• 한국발생생물학회지:발생과생식
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• 제14권3호
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• pp.179-184
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• 2010

Water temperature influences on various key biological events in fish, but the internal pathway of the temperature effects are not well understood. Heat shock proteins (HSPs), known to respond in the level of cells to many environmental factors including temperature, could improve our understanding on the pathway. Some biological processes such as gonadal development and sex differentiation in the Nile tilapia Oreochromis niloticus is particularly sensitive to water temperature. In this study, we have investigated the expressions of HSP70 and HSP90 genes in young tilapia at an ordinary temperature ($28^{\circ}C$) and elevated water temperature ($36^{\circ}C$). The distribution of the expressions of HSP70 and HSP90 mRNA in this species were found to be almost ubiquitous, being detected in all tissues studied here (brain, gonad, liver and muscle), suggesting the house keeping functions of these genes. Heat shock by elevating temperature from $28^{\circ}C$ to $36^{\circ}C$ significantly increased the expression of HSP70 mRNA in the gonad, liver and muscle for several hours (P<0.05) (brain tissue was not examined for this). The increased level of HSP70 gene expression recovered to the level at control temperature ($28^{\circ}C$) when fish were kept continuously at high temperature ($36^{\circ}C$) for 24 hours. Contrary to this, expression of HSP90 mRNA did not show significant increase in the gonad and muscle by the same heat shock (P>0.05), except in the liver where the expression of HSP90 mRNA increased continuously for 24 hours at $36^{\circ}C$. The results obtained in this study suggest that response to temperature change in different tissue or organ may utilize different heat shock proteins, and that HSP70 may have some importance in temperature-sensitive gonadal event in the Nile tilapia.

• Macromolecular Research
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• 제16권7호
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• pp.659-662
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• 2008

A new and useful poly(ethylene oxide)-based pH-sensitive block copolymer is introduced. Poly(ethylene oxide-b-N-phenylmaleimide) was first synthesized by anionic polymerization of N-phenylmaleimide (N-PMI) using mixed alkali metal polymeric alkoxide by sequential monomer addition method in the mixture of benzene/THF/DMSO (10/5/3, v/v/v) at room temperature. Reductive deimidation of the resulting block copolymer was performed using hydrazine monohydrate leading to the formation of the corresponding pH-sensitive poly(ethylene oxide-b-maleic acid).

• 한국섬유공학회:학술대회논문집
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• 한국섬유공학회 2001년도 학술발표회 논문집(Proceedings of the Korean Textile Conference)
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• pp.197-200
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• 2001

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.121-121
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• 2017

The productivity of rice has been influenced by various abiotic factors including temperature which cause to limitations to rice yield and quality. Rice yield and quality are adversely affected by high temperature globally. In the present study, four Korean four cultivars such as Dongan, Ilpum, Samkwang, Chucheong were investigated in order to explore molecular mechanisms of high temperature at seedling stage. Rice seedlings grown at $28/20^{\circ}C$ (day/night) were subjected to 7-day exposure to $38/28^{\circ}C$ for high-temperature stress, followed by 2-D based proteomic analysis on biological triplicates of each treatment. The growth characteristics demonstrated that Dongan is tolerant while Ilpum is sensitive to high-temperature stress. High temperature has an adverse effect in the seedling stage both in high temperature sensitive and tolerant cultivar. Two-dimensional gels stained with silver staining, a total of 722 differential expressed protein spots (${\geq}1.5-fold$) were identified using Progenesis SameSpot software. However, a total of 38 differentially expressed protein spots were analyzed by LTQ-FT-ICR MS. Of these, 9 proteins were significantly increased while 10 decreased under high-temperature treatment. Significant changes were associated with the proteins involved in the carbohydrate metabolism, photosynthesis, and stress responses. Proteome results revealed that high-temperature stress had an inhibitory effect on carbon fixation, ATP production, and photosynthetic machinery pathway. The expression level of mRNA is significantly correlated with the results obtained in the proteome investigation. Taken together, these findings provide a better understanding of the high-temperature resistance by proteomic approaches, providing valuable insight into improving the high-temperature stress tolerance in the global warming epoch.

• The Korean Journal of Physiology
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• 제29권2호
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• pp.301-307
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• 1995

To explore electrophysiological properties of the ${\delta}-Opioid$ receptors artificially expressed in the mammalian cell, effect of an opioid agonist DPDPE $(1\;{\mu}M)$ on the voltage-sensitive outward currents was examined in the HEK293 (human embryonic kidney) cells transfected with ${\delta}-Opioid$ receptor cDNA cloned from NG-108-15 $(neuroblastoma\;{\times}\;glioma\;hybrid)$ cDNA library. Also studied were effects of 8-bromo-cyclic AMP and naloxone on DPDPE-induced changes in the voltage sensitive outward current. The voltage sensitive outward currents were recorded using perforated patch technique at room temperature. In the non-transformed HEK293 cells, DPDPE did not alter voltage sensitive outward current, indicating that no native ${\delta}-Opioid$ receptor had been developed. However, $(1\;{\mu}M)$ DPDPE remarkably increased the voltage sensitive outward current in the transformed HEK293 cells. The increment in voltage sensitive outward current peaked in $7{\sim}10\;minutes$ after DPDPE application, and the maximum DPDPE-activated outward current $(313.1{\pm}12.3\;pA)$ was recorded when the membrane potential was depolarized to +70mv. Following pretreatment of the transformed HEK293 cells with 1 mM 8-bromo-cyclic AMP, DPDPE failed to increase the voltage sensitive outward currents. On the other hand, naloxone completely abolished DPDPE-activated voltage sensitive outward current in the transformed HEK293 cells. The results of present study suggest that in the transformed HEK293 cells an activation of the ${\delta}-Opioid$ receptors by an opioid agonist DPDPE increases the voltage-sensitive potassium current as a result of decrement in cyclic AMP level.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 하계학술대회 논문집 D
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• pp.2365-2367
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• 2000

The deterministic errors of semiconductor gyros and accelerometers must be estimated and compensated to develop the low-cost IMU using them. Generally, the dominant errors of the low-cost IMU are bias and misalignment errors. Bias is sensitive to temperature. Therefore, in this paper, the effect on temperature of bias is analyzed and temperature compensation are carryed out. It is shown by experiment that the compensation is efficient.

• 한국작물학회지
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• 제64권3호
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• pp.213-224
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• 2019

There is an increasing frequency in the occurrence of abnormal weather phenomena such as sharp increases and decreases in temperature. Under these weather conditions, the heading time of rice changes unexpectedly, which poses problems in agriculture. Therefore, we investigated the effect of temperature on the heading response at different growth stages in rice. During the period from transplanting to heading, the plants were subjected to different temperature treatments, each for a 9-day period, to observe the heading response. For the heading date analysis, "heading date" was defined as the number of days from transplanting to the appearance of the first spikelet. We found that the influence of temperature increased in the order of rooting stage, followed by meiosis, early tillering, spikelet differentiation, and panicle initiation stage in all ecological types and cultivars. In particular, unlike the results reported previously, the effect of temperature on heading during the photo-sensitive period was very small. Meanwhile, the influence of temperature on vegetative growth response at different growth stages was not consistent with heading response. These results can be used as basic data for predicting the variation in heading date owing to temperature variation at each growth stage. In addition, we propose that the concept of day length should be included in determining the influence of temperature on the photo-sensitive period.

• 한국식품저장유통학회지
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• 제23권3호
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• pp.422-431
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• 2016

일부 소스류 제품에 사용된 스탠딩 파우치에서 전자레인지 가열조리 시 열변형이 발견되어 이의 원인을 규명하고자 온도 변화 패턴을 조사하였다. 전자레인지로 포장된 식품을 가열 시 포장재의 온도 변화는 식품 자체의 온도보다 높고 국소적인 온도 측정 기술을 요한다. 공시 시료로 매운맛 닭 소스와 인도카레의 전자레인지 가열 시 포장재와 시료의 온도를 열화상 카메라, 온도센서 테이프 및 광섬유 온도계를 이용하여 측정하였다. 스탠딩 파우치 형태의 포장은 전자레인지 가열 조리 시 내용물의 불균일한 가열이 관찰되어 특정 부위, 특히 액위선 상단과 측면 sealing layer에 열이 집중되는 현상이 발생하였다. 열화상 카메라를 이용한 온도 측정 방법은 식품의 표면 온도를 측정하는 제약이 있고 실제 식품의 온도보다 낮게 측정되는 경향을 보였다. 온도센서 테이프를 이용할 경우 $200^{\circ}C$까지 온도까지 측정되어 전자레인지 가열 과정 중 포장재 변형 현상이 야기될 수 있는 가능성을 확인할 수 있었다. 그리고 전자레인지 가열 시 포장재 표면의 온도 변화를 기존 광섬유 온도계로 측정할 경우 실제 온도보다 낮게 측정되는 결과가 초래되므로, 좁은 범위에서의 hot spot의 온도 변화를 감지할 수 있는 방법으로 GaAS crystal 센서를 사용함으로서 기존 센서보다 더 민감하고 정확한 온도 측정이 가능하였다.

• KSBB Journal
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• 제20권2호
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• pp.112-115
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• 2005

본 연구는 장시간의 산업적인 연속 배양에서 문제가 되는 플라즈미드 불안정성을 극복하기 위해서 박테리오 파아지람다를 벡터로 이용하였다. 또한, 벡터의 안정성과 생산성을 높이기 위해서 용균과 ${\lambda}DNA$패키징이 결핍된 돌연변이 람다를 선별하였다. 이 돌연변이 람다는 온도 전환에 의해서 단백질이 생산되는 온도 민감성 돌연변이 cI유전자를 가지고 있기 때문에 재조합 단백질 생산, ${\lambda}DNA$ 복제,숙주 세포의 안정성 등이 lytic상태로의 전환을 유도하는 온도에 영향을 받게 된다. $36^{\circ}C$의 배양 온도는 lytic으로 전환이 잘 되지 않았고, $40^{\circ}C$ 이상의 배양 온도는 완전한 lytic상태를 유도하였다. 그러나$42^{\circ}C$의 배양 온도에서는 생산성이 감소되는 온도 저해 효과가 관찰되었다. 온도가 증가할수록 박테리오 파아지가 들어 있지 않는 대장균의 수는 증가하였고,이것은 새로운 파아지를 만들 수 있는 박테리오 파이지를 사용하여 재감염을 시키면 완화될 수 있을 것으로 예상된다. 결과적으로 단위 세포당 발현량은 $40^{\circ}C$에서 최대를 나타내었고, 안정성이나 총 발현량의 관점에서는 $38^{\circ}C$가 최적의 온도로 관찰되었다.

• Smart Structures and Systems
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• 제15권2호
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• pp.395-408
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• 2015

This study aims to propose a Bayesian approach to consider changes in temperature and vehicle weight as environmental and operational factors for vibration-based long-term bridge health monitoring. The Bayesian approach consists of three steps: step 1 is to identify damage-sensitive features from coefficients of the auto-regressive model utilizing bridge accelerations; step 2 is to perform a regression analysis of the damage-sensitive features to consider environmental and operational changes by means of the Bayesian regression; and step 3 is to make a decision on the bridge health condition based on residuals, differences between the observed and predicted damage-sensitive features, utilizing 95% confidence interval and the Bayesian hypothesis testing. Feasibility of the proposed approach is examined utilizing monitoring data on an in-service bridge recorded over a one-year period. Observations through the study demonstrated that the Bayesian regression considering environmental and operational changes led to more accurate results than that without considering environmental and operational changes. The Bayesian hypothesis testing utilizing data from the healthy bridge, the damage probability of the bridge was judged as no damage.