daylighting devices help directly patients recover. In addition, it can improve healthcare providers' performance and productivity, and reduce their errors, thereby creating therapeutic environments for patients, indirectly. Excellent color rendering of solar illumination is also a powerful tool for navigation and wayfinding in the buildings, and for aesthetic treatment in dental office. Furthermore, daylighting systems are streamlined and require the least material and space to attract sunlight up to the deeper inside. But because of expensive cost, they have not yet been widespread. Though daylighting devices are typically more expensive than artificial illuminations to install, the running cost savings can compensate for the initial installation cost. With the development of technology in the future, the market price of them is expected to be formed properly.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2002.10a
• /
• pp.118-120
• /
• 2002

Genetic materials including DNA plasmid are effective delivery vehicle to express interesting gene efficiently and safely not to generate replication competent virus. Moreover, it has advantages to design a better vector and to simplify manufacturing and storage condition. To understand a possible pathogenic mechanism by a flavivirus, West Nile virus (WNV), WNV genome sequence was aligned to other pathogenic viral genome. Interestingly, WNV capsid (Cp) amino acid sequence has some homology to HIV-l Vpr protein. These proteins induce apoptosis in human cell lines as well as in vivo and cell cycle arrest. Therefore, DNA plasmid carrying apoptosis-inducing and cell cycle arresting viral proteins including a HIV-1 Vpr and a WNV Cp protein can be useful for anti-cancer therapeutic applications. This WNV Cp protein is an early expressed protein which can be a reasonable target antigen (Ag) for vaccine design. Immunization of a DNA construct encoding WNV Cp protein induces a strong Ag-specific humoral and Th1-type immune responses in animal. Therefore, DNA plasmid encoding apoptotic viral proteins can be useful tool for therapeutic and prophylactic applications.

• BMB Reports
• /
• v.56 no.2
• /
• pp.71-77
• /
• 2023

Cancers are one of the most dreaded diseases in human history and have been targeted by numerous trials including surgery, chemotherapy, radiation therapy, and anti-cancer drugs. Adult stem cells (ASCs), which can regenerate tissues and repair damage, have emerged as leading therapeutic candidates due to their homing ability toward tumor foci. Stem cells can precisely target malicious tumors, thereby minimizing the toxicity of normal cells and unfavorable side effects. ASCs, such as mesenchymal stem cells (MSCs), neural stem cells (NSCs), and hematopoietic stem cells (HSCs), are powerful tools for delivering therapeutic agents to various primary and metastatic cancers. Engineered ASCs act as a bridge between the tumor sites and tumoricidal reagents, producing therapeutic substances such as exosomes, viruses, and anti-cancer proteins encoded by several suicide genes. This review focuses on various anti-cancer therapies implemented via ASCs and summarizes the recent treatment progress and shortcomings.