• 한국농촌건축학회논문집
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• 제1권2호
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• pp.63-73
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• 1999

The study is focused on making space of the abolished primary school for college dormitory in rural area which has regional type nearby city. We, firstly, analysed regional conditions and then surveyed various data for space conditions of dormitory. By surveying exterior space and interior renovation, and analysing the actual conditions of dormitory, we propose design factors and model of using facilities of the abolished primary school. So, the purpose of it is to suggest making space and a model of dormitory utilization of abolished school in regional type nearby city. It is a spacial case of re-using facilities of abolished school in rural area, but we expect that abolished school make an offer another type of using facilities.

• 한국농촌건축학회논문집
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• 제1권1호
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• pp.107-114
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• 1999

The purpose of this study is to suggest types and a model of utilization of abolished school as facilities of the aged in rural area. There are various types of abolished school. One of them is the facilities of the aged. It is not general type, but special case according to regions. So, this study is focused on the possibility of utilization for it. We expect that abolished school make an offer a type of facilities of the aged. By surveying exterior and interior space, and analysing the actual conditions of facilities and data of various types, I propose types of abolished school, design factors and model of using facilities of the aged.

• 대한지리학회지
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• 제29권1호
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• pp.84-104
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• 1994

1960년대 이후 공업화 정책의 영향으로 농어촌 지역주민의 향도이촌현상에 의한 인 구감소와 함께 학생수도 감소되어 학교의 소규모화 등장과 나아가 폐교현상이 나타났다. 본 연구는 인구감소에 따른 폐교의 유형과 그 지역적 특성을 밝히는 것을 목적으로, 분석한 결 과 다음과 같은 점이 밝혀졌다. 1980년대 전반기에는 댐과 같은 인공시설물 건설로 인한 폐 교가 많았으나, 198O년대 후반기의 폐교는 학생수가 감소하고 10년이 지난후에 나타났으며, 경지율이 낮고 해발고도가 높은 지역에서 폐교가 더 빠르게 진행되었다. 그리고 폐교시설의 활용은 교사 건축년도와 관계없이 자연경관의 수려함과 접근성에 의한다는 점이 밝혀졌다.

• 교육시설
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• 제5권1호
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• pp.5-17
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• 1998

The migration from rural area to the city has been increased according to the rapid industrialization since 1960's. The decrement of students and teachers forced many elementary' schools in rural area to be abolished. In 1982, the Department of Education started to merge and abolish small schools by the law, and the total number of abolished schools reaches to about 1600 in present. But proper counterplan to the abolished schools has not made sufficiently. This study is to present the methodology for reusing the abolished schools through field survey and analysis of the using type in Chungnam Province.

• 교육시설 논문지
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• 제26권5호
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• pp.17-23
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• 2019

The classroom is a learning space where students live mainly in school, and it is the most important space within the entire educational space of the school. Although our educational system has been trying to revise and change the curriculum many times, it still does not provide space for the educational concept of modern society. The impact of the abolished 'school facility standard design' in 1992 is still evident in the design of school facilities at present. Specifically, the uniformity of the educational space, the rigid boundary of the classroom unit, the blockage between the school facility and the outside, and the separation due to the break of the inner and outer spaces. In the future, we need a flexible space that can contain the contents of the future education, and it is necessary to study the composition and type of educational space that can escape uniformity and spatial breakdown. In this paper, we analyze the successful cases of Irvine school facilities and examine the type and composition of classroom space, and it will be a task to find the direction and change of thinking about our educational space.

• The Korean Journal of Physiology and Pharmacology
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• 제25권2호
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• pp.159-166
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• 2021

Nicotinamide adenine dinucleotide phosphate oxidases (NOXs) are the major enzymatic source of reactive oxygen species (ROS). NOX2 and NOX4 are expressed in the heart but its role in hypoxia-induced atrial natriuretic peptide (ANP) secretion is unclear. This study investigated the effect of NOX on ANP secretion induced by hypoxia in isolated beating rat atria. The results showed that hypoxia significantly upregulated NOX4 but not NOX2 expression, which was completely abolished by endothelin-1 (ET-1) type A and B receptor antagonists BQ123 (0.3 μM) and BQ788 (0.3 μM). ET-1-upregulated NOX4 expression was also blocked by antagonists of secreted phospholipase A2 (sPLA2; varespladib, 5.0 μM) and cytosolic PLA2 (cPLA2; CAY10650, 120.0 nM), and ET-1-induced cPLA2 expression was inhibited by varespladib under normoxia. Moreover, hypoxia-increased ANP secretion was evidently attenuated by the NOX4 antagonist GLX351322 (35.0 μM) and inhibitor of ROS N-Acetyl-D-cysteine (NAC, 15.0 mM), and hypoxia-increased production of ROS was blocked by GLX351322. In addition, hypoxia markedly upregulated Src expression, which was blocked by ET receptors, NOX4, and ROS antagonists. ET-1-increased Src expression was also inhibited by NAC under normoxia. Furthermore, hypoxia-activated extracellular signal-regulated kinase 1/2 (ERK1/2) and protein kinase B (Akt) were completely abolished by Src inhibitor 1 (1.0 μM), and hypoxia-increased GATA4 was inhibited by the ERK1/2 and Akt antagonists PD98059 (10.0 μM) and LY294002 (10.0 μM), respectively. However, hypoxia-induced ANP secretion was substantially inhibited by Src inhibitor. These results indicate that NOX4/Src modulated by ET-1 regulates ANP secretion by activating ERK1/2 and Akt/GATA4 signaling in isolated beating rat hypoxic atria.

• The Korean Journal of Physiology and Pharmacology
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• 제18권2호
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• pp.135-141
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• 2014

The downregulation of A-type $K^+$ channels ($I_A$ channels) accompanying enhanced somatic excitability can mediate epileptogenic conditions in mammalian central nervous system. As $I_A$ channels are dominantly targeted by dendritic and postsynaptic processings during synaptic plasticity, it is presumable that they may act as cellular linkers between synaptic responses and somatic processings under various excitable conditions. In the present study, we electrophysiologically tested if the downregulation of somatic $I_A$ channels was sensitive to synaptic activities in young hippocampal neurons. In primarily cultured hippocampal neurons (DIV 6~9), the peak of $I_A$ recorded by a whole-cell patch was significantly reduced by high KCl or exogenous glutamate treatment to enhance synaptic activities. However, the pretreatment of MK801 to block synaptic NMDA receptors abolished the glutamate-induced reduction of the $I_A$ peak, indicating the necessity of synaptic activation for the reduction of somatic $I_A$. This was again confirmed by glycine treatment, showing a significant reduction of the somatic $I_A$ peak. Additionally, the gating property of $I_A$ channels was also sensitive to the activation of synaptic NMDA receptors, showing the hyperpolarizing shift in inactivation kinetics. These results suggest that synaptic LTP possibly potentiates somatic excitability via downregulating $I_A$ channels in expression and gating kinetics. The consequential changes of somatic excitability following the activity-dependent modulation of synaptic responses may be a series of processings for neuronal functions to determine outputs in memory mechanisms or pathogenic conditions.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1170-1175
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• 2004

The involvement of the relA and rsh genes in the morphological and physiological differentiation of Streptomyces clavuligerus was evaluated with the relA and rsh genes mutants. The morphological differentiation of S. clavuligerus was greatly affected by the disruption of the relA gene, but not very much by the disruption of the rsh gene. The altered morphological characteristics were completely restored by the complementation of the corresponding disrupted genes. Thus, it was apparent that the mycelial morphology and clavulanic acid production were severely affected by the disruption of the relA gene. Production of clavulanic acid in the submerged batch culture and glycerol-limited chemostat showed that production was inversely related to the specific growth rate in the wild-type strain. However, the production of clavulanic acid in the ${\Delta}relA$ and ${\Delta}rsh$ null mutants was completely abolished. Therefore, it seems plausible that the stringent response of S. clavuligerus to starvation for amino acids is governed mainly by ReIA, rather than Rsh, and that the (p)ppGpp synthesized immediately after the depletion of amino acids triggers the initiation of pathways for both morphological and physiological differentiation in this species.

• Molecules and Cells
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• 제25권3호
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• pp.385-389
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• 2008

Septins are a family of filament-forming GTP-binding proteins involved in a variety of cellular process such as cytokinesis, exocytosis, and membrane dynamics. Here we report the biochemical and immunocytochemical characterization of a recently identified mammalian septin, SEPT12. SEPT12 binds GTP in vitro, and a mutation (Gly56 to Asn) in the GTP-binding motif abolished binding. Immunocytochemical analysis revealed that wild-type SEPT12 formed filamentous structures when transiently expressed in Hela cells whereas $SEPT12^{G56A}$ generated large aggregates. In addition, wild-type SEPT12 failed to form filaments when coexpressed with $SEPT12^{G56A}$. We also observed that GTP-binding by SEPT12 is required for interaction with SEPT11 but not with itself.

• Molecules and Cells
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• 제24권2호
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• pp.283-287
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• 2007

$TGF-{\beta}1$ induces Ig germ-line ${\alpha}$ ($GL{\alpha}$) transcription and subsequent class switching recombination (CSR) to IgA. In the present study, we investigated the roles of two E3-ubiquitin ligases, Smurfs (HECT type) and Arkadia (RING finger type) on $TGF{\beta}1$-induced IgA CSR. We found that over-expression of Smurf1 and Smurf2 decreased $TGF{\beta}1$-induced $GL{\alpha}$ promoter activity and strengthened the inhibitory effect of Smad7 on the promoter activity. Further, over-expression of Smurf1 and Smurf2 decreased both Smad3/4-mediated and Runx3-mediated $GL{\alpha}$ promoter activities, suggesting that the Smurfs can down-regulate the major $TGF-{\beta}1$ signaling pathway and decrease $GL{\alpha}$ gene expression. In parallel, the over-expressed Smurf1 decreased the expression of endogenous IgA CSR-predictive transcripts ($GLT_{\alpha}$, $PST_{\alpha}$, and $CT_{\alpha}$) and also $TGF{\beta}1$-induced IgA secretion. Conversely over-expression of Arkadia abolished the inhibitory effect of Smad7 on $TGF{\beta}1$-induced $GLT_{\alpha}$ expression and IgA secretion. Similar results were obtained in the presence of over-expressed Smad7 and Smurf1. These results indicate that Arkadia can amplify $TGF{\beta}1$-induced IgA CSR by degrading Smad7, which interacts with Smurf1. We conclude that Smurf and Arkadia have opposite roles in the regulation of $TGF{\beta}1$-induced IgA isotype expression.