• 한국식품과학회지
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• 제44권1호
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• pp.1-7
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• 2012

기존에 생선 검출을 위한 ELISA 개발은 여러 편 보고된 바 있으나, 고등어만을 특이적으로 검출하는 방법에 관한 보고는 거의 없었다. 본 연구에서는 고등어 parvalbumin에 대한 토끼 항체를 이용하여 가공식품 중 고등어의 검출을 위한 ELISA를 개발 하였다. 암모늄침전법과 Sephadex G-50 column chromatography를 이용하여 열에 안정한 12 kDa의 고등어 parvalbumin을 분리, 정제하였다. 여기에서 얻어진 parvalbumin을 토끼에게 면역하여 생산한 항 parvalbumin 항체 및 특이항체-HRP 복합물을 이용하여 샌드위치 ELISA(sELISA)의 조건을 확립하였다. 이때 parvalbumin 의 검출한계는 3 ng/mL이고, 고등어(생살)의 검출범위는 5-5,000 ${mu}g/mL$이었다. 이어서. 광어, 우럭, 오징어, 꽃게, 꽁치, 대구, 새우, 연어, 바다가재, 고등어에 대한 교차반응을 조사한 결과 고등어에 대한 반응성은 월등히 높았으나 다른 수산식품에서는 반응성이 거의 없거나 매우 낮게 나타나 고등어에 대한 특이성이 매우 높은 항체임을 알 수 있었다. 또한 분석시 시료의 열 안정성은 $100^{\circ}C$까지 양호하였으며 크림스프, 이유식, 소시지, 소스에 대한 spike test(0.01-0.3%)에서 분석회수율은 각각 104, 101, 54, 0%로 나타났다. sELISA에 의하여 16점의 시판시료 중 고등어의 함유 유무를 조사한 결과, 정성적으로 표시와 일치하는 것은 75%이었다. 그러므로 본 연구에서 개발한 sELISA는 일부 한계가 있기는 하지만, 가공식품 중 고등어를 정성적으로 검출하는데는 효과적으로 활용할 수 있을 것이다.

• 대한임상검사과학회지
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• 제38권3호
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• pp.184-188
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• 2006

The purpose of the recovery experiment in clinical chemistry is performed to estimate proportional systematic error. We must know all measurements have some error margin in measuring analytical performance. Proportional systematic error is the type of error whose magnitude increases as the concentration of analyte increases. This error is often caused by a substance in the sample matrix that reacts with the sought for analyte and therefore competes with the analytical reagent. Recovery experiments, therefore, are used rather selectively and do not have a high priority when another analytical method is available for comparison purposes. They may still be useful to help understand the nature of any bias revealed in the comparison of kit experiments. Recovery should be expressed as a percentage because the experimental objective is to estimate proportional systematic error, which is a percentage type of error. Good recovery is 100.0%. The difference between 100 and the observed recovery(in percent) is the proportional systematic error. We calculated the amount of analyte added by multiplying the concentration of the analyte added solution by the dilution factor(mL standard)/(mL standard + mL specimen) and took the difference between the sample with addition and the sample with dilution. When making judgments on method performance, the observed that the errors should be compared to the defined allowable error. The average recovery needs to be converted to proportional error(100%/Recovery) and then compared to an analytical quality requirement expressed in percent. The results of recovery experiments were total protein(101.4%), albumin(97.4%), total bilirubin(104%), alkaline phosphatase(89.1%), aspartate aminotransferase(102.8), alanine aminotransferase(103.2), gamma glutamyl transpeptidase(97.6%), creatine kinase(105.4%), lactate dehydrogenase(95.9%), creatinine(103.1%), blood urea nitrogen(102.9%), uric acid(106.4%), total cholesterol(108.5), triglycerides(89.6%), glucose(93%), amylase(109.8), calcium(102.8), inorganic phosphorus(106.3%). We then compared the observed error to the amount of error allowable for the test. There were no items beyond the CLIA criterion for acceptable performance.

• 보건교육건강증진학회지
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• 제26권5호
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• pp.27-39
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• 2009

목적: 이 연구의 목적은 성매매종사자들이 현행 성병검진서비스에 대해 어떻게 인식하는지를 파악하고, 이러한 인식이 성매매종사자들의 성병검진서비스 이용의도에 어떻게 관련되어 있는가를 살펴보는 데 있다. 방법: 이 연구의 자료는 보건소에서 제공하는 성병검진서비스를 이용한 경험이 있는 성매매종사자들을 대상으로 실시한 설문조사를 통해 수집되었다. 성병검진서비스에 대한 성매매종사자의 인식과 이용의도는 웨어와 그의 동료(Ware and his colleagues)들이 개발한 '환자만족도설문지(PSQ)'의 문항들로 측정하였다. 결과: 대부분의 성매매종사자들은 현재 보건소가 제공하는 성병검진서비스에 대해 만족하고 있으며, 이러한 서비스를 이용하려는 강한 의사를 지니고 있다. 성병검진서비스에 대한 일반적인 만족도와 이용의도를 예측하는 데 있어 사회경제적 변수들은 별다른 영향을 미치지 않았다. 성병검진서비스에 대한 구체적 차원들에서는 기술적인 질과 서비스에 대한 접근성이 일반적인 만족도와 이용의도에 긍정적인 방향으로 영향을 주었다. 성병검진서비스 이용의도의 가장 중요한 결정요인은 성병검진서비스에 대한 일반적인 만족도였다. 결론: 성매매특별법 시행 이후에 성매매종사자를 대상으로 하는 보건소의 성병검진사업은 크게 위축되고 있다. 이번 연구결과에서 나타난 것처럼 성매매종사자들은 보건소의 성병검진사업이 활성화되기를 희망하고 있었다. 따라서 국민의 건강 유지, 증진, 보호를 목적으로 하는 보건소는 성매매특별법의 시행과는 별도로 성병관리 사업을 계속해서 수행할 필요성이 제기된다.

• 가정과삶의질연구
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• 제2권1호
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• pp.111-129
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• 1984

The purpose of this study is to make a model of Family Life Cycle in Korea now. Answers to a questionnaire were collected from 724 housewives in Seoul area, 232 housewives in big cities, 203 housewives in small cities. The questionnaire contained 10 items about family situations,. Analyzing method employed for modeling to family life cycle are frequency, percentage, X2 -test . Results and findings are as follows; 1) The mean of first marriage age is 22.4yrs old. 23.5yrs old is the result of statistical materials published by E.P.B. 1975. The age of first marriage is higher according to the age, education & residential area. 2) The mean of first baby bearing age is 24.2 yrs old (generally 1 year after marriage). This age is the same as the result of statistical materials published by E.P.B. 3) the mean of last baby berating age is 32.6 yrs old compared to the E.P.B. statistical materials 3 yrs. low. This age is very different according to the age, education & residential area. 4) The mean of first child marriage age is 46.4 yrs old compared to the E.P.B. statistical materials 2.5 yrs old low. 5) the mean of last marriage age is 52.7 yrs old. this age is also 2.3 yrs low compared to the result of E.P.B. materials. 6) The number of child & interval is quite different according to the result of family planning generation of not. 7)According to the wife's employment, it does not show and difference. 8)The result of analyzing by F.L.C.,, we don't have launching stage & middle age stage apparently. So, we can make model of F.L.C. in Korea as follows (it will be change). 1) Establishment stage; from marriage to first baby born (23yrs old -24yrs old). 2) Child bearing & rearing stage; form first baby born to first child enter primary school(24 yrs old-30 yrs old). 3) Families with children's education stage; from first child primary school to high school graduation (30 yrs old-42yrs old) 4)Families with adult children stage; form first child got army college or stay at home(42 yrs- 48 yrs old). 5)Families with children's marriage stage; from first child marriage to last child marriage (48yrs old-57yrs old). 6) Aging stage; from last child marriage to self dying.

• 한국건설순환자원학회논문집
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• 제4권2호
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• pp.74-84
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• 2009

본 연구에서는 생산방식에 따라 생산된 고품질 순환모래의 기초물성평가를 통한 품질특성을 실험 실증적으로 평가함으로서, 최종 생산된 순환모래에 대한 성능을 평가하고, 목표 품질의 달성과 향후 성능 보완 및 활용의 확대, 고부가가치 창출을 위한 기초자료를 제시하고자 하였으며, 본 연구의 기초물성 실험결과, 직렬식 건식생산시스템과 병렬식, 소용량 습식생산시스템과 대용량을 거쳐 최종 생산된 순환모래인 RS-IV의 경우 절대건조밀도, 0.08mm체 통과량, 점토덩어리량, 유기이물질함유량에서 KS F 2573(순환잔골재) 기준에 만족하였으나, 흡수율에서는 대 소용량 습식생산시스템을 거쳐 최종 생산된 RS-IV만이 KS F 2573 기준치 5% 이하에 만족하였다. 흡수율의 품질 개선율 경우, 직렬식 건식생산시스템(7.13%), 병렬식(19.89%), 소용량 습식생산시스템(27.41%), 대용량(54.56%), 0.08mm체 통과량 경우, 직렬식 건식생산시스템(22.22%), 병렬식(39.92%), 소용량 습식생산시스템(91.89%), 대용량(98.16%), 점토덩어리량 경우, 직렬식 건식생산시스템(50.34%), 병렬식(76.77%), 소용량 습식생산시스템(98.60%), 대용량(99.32%)등으로 직렬식 건식생산시스템에 비하여 병렬식, 소용량 습식생산시스템에 비하여 대용량, 건식생산시스템에 비하여 습식생산시스템의 품질 개선율이 우수한 것으로 나타났다.

• 대한가정학회지
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• 제34권3호
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• pp.291-303
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• 1996

Generally fairytales are recognized as very effective educational materials. However, some of them includes negative prejudices on stepparents, especially stepmother. These contents are very harmful to development and adjustment of children in remarried families. Moreover, not so many studies have been done that even the results were insufficient to not only practical but also academical needs. Therefore this study purposed to investigate the parent -and stepparent- figures perceived by children with the knowledge about the related fairytales. Specific research problems are as follows; 1. How many children do know the contents of the 4 related fairytales such as Snow Whit, Sindellera, Kongjui Patjui and Changwha Honglyun? 2. What are the parent -figures perceived by the children with the knowledge about the 4 traditional fairytales? 3. What are the stepparent- figures of the subjects? 4. Is the children's sex the statistically significant variable in their parent -and stepparent- figures? 5. Is the children's age the statistically significant variable in their parent -and stepparent- figures? 6. Is the their parents' educational level the statistically significant variable in their parent -and stepparent- figures? 175 boys and girls of age -9 and- 11 were selected from 2 primary schools in Seoul. All of them were from normal families. As the steadyselling books, 18 fairytales were chosen by 7 booksellers of large scale bookstores in Seoul. And then by 10 teachers and 10 literay persons, 4 Korean and foreign traditional fairytales were seleced at last. The questionaire of 5 subareas was developed and used as the research tools, in which include 40 items. SPSS/pc program were used for data analysis for frequency, percentile, mean, and t-test. The followings are the findings with cronbach α=.80 and .94 parent -and stepparent- figures respectively. 1)The contents of the 4 tradional fairytales were read already or wellknown, even if the children who didn't. 2)The subjects showed very positive their own parent-figures such as sweet, lovely, reliable, and true or good person. 3)The subjects showed very negative stepparent-figures in contrast. 4)They showed significant sex difference(p<0.5) in their own parent-figures. And girls were more positive than boys who were a little more positive(on insignifficant level) than girls in stepmother-figures. 5)All the subjects had positive parent-figures and negative stepparent-figures. 6)Their parents education level was no significant variable in the prediction of their parent -and stepparent- figures.

• 한국응급구조학회지
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• 제12권3호
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• pp.99-113
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• 2008

In order to protect Rescue 119 workers exposed on the spot from potential infection, this study identified their awareness and practices of infection control so that it could help preventing them from infection and also provide basic materials necessary for pre-hospital infection control. This study applied questionnaire survey to total 215 Rescue 119 workers at fire stations in Jeonbuk province, Jeonnam province and Gwangju city from July 14 to Sept. 14, 2006 for the benefit of data collection. The questionnaire about possible associations between awareness and practices of infection control consisted of total 46 times across 6 categories such as washing hands during emergency activities ; fluid therapy and injection ; respirator maintenance; individual hygienics ; disinfectant supplies and equipments maintenance ; and control of infectious wastes. And collected data were processed using SPSS statistic program to analyze frequency and percentage, mean and standard deviation, Pearson's correlation coefficient, t-test and one-way ANOVA. As a result, this study came to the following conclusions : In terms of awareness about infection control, our respondents showed highest awareness about infectious waste control, and also showed highest level of practices in washing hands during emergency activities. Throughout all domains, awareness means were higher than practice means. In particular, infectious waste control was the domain of significant differences between awareness and practices. In terms of associations between awareness and individual characteristics, it was found that female rescue worker group and hospital/general hospital career group (before joining the Rescue 119) showed significantly higher awareness on statistic level. In regard to associations between individual characteristics and practices, it was found that female rescue worker group showed higher level of practices than male group on statistic level. This study also analyzed correlations between rescue workers' awareness and practice of infection control. As a result, it was found that the higher awareness was in correlations with the higher practices across all 6 domains including washing hands. In addition, the higher awareness of a questionnaire item was in significantly positive correlations with the higher practice of other items. However, our respondents showed high awareness about anti-infection, but low practices in reality. This indicates necessity of devising possible solutions to improve the practices as much as awareness. Especially, it was noted that major reasons for insufficient practices of infection control guideline come from unhabituated practices and lack of supports for infection-preventing supplies and protective device (mask, etc). Hence, it is necessary to provide more infection-preventing supplies for local rescue workers sufficiently, in parallel with steady habituation of infection control. Furthermore, it is required to manage and study infection control policies even at pre-hospital step in efforts for effective infection control, education and activities.

• 대한공업교육학회지
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• 제40권2호
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• pp.22-43
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• 2015

이 연구의 목적은 공업계 고등학교 전문교과 교사의 역할에 대한 학생들의 기대를 분석하는 것이다. 이 연구의 모집단은 공업계 고등학생이며, 472명의 공업계 고등학생을 조사대상으로 선정하였다. 자료수집은 우편조사를 통하여 2015년 6월 1일부터 6월 23일까지 이루어졌으며, 회수된 433부의 설문지 중 불성실 응답을 제외하고 428부를 최종분석에 활용하였다. 이 연구의 결과는 다음과 같았다. 첫째, 공업계 고등학생들은 전공 이론교과 수업방식으로 학생들의 수준을 고려한 수업방식을 가장 원했으며, 선호하는 실습 수업방식은 학과 계열에 따라 달랐다. 그리고 실습 수업시 안전지도 방법으로는 선생님께서 수시로 지도해 주는 방법을 가장 원했다. 둘째, 공업계 고등학생들은 교사의 생활지도시에 인성지도에 가장 관심을 가져주길 희망하였으며, 잘못한 행동에 대한 지도방법으로 차별하지 않고 대해 주길 희망하였다. 그리고 상담 주제로서 진로를 가장 원했으며, 상담시 교사가 안내자의 역할을 해주길 기대하였다. 셋째, 공업계 고등학생들은 교사의 학급경영시 화목한 학급 분위기 조성을 위해 노력해 주길 가장 희망하였으며, 교사가 학급과 학생들에게 애정을 갖고 운영해주길 희망하였다. 그리고 학생들과 친해지는 방법으로 학생들과 편하게 자주 농담을 하는 방법을 가장 희망하였다. 넷째, 공업계 고등학생들이 가장 희망하는 진로지도 내용으로는 취업과 진학 모두이었으며, 취업을 위한 상담시 학생들의 적성을 가장 고려해 주길 희망하였으며, 취업지도시 직업에 관한 정보를 가장 얻고 싶어 했다.

• 대한한의진단학회지
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• 제13권2호
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• pp.88-116
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• 2009

Background : EBM (the evidence based medicine) is the direction of the development for oriental medicine. The pulse meter-analyzer is the important part to standardize the pulse diagnosis. When we set up the direction of the study on the pulse meter-analyzer, the awereness about the pulse meter and analyzer of the clinical oriental medical doctors as consumers is very important. Objectives : In order to prepare for the mutual study and the export strategy on the pulse meter-analyzer of Korea and China, the attitude of the study and the grasp of the awereness about the pulse meter-analyzer of the Chinese medical doctors are very important. Methods : We developed the several items as the important factor of the development of the pulse meter and analyzer. They were translated by chinese medical doctor. The investigation for demend was conducted during 3 months in Guangzhou City, Guangdong Province, China. The results of the investigation was done the statistical method of frequency analysis, Chi-squared test, correspondence analysis. Results : The most important differentiation of symptom is the symptom of JANG-BU(臟腑). The method of the utility in the pulse diagnosis is Chon-Gu (寸口) pulse diagnosis and the research on Chon-Kwan-Cheok(寸關尺) is the most important measurement factor. And the typical the old pulse is the little-fine weak pulse. The pulse meter-analyzer is most suitable to the diagnosis of the hypertension and the arteriosclerosis. Conclusion : The development of the pulse analyzer including the requests of Korean and Chinese medical doctors is very important. Specially the researches on the influence factors of the traditional diagnosis and the environment of the measurement are important for developing the pulse analyze.

• 대한지구과학교육학회지
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• 제10권1호
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• pp.76-89
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• 2017

본 연구는 충북 영동군 지역의 금강과 화석산지, 다양한 지질구조를 교육자원으로 하여 과학학습장을 개발하고, 이를 활용하여 지구과학 학습과 토포 필리아를 함양할 수 있는 교육 프로그램을 구안하는 것이다. 영동 퇴적층(백악기)은 금강을 따라 신선한 노두가 잘 발달되어 있어서 다양한 지질구조와 식물화석, 공룡 발자국 화석을 쉽게 찾을 수 있고, 건열과 연흔, 사층리 등 뚜렷한 퇴적구조가 잘 나타난다. 교육과정과 교과서 분석, 전문가 패널의 협의를 통해 야외학습장(관찰지점 6곳)을 개발하여 융합교육 프로그램으로 구안했다. 개발 프로그램에 대한 내용타당도 검증 결과, 모든 검사 항목에서 양호했다($CVR{\geq}0.88$). 야외학습 모형에 따라 수업에 적용한 결과 수업 만족도는 매우 긍정적으로 평가되었다(4.18). 과학학습장에서 진행된 야외 수업과정을 관찰한 결과, 학생들은 금강의 아름다운 경관 이미지를 새롭게 형성하였으며 다양한 구체물(모래사장, 징검다리, 공룡 발자국 화석, 퇴적구조 등)에 대한 개념과 가치, 의미를 새롭게 인식하게 되면서 토포필리아를 갖게 되었다.