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http://dx.doi.org/10.47853/FAS.2021.e7

Effect of diluent variation on cryopreservation of large yellow croaker Larimichthys crocea  

Lim, Han Kyu (Department of Interdisciplinary Program of Biomedicine, Health & Life Convergence Sciences, Mokpo National University)
Irfan, Zidni (Department of Interdisciplinary Program of Biomedicine, Health & Life Convergence Sciences, Mokpo National University)
Lee, Hyo Bin (Department of Interdisciplinary Program of Biomedicine, Health & Life Convergence Sciences, Mokpo National University)
Song, Ji Hoon (Ocean & Fisheries Science Institute Resources Creation Research Institute)
Lee, Yun Ho (Department of Interdisciplinary Program of Biomedicine, Health & Life Convergence Sciences, Mokpo National University)
Publication Information
Fisheries and Aquatic Sciences / v.24, no.2, 2021 , pp. 63-77 More about this Journal
Abstract
The aim of this research was to investigate different factors, including cryoprotective agents (CPAs), diluents, dilution ratios, equilibrium times, freezing rates, and thawing methods to optimize cryopreservation protocols for large yellow croaker (Larimichthys crocea). The parameters evaluated were sperm motility, sperm activity index (SAI), survival rate, and DNA damage. Different types of CPAs, such as dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), methanol, and glycerol, were tested for sperm preservation. The highest motility, SAI, and survival rate were observed when EG was used. Different diluents such as Stein's solution, Hank's balanced salt solution, marine fish Ringer's solution, artificial seminal plasma (ASP) of small yellow croaker, and Cortland solution were investigated. The highest post-thaw motility was observed upon using ASP as the diluent. Different concentrations of EG were then mixed with ASP to identify the optimal EG concentration. Experimental results showed that the motility (70.33 ± 1.20%), SAI (5), and survival rate (78.30 ± 0.42%) of post-thaw sperm were optimum when 10% EG and ASP were used as the CPA and diluent of cryopreservation, respectively. Post-thaw sperm motility was high at equilibration times below 150 s and at an optimum dilution ratio of 1:1 (sperm: CPA + diluent) and was not significantly different compared with fresh sperm motility. The freezing rate was found to be slow below -10℃/min. The thawing temperature of 45℃ was identified as ideal. The percentage of tail DNA in post-thaw sperm at 10% EG and ASP was also investigated and was found to have more significant DNA damage than that in fresh sperm but significantly lower damage than that in post-thaw sperm at EG concentrations of 5%, 15%, and 20% (p < 0.05). The cryopreservation protocols obtained in this study will be useful in large yellow croaker hatcheries.
Keywords
Cryopreservation; Cryoprotective agents; Sperm motility; Large yellow croaker;
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1 Lim HK, Min BH, Jeong M, Choi BN, Le MH, Chang YJ. Cryopreservation of semen in dead yellow croaker, Larimichthys polyactis. Fish Aquat Sci. 2010;13:350-3.   DOI
2 Lim HK, Le MH. Evaluation of extenders and cryoprotectants on motility and morphology of longtooth grouper (Epinephelus bruneus) sperm. Theriogenology. 2013;79:867-71.   DOI
3 Lin DJ, Yoo YL, Chen BY. Variation of the motility and Ultrastructure of the frozen-thawed sperm in teleost, Pseudosiaena crocea (Richardson). J Fujian Norm Univ (Natur Sci Ed). 2006;22:71-6.   DOI
4 Liu QH, Ma DY, Xu SH, Xiao ZZ, Xiao YS, Song ZC, et al. Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P. olivaceus). Theriogenology. 2015;83:703-10.   DOI
5 Mounib MS. Cryogenic preservation of fish and mammalian spermatozoa. J Reprod Fertil. 1978;53:13-8.   DOI
6 Perez-Cerezales S, Martinez-Paramo S, Cabrita E, Martinez-Pastor F, de Paz P, Herraez MP. Evaluation of oxidative DNA damage promoted by storage in sperm from sex-reversed rainbow trout. Theriogenology. 2009;71:605-13.   DOI
7 Suquet M, Normant Y, Gaignon JL, Quemener L, Fauvel C. Effect of water temperature on individual reproductive activity of pollack (Pollachius pollachius). Aquaculture. 2005;243:113-20.   DOI
8 Stein H, Bayrle H. Cryopreservation of the sperm of some freshwater teleosts. Ann Biol Anim Bioch Biophys. 1978;18:1073-6.   DOI
9 Tabata K, Mizuta A. Cryopreservation of sex reversed gynogenetic female sperm in hirame. Fish Sci. 1997;63:482-3.   DOI
10 Xiao Z, Chen X, Ding F, Liu Q, Xu S, Li J. An efficient methodology of sperm ceryopreservation of large yellow croaker (Pseudosciaena croce). Mar Sci. 2007;31:1-4.
11 Lim HK, An CM, Noh GA, Min BH. Effects of diluents and cryoprotectants on sperm cryopreservation in tarry flounder (Platichthys stellatus). J Aquac. 2007;20:173-7.
12 Xu XR, Zhu JQ, Ye T, Wang CL, Zhu YF, Dahms HU, et al. Improvement of single-cell gel electrophoresis (SCGE) alkaline comet assay. Aquat Biol. 2013;18:293-5.   DOI
13 Ahn JY, Park JY, Lim HK. Effects of different diluents, cryoprotective agents, and freezing rates on sperm cryopreservation in Epinephelus akaara. Cryobiology. 2018;83:60-4.   DOI
14 Berlinsky DL, King VW, Hodson RG, Sullivan CV. Hormone induced spawning of summer flounder Paralichthys dentatus. J World Aquac Soc. 1997;28:79-86.   DOI
15 Bolla S, Holmefjord I, Refstie T. Cryogenic preservation of Atlantic halibut sperm. Aquaculture. 1987;65:371-4.   DOI
16 Xu XR, Tan FQ, Zhu JQ, Ye T, Wang CL, Zhu YF, et al. Detection of DNA damage caused by cryopreservation using a modified SCGE in large yellow croaker, Pseudosciaena crocea. Acta Biol Hung. 2014;65:405-13.   DOI
17 Zilli L, Schiavone R, Zonno V, Storelli C, Vilella S. Evaluation of DNA damage in Dicentrarchus labrax sperm following cryopreservation. Cryobiology. 2003;47:227-35.   DOI
18 Zidni I, Lee YH, Park JY, Lee HB, Hur JW, Lim HK. Effects of cryoprotective medium composition, dilution ratio, and freezing rates on spotted halibut (Verasper variegatus) sperm cryopreservation. Animals. 2020;10:2153.   DOI
19 Leung LKP. Cryopreservation of spermatozoa of the barramundi, Lates calcarifer (Teleostei: centropomidae). Aquaculture. 1987;64:243-7.   DOI
20 Suquet M, Dreanno C, Fauvel C, Cosson J, Billard R. Cryopreservation of sperm in marine fish. Aquac Res. 2000;31:231-43.   DOI
21 Bozkurt Y, Yavas I. Cryopreservation of nile tilapia (Oreochromis niloticus) sperm. Cryopreserv Eukaryotes. 2016.
22 Cabrita E, Robles V, Rebordinos L, Sarasquete C, Herraez MP. Evaluation of DNA damage in rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata) cryopreserved sperm. Cryobiology. 2005;50:144-53.   DOI
23 Fauvel C, Suquet M, Dreanno C, Zonno V, Menu B. Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions. Aquat Living Resour. 1998;11:387-94.   DOI
24 Chao NH, Liao IC. Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture. 2001;197:161-89.   DOI
25 Cheng S, Sheng Z, Zhu JQ, Wu XF. Effects of trehalose, bovine serum albumin, and sucrose, on the integrity of the plasma membrane of Pseudosciaena crocea semen after cryopreservation. Isr J Aquac Bamidgeh. 2015;67:1-12.
26 Cloud J, Patton S. Basic principles of fish spermatozoa cryopreservation. In Cabrita E, Robles V, Herraez P, editors. Method in reproductive aquaculture. Boca Raton, FL: CRC Press; 2008. p. 237.
27 Harvey B. Cryopreservation of sarotherodon mossambicus spermatozoa. Aquaculture. 1983;32:313-20.   DOI
28 Le MH, Lim HK, Min BH, Park MW, Chang YJ. Semen cryopreservation of yellow croaker Larimichthys polyactis. Rev Fish Biol Fish. 2011b;21:789-97.   DOI
29 Jiang JH, Yan JQ, Zhu JQ, Yang WX. Sperm cryopreservation and the cytoarchitecture damage detection of Pseudosciaena crocea. J Agric Biotechnol. 2011;19:725-33.   DOI
30 Kopeika E, Kopeika J, Zhang T. Cryopreservation of fish sperm. Methods Mol Biol. 2007;368:203-17.   DOI
31 Bart AN, Wolfe DF, Dunham RA. Cryopreservation of blue catfish spermatozoa and subsequent fertilization of channel catfish eggs. Trans Am Fish Soc. 1998;127:819-24.   DOI
32 Le MH, Lim HK, Min BH, Son MH, Lee JU, Chang YJ. Diluents and cryoprotectants for cryopreservation of filefish Thamnaconus modestus sperm. J Aquac. 2008;21:54-9.
33 Lee YH, Park JY, Lee IY, Zidni I, Lim HK. Effects of cryoprotective agents and treatment methods on sperm cryopreservation of stone flounder, Kareius bicoloratus. Aquaculture. 2021;531:735969.   DOI
34 Lim HK, An CM, Son MH, Park MW, Kim EO, Byun SG. Effect of diluents and temperature on sperm storage in starry flounder (Platichthys stellatus). J Aquac. 2006;19:47-51.
35 Gwo JC. Cryopreservation of black grouper (Epinephelusmalabaricus) spermatozoa. Theriogenology. 1993;39:1331-42.   DOI
36 Labbe C, Martoriati A, Devaux A, Maisse G. Effect of sperm cryopreservation on sperm DNA stability and progeny development in rainbow trout. Mol Reprod Dev. 2001;60:397-404.   DOI
37 Chang YJ. Cryopreservation of tiger puffer (Takifugu rubripes) sperm. Dev Reprod. 1997;1:29-36.
38 Withler FC, Lim LC. Preliminary observations of chilled and deep-frozen storage of grouper (Epinephelus tauvina) sperm. Aquaculture. 1982;27:389-92.   DOI
39 Betsy J, Kumar S. Cryopreservation of fish gametes. Singapore: Springer; 2020.
40 Cabrita E, Pacchiarini T, Sarasquete C, Herraez MP. 141. Development of a cryopreservation protocol for Senegalese sole testicular germ cells. Cryobiology. 2010;61:405.
41 Gwo JC. Cryopreservation of yellowfin seabream (Acanthopagrus latus) spermatozoa (Teleost, Perciformes, Sparidae). Theriogenology. 1994;41:989-1004.   DOI
42 Kho KH, Kang KH. Cryopreservation of pointhead flounder (Cleisthenes pinetorum herzensteini ) sperm. Korean J Ichthyol. 2003;15:213-8.
43 Le MH, Lim HK, Min BH, Park MS, Chang YJ. Storage of yellow croaker Larimichthys polyactis semen. Isr J Aquac Bamidgeh. 2011a;63:7.
44 Cosson J, Billard R, Cibert C, Dreanno C, Suquet M. Ionic factors regulating the motility of fish sperm. In: Gagnon C. editor. The male gamete: from basic to clinical applications. Vienna, IL: Cache Rive Press, 1999. p. 161-86.
45 Lee D, You Y. Physiological characteristics and cryopreservation of Pseudosiaena crocea (Richardson) sperms. J Trop Oceanogr. 2002;21:69-75.   DOI