• Title/Summary/Keyword: thawing conditions

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Effects of Semen Extender Containing Equex-STM Paste on Post-thaw Motility and Viability of Canine Sperm (Equex-STM paste 첨가 희석액이 개 정액의 동결.융해 후 정자활력 및 생존율에 미치는 영향)

  • 김용준;한종현;유일정;지동범
    • Journal of Veterinary Clinics
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    • v.19 no.1
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    • pp.80-85
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    • 2002
  • This study was performed to investigate the freezomg condition especially focused on extender composition to achieve good post-thaw viability and motility of canine sperm. Semen were collected from 6 male dogs which had been proved to be fertile in the past and were treated for freezing. Equex-STM paste was contained in both the 1st(3%) and the 2nd(7%) diluent and the 2nd diluent was added to the 1st diluent following glycerol equilibration for an hour and a half. To investigate the effect of Equex-STM paste in the extender on post-thaw canine sperm characteristics, the post-thaw viability, motility, and HOS(Hypoosmotic swelling) values were evaluated according to the different composition of extender with or without Equex-STM paste, thawing conditions, and different thawing media added to thawed semen. 1. Canine sperm removed from seminal plasma and frozen )n Sweden extender containing Equex showed higher post-thaw viability, motility, and HOS values than those frozen in the extender containing Equex-STM paste with seminal plasma and those frozen in the extender without Equex and seminal plasma. 2. Canine sperm frozen in Sweden extender containing Equex-STM paste with 5% glycerol showed higher post-thaw viability, motility, and HOS values than those frozen with 3%, 8% glycerol or 5% DMSO. 3. The canine semen frozen in Sweden extender with 5% glycerol and Equex-STM paste showed higher viability, motility, and HOS values when thawed at $70^{\circ}C$ for 8 seconds than when thawed at $37.5^{\circ}C$ for 1 min and at $18-20^{\circ}C$ for 5 min. 4. TFC (tris -fructose-citrate) and PB S (phosphate buffered saline) medium added immediately to thawed canine semen brought better viability, motility, and HOS values for the sperm than those semen added with TGC(tris-glucose-citrate) and no medium. These results indicated that Equex-STM paste in Sweden extender for freezing the canine sperm which were removed from seminal plasma brought good post-thaw viability and motility of canine sperm. Also of the freezing conditions of canine sperm with the same extender containing Equex, the concentration of 5% glycerol, the thawing condition at $70^{\circ}C$ for 8 sec, and TFC and PBS medium added to the thawed semen brought better post-thaw viability and motility of canine sperm than the other conditions used in this study.

Bond Behavior of Recycled Coarse Aggregate Concrete Deteriorated by Freezing and Thawing (동결융해를 받은 순환 굵은골재 콘크리트의 부착성능)

  • Choi, Ki-Sun;Lee, Min-Jung;Yun, Hyun-Do;Kang, Ki-Woong;You, Young-Chan;Kim, Keung-Hwan
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.1097-1100
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    • 2008
  • The purpose of this study to investigate the bond strength of recycled coarse aggregate concrete deteriorated by freezing and thawing. Concrete specimens with recycled coarse aggregate representing lower limit of the quality standard (water absorption : 3.0%, specific gravity : $2.5g.cm^3$) were manufactured and tested. The replacement ratio (0, 30, 60 and 100%) of recycled coarse aggregate and freezing-thawing cycles were considered in this test. From the test results, it was found that the bond strength of normal strength concrete is not affected by the replacement ratio of recycled coarse aggregate under freezing and thawing conditions. Also, the bond strength of the natural and recycled coarse aggregate concrete using AE admixtures was not decreased by frost action.

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A study on the Freezing-Thawing Resistance for Repair Material of Concrete Structure (콘크리트 보수재료의 동결융해저항성에 관한 연구)

  • Lee, Bong-Chun;Chae, Sung-Tae;Jung, Sang-Hwa;Woo, Young-Je;Moon, Jae-Heum;Kim, Tae-Sang
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.621-624
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    • 2008
  • Repair materials which applied to the concrete structures may have different quality characteristics depending on the environmental factors. Evaluation on durability of domestic repair materials have not yet secured enough quality performance on durability, mainly due to the lack of test methods resulted from various environmental factors. In this study, we carried out the tests on freezing and thawing resistance of domestic repair materials with different environmental factors applied under BS EN 13687, and analyzed the results by comparing with Korea's national test standards(KS F 4716). The results indicate that after the repetition of dry and wet conditions and the test on freezing and thawing with salt immersion resistance bond strength might show great difference depending on the type of repair materials and the size of sample. For securing better quality performance of repair materials, it is required to establish various standards on the test methods of freezing and thawing resistance with different environmental factors applied.

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Effect of Freezing and Thawing on the Histology and Ultrastructure of Buffalo Muscle

  • Sen, A.R.;Sharma, N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.9
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    • pp.1291-1295
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    • 2004
  • Histology and transmission electron microscopy studies were carried out on buffalo muscles that were subjected to repeated freeze-thaw cycles at -10 and $-18^{\circ}C$. In the first freeze thaw cycle ($-10^{\circ}C$) structures of muscle showed slight change and closely resembled to those of normal muscle. There were frequent gaps in the half way across the fibres and some cracks in individual fibre were also noticed in second freeze thaw cycle. In the muscle frozen at $-18^{\circ}C$, more pronounced shrinkage with extensive damage of fibres with tearing was observed. The interfibrillar gaps were wider, shrinkage and tearing of the fibres were more distinct after second freeze-thaw cycle. After the second cycle, the interior portion showed large scale degradation of the ultrastructure. Our studies of buffalo muscle showed that under the proper condition, little structural damage takes place in the meat histology and ultrastructure under repeated freeze-thaw conditions. This study adds continued weight to the evidence that limited freeze-thaw cycles will not deteriorate the quality of meat.

Property Evaluation of the Freeze-Thawing for Lightweight Concrete with Development of Structural Lightweight Aggregates (구조용 경량골재 개발에 따른 경량콘크리트의 동결융해특성에 관한 연구)

  • 장동일;채원규;조광현;김광일;손영현
    • Proceedings of the Korea Concrete Institute Conference
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    • 1998.04a
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    • pp.129-136
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    • 1998
  • In this study, lightweight aggregates were developed to see the possible application as a structural uses. For the evaluation purpose, several testings were conducted to compare the physical characteristics between the controlled lightweight aggregates and other lightweight aggregates purchased from different sources. The tests included property changes of fresh concrete and strength characteristics of hardened concrete for both normal and high strength ranges. In addition, a experiment was performed to analyze the freezing and thawing resistance of new lightweight aggregate concrete against other lightweight aggregate concrete against other lightweight aggregate concretes with some experimental parameters such as lightweight aggregates, curing conditions, and water-cement ratio. The test showed that the new lightweight aggregate could be used structural components. Continuous study will be planned for future evaluations.

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Effects of Solubilization Pretreatment of Wastewater Sludge on Anaerobic Digestion (하수슬러지의 혐기성 소화에 미치는 가용화 전처리의 효과)

  • Park, Ki Young;Kim, Dae Young;Chung, Tai Hak
    • Journal of Korean Society of Water and Wastewater
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    • v.14 no.1
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    • pp.117-126
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    • 2000
  • Solubilization pretreatments were conducted to enhance the anaerobic digestion of the waste activated sludge. Four pretreatment techniques including heating, sonication freezing and thawing, and enzyme addition were employed to solubilize the waste activated sludge under various conditions. Thermal pretreatment by heating showed the highest efficiency compared with other methods, and freezing and thawing was confirmed as a feasible alternative of solubilization as well as the pretreatment of dewatering. There is a clear correlation between the solubilization efficiency of the waste activated sludge and the gas production. Batch digestion results showed the cumulative gas production as much as four times after thermal pretreatment as compared with that by the control sludge without pretreatment. As a result, hydrolysis or solubilization pretreatment might play a significant role in the high rate digestion of the waste activated sludge.

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Experimental Study on Properties of Concrete Incorporating Inoragnic Antifouling Agent (무기계 방오(防汚)제가 혼입된 콘크리트 특성에 관한 실험적 연구)

  • Kim, Yeon-Bong;Nam, Jae-Hyun
    • Journal of the Korea Institute of Building Construction
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    • v.5 no.2 s.16
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    • pp.97-105
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    • 2005
  • Concrete structures in the marine environment often deteriorate in the early stage of their service life because of contact with various aggressive conditions. In recent years, the researches on the concrete in the marine environment have been carried out to increase their service life. In this experimental study, the concrete specimens were prepared with various adding contents of inorganic antifouling agent$(0\~3.0wt\%)$ composed of some fluosilicate solution. For evaluation of the properties of concretes containing inorganic antifouling agent, various tests such as setting time, slump loss, compressive strength, water absorption rate, fleering and thawing resistance and SEM of concrete, were conducted. As the results, physical and chemical properties of concretes were improved with an adding of inorganic antifouling agent. From the results of various tests, the optimal adding contents of antifouling agent was $1.0wt\%$.

Study on the Freezing Conditions for the Frozen-Dough Preparation of Bread (냉동생지 제조를 위한 냉동조건 탐색)

  • Hahn Young-Sook
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.5
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    • pp.443-448
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    • 2004
  • In order to investigate the optimal factors for frozen dough production, the freezing and thawing condition such as temperature and time, storage period and the effect of ingredient addition were determined. A pre-fermentation of dough at 30℃ for 120 minutes was appeared to be the best for the production of frozen dough. The dough was frozen at -18℃ and then stored for 7 days. The quality of frozen dough was found to be optimal when thawed at 30℃ for 80 minutes. As ingredient of frozen dough, an addition of 3% of yeast and 4% of butter was good as well as the addition of skim milk and sugar in terms of fermentation capacity after thawing.

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Evaluation criteria for freezing and thawing of tunnel concrete lining according to theoretical and experimental analysis

  • Moon, Joon-Shik;An, Jai-Wook;Kim, Hong-Kyoon;Lee, Jong-Gun;Lattner, Tim
    • Geomechanics and Engineering
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    • v.29 no.3
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    • pp.349-357
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    • 2022
  • Abnormal climate events are occurring frequently around the world. In particular, cold waves and heavy snow lead to damage and deterioration of facilities, which can cause loss of life or property damage, such as shortening the lifespan of facilities. Therefore, it is very important to prepare an appropriate maintenance system and to establish a strategy to cope with abnormal weather conditions. In this study, laboratory freezing experiments were performed to analyze the freeze-thaw characteristics affecting the tunnel concrete lining, and heat flow analysis was carried out based on the test results. Based on these experimental and theoretical analysis results, quantitative freeze-thaw evaluation criteria for tunnel concrete linings were proposed.

Resistance to Chloride Attack of FRP Hybrid Bar After Freezing and Thawing Action (동결융해 이후의 FRP Hybrid Bar의 부식 저항성)

  • Ryu, Hwa-Sung;Park, Ki-Tae;Yoon, Yong-Sik;Kwon, Seung-Jun
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.6 no.1
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    • pp.59-65
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    • 2018
  • RC(Reinforced Concrete) structures are exposed to various exterior conditions, and the performances of both chloride resistance and freezing/thawing action are evaluated for those exposed to corrosive environment-sea shore. Recently developed FRP Hybrid Bars which is coated with glass fiber and epoxy with core steel has an engineering advantage of higher Elasticity than FRP rod. In this work, corrosion resistance, weight loss, and bond strength are evaluated for the FRP Hybrid Bar tested through freezing/thawing action for 300cycles. The double coated FRP Hybrid Bar shows the least weight loss without defection due to freezing/thawing action. Bond strength in FRP Hybrid Bar increases to 120% of normal steel through torturity effect with Si-coating. Bond strength in normal steel shows 0.86~0.89times in 3-day corrosion acceleration and 0.35~0.38times in 5-day corrosion acceleration, however, that in FRP Hybrid Bar shows little changes in bond strength before and after freezing/thawing action.