• Title/Summary/Keyword: ternary complex

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Adsorption Characteristics by Synthesized Goethite in the Mixed Solution Systems of Phosphate, Sulfate, and Copper Ions (합성 Goethite에 의한 인산이온, 황산이온 및 구리이온의 혼합용액에서의 흡착특성)

  • 감상규;이동환;이민규
    • Journal of Environmental Science International
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    • v.12 no.10
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    • pp.1055-1060
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    • 2003
  • Adsorption on goethite of individual component from a solution containing phosphate, sulfate, or copper ion was investigated. Competitive adsorption in the binary and ternary solution systems composed of phosphate, sulfate, and copper ions was also investigated. In competitive adsorption systems with phosphate and sulfate ions, the presence of phosphate ion reduced the adsorption of sulfate ion largely. On the other hand, the presence of sulfate ion caused only a small decrease in phosphate adsorption. This result suggests that phosphate ion is a stronger competitor for adsorption on goethite than sulfate ion, which is consistent with the higher affinity of phosphate for the surface compared to sulfate ion. Compared to the results from single-sorbate systems, adsorption of copper ion in the binary system of sulfate ion and copper ion was found to be enhanced in the presence of sulfate ion. Addition of sulfate ion to the binary system of copper ion and phosphate ion resulted in a small enhancement in copper sorption. This result implies that the presence of sulfate ion promotes adsorption of the ternary complex FeOHCuSO$_4$. The adsorption isotherms could be well described by the Langmuir adsorption equation.

Spectrophotometric Determination of Rare Earths by Ternary Complex Formation with MTB and Surfactant (글로방전 발광분광법에 의한 란탄족 원소의 정밀 분석. MTB와 계면활성제의 삼성분 착물 형성에 의한 희토류 원소의 분광광도법 정량에 관한 연구)

  • Cha Ki-Won;Yun Jeong-Sook;Kim Kyung-Hwan;Ha Young-Gu;Kim Ha-Suek
    • Journal of the Korean Chemical Society
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    • v.37 no.5
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    • pp.496-502
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    • 1993
  • Spectrophotometric determination of rare earth elements with MTB and the composition ratio were investigated in the presence of surfactants of cetylpyridinium chloride (CPC), Triton X-100, dodecyltrimethylammonium bromide (DTMAB) and cetyltrimethylammonium bromide (CTMAB) at pH 6.5. The colour development between MTB and rare earths in the presence of cationic surfactants was very stable and more sensitive than that in the absence of surfactants. The largest absorbance increase was provided by CPC, which was therefore chosen for determination of rare earth elements. REE-MTB-CPC complex has absorption maxima at 650 nm and obeys the Beer's law in the range of 0∼100 ng/ml. Molar absorptivity is $6.6{\sim}9.4{\times}10^4\;mol^{-1}l\;cm^{-1}$.

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Purification of \alpha-Cyclodextrin Glucanotransferase Excreted from Themophilic Geobacillus thermosac-chalytycus and Characterization of Transglycosylation Reaction of Glucosides. (호열성 Geobacillus thermosacchalytycus가 생산하는 \alpha-Cyclodextrin Glucanotransferase의 분리정제와 당전이 반응 특성)

  • 이미숙;신현동;김태권;이용현
    • Microbiology and Biotechnology Letters
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    • v.32 no.1
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    • pp.29-36
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    • 2004
  • $\alpha$-Cyclodextrin glucanotransferase excreted from a newly isolated Geobacillus thermosacchalytycus was purified through the ultrafiltraion, hydrophobic Sepharose CD-4B affinity chromatography, and gel filtration on Sephadex G-75, respectively. The molecular weight of the purified CGTase was 69 kDa and its N-terminal amino acid sequence was determined to be Asn-Leu-Asn-Lys-Val-Asn-Phe-Val-Ser-Asp-Val-Val-Val-Gln-Ile. The optimum pH and temperature were pH 6.0 and$ 60^{\circ}C$, respectively, and stably at the pH range of 6.0-8.0 and $60^{\circ}C$ in the presence of $Ca^{++}$. The excreted CGTase from the thermophilic G. thermosacchalytycus was $\alpha$-type showing a high coupling activity for the transglycosylation on various glucosides. The coupling reaction was carried out according to the random ternary complex mechanism.m.

Potentiometric studies on Ternary Complexes of Some Heavy Metal lons Containing N-(2-Acetamido)-iminodiacetic and Amino Acids

  • Mahmoud, Mohamed Rafat;Maher Mohamed Ahmed -Hamed;Ahmed, Iman Tawfik
    • Archives of Pharmacal Research
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    • v.16 no.1
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    • pp.78-81
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    • 1993
  • Solution equilibria of the thmary systems $UO_2(II)$, La(III), Ce(III)-N-(2-acetamido)-iminodiacetic acid and dicarboxylic amino acids are studied potentiometrically. The formation of 1:1:1 mixed ligand complexes are inferred from the potentiometric titration curves. The formation constants of the different binary and thmary complexes in such system are evaluated at t=$24\pm{0.1}^\circ{C}\;and\;\mu=0.1\;mol\;dm^{-3}(kNO_3)$. It is deduced that the temary complex is more stable than the corresponding binary amino acid anion complex. Moreover, the order og stability of the binary or temary complexes in terms of nature of amino acid and metal ion is investigated and discussed.

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The Endocrine Regulation of Chicken Growth

  • Kim, Jin-Wook
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.12
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    • pp.1668-1676
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    • 2010
  • The somatotropic axis plays a key role in proliferation and differentiation of avian organs during both pre- and posthatching periods. This review discusses the complexity of regulation of the endocrine system for chicken development and growth by growth hormone (GH), insulin-like growth factor (IGF), and IGF binding protein (IGFBP). In addition, the thyrotropic axis, including thyrotropin-releasing hormone (TRH) and thyroid hormones ($T_4$ and $T_3$), is also involved in the GH-secreting pattern. In mammals, IGFI and -II are always sequestered in a 150 kDa non-covalent ternary complex. This complex consists of one molecule each of IGF-I or IGF-II, IGFBP-3 or IGFBP-5 and an acid labile subunit (ALS). Chick ALS is identified in different strains for the first time, and further investigation of the expression of ALS on developmental stage and ALS effect on IGF bioavailability may be addressed in the future.

Determination of Acidic Drug with ISEs Using Ternary Complex of Metal-di-2-pyridyl Ketone Oxime-acidic Drug as Ion-Exchanger (이온교환체로서 금속-디-2-피리딜케톤옥심-산성의약품 3원 착물을 이용한 산성의약품의 정량)

  • 안문규;오원정;이언경;이순영;이재윤;정문모;허문회
    • YAKHAK HOEJI
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    • v.46 no.5
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    • pp.320-323
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    • 2002
  • A method for the determination of acidic drug, mefenamic acid and ibuprofen with ion-selective electrode(ISE) using Fe(II)-di-2-pyridyl ketone oxime complex as a counter ion were developed. Benzyl-2-nitrophenyl ether(BNPE) plasticized membrane was more selective and sensitive than the other tested membranes. The acidic drug selective electrode exhibits a linear response for 10$^{-2}$ M 510$^{-5}$ M of acidic drugs, mefenamic acid and ibuprofen with a slope of -55.9 and -56.3 mV/dec. in borate buffer solution (pH 8.9). Potentiometric selectivity measurements revealed negligible interferences from aromatic and aliphatic carboxylic acid salts. The electrodes were found to be useful for the direct determination of mefenamic acid and ibuprofen in pharmaceutical preparations.

Uranium(VI) Phosphate Precipitate Formation in a Carbonate Solution

  • Im, Hee-Jung;Park, Kyoung-Kyun;Park, Yeong-Jae;Kim, Won-Ho
    • Proceedings of the Korean Nuclear Society Conference
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    • 2005.05a
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    • pp.311-312
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    • 2005
  • The precipitation of U(VI) in the presence of phosphate and carbonate was investigated in the pH range of 4 to 13 and the following was obtained as a result of this experimental condition. U(VI) precipitates as a $NaUO_{2}PO_{4}$ at pH<9 but as mixtures of phosphate, hydroxides and/or carbonate at pH>9. The portion of the phosphate in the precipitate decreases almost linearly to near zero with an increasing pH in the range of 9 to 13. The U(VI) phosphate is dissolved by the carbonate complex formation at pH<10.5. The ternary complex of a carbonate and phosphate is not found.

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Spectrophotometric Determination of Scandium(III) in Monazite after Separation Using Amberlite IRC 718 Chelating Resin

  • 박찬일;차기원
    • Bulletin of the Korean Chemical Society
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    • v.20 no.12
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    • pp.1409-1412
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    • 1999
  • The spectrophotometric determination method of scandium with eriochrome cyanine R (ECR) and the composition ratio of the complex were investigated in the presence of surfactants. A volume of 5 mL of 1.0×$10^{-3}$ M ECR and 10 mL of 2.0×$10^{-4}$ M CTMAB are necessary for the determination of 1.0×$10 ^{-7}$ ~ 3.0×$10^{-6}$ M Sc(III) at pH 6.5. The apparent molar absorption coefficient of the Sc(III)-ECR-CTMAB, ternary complex at 610 nm is 5.6×$10^5$ $mol^{-1}cm{-1}$L and its detection limit is 1.0×$10^{-7}$ M. Separation studies were conducted by the column method. The effect of pH, elution solution and the influence of rare earth elements as interferents was discussed. Their separation was carried out in 0.1 M HCl-50% methanol solution and 1.0 M HCl media. The method was applied for the determination of Sc(III) in monazite.

Phosphate Sorption on Boehmite with Eu(III): P K-edge EXAFS Fingerprinting (뵈마이트 표면의 인산염 및 Eu(III) 수착: 인(P) X-선 흡수분석(EXAFS)에 의한 연구)

  • Yoon, Soh-Joung;Bleam, William F.
    • Economic and Environmental Geology
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    • v.42 no.5
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    • pp.495-500
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    • 2009
  • Actinide sorption to the geological materials can reduce the mobility and bioavailability of radionuclides released to the environment through the development of nuclear weapons and nuclear energy. Under circumneutral pH conditions, actinide sorption can be enhanced by phosphate anions sorbed on oxide mineral surfaces as indicated by the sorption of trivalent lanthanide ions ($Ln^{3+}$), the chemical analog for trivalent actinide ions ($Ac^{3+}$). In this paper, we examined a ternary sorption system of trivalent europium ions ($Eu^{3+}$) sorbed onto boehmite (${\gamma}$-AlOOH) surfaces pre-sorbed with phosphate anions (${PO_4}^{3-}$), using extended X-ray absorption fine structure (EXAFS) spectroscopy. In the Eu-$PO_4$-boehmite ternary sorption system, $EuPO_4$ surface precipitates were formed as implicated by Eu $L_{III}$-edge EXAFS spectroscopy. Phosphorus K-edge EXAFS fingerprinting indicated a bidentate mononuclear surface complex formation of phosphate sorbed on boehmite surfaces as well as $EuPO_4$ surface precipitate formation.

Procaryotic Expression of Porcine Acid-Labile Subunit of the 150-kDa Insulin-like Growth Factor Complex (미생물에서 돼지 150-kDa Insulin-Like Growth Factor Complex의 Acid-Labile Subunit 발현)

  • Lee, C. Young;Kang, Hye-Kyeong;Moon, Yang-Soo
    • Journal of Animal Science and Technology
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    • v.50 no.2
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    • pp.177-184
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    • 2008
  • Acid-labile subunit(ALS) is a 85-kDa glycosylated plasma protein which forms a 150-kDa ternary complex with 7.5-kDa insulin-like growth factor(IGF) and 40~45-kDa IGF-binding protein-3. In a previous study, the present authors prepared a porcine ALS(pALS) expression construct by inserting a pALS coding sequence into a plasmid vector following synthesis of the sequence by reverse transcription-polymerase chain reaction(RT-PCR). The expression construct, however, was subsequently found to have a mis-sense mutation at two bases of the pALS coding sequence which is presumed to have occurred through a PCR error. In the present study, the correct coding sequence was synthesized by the site-directed mutagenesis and inserted into the pET-28a(+) plasmid expression vector containing the His-tag sequence flanking the last codon of the insert DNA. After induction of the expression construct in E. coli BL21(DE3) cells, the resulting presumptive recombinant peptide was purified by the Ni-affinity chromatography. Upon SDS- PAGE, the affinity-purified peptide was resolved as a single band at a 66-kDa position which is consistent with the expected molecular mass of the presumptive recombinant pALS. Collectively, results indicate that a recombinant pALS peptide was successfully expressed and purified in the present study.