• BMB Reports
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• 제50권6호
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• pp.285-298
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• 2017

The cancer stem cell (CSC) hypothesis has captured the attention of many scientists. It is believed that elimination of CSCs could possibly eradicate the whole cancer. CSC surface markers provide molecular targeted therapies for various cancers, using therapeutic antibodies specific for the CSC surface markers. Various CSC surface markers have been identified and published. Interestingly, most of the markers used to identify CSCs are derived from surface markers present on human embryonic stem cells (hESCs) or adult stem cells. In this review, we classify the currently known 40 CSC surface markers into 3 different categories, in terms of their expression in hESCs, adult stem cells, and normal tissue cells. Approximately 73% of current CSC surface markers appear to be present on embryonic or adult stem cells, and they are rarely expressed on normal tissue cells. The remaining CSC surface markers are considerably expressed even in normal tissue cells, and some of them have been extensively validated as CSC surface markers by various research groups. We discuss the significance of the categorized CSC surface markers, and provide insight into why surface markers on hESCs are an attractive source to find novel surface markers on CSCs.

• 대한조선학회논문집
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• 제54권1호
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• pp.26-33
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• 2017

In this study, the surface marker method, one of the particle tracking methods, used to track the free surface is extended to cover the more general cases easily including the collision and separation of the free surface. In surface particle method to redistribute particles effectively using the grid system, the free surface is composed of the sum of quadrilaterals having four curves where fixed markers are placed at ends of each curve. Fixed markers are used to know how curves are connected to each other. The position of fixed markers can move as the free surface deforms but all fixed markers cannot be deleted during all time of simulation to keep informations of curve connection. In the case of the collision or separtion of the free surface where several curves can be intersected disorderly, severe difficulties can occur to define newly states of curve connection. In this study, the adaptable surface parTicle method without fixed markers is introduced. Intersection markers instead of the fixed markers are used to define quadrilaterals. The position of the intersection markers is defined to be the intersection point between the free surface and the edge of the grid and it can be added or deleted during the time of simulation to allow more flexibilities. To verify numerical schemes, two flow cases are simulated and the numerical results are compared with other's one and shown to be valid.

• Journal of Korean Dental Science
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• 제11권2호
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• pp.62-70
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• 2018

Purpose: Bone marrow has long been a source of primary cells. This study was performed to evaluate the effects of age and sex on the cellular viability and expression of stem cell markers of mRNA and on the protein expression of bone marrow stem cells (BMSCs) derived from healthy donors. Materials and Methods: Stem cells were isolated from human bone marrow and plated in culture plates. The shape of the BMSCs was observed under inverted microscope. Quantitative cellular viability was evaluated using a Cell-Counting Kit-8 assay. The expression of stem cell surface markers was tested and a series of quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot was performed to evaluate the expression in each group. Result: The shapes of the cells at 20s, 30s, and 50s were similar to each other. No significant changes in cellular viability were noted among different age groups or sex groups. The BMSCs expressed CD44, CD73, and CD90 surface markers but did not express CD14 and CD34. There were no noticeable differences in CD surface markers among the different age groups. The expressions of CD surface markers were similar between men and women. No significant differences in the secretion of vascular endothelial growth factors (VEGFs) were noted at Day 3 between different age groups. qRT-PCR regarding the expression showed differences between the age groups. However, Western blot analysis showed a decrease in expression but did not reach statistical significance (P>0.05). Conclusion: This study clearly showed no significant differences in shape, cell viability, expression of stem cell surface markers, or secretion of human VEGF among different age groups. However, western blot analysis showed a tendency of age-related decrease which did not reach statistical significance. Collectively, autologous or allogeneic BMSCs should be meticulously applied to obtain optimal results regarding age and sex.

• 한국신뢰성학회지:신뢰성응용연구
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• 제12권2호
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• pp.91-103
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• 2012

Tubular markers for road safety facilities are used to lead the driver's sight line and separate the lanes on the road. Such tubular markers are usually installed on the road and frequently hit by vehicles, they are accordingly requested to assure the product durability. The traditional evaluation method of tubular markers include only quality tests of the material properties. However, most of consuming agencies in charge of road management at fields have proposed problems on long-term performance of the products hit by vehicles under various weather conditions. Therefore, the objectives of this study are to develop the reliability test methods and equipments to simulate the product failures of tubular markers due to vehicle collision and wheel compression and the delamination and discoloration of reflection sheets attached on the surface of the products under high and low temperatures.

• International Journal of CAD/CAM
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• 제3권1_2호
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• pp.61-66
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• 2003

This paper describes a novel experiment that measures skin movement with respect to the flexional motion of a hand. The study was based on MR images in conjunction with CAD techniques. The MR images of the hand were captured in 3 different postures with surface markers. The surface markers attached to the skin where employed to trace skin movement during the flexional motion of the hand. After reconstructing 3D isosurfaces from the segmented MR images, the global registration was applied to the 3D models based on the particular bone shape of different postures. Skin movement was interpreted by measuring the centers of the surface markers in the registered models.

• Asian-Australasian Journal of Animal Sciences
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• 제26권4호
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• pp.588-595
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• 2013

Mesenchymal stem cells (MSCs) are often known to have a therapeutic potential in the cell-mediated repair for fatal or incurable diseases. In this study, canine umbilical cord MSCs (cUC-MSCs) were isolated from umbilical cord matrix (n = 3) and subjected to proliferative culture for 5 consecutive passages. The cells at each passage were characterized for multipotent MSC properties such as proliferation kinetics, expression patterns of MSC surface markers and self-renewal associated markers, and chondrogenic differentiation. In results, the proliferation of the cells as determined by the cumulative population doubling level was observed at its peak on passage 3 and stopped after passage 5, whereas cell doubling time dramatically increased after passage 4. Expression of MSC surface markers (CD44, CD54, CD61, CD80, CD90 and Flk-1), molecule (HMGA2) and pluripotent markers (sox2, nanog) associated with self-renewal was negatively correlated with the number of passages. However, MSC surface marker (CD105) and pluripotent marker (Oct3/4) decreased with increasing the number of subpassage. cUC-MSCs at passage 1 to 5 underwent chondrogenesis under specific culture conditions, but percentage of chondrogenic differentiation decreased with increasing the number of subpassage. Collectively, the present study suggested that sequential subpassage could affect multipotent properties of cUC-MSCs and needs to be addressed before clinical applications.

• 농촌의학ㆍ지역보건
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• 제17권2호
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• pp.129-136
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• 1992

To evaluate the possible route of intrafamilial transmission among carriers of hepatitis B surface antigen ($HB_sAg$), epidemiologic and serologic data were obtained on 107 household contacts of 35 carriers of HBsAg and on 71 household contacts of 25 controls who were negative for serologic markers of hepatitis B virus. The HBsAg prevalence was 26.5% among the contacts of carriers compared to 0.0% among the contacts of controls. And the combined prevalence for all hepatitis markers was 48.5% among the contacts of carriers compared to 26.0% among the contacts of controls(p<0.05). Especially the offspring of carriers showed significantly higher risk in the combined prevalence for all hepatitis markers (p<0.05). There were no significant relationship between HBV infection and past history like acupuncture, transfusion, operation and tattooed. Factors associated with the risk of intrafamilial transmission of HBV were not found in the sharing of household articles such as razor, towel, drinking glass, nail clippers, toothbrush and tableware.

• 한국HCI학회:학술대회논문집
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• 한국HCI학회 2008년도 학술대회 1부
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• pp.39-44
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• 2008

본 논문에서는 변형한 가능 곡면에 가상의 텍스쳐를 투영하기 위한 프로젝터 기반의 실시간 기하 보정 시스템을 제안한다. 다수의 대응점들의 위치를 추적하기 위하여 사용되는 마커들은 프로젝터의 영상이 투영되더라도 그 흔적이 남아있어서 투영 영상을 훼손한다. 또한 출력 영상의 사전 기하 보정 과정에서 병목 현상이 발생하기 때문에 실시간 기하 보정 시스템의 구현이 어렵다. 따라서 본 논문에서는 마커 인식 과정에서 마커에 의해 투영 영상이 훼손되는 현상을 막기 위해 눈에 보이지 않는 적외선 마커를 사용하며, NVIDIA의 Cg ToolKit을 이용하여 고속의 GPU 연산을 수행함으로서 자연스러운 실시간 보정 영상의 출력이 가능하다. 본 논문의 실험 결과에서는 눈에 보이지 않는 마커가 그려진 종이 위에서 사용자와의 상호 작용이 가능한 디지털 영상을 투영한다. 이를 통해 증강 현실 기반의 다양한 디지털 콘텐츠 제작이 가능하다.

• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1149-1153
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• 2006

In this study, nuclease-resistant RNA aptamers that are specific for Jurkat T leukemia cells were selected by a subtractive systemic evolution of ligands by exponential enrichment (SELEX) method. A randomized nuclease-resistant RNA library was incubated with normal peripheral blood mononuclear cells (PBMC) in each round to preclude RNAs that recognize the common cellular components on the surface of normal and cancer cells. The precluded RNAs were used for the selection of Jurkat T cell-specific aptamers, and the specific RNAs were then gradually enriched from start to the following selections. After 16 rounds of the subtractive SELEX, the selected aptamers were found to preferentially bind to Jurkat T cells, but not to the normal PBMC, evidenced by fluorescence-activated cell sorting analysis. Thus, the subtractive SELEX can be used to identify ligands to cancer-specific biological markers without prior knowledge of the nature of markers. The aptamers could be applied to specific cell sorting, tumor therapy, and diagnosis, and moreover, to find cancer cell-specific markers.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 추계학술대회
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• pp.114-115
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• 2012

본 논문에서는 마커를 이용한 기존의 전처리 기술을 개선하여 최소한의 마커로 왜곡을 교정하고 요소 영상을 자동으로 추출하는 방법에 대해 제안한다. 기존의 마커 방식과 성능은 비슷하지만 더 간단한 계산법을 이용한 방법을 제안하고, 이를 위한 컴퓨터 실험을 수행하고 교정 전, 후의 복원 영상을 비교하였다.