• 지질공학
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• 제30권3호
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• pp.379-397
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• 2020

본 연구에서는 1922년부터 2019년까지 동래온천수의 장기적인 온천수 수질 변화 특성을 살펴보았다. Mann-Kendall 분석과 Sen의 기울기에 의해서 동래온천수의 장기적인 수질 특성 변화를 분석하면, 온도, Ca, SiO₂, HCO₃는 증가 추세를 보이는 반면에 EC, Na, K, Mg, Cl, SO₄은 감소 추세를 보이거나 또는 거의 추세 변화를 보이지 않는다. 동래온천수의 수질유형은 시간경과에도 변하지 않고 Na-Cl형에 안정적으로 속하고 있다. 2004년, 2009년, 2014년, 2019년의 수질 자료를 이용한 공간 분포도에 의하면, 시기별로 변동을 보이며, 시료 채취 지점에 따라 값의 변화를 보인다. 이는 장기적인 온천수 개발에도 불구하고 동래온천수의 공간적인 수질 특성은 거의 일정하다는 것을 지시한다.

• 한국미생물·생명공학회지
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• 제25권2호
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• pp.166-172
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• 1997

Aspergillus niger was selected as a strain producing the potent raw starch hydorlyzing enzyme. These experiments were conducted to investigate the conditions of the glucoa- mylase production, the purification of the enzyme, some characteristics of the purified enzyme and hydrolysis rate on various raw starches such as com, rice, potato, glutinous rice, sweet potato, wheat and barley. The optimum cultural temperature and time for the enzyme production on wheat bran medium were $30^{\circ}C$ and 96hrs, respectively. The respective addition of yeast extract and nutrient broth on wheat bran medium increased slightly the enzyme production. The enzyme was purified by ammonium sulfate fractionation and DEAE-cellulose column chromatography. The specific activity of the purified enzyme was 30.7u/mg-protein and the yield of enzyme activity was 25.8%. The purified enzyme showed a single band on polyacrylamide disc gel electrophoresis and its molecular weight was estimated to be 56,000 by SDS-polyacrylamide disc gel electrophoresis. The isoelectric point for the purified enzyme was pH3.7. The optimum temperature and pH were $65^{\circ}C$ and pH 4.0, respectively. The purified enzyme was stable in the pH range of pH 3.0-9.5 and below $45^{\circ}C$, and its thermal stability was slightly increased by the addition of $Ca^{2+}$. The purified enzyme was activated by $Co^{2+},\;Sr^{2+},\;Mn^{2+},\;Fe^{2+},\;Cu^{2+}$. Raw rice starch, raw corn starch, raw glutinous rice starch, raw sweet potato starch, raw wheat starch and raw barley starch showed more than 90% hydrolysis rate in 48hrs incubation. Even raw potato starch, most difficult to be hydrolyzed, showed 80% hydrolysis rate. The purified enzyme was identified as glucoamylase.

• Journal of Microbiology and Biotechnology
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• 제20권2호
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• pp.254-264
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• 2010

To obtain an efficient natural lignocellulolytic complex enzyme, we screened an efficient lignocellulose-degrading composite microbial system (XDC-2) from composted agricultural and animal wastes amended soil following a long-term directed acclimation. Not only could the XDC-2 degrade natural lignocelluloses, but it could also secrete extracellular xylanase efficiently in liquid culture under static conditions at room temperature. The XDC-2 degraded rice straw by 60.3% after fermentation for 15 days. Hemicelluloses were decomposed effectively, whereas the extracellular xylanase activity was dominant with an activity of 8.357 U/ml on day 6 of the fermentation period. The extracellular crude enzyme noticeably hydrolyzed natural lignocelluloses. The optimum temperature and pH for the xylanase activity were $40^{\circ}C$ and 6.0. However, the xylanase was activated in a wide pH range of 3.0-10.0, and retained more than 80% of its activity at $25-35^{\circ}C$ and pH 5.0-8.0 after three days of incubation in liquid culture under static conditions. PCR-DGGE analysis of successive subcultures indicated that the XDC-2 was structurally stable over long-term restricted and directed cultivation. Analysis of the 168 rRNA gene clone library showed that the XDC-2 was mainly composed of mesophilic bacteria related to the genera Clostridium, Bacteroides, Alcaligenes, Pseudomonas, etc. Our results offer a new approach to exploring efficient lignocellulolytic enzymes by constructing a high-performance composite microbial system with synergistic complex enzymes.

• 농업과학연구
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• 제46권4호
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• pp.769-779
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• 2019

Chilli peppers are predominantly cultivated in open field systems under abiotic and biotic stress conditions. Abiotic and biotic factors have a considerable effect on plant performance, fruit quantity, and quality. Chilli peppers grow well in a tropical climate due to their adaptation to warm and humid regions with temperatures ranging from 18 to 30℃. Nowadays, chilli peppers are cultivated all around the world under different climatic conditions, and their production is gradually expanding. Expected climate changes will likely cause huge and complex ecological consequences; high temperature, heavy rainfall, and drought have adverse effects on the vegetative and generative development of all agricultural crops including chilli peppers. To gain better insight into the effect of climate change, the growth, photosynthetic traits, morphological and physiological characteristics, yield, and fruit parameters of chilli peppers need to be studied under simulated climate change conditions. Moreover, it is important to develop alternative agrotechnologies to maintain the sustainability of pepper production. There are many conceivable ideas and concepts to sustain crop production under the extreme conditions of future climate change scenarios. Therefore, this review provides an overview of the adverse impacts of climate change and discusses how to find the best solutions to obtain a stable chilli pepper yield.

• 한국해양학회지
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• 제21권1호
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• pp.56-71
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• 1986

京畿 植物플랑크톤의 群集生態에 영향을 미치는 環境特性을 알기 위하여 物理化學的 環境要因에 대한 분석이 1981년 5월부터 1982년 9월까지 20회에 걸쳐 채취된 시료를 토대로 행하여 졌다. 物理的 조건은 灣의 入口쪽이 河口쪽보다 안정 된 상태를 보인다. 灣안쪽은 江水의 유입으로 水溫과 鹽分의 계절변화가 크게 나타 난다. 염화수로에서 팔미도까지는 담수의 영향으로 상하로 약한 두층의 흐름이 형 성된다. 팔미도 부근과 灣바깥쪽은 하계에 수온의 층상분포가 나타나나 동계에는 수온의 수직경사가 안나타난다. 灣의 안쪽과 仁川港 부근 수역은 담수의 유입과 인 천공단에서 유입된 폐수의 영향으로 오염되고 富營養化된 상태를 보인다. 대체로 낮은 수소이온 농도, 높은 생화학적 산소요구량과 높은 영양염 분포를 보인다. 그러 나 灣의 바깥쪽낮은 BOD와 높은 산소 포화도로 外海의 영향을 많이 받는 것으로 사료된다.

• 한국가스학회지
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• 제13권3호
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• pp.15-21
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• 2009

가스하이드레이트는 고압저온 조건하에서 물분자에 의해 가스분자가 포획되어 이루어진 얼음형태의 고체상 결정물로 정의되며, 가스 파이프라인 또는 천연가스 생산시설에서의 하이드레이트 형성은 심각한 유동저해 문제를 야기할 수 있다. 반면에 다양한 분야에 응용 잠재력을 갖고 있는 가스하이드레이트의 저장밀도 특성 및 안정성은 천연가스 저장 및 수송에 획기적인 수단으로 활용될 수 있다. 그러나 형성과정의 복잡성 및 느린 반응, 비용 등의 문제는 여전히 해결해야할 주요과제로 남아 있다. 본 연구에서는 가스하이드레이트의 인공생성에 있어, 압력과 온도, 물함량 대비 가스저장비율, SDS의 농도, 교반 및 비교반 효과와 같은 각 주요인자별로 하이드레이트 생성성능에 미치는 영향을 실험적 방법을 통하여 비교함으로써 그 상관관계를 규명하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.373-373
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• 2014

Resistive-change random access memory (ReRAM) device is one of the promising candidates owing to its simple structure, high scalability potential and low power operation. Many resistive switching devices using transition metal oxides materials such as NiO, Al2O3, ZnO, HfO2, $TiO_2$, have attracting increased attention in recent years as the next-generation nonvolatile memory. Among various transition metal oxides materials, HfO2 has been adopted as the gate dielectric in advanced Si devices. For this reason, it is advantageous to develop an HfO2-based ReRAM devices to leverage its compatibility with Si. However, the annealing temperature of these high-k thin films for a suitable resistive memory switching is high, so there are several reports for low temperature process including microwave irradiation. In this paper, we demonstrate the bipolar resistive switching characteristics in the microwave irradiation annealing processed Ag/HfO2/Pt ReRAM device. Compared to the as-deposited Ag/HfO2/Pt device, highly improved uniformity of resistance values and operating voltage were obtained from the micro wave annealing processed HfO2 ReRAM device. In addition, a stable DC endurance (>100 cycles) and a high data retention (>104 sec) were achieved.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.352.1-352.1
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• 2014

Indium Tin Oxide (ITO) films are the most extensively studied and commonly used as ones of TCO films. The ITO films having a high electric conductivity and high transparency are easily fabricated on glass substrate at a substrate temperature over $250^{\circ}C$. However, glass substrates are somewhat heavy and brittle, whereas plastic substrates are lightweight, unbreakable, and so on. For these reasons, it has been recently suggested to use plastic substrates for flexible display application instead of glass. Many reaearchers have tried to produce high quality thin films at rood temperatures by using several methods. Therefore, amorphous ITO films excluding thermal process exhibit a decrease in electrical conductivity and optical transparency with time and a very poor chemical stability. However the amorphous Indium Gallium Zinc Oxide (IGZO) offers several advantages. For typical instance, unlike either crystalline or amorphous ITO, same and higher than a-IGZO resistivity is found when no reactive oxygen is added to the sputter chamber, this greatly simplifies the deposition. We reported on the characteristics of a-IGZO thin films were fabricated by RF-magnetron sputtering method on the PEN substrate at room temperature using 3inch sputtering targets different rate of Zn. The homogeneous and stable targets were prepared by calcine and sintering process. Furthermore, two types of IGZO TFT design, a- IGZO source/drain material in TFT and the other a- ITO source/drain material, have been fabricated for comparison with each other. The experimental results reveal that the a- IGZO source/drain electrode in IGZO TFT is shown to be superior TFT performances, compared with a- ITO source/drain electrode in IGZO TFT.

• 한국재료학회지
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• 제20권5호
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• pp.252-256
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• 2010

The study was done to change the morphology and pore size of SBA-15 silica, and the characteristics of SBA-15 silica were investigated with TG-DSC, XRD, SEM, TEM and N2 adsorption-desorption under changing aging conditions. SBA-15 silica having a 2D-hexagonal structure was synthesized and confirmed by SEM and TEM. The structure of mesoporus silica SBA-15 showed a pore having regularly formed hexagonal structure and a passage having a cylindrical shape. This result is in good agreement with the pore forming in XRD and cylindrical shape of the structure in $N_2$ adsorption-desorption isotherm. SBA-15 silica showed a large BET surface area of $603-698\;m^2/g$, a pore volume of $0.673-0.926\;cm^3/g$, a large pore diameter of 5.62-7.42 nm, and a thick pore wall of 3.31-4.37 nm. This result shows that as the aging temperature increases, the BET surface area, pore volume, and pore diameter increase but the pore wall thickness decreases. The BET surface areas in SM-2 and SM-3 are as large as $698\;m^2/g$. However, SM-2 has a large surface area and forms a thick pore wall, when the aging temperature is $100^{\circ}C$ and is synthesized into stable mesoporous SBA-15 silica.

• 미생물학회지
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• 제35권3호
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• pp.231-236
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• 1999

Penicillium oxalicum 으로부터 세포외로 분비되는 Anabaena cylindrica 분해효소의 분자량은 renaturation SDS-PAGE에서 약 22kDa 으로 확인되었으며, 분해 효소의 농축은 ultrafiltration cut off fraction 중 30-10 kDa 구간에서 수획하였다. 최적 활성조건의 측정 결과 적정 pH는 3.5-4.0, 적정반응 온도는 $20^{\circ}C$, 그리고 온도 안정성은 $4^{\circ}C$ 이하에서 100% 이상, 20-$90^{\circ}C$ 범위에서는 50% 이상의 활성을 나타내었다. 금속이온 및 효소안정제의 영향에서는 $Na^+$,$K^+$, $Ba^(2+)$, $Mg^(2+)$, $Mn^(2+)$의 양이온과 BSA는 효소의 활성을 촉진시키는 반면, $Ca^(2+)$, $Cu^(2+)$의 양이온과 EDTA, PMSF 는 효소의 활성을 억제하는 작용을 하였다. 이러한 금속이온과 안정제의 영향에서 1가, 2가 양이온에 의해 활성이 증가하고, $Fe^(3+)$, $Ca^(2+)$, $Cu^(2+)$의 양이온에 의해서는 활성이 감소하는 결과는 대부분의 세포벽 분해효소가 갖는 특성과 유사한 결과였다. 분해효소는 A. cylindrica 과 Micrococcus. luteus 의 세포벽을 기질로 사용한 효소의 활성 반응에서 반응 시작 후 1시간에서 5시간 사이에 반응 산물로 환원당의 양이 급격히 증가하였다.