• Title/Summary/Keyword: texture control

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Influence of Soil Salinity on the Growth Response and Inorganic Nutrient Content of a Millet Cultivar (토양염농도에 따른 기장의 생장반응 및 무기양분함량 변화)

  • Kim, Sun;Ryu, Jin-Hee;Kim, Young-Joo;Jeong, Jae-Hyeok;Lee, Su-Hwan;Oh, Yang-Yeol;Kim, Young-Doo;Kim, Jae-Hyen
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.61 no.2
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    • pp.113-118
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    • 2016
  • This experiment was conducted to identify the variations in inorganic nutrients and plant growth in millet (Panicum miliaceum L.) due to soil salinity. The soil series was Munpo and soil texture was silt loam. The experimental soil was amended so that the soil had salinities of $0.8dS\;m^{-1}$, $1.6dS\;m^{-1}$, $3.2dS\;m^{-1}$ and $4.8dS\;m^{-1}$. Millet was transplanted 15 days after sowing. As soil salinity increased, the degree of reduced growth was in the order of seed production > root dry matter > plant dry matter > culm length > tiller number > stem thickness > Panicle length. Seed production was decreased to 18.9% in soil salinity of $1.6dS\;m^{-1}$, 36.9% in of $3.2dS\;m^{-1}$, and 50.7% in EC of $4.8dS\;m^{-1}$. Root dry matter decreased to 35.8% in EC of $3.2dS\;m^{-1}$, and to 40.5% in EC of $4.8dS\;m^{-1}$. As soil salinity increased, Total nitrogen content increased in all aboveground parts, roots and seeds. However, There was no difference in CaO, $P_2O_5$, $K_2O$ and, MgO in soils of different salinity. On the other hand, $Na_2O$ content was higher in the order roots> shoots> seed, and in the case of roots, $Na_2O$ content increased to 1.02% in soil salinity of $4.8dS\;m^{-1}$. However, up to soil salinity of $1.6dS\;m^{-1}$, the $Na_2O$ content of the seed was similar to that in plant grown in the Control conditions($0.8dS\;m^{-1}$). In conclusion, taking into consideration economic factors, millet could be cultivated in soil with salinities of up to approximately $1.6dS\;m^{-1}$, and seed produced from reclaimedland would be suitable for human consumption.

Characterization of quality changes of whole super sweet corn (Zea mays saccharata Sturt.) during thermal sterilization for shelf-stable products (상온유통을 위한 가열살균 중의 통 초당옥수수의 품질변화 연구)

  • Lee, Yun Ju;Yoon, Won Byong
    • Journal of Applied Biological Chemistry
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    • v.62 no.1
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    • pp.25-30
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    • 2019
  • This study investigated the quality changes in whole super sweet corn during thermal processing to extend its shelf-life. To minimize the reduction of unique texture of whole sweet corn after the sterilization, the alcohol sanitation applied and the cold point of a whole corn ear was determined using a computer simulation. The cold point was located between the corn kernel and the cob. The microorganisms on the surface of sweet corn were reduced by more than 1 log CFU/g after alcohol sanitation, then the whole corn was treated to satisfy the degree of sterilization ($F_{121.1}=4$). The quality of sterilized sweet corn was compared with the control that was treated with steaming. The quality changes of sterilized sweet corn during storage were monitored for 9 months at $25^{\circ}C$. The hardness was maintained within 30% of its initial value. The minimum of hardness was $464.50{\pm}103.35g$ and maximum of hardness was $514.50{\pm}81.83g$. The differences in the sugar content among the samples were found, but the sugar content of corn kernel remained within 30% of the control, ranging from $28.83{\pm}1.05$ to $34.36{\pm}0.42%$. The yellowness was higher than that of control by 5%. The maximum value of yellowness was $34.36{\pm}0.42$. The general bacteria and molds and yeasts in corn kernel stored at $25^{\circ}C$ were not detected after 9 months of storage at $25^{\circ}C$. Therefore, in this study, we have demonstrated that the thermal sterilized method extends the shelf-life of whole sweet corn with minimizing its quality changes over 6 months in room temperature.

Effect of TMR Feed Mixed with Whole Crop Rice on Growth Performance and Meat Quality of Hanwoo Steers (사료용 벼를 혼합한 TMR사료 급여가 한우의 생장 능력과 육질에 미치는 영향)

  • Kim, Jong Geun;Cheong, Eun Chan;Li, Yan Fen;Kim, Hak Jin;Farhad, Ahmadi;Kim, Meing Joong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.41 no.4
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    • pp.267-272
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    • 2021
  • This experiment was conducted to the purpose of evaluating the effect of feeding on Korean Native Cattle to expand the production and utilization of whole crop rice (WCR). TMR (Total mixed ration) feed was prepared by using WCR produced in Pyeongtaek, Gyeonggi-do, and the growth capacity and meat quality characteristics of 16 Korean Native Cattle raised up to 29 months of age were investigated. The produced WCR silage for feed had a moisture content of 64.02% and a crude protein content of 7.54%, and was blended with about 45% during the growing season, and lowered to 35, 15 and 9%, respectively, in the fattening period (early, middle and late stages). The body weight of the WCR-TMR feeding group was significantly higher than that of the control in the middle and late fattening stage, and at the end (29 months of age), the control group was 631 kg/head, but the WCR-TMR feeding group was 647 kg/head, which was higher. The average daily gain was significantly higher in the WCR-TMR feeding group in the growing and early fattening period, and there was no difference in the mid- and late fattening period. In the whole period, 0.71 vs 0.75 kg/head/day, WCR-TMR feeding group was high. In terms of meat quantity, the back fat thickness of the control group (11.7 mm) was significantly thicker than that of the WCR-TMR fed group (9.3 mm) (P<0.05). There was no difference in Rib eye area, Carcass weight and Meat yield index (P>0.05). In terms of meat quality, the Marbling score was higher in the WCR-TMR feeding group (P<0.05), and there were no significant differences in Meat color, Fat color, Texture and Maturity. Considering the above results, TMR feeding mainly on whole crop rice silage for feed improved the productivity of livestock, but there was no significant difference in meat quality. Therefore, it is judged that it is necessary to produce and use the whole crop rice for feed in countries with poor forage conditions.

Effect of Gypsum, Popped Rice Hull and Zeolite on Soil Aggregation in Reclaimed Tideland (간척지 토양에서 석고, 팽화왕겨 및 Zeolite 처리가 토양의 입단형성에 미치는 영향)

  • Kim, Seong-Jo;Baek, Seung-Hwa;Lee, Sang-Uk;Kim, Dae-Geun;Na, Young-Joon
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.5
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    • pp.231-237
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    • 2005
  • Gypsum treated to fine sandy loam increased the fornation of >2 mm aggregates in $1,550kg\;CaSO_4{\cdot}2H_2O\;10a^{-1}$ (Kbfg1) and $3,100kg\;CaSO_4{\cdot}2H_2O\;10a^{-1}$ (Kbfg2) to compare with control, Kc, at 60DAT, and bigger aggregates in general at 90DAT. The higher treatment of gypsum level, the <0.1 mm aggregates were less decreased as in Kbfg1, Kbfg2, and $6,200kg\;CaSO_4{\cdot}2H_2O\;10a^{-1}$ (Kbfg3) and aggregates of 0.25->2 mm were increased with increasing level of gypsum with more effective in Kbfg2 and Kbfg3 at 120DAT. Gypsum treated to silt loam increased aggregates of 2.0-1.0 and 1.0-0.5 mm in $3,100kg\;CaSO_4{\cdot}2H_2O\;10a^{-1}$ (Mbfg2) to compare with control (Mc), at 60DAT. Degrees of aggregation from 0.5-0.25 mm to >2 mm aggregates at 90DAT were distinctly higher. The higher treatment of gypsum level accelerated more aggregation of silt loam soil, and aggregates of 0.5-0.25 mm was most increased in Mbfg2 at 120DAT. Popped rice hulls treated to fine sandy loam increased aggregates of 2.0-1.0 mm in plots of $1,000kg\;10a^{-1}$ (Kbfhl) only to compare with control (Kc), at 60DAT, and aggregates of >2 mm and 2.0-1.0 mm Kbfh1 at 90DAT. At 120DAT, aggregation by popped rice hulls was most effective in Kbfbl pot. Popped rice hulls treated to silt loam increased in aggregates of >2 mm and 2.0-1.0 mm in $2000kg\;10a^{-1}$, Mbfb2 to compare with control, Mc, at 60DAT. Degrees of aggregation by popped rice hulls at 90DAT were higher in $1,000kg\;10a^{-1}$, Mbfh1, and Mbfh2, and at 120DAT was in $3,000kg\;10a^{-1}$, Mbfb3. Zeolite treatment with popped rice hulls, $1,500kg\;10a^{-1}$, increased in >2.0 mm aggregates in $1,000kg\;10a^{-1}$, Kbfbz1, $2,000kg\;10a^{-1}$, Kbfbz2, $3,000kg\;10a^{-1}$, Kbfhz3, and Mbfbz1, $1,000kg\;10a^{-1}$, Mbfbz2, $2,000kg\;10a^{-1}$, and $3,000kg\;10a^{-1}$, Mbthz3, to compare with control (Kc and Mc), at 60DAT. irrespective of soil texture. At 90DAT, >2.0-0.5 mm aggregates increased in Kbfhz1 of fine sandy loam. aggregates of >0.25 mm in $200kg\;10a^{-1}$ (Mbfbz1), $400kg\;10a^{-1}$ (Mbfhz2), $800kg\;10a^{-1}$ (Mbfhz3) of silt loam increased with the level of zeolite treatment. At 120DAT, the effect of zeolite treated to both soils showed the decrease of <0.1 mm aggregates. As the result, soil amendments for soil aggregation was more effective in the order of popped rice hulls+Zeolite > gypsum > popped rice hulls in fine sandy loam, and in the order of gypsum > popped rice huUs+zeolite > popped rice hulls in silt loam, respectively.

Studies on the Mechanical Properties of Weathered Granitic Soil -On the Elements of Shear Strength and Hardness- (화강암질풍화토(花崗岩質風化土)의 역학적(力學的) 성질(性質)에 관(關)한 연구(硏究) -전단강도(剪斷强度)의 영향요소(影響要素)와 견밀도(堅密度)에 대(對)하여-)

  • Cho, Hi Doo
    • Journal of Korean Society of Forest Science
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    • v.66 no.1
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    • pp.16-36
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    • 1984
  • It is very important in forestry to study the shear strength of weathered granitic soil, because the soil covers 66% of our country, and because the majority of land slides have been occured in the soil. In general, the causes of land slide can be classified both the external and internal factors. The external factors are known as vegetations, geography and climate, but internal factors are known as engineering properties originated from parent rocks and weathering. Soil engineering properties are controlled by the skeleton structure, texture, consistency, cohesion, permeability, water content, mineral components, porosity and density etc. of soils. And the effects of these internal factors on sliding down summarize as resistance, shear strength, against silding of soil mass. Shear strength basically depends upon effective stress, kinds of soils, density (void ratio), water content, the structure and arrangement of soil particles, among the properties. But these elements of shear strength work not all alone, but together. The purpose of this thesis is to clarify the characteristics of shear strength and the related elements, such as water content ($w_o$), void ratio($e_o$), dry density (${\gamma}_d$) and specific gravity ($G_s$), and the interrelationship among related elements in order to decide the dominant element chiefly influencing on shear strength in natural/undisturbed state of weathered granitic soil, in addition to the characteristics of soil hardness of weathered granitic soil and root distribution of Pinus rigida Mill and Pinus rigida ${\times}$ taeda planted in erosion-controlled lands. For the characteristics of shear strength of weathered granitic soil and the related elements of shear strength, three sites were selected from Kwangju district. The outlines of sampling sites in the district were: average specific gravity, 2.63 ~ 2.79; average natural water content, 24.3 ~ 28.3%; average dry density, $1.31{\sim}1.43g/cm^3$, average void ratio, 0.93 ~ 1.001 ; cohesion, $ 0.2{\sim}0.75kg/cm^2$ ; angle of internal friction, $29^{\circ}{\sim}45^{\circ}$ ; soil texture, SL. The shear strength of the soil in different sites was measured by a direct shear apparatus (type B; shear box size, $62.5{\times}20mm$; ${\sigma}$, $1.434kg/cm^2$; speed, 1/100mm/min.). For the related element analyses, water content was moderated through a series of drainage experiments with 4 levels of drainage period, specific gravity was measured by KS F 308, analysis of particle size distribution, by KS F 2302 and soil samples were dried at $110{\pm}5^{\circ}C$ for more than 12 hours in dry oven. Soil hardness represents physical properties, such as particle size distribution, porosity, bulk density and water content of soil, and test of the hardness by soil hardness tester is the simplest approach and totally indicative method to grasp the mechanical properties of soil. It is important to understand the mechanical properties of soil as well as the chemical in order to realize the fundamental phenomena in the growth and the distribution of tree roots. The writer intended to study the correlation between the soil hardness and the distribution of tree roots of Pinus rigida Mill. planted in 1966 and Pinus rigida ${\times}$ taeda in 199 to 1960 in the denuded forest lands with and after several erosion control works. The soil texture of the sites investigated was SL originated from weathered granitic soil. The former is situated at Py$\ddot{o}$ngchangri, Ky$\ddot{o}$m-my$\ddot{o}$n, Kogs$\ddot{o}$ng-gun, Ch$\ddot{o}$llanam-do (3.63 ha; slope, $17^{\circ}{\sim}41^{\circ}$ soil depth, thin or medium; humidity, dry or optimum; height, 5.66/3.73 ~ 7.63 m; D.B.H., 9.7/8.00 ~ 12.00 cm) and the Latter at changun-long Kwangju-shi (3.50 ha; slope, $12^{\circ}{\sim}23^{\circ}$; soil depth, thin; humidity, dry; height, 10.47/7.3 ~ 12.79 m; D.B.H., 16.94/14.3 ~ 19.4 cm).The sampling areas were 24quadrats ($10m{\times}10m$) in the former area and 12 in the latter expanding from summit to foot. Each sampling trees for hardness test and investigation of root distribution were selected by purposive selection and soil profiles of these trees were made at the downward distance of 50 cm from the trees, at each quadrat. Soil layers of the profile were separated by the distance of 10 cm from the surface (layer I, II, ... ...). Soil hardness was measured with Yamanaka soil hardness tester and indicated as indicated soil hardness at the different soil layers. The distribution of tree root number per unit area in different soil depth was investigated, and the relationship between the soil hardness and the number of tree roots was discussed. The results obtained from the experiments are summarized as follows. 1. Analyses of simple relationship between shear strength and elements of shear strength, water content ($w_o$), void ratio ($e_o$), dry density (${\gamma}_d$) and specific gravity ($G_s$). 1) Negative correlation coefficients were recognized between shear strength and water content. and shear strength and void ratio. 2) Positive correlation coefficients were recognized between shear strength and dry density. 3) The correlation coefficients between shear strength and specific gravity were not significant. 2. Analyses of partial and multiple correlation coefficients between shear strength and the related elements: 1) From the analyses of the partial correlation coefficients among water content ($x_1$), void ratio ($x_2$), and dry density ($x_3$), the direct effect of the water content on shear strength was the highest, and effect on shear strength was in order of void ratio and dry density. Similar trend was recognized from the results of multiple correlation coefficient analyses. 2) Multiple linear regression equations derived from two independent variables, water content ($x_1$ and dry density ($x_2$) were found to be ineffective in estimating shear strength ($\hat{Y}$). However, the simple linear regression equations with an independent variable, water content (x) were highly efficient to estimate shear strength ($\hat{Y}$) with relatively high fitness. 3. A relationship between soil hardness and the distribution of root number: 1) The soil hardness increased proportionally to the soil depth. Negative correlation coefficients were recognized between indicated soil hardness and the number of tree roots in both plantations. 2) The majority of tree roots of Pinus rigida Mill and Pinus rigida ${\times}$ taeda planted in erosion-controlled lands distributed at 20 cm deep from the surface. 3) Simple linear regression equations were derived from indicated hardness (x) and the number of tree roots (Y) to estimate root numbers in both plantations.

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Effect of High Pressure Processing on Freshness of Meat Products (육류가공품의 고압처리가 신선도에 미치는 영향 평가)

  • Hwang, Seong-Hee
    • Journal of Food Hygiene and Safety
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    • v.33 no.4
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    • pp.272-279
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    • 2018
  • The high pressure processing (HPP) is a technology which can preserve the quality of foods, such as the fresh taste, incense, texture, vitamin content, and so on, by minimizing the heating process. It does so by applying an instantaneous and uniform pressure that is the same as the water pressure that is 60 km deep in the sea. HPP is a technology that can inhibit food poisoning and spoilage caused by microorganisms and is currently an actively studied area. In this study, we investigated the effects of a high pressure treatment (0, 4, 6 min) on sliced ham, which is a typical meat product, at 600 MP a were tested for their effect on freshness. Moisture contents varied from 48 to 69%, salinity varied from 1.07 to 1.11%, and the pH decreased from 6.4~6.5 to 6.1~5.15. However, there was no difference between the control and treatment groups. General bacteria stored at $20^{\circ}C$ after hyper-pressure treatment were found to have no significant microorganisms in all groups until 4 weeks. but exceeded $10^5$ in control group and HPP 6 min treatment group from 5 weeks, At week 7, it was found to exceed $10^6$. The results indicate it was not possible to ingest food in the 4-and 6 minute treatment groups. Coliform was not observed in all groups despite observing for a total of 7 weeks at $20^{\circ}C$ weight test. VBN, a method used to determine the protein freshness of meat, showed a VBN value of less than 1 mg% until the fourth week and a value of 1 to 2 mg% after 5 weeks. The TBA was used as an index of the degree of fat acidosis in the meat tissues. The results showed it was below 0.18 mgMA / kg until the end of 7 weeks; this value was within the range for fresh meat, and there was no difference in treatment group. In this experiment, deformation of the packaging material did not occur and no swelling occurred due to the generation of gas. It is believed that the basic preservation effect was achieved only by blocking with the air due to the close contact of the packaging material.

Quality characteristics of muffins added with Pholiota adiposa powder (검은비늘버섯 분말을 첨가한 머핀의 품질특성)

  • Noh, Jae-Gwan;Yoon, Hyang-Sik;Oh, Eun Young;Kim, Ji Won;Kim, Sang Hee;Kim, Yee Gi;Han, Nam Soo;Eom, Hyun-Ju
    • Food Science and Preservation
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    • v.21 no.6
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    • pp.815-823
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    • 2014
  • This study was carried out to investigate the quality characteristics of muffins with added Pholiota adiposa powder. Muffins were prepared with different amounts of Pholiota adiposa powder (in 0, 2.5, 5, 10, and 15% ratios to the flour quantity). For analyzing the quality characteristics during the manufacturing of muffins, the pH, moisture content, dough density, height, specific volume, baking loss, texture, color, antioxidant activity, total polyphenol content, and sensory test results were determined. As the Pholiota adiposa powder content increased, the moisture content of the muffins decreased from 26.72 to 22.56%. The hardness of the muffins was increased with increasing Pholiota adiposa powder content, with the muffins with 15% Pholiota adiposa powder the hardest among all the samples. The inner color of the muffins, particularly the L and b values, decreased with increasing powder content compared to those of the control, but the a value increased. As the Pholiota adiposa powder content increased, the antioxidant activity and total polyphenol content of the muffins also increased. In the sensory evaluation, the muffins with 5% Pholiota adiposa powder showed higher values than the control and the other samples in all aspects, showing that 5% Pholiota adiposa powder could be incorporated into muffins to meet the taste and functional needs of the consumers.

Comparison of Wetting and Drying Characteristics in Differently Textured Soils under Drip Irrigation (점적관개 시 토성별 습윤.건조 특성 비교)

  • Kim, Hak-Jin;Son, Dong-Wook;Hur, Seung-Oh;Roh, Mi-Young;Jung, Ki-Yuol;Park, Jong-Min;Rhee, Joong-Yong;Lee, Dong-Hoon
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.309-315
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    • 2009
  • Maintenance of adequate soil water content during the period of crop growth is necessary to support optimum plant growth and yields. A better understanding of soil water movement for precision irrigation would allow efficient supply of water to crops, thereby resulting in minimization of water drainage and contamination of ground water. This research reports on the characterization of spatial and temporal variations in water contents through three different textured soils, such as loam, sandy loam, and loamy sand, when water is applied on the soil surface using an one-line drip irrigation system and the soils are dried after the irrigation stops, respectively. Water contents through each soil profile were continuously monitored using three Sentek probes, each consisting of three capacitance sensors at 10, 20, and 30cm depths. Spatial variability in water content for each soil type was strongly influenced by soil textural class. There were big differences in wetting pattern and the rate of downward movement between loam and sandy loam soils, showing that the loam soil had a wider wetting pattern and a slower rate of downward movement than did the sandy loam soil. The wetting pattern in loamy sand soil was not apparent due to a low variability in water content (< 10%) by a lower-water holding capacity as compared to those measured in the loam and sandy loam soils, implying that the rate of water drainage below a depth of 30cm was high. When soils were dried, there were highly exponential relationships between water content and time elapsed after irrigation stops ($r^2$${\geq}$0.98). It was estimated that equilibrium moisture contents for loam, sandy loam, and loamy sand soils would be 17.6%, 6.2%, and 4.2%, respectively.

Effects of Gamma Irradiation on the Shelf Stability of Whole Baked Egg (구운 계란의 감마선 조사에 따른 미생물학적 유통기한 설정)

  • Kim Dong-Ho;Song Hyun-Pa;Lee You-Seok;Cha Bo-Suk;Kim Byung-Keun;Byun Myung-Woo
    • Food Science and Preservation
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    • v.11 no.3
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    • pp.394-399
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    • 2004
  • The effects of gamma irradiation to improve the hygienic quality and microbiological shelf stability of whole baked egg were investigated by comparison with autoclaving process. The contamination levels of coliform, total aerobic bacteria and fungal group in a fresh egg were 10$^{5}$ CFU/g, 10$^{2}$ CFU/g and 10$^{1}$ CFU/g, respectively. After baking process, total aerobic bacteria and fungi were not exceeded to 10$^{1}$ CFU/g. Also, coliform was not detected under the aseptic process. However, cell counts of the baked egg after packaging reached to 10$^{4}$ CFU/g of total aerobic bacteria, 10$^{1}$ CFU/g of coliform, and 10$^{2}$ CFU/g of fungi. Therefore, it was assumed that microbial contamination of baked and packaged egg was mainly originated from an environmental uptake during packaging process. Microbiological shelf stability of the non sterilized control was about a week. Whereas, the baked eggs irradiated at more than 5 kGy were stable over 12 weeks at ambient condition as like those being autoclaved. Analytical texture profIle was stable within 10 kGy, but it became hardened in the sample treated with autoclaving. About 67$\%$ of panelists identified a sensory difference between non-irradiated and 10 kGy-irradiated sample. The baked egg irradiated at 10 kGy and autoclaved had lower acceptability than the control or samples irradiated lower than 5 kGy. Therefore, it was considered that optimal irradiation dose for radiation sterilization of baked and packaged egg was 5 kGy. At that point, it was recommended that appropriate microbiological shelf-life was 12 weeks at ambient condition.

FABRICATION OF MYOMUCOSAL FLAP USING CULTURED ORAL EPITHELIUM IN RABBIT MODEL (가토모델에서 배양 구강상피를 이용한 근-점막 피판의 형성에 관한 연구)

  • Shin, Young-Min;Chung, Hun-Jong;Ahn, Kang-Min;Park, Hee-Jung;Sung, Mi-Ae;Kim, Soung-Min;Hwang, Soon-Jung;Kim, Myung-Jin;Jahng, Jeong-Won;Kim, Sung-Po;Yang, Eun-Kyung;Song, Kye-Yong;Lee, Jong-Ho
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.27 no.3
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    • pp.226-237
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    • 2005
  • Purpose : Extensive defect of oral and maxillofacial area is usually reconstructed with composite flap including skin paddle. However, if the defects are lined with only skin components, the mucosa's role in mastication and texture are not restored. Furthermore, stiffness and hair-growing prevent denture rehabilitation and good oral hygiene. This study was performed to overcome the disadvantages of composite soft tissue flaps including the skin and to make a model for myo-mucosal flaps. Materials and methods : Buccal mucosa sized $0.5\times1.0\;cm^2$ from New Zealand rabbit (around 1.5kg) was harvested and cultivated by the modification of Rheinwald and Green's keratinocyte culture method. Cultured mucosa was grafted on the fascia of latismus dorsi as form of mucosal sheet. After 7, 10, 14 days, the myomucosal flap was excised and evaluated under light microscope with H & E and immunohistochemical staining. As control group, harvested buccal mucosa from rabbit was transplanted to gracilis muscle(n=6). Results : From 7 days after prelamination, the basal layer of the grafted mucosa resembled that of normal mucosa. As control group, transplanted mucosa had original shape but there's slight inflammatory reaction. Prelaminated mucosa has 19.8$\pm$4.59 cell layers and some samples have more than 20 layers. The expression rate of PCNA was relatively strong (42.9%$\pm$14.1) at the basal layer of grafted mucosa and the laminin was found at the basal layer. On the contrary, prelaminated mucosa at 10 days showed moderate expression rate of PCNA(32.4%$\pm$4.62). We found the mucosal layer was somehow disappeared and there is strong inflammatory reaction. After 14 days prelamination, the grafted oral keratinocytes were almost disappeared and expression of PCNA was not observed. Conclusion : We can make 75 fold large mucosal($3850mm^2$) sheet from small samples of mucosa $(50mm^2)$. Epithelial sheet that grafted on the fascia of muscle underwent differentiation and proliferation. But after 10, 14 days, there was strong inflammatory reaction and the grafted mucosa was destroyed from surface layer. In rabbit model, transfer of fascio-mucosal flap should be done from 7 to 10 days after prelamination.