• Korean Journal of Environmental Biology
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• v.36 no.2
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• pp.131-139
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• 2018

The warming of water as a result of climate change affects fish habitat. Variations in water temperature affect fish physiology almost totally. The rise in water temperature due to climate change leads to hypoxia following decreased oxygen solubility and decreased binding capacity of oxygen-carrying hemoglobin. This study was conducted to evaluate the health status of Atlantic salmon (Salmo salar) fry at elevated water temperatures($20^{\circ}C$) compared with optimum water temperature ($15^{\circ}C$). The method facilitated the detection of biomarker genes using NGS RNAseq analysis and evaluation of their expression pattern using RT-qPCR analysis. The biomarker genes included interferon alpha-inducible protein 27-like protein 2A transcript variant X3, protein L-Myc-1b-like, placenta growth factor-like transcript variant X1, fibroblast growth factor receptor-like 1 transcript variant X1, transferrin, intelectin, thioredoxin-like, c-type lectin lectoxin-Thr1-like, ladderlectin-like and calponin-1. The selected biomarker genes were sensitive to changes in water temperature based on NGS RNAseq analysis. The expression patterns of these genes based on RT-qPCR were similar to those of NGS RNAseq analysis.

• The Korean Journal of Quaternary Research
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• v.33 no.1_2
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• pp.37-48
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• 2021

The cold surge is an important extreme weather in East Asia during winter, and is largely affected by behavior of the Siberian high Arctic Oscillation, which represents undulation of large-scale pressure pattern in the Arctic region. Recent studies also revealed that the synoptic low pressure system developing in the eastern boundary of the Asian continent is sensitive to sea surface temperature (SST) and plays an important role in strengthening the cold advection over the Korean Peninsula during cold surges. In this study, we analyzed the Arctic Oscillation affecting the large-scale background of cold surge in East Asia, and the sea surface temperature in the coast of East Asia is examined focusing on its role on synoptic low-inducing cold advection. For the analysis, the days with the bottom 3% of the average daily temperature, measured at five surface stations in Korean Peninsula during 49 years (1969/70-2017/18), were used for the cold surge cases. During the negative phase of the Arctic Oscillation, a strong trough is observed over East Asia, and the inflow of cold air from the polar region is strengthened, which lead to frequent cold surges. In addition, anomalously high SST in the eastern coast of Asia increases sensible and latent heat release from the ocean, therefore, it enlarges the likelihood of synoptic low-inducing extreme cold surges.

• Journal of Sericultural and Entomological Science
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• v.32 no.1
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• pp.31-37
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• 1990

For fine-denier cocoon filament production, the precocious trimolting silkworms were induced by the treatments with both the imidazole compound "KK-42" hating anti-juvenile hormone activity and high temperature, and their growth, dietary efficiency, cocoon and cocoon filament qualities were compared with those of normal tetramolters as control. 1. The percentage of precocious trimolters was higher in the application of KK-42 than the treatment of high temperature. The effective concentration of KK-42 was 10$\mu\textrm{g}$/1arva. The high temperature treatment for 48 hour was more effective than 36 hour treatment, and silkworm larvae fed on mulberry leaves were more sensitive than artificial diet to induce trimolters. 2. The larval duration of the trimolters induced by KK-42 was 5.17 day shorter than that of normal tetramolters in mulberry leaves rearing, and the increasing pattern of body weight in the 4th inster larvae of trimotlers was similar to the 5th instar larvae of normal tetramolters. 3. The qualities such as cocoon weight, cocoon layer weight and cocoon layer ratio of precocious trimolter induced by KK-42 were much lower those of normal tetramolters: the cocoon weight and cocoon layer ratio were 0.78g, 14.2cg and 18.4% in mulberry leaves rearing, and 0.86 cg, 10.3cg and 12.3% in artificial diet rearing, respectively. 4. The size of cocoon and cocoon filament was smaller in the precocious trimolter, both KK-42 and high temperature, as compared with that of the normal tetramolters. 5. The efficiency of cocoon layer production of the precocious trimolters by the KK-42 was lower than that of the normal tetramolters: amount of the cocoon layer production per 1g of dry mulberry leaves ingested was 7.97cg in the precocious trimotors, while 9.20cg in the normal tetramolters.

• Journal of Korea Water Resources Association
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• v.56 no.4
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• pp.285-299
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• 2023

Estimating the evapotranspiration is very important factor for effective water resources management, and FAO Penman-Monteith (FAO P-M) model has been applied for reference evapotranspiration estimation by many researchers. However, because various input data are required for the application of FAO P-M model, understanding the effect of each input data on FAO P-M model is necessary. Therefore, in this study, for 56 study stations located in South Korea, the effects of 8 meteorological factors (maximum and minimum temperature, wind speed, relative humidity, solar radiation, vapor pressure deficit, net radiation, ground heat flux), energy and aerodynamic terms of FAO P-M model, and elevation on FAO P-M reference evapotranspiration (RET) estimation were analyzed. The relative sensitivity analysis was performed to determine how 10% increment of each specific independent variable affects a reference evapotranspiration under given set of condition that other independent variables are unchanged. Furthermore, to select the 5 representative stations and perform the monthly relative sensitivity analysis for those stations, 56 study stations were classified into 5 clusters using cluster analysis. The study results showed that net radiation was turned out to be the most sensitive factor in 8 meteorological factors for 56 study stations. The next most sensitive factor was relative humidity, solar radiation, maximum temperature, vapor pressure deficit and wind speed, followed by minimum temperature in order. Ground heat flux was the least sensitive factor. In case of ground surface condition, elevation showed very low positive relative sensitivity. Relativity sensitivities of energy and aerodynamic terms of FAO P-M model were 0.707 for energy term and 0.293 for aerodynamic term respectively, indicating that energy term was more contributable than aerodynamic term for reference evapotranspiration. The monthly relative sensitivities of meteorological factors showed the seasonal effects, and also the relative sensitivity of elevation showed different pattern each other among study stations. Therefore, for the application of FAO P-M model, the seasonal and regional sensitivity differences of each input variable should be considered.

• Food Science of Animal Resources
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• v.34 no.1
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• pp.106-114
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• 2014

Folic acid, one of the B group of vitamins, is an essential substance for maintaining the functions of the nervous system, and is also known to decrease the level of homocysteine in plasma. Homocysteine influences the lowering of the cognitive function in humans, and especially in elderly people. In order to determine the strains with a strong capacity to produce folic acid, 190 bacteria were isolated from various kinds of jeotgal and chungkuk-jang. In our test experiment, JA71 was found to contain $9.03{\mu}g/mL$ of folic acid after 24 h of incubation in an MRS broth. This showed that JA71 has the highest folic acid production ability compared to the other lactic acid bacteria that were isolated. JA71 was identified as Lactobacillus plantarum by the result of API carbohydrate fermentation pattern and 16s rDNA sequence. JA71 was investigated for its physiological characteristics. The optimum growth temperature of JA71 was $37^{\circ}C$, and the cultures took 12 h to reach pH 4.4. JA71 proved more sensitive to bacitracin when compared with fifteen different antibiotics, and showed most resistance to neomycin and vancomycin. Moreover, it was comparatively tolerant of bile juice and acid, and displayed resistance to Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus with restraint rates of 60.4%, 96.7%, and 76.2%, respectively. These results demonstrate that JA71 could be an excellent strain for application to functional products.

• Food Science of Animal Resources
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• v.33 no.5
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• pp.595-602
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• 2013

As a major inhibitory neurotransmitter of the central nervous system in animals, ${\gamma}$-aminobutyric acid (GABA) has several physiological functions, such as anti-hypertensive, diuretic, tranquilizer and anti-stress effects in human. In order to determine strains with high GABA producing ability and glutamate decarboxylase (GAD) activity, 273 bacteria were isolated from various types of Kimchi. Strain K255 contained $386.37{\mu}g/mL$ of GABA in MRS broth containing 1% MSG, $600.63{\mu}g/mL$ of GABA in MRS broth containing 2% MSG and $821.24{\mu}g/mL$ of GABA in MRS broth containing 3% MSG. It showed that K255 had the highest GABA production ability compared to other commercial lactic acid bacteria. K255 was identified as Lactobacillus plantarum based on its API carbohydrate fermentation pattern and 16S rDNA sequence. K255 was investigated for its physiological characteristics. The optimum growth temperature of K255 was $37^{\circ}C$and cultures took 13 h to reach the pH 4.4. K255 showed more sensitive to bacitracin in a comparison of fifteen different antibiotics, and showed most resistance to kanamycin and vancomycin. Moreover, it was comparatively tolerant to bile juice and acid and displayed resistance to Escherichia coli, Salmonella Typhimurium, Staphylococcus aureus, with rates of 30.8%, 29.7%, and 23.4% respectively. These results demonstrate that K255 could be an excellent strain for the production of functional products.

• Journal of Navigation and Port Research
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• v.36 no.10
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• pp.803-810
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• 2012

Since the introduction of reefer container for seaborne transportation, international trade of reefer products has increased continuously with the development of refrigerate technology, increased speed of the ship and change of consumption pattern. Reefer cargo is perishable and sensitive to temperature, humidity compared with general cargo and normally reefer cargo is more valuable than general cargo. Therefore it needs special care for its handling in transit including land and sea in order to prevent cargo damage. However, lots of claims relating to reefer cargo damage rise frequently in workplace. It may increase unnecessary logistic cost and time. The aim of this study is to improve and prevent damage to reefer container cargo in transit for the purpose of benefits to both merchants and carriers to save unnecessary logistic cost and time as well as to contribute to deliver the cargo more safely and efficiently to destination.

• Progress in Superconductivity
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• v.13 no.1
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• pp.1-6
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• 2011

Measurement of magnetic signals generated from electric activity of myocardium provides useful information for the functional diagnosis of heart diseases. Key technical component of the magnetocardiography (MCG) technology is SQUID. To measure MCG signals with high signal-to-noise ratio, sensitive SQUID magnetic field sensors are needed. Present magnetic field sensors based on Nb SQUIDs have field sensitivity good enough to measure most of MCG signals. However, for accurate measurement of fine signal pattern or detection of local atrial fibrillation signals, we may need higher field sensitivity. In addition to field sensitivity, economic aspect of the SQUID system is also important. To simplify the SQUID readout electronics, the output voltage or flux-to-voltage transfer of SQUID should be large enough so that direct measurement of SQUID output can be done using room-temperature preamplifiers. Double relaxation oscillation SQUID (DROS), having about 10 times larger flux-to-voltage transfers than those of DC-SQUIDs, was shown to be a good choice to make the electronics compact. For effective cancellation of external noise inside a thin economic shielded room, first-order axial gradiometer with high balance, simple structure and long-baseline is needed. We developed a technology to make the axial gradiometer compact using direct bonding of superconductive wires between pickup coil and input coil. Conventional insert has mechanical support to hold the gradiometer array, and the dewar neck has equal diameter with the dewar bottom. Boiling of the liquid He can generate mechanical vibrations in the gradiometer array due to mechanical connection structure. Elimination of the mechanical support, and direct mounting of the gradiometer array into the dewar bottom can reduce the dewar neck diameter, resulting in the reduction of liquid He consumption.

• Korean Journal of Ecology and Environment
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• v.34 no.2 s.94
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• pp.81-89
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• 2001

In order to know relationships between green euglenoids and environmental factors, the species composition and density were assessed for 12 months together with environmental variables in the Jeonjucheon urban drainage, Korea. Nitrate was a high of 3.22 mg/1 in June and phosphate concentration was over 0.71 mg/l in the winter. The euglenoids totaled 5 genera and 71 species throughout the year, increasing in the early summer (35 to 42 texa) and decreasing in the winter (below 20 taxa). The number of green euglenoids positively correlated with surface water temperature. the total density of the green euglenoids showed a typical bimodal pattern, being maximal in the winter (5,394 cells/ml in June). The winter peak was a result of active growths of Euglena caudata, E. geniculata and E. viridis, however, each of which positively correlated with the phosphate. The early summer peak was attributed to Euglena deses, Lepocinclis ovum, and Phacus trypanon, each of which positively correlated with the ammonium and nitrate. The complete bimodal spectrum of species number and density of green euglenoids provides a sensitive image in detecting the changes of environmental variables in polluted waters such as Jeonjucheon.

• Horticultural Science & Technology
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• v.32 no.5
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• pp.607-613
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• 2014

Calcium has been associated with improved cold tolerance in many crops. The aim of this study was to investigate the changes in leaf cell $Ca^{2+}$ distribution and cell organelle ultrastructure of loquat (Eriobotrya japonica Lindl.) plants in response to cold stress at $-3^{\circ}C$, using transmission electron microscopy (TEM). Two loquat accessions, Zaozhong 6 (a commercial cultivar) and oakleaf loquat (a wild relative) were used. Cold tolerance, as measured by leaf browning rate, was higher in oakleaf plants, and calcium treatment improved cold tolerance in both species. Cold stress first induced inward transport of $Ca^{2+}$ from the intracellular space. Then, the imported $Ca^{2+}$ was aggregated around the chloroplast membrane, finally entering the chloroplast. This pattern of $Ca^{2+}$ distribution in leaf cells occurred earlier in Zaozhong 6 than in the wild loquat. With increasing time of cold exposure, the chloroplast membranes of Zaozhong 6 leaves were damaged, blurred and even disappeared, while those of wild oakleaf loquat leaves maintained their structure longer. In Zaozhong 6, cold stress induced a clear cavity between poorly structured granal thylakoids and vesicles appearing inside the chloroplast, while in oakleaf leaves cold stress had little effect on the ultrastructure of chloroplasts (although chloroplast membranes looked blurred). Loquat leaves accumulated free calcium ions around chloroplasts in response to cold stress, with earlier calcium accumulation occurring in the cold-sensitive cultivar Zaozhong 6 than in wild oakleaf loquat. These results demonstrate that these two loquat species have differences in both cold tolerance and calcium accumulation dynamics.