• Journal of Animal Reproduction and Biotechnology
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• v.34 no.3
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• pp.139-147
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• 2019

Stem cells are progenitor cells that are capable of self-renewal and differentiation into various cells. Especially, pluripotent stem cells (PSCs) have in vivo and in vitro differentiation capacity into three germ layers and can proliferate infinitely. The differentiation ability of PSCs can be applied for regenerative medicine and tissue engineering. In domestic animals, their PSCs have a potential for preclinical therapy as well as the production of transgenic animals and agricultural usage such as cultured meat. Among several domestic animals, a pig is considered as an ideal model for biomedical and agricultural purposes mentioned above. In this reason, studies for pig PSCs including embryonic stem cells (ESCs), embryonic germ cells (EGCs) and induced pluripotent stem cells (iPSCs) have been conducted for decades. Therefore, this review will discuss the history of PSCs derived from various origins and recent progress in pig PSC research field.

• Fisheries and Aquatic Sciences
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• v.24 no.2
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• pp.63-77
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• 2021

The aim of this research was to investigate different factors, including cryoprotective agents (CPAs), diluents, dilution ratios, equilibrium times, freezing rates, and thawing methods to optimize cryopreservation protocols for large yellow croaker (Larimichthys crocea). The parameters evaluated were sperm motility, sperm activity index (SAI), survival rate, and DNA damage. Different types of CPAs, such as dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), methanol, and glycerol, were tested for sperm preservation. The highest motility, SAI, and survival rate were observed when EG was used. Different diluents such as Stein's solution, Hank's balanced salt solution, marine fish Ringer's solution, artificial seminal plasma (ASP) of small yellow croaker, and Cortland solution were investigated. The highest post-thaw motility was observed upon using ASP as the diluent. Different concentrations of EG were then mixed with ASP to identify the optimal EG concentration. Experimental results showed that the motility (70.33 ± 1.20%), SAI (5), and survival rate (78.30 ± 0.42%) of post-thaw sperm were optimum when 10% EG and ASP were used as the CPA and diluent of cryopreservation, respectively. Post-thaw sperm motility was high at equilibration times below 150 s and at an optimum dilution ratio of 1:1 (sperm: CPA + diluent) and was not significantly different compared with fresh sperm motility. The freezing rate was found to be slow below -10℃/min. The thawing temperature of 45℃ was identified as ideal. The percentage of tail DNA in post-thaw sperm at 10% EG and ASP was also investigated and was found to have more significant DNA damage than that in fresh sperm but significantly lower damage than that in post-thaw sperm at EG concentrations of 5%, 15%, and 20% (p < 0.05). The cryopreservation protocols obtained in this study will be useful in large yellow croaker hatcheries.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7395-7399
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• 2014

Background: Persistent human papillomavirus (HPV) infection, especially with high-risk types such as HPV16 and HPV18, has been identified as the primary cause of cervical cancer. E6 and E7 are the major onco-proteins of high-risk HPVs, which are consistently expressed in HPV infected tissues but absent in normal tissues and represent ideal therapeutic targets for immunotherapy of cervical cancer. Materials and Methods: In this study, the optimized fusion gene HPV18 E6E7 (HPV18 ofE6E7) was constructed according to genetic codon usage for human genes. At the same time, for safety future clinical application, a mutant of HPV18 ofE6E7 fusion gene was generated by site-directed mutagenesis at L52G for the E6 protein and C98G for the E7 protein. Results: HPV18-E6E7 mutant (HPV18 ofmE6E7) constructed in this work not only lost the transformation capability for NIH 3T3 cells and tumorigenicity in BALB/c nude mice, but also maintained very good stability and antigenicity. Conclusion: These results suggest that the mutant should undergo further study for application as a safe antigenspecific therapeutic vaccine for HPV18-associated tumors.

• Journal of Microbiology and Biotechnology
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• v.28 no.6
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• pp.1022-1029
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• 2018

Tuberculosis (TB) remains a serious health issue around the word. Adenovirus (Ad)-based vaccine and modified vaccinia virus Ankara (MVA)-based vaccine have emerged as two of the most promising immunization candidates over the past few years. However, the performance of the homologous and heterologous prime-boost immunization regimens of these two viral vector-based vaccines remains unclear. In the present study, we constructed recombinant Ad and MVA expressing an Ag85B-TB10.4 fusion protein (AdH4 and MVAH4) and evaluated the impact of their different immunization regimens on the humoral and cellular immune responses. We found that the viral vector-based vaccines could generate significantly higher levels of antigen-specific antibodies, $IFN-{\gamma}$-producing splenocytes, $CD69^+CD8^+$ T cells, and $IFN-{\gamma}$ secretion when compared with bacillus Calmette-$Gu{\acute{e}}rin$ (BCG) in a mouse model. AdH4-containing immunization regimens (AdH4-AdH4, AdH4-MVAH4, and MVAH4-AdH4) induced significantly stronger antibody responses, much more $IFN-{\gamma}$-producing splenocytes and $CD69^+CD8^+$ T cells, and higher levels of $IFN-{\gamma}$ secretion when compared with the MVAH4-MVAH4 immunization regimen. The number of $IFN-{\gamma}$-producing splenocytes sensitive to $CD8^+$ T-cell restricted peptides of Ag85B (9-1p and 9-2p) and Th1-related cytokines ($IFN-{\gamma}$ and $TNF-{\alpha}$) in the AdH4-MVAH4 heterologous prime-boost regimen immunization group was significantly higher than that in the other viral vector-based vaccine- and BCG-immunized groups, respectively. These results indicate that an immunization regimen involving AdH4 may have a higher capacity to induce humoral and cellular immune responses against TB in mice than that by regimens containing BCG or MVAH4 alone, and the AdH4-MVAH4 prime-boost regimen may generate an ideal protective effect.

• Development and Reproduction
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• v.11 no.2
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• pp.67-77
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• 2007

Replacement of insulin-producing cells represents an almost ideal treatment for patients with diabetes mellitus type 1. Transplantation of pancreatic islets of Langerhans is limited by the lack of donor organs. Therefore, generation of insulin-producing cells from human embryonic stem cells represents an attractive alternative. The present review summarizes the current knowledge on the differentiation of insulin-producing cells from human embryonic stem cells and their application to the cell therapy for treating diabetes mellitus.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.40
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• pp.32.1-32.8
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• 2018

Background: The free vascularized fibula flap presents many advantages such as sufficient length of the bony segment, good vascularization, better quality of the bone, and a long vascular pedicle, but it is also associated with some disadvantages with regard to prosthetic rehabilitation because of its limited height. Improvement in bone height is necessary for ideal dental implant treatment of reconstructed mandibles. Case presentation: For two squamous cell carcinoma patients, mandibular bone reconstruction was performed secondarily with the peroneal flap after tumor resection. Since the bone height was insufficient at the time of implant treatment, occlusion reconstruction by dental implant was performed after vertical distraction osteogenesis. Conclusions: Vertical distraction osteogenesis is a suitable treatment option for alveolar ridge deficiency resulting from fibula transplantation for mandibular reconstruction following tumor surgery.

• Structural Engineering and Mechanics
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• v.62 no.5
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• pp.567-576
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• 2017

Structural failures are undesirable events that devastate the construction industry resulting in loss of life, injury, huge property loss, and also affect the economy of the region. Roof truss failures occur mainly due to excessive loading, improper fabrication, deterioration, inadequate repair, etc. Although very rare, a roof truss may even fail due to inappropriate location of supports. One such case was reported from the recent failure of a steel roof truss used in an indoor stadium at Kargil in India. Kargil region, being mountainous in nature, receives heavy snowfall and hence the steel roof trusses are designed for heavy snow loads. Due to inappropriate support location, the indoor stadium's steel roof truss had failed under heavy snow load for which it was designed and became an interesting structural engineering problem. The failure observed was primarily in terms of yielding of the bottom chord under the supports, leading to partial collapse of the roof truss. This paper summarizes the results of laboratory tests and analytical studies that focused on the validation of the proposed remedial measure for rehabilitating this distressed steel roof truss. The study presents the evaluation of (i) significant reduction in strength and stiffness of the distressed truss resulting in its failure, (ii) desired recovery in both strength and stiffness of the rectified truss contributed by the proposed remedial measure. Three types of models i.e., ideal truss model, as build truss model and rectified truss model were fabricated and tested under monotonic loading. The structural configuration and support condition varied in all the three models to represent the ideal truss, distressed truss and the rectified truss. To verify the accuracy of the experimental results, an analytical study was carried out and the results of this analytical study are compared with the experimental ones.

• Journal of the Korean Society of Costume
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• v.58 no.2
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• pp.134-148
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• 2008

Methods of delivering products to consumers do not act as less important factor than creative activities to create new product designs. Mobilizing various psychological elements based on human instinct and desire, fashion industry presents a product as an object of envy, gaining viewers' attention. Here, the viewer does not simply take the product as an object to view, but also imagine transformation it will bring. The study of the cause and effects of the interaction, which is found in the relationship between "the viewer" and "what is viewed" is an important factor that needs to be identified in the phase of creation as well as in the aspect of delivery. The relationship between the perceiver and what is perceived features in designs, product advertisements, related articles, and fashion shows in modern fashion, serving as a medium that enables the humans, who must inevitably exist between the two poles such as mind and body, the subject and the object, the ego and non-ego, and the reality and an ideal, to communicate between the poles. Humans cannot do arbitrary acts or make arbitrary selections only as they access to foreign things through instinct, desire, or experienced perception, and they are sometimes positioned passive by things. In the background, as there are human dual characteristics in which they are expressed as the ego and another ego who exists inside of the ego, they not only view an object, but also become an object to be viewed. Many products in modern fashion, as the objects of reciprocal transposition, grow giving illusions to humans. Having a desire for such objects is human's instinct and normal act to keep the life balanced between the reality and an ideal, which is based on the activation of reality function. Furthermore, freely acting rather than ignoring or overcoming the desire may be the act of retrieving one's ownership to the ego.

• Journal of the Korean Home Economics Association
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• v.43 no.12 s.214
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• pp.79-96
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• 2005

The purpose of this study is to explore the conceptions of children and their activities in everyday lives during the late period of the Goryo Dynasty. The historical method was used and the materials for analysis were the literatures written from the late thirteenth century to the fourteenth century in the Goryo Dynasty, such as 'the Ikjaijip'(익재집), 'Mokeunjip'(목은집), 'Yangchonjip'(양촌집). According to the results, the perspective on the nature of a chad was that children were inherently good with the true character which was given from natural laws and that each child was born with different natural endowment. Also, the notions on child development were that the infancy was a pure and perfect period which us not contaminated by temperament and worldly desires, instead of the developmental model that childhood is as a stage and the child must develop from the state of incompetence to the state of maturity. In addition, there were the principles for development that were keeping the innate ideal state and fostering it from the earliest time as infants were born with innate ideal state or ability. The concepts of children's characteristics were considered that they were inclined to make much noise and movement, but to have the plain and straightforward expressions and consistency in their feelings and everyday activities. And intelligent abilities for learning were often considered as desirable traits. In everyday lives, children did the household chores such as caring for livestock and silkworms or embroidering. Among their games were exploring activities, free plays using natural materials, pretend-plays, bamboo-stilts riding, and Yutnori.

• Journal of the Korean Society of Clothing and Textiles
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• v.31 no.1 s.160
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• pp.33-43
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• 2007

Middle age is the time of the most important meaning in life and also the time of physical and mental change, which offers new social activities for self-development. Middle-aged women form the major consumer class in current clothing industry, but few have been performed on this so far. The researcher studied in many aspect on the clothes which middle-aged women need to wear during this period of change. Thus this study is executed to examine what benefits middle-aged women pursue in clothing attitudes and the relationship among clothing pursuit benefit and their somatotype compensation and image orientation. The research performed the theoretical study and practical study simultaneously. The subjects are 238 middle-aged women between 35 and 49 years old in September, 2004. The results of this study are as follows: 1. The attitude of women's clothing patterns in relation with image consist of two factor structures. One is the body image and the other is the appearance image. 2. As a result of researching the attitude for choosing clothes of each body group by Rohrer index, the women with gross body group take a top priority for the lower-body compensation, while the women with slim body group take a top priority for volume compensation. 3. As a result of researching the cognitive somatotype group's attitude for choosing clothes, gross body group takes a top priority for lower-body compensation and upper-body compensation. 4. As a result of researching the relationship between real somatotype and cognitive somatotype by Rohrer index, middle-aged women think of themselves as being fatter than present state. And choosing the clothes, the body misunderstanding group of women usually show that they consider more compensation than the normally body understanding group. 5. The evaluation on real somatotype, cognitive somatotype, ideal somatotype influences on the body cathexis.