• BMB Reports
• /
• v.30 no.3
• /
• pp.167-172
• /
• 1997

The yeast Saccharomyces cerevisiae, mutant CH117, shows a drug-hypersensitivity (dhs) to cycloheximide, bleomycin, actinomycin D, 5-fluorouracil. nystatin, nigericin and several other antibiotics. CH 117 was also temperature-sensitive (ts). being unable to grow at $37^{\circ}C$ and secreted more invertase and acid phosphatase into the medium than the parent yeast. CH117 grows very slowly and the cell shape is somewhat larger and more sensitive to zymolyase than the wild type cells. Light microscopic and electron microscopic observation also revealed abnormality of the mutant cell wall. These characteristics indicate that CH117 has a defect in an essential component of the cell surface and that the cell wall which performs barrier functions has become leaky in the mutant. We screened a genomic library of wild type yeast for clones that can complement the mutation of CH117. A plasmid, pCHX1, with an insert of 3.6 kilobases (kbs) could complement the dhs and ts of CH117. Deletion and subcloning of the 3.6 kb insert showed that a gene for the complementation of mutant phenotypes was located in 1.9 kbs Puvll-Hindlll fragment.

• Fisheries and Aquatic Sciences
• /
• v.7 no.3
• /
• pp.122-129
• /
• 2004

Harmful Cochlodinium polykrikoides is a notorious harmful algal bloom (HAB) species that is causing mass mortality of farmed fish along the Korean coast with increasing frequency. We analyzed the sequence of the large subunit (LSD) rDNA D1-D3 region of C. polykrikoides and conducted phylogenetic analyses using Bayesian inference of phylogeny and the maximum likelihood method. The molecular phylogeny showed that C. polykrikoides had the genetic relationship to Amphidinium and Gymnodinium species supported only by the relatively high posterior probabilities of Bayesian inference. Based on the LSU rDNA sequence data of diverse dinoflagellate taxa, we designed the C. polykrikoides-specific PCR primer set, CPOLY01 and CPOLY02 and developed PCR detection assays for its sensitive, accurate HAB monitoring. CPOLY01 and CPOLY02 specifically amplified C. polykrikoides and did not cross-react with any dinoflagellates tested in this study or environmental water samples. The effective annealing temperature $(T_{p})$ of CPOLY01 and CPOLY02 was $67^{\circ}C$. At this temperature, the conventional and nested PCR assays were sensitive over a wide range of C. polykrikoides cell numbers with detection limits of 0.05 and 0.0001 cells/reaction, respectively.

• The Transactions of the Korean Institute of Electrical Engineers
• /
• v.34 no.10
• /
• pp.389-398
• /
• 1985

Recently maximum power demand of our country has become to be under the great in fluence of electric cooling and air conditioning demand which are sensitive to weather conditions. This paper presents the technique and algorithm to forecast the long-term maximum power demand considering the characteristics of electric power and weather variable. By introducing a weather load model for forecasting long-term maximum power demand with the recent statistic data of power demand, annual maximum power demand is separated into two parts such as the base load component, affected little by weather, and the weather sensitive load component by means of multi-regression analysis method. And we derive the growth trend regression equations of above two components and their individual coefficients, the maximum power demand of each forecasting year can be forecasted with the sum of above two components. In this case we use the coincident dry bulb temperature as the weather variable at the occurence of one-day maximum power demand. As the growth trend regression equation we choose an exponential trend curve for the base load component, and real quadratic curve for the weather sensitive load component. The validity of the forecasting technique and algorithm proposed in this paper is proved by the case study for the present Korean power system.

• Journal of the Korean Applied Science and Technology
• /
• v.17 no.4
• /
• pp.262-266
• /
• 2000

Acrylic pressure sensitive adhesives of n-butyl acrylate, 2-ethyl acrylate, methyl acrylate, vinyl acetate, acrylic acid, acrylonitrile and 2-hydroxyethyl acrylate were synthesized and basic physical properties of pressure sensitive adhesives with increasing the contents of 2-hydroxyethyl acrylate were investigated. 2-Hydroxyethyl acrylates effects on glass transition temperature, viscosity, hardening time and peel strength. Glass transition temperature(Tg) decreased with increasing the contents of 2-hydroxyethyl acrylate. Viscosity and hardening time were increased with increasing the contents of 2-hydroxyethyl acrylate. On the other hands, peel strength increased with increasing the contents of 2-hydroxyethyl acrylate up to 6 wt% and the decreased at further higher contents of 2-hydroxyethyl acrylate. In peel test, interfacial failure was occured in 8 wt% and 10wt%.

• Macromolecular Research
• /
• v.8 no.1
• /
• pp.26-33
• /
• 2000

A series of pH/temperature sensitive polymers were synthesized by copolymerizing N-isopro-pyl acrylamide(NIPAAm) and acrylic acid(AAc) . The influence of polyelectrolyte between poly(allyl amine) (PAA) and poly(L-lysine)(PLL) on the lower critical solution temperature(LCST) of pH/temperature sensitive polymer was compared in the range of pH 2∼12. The LCST of PNIPAAm/water in aqueous poly(NIPAAm-co-AAc) solution was determined by cloud point measurements. A polyelectrolyte complex was prepared by mixing poly(NIPAAm-co-AAc) with poly(allyl amine) (PAA) or poly(L-lysine) (PLL) solutions as anionic and cationic polyelectrolytes, respectively. The effect of polyelectrolyte complex formation on the conformation of PLL was studied as a function of temperature by means of circular dichroism(CD). The cloud points of PNIPAAm in the aqueous copolymers solutions were stongly affected by pH, the presence of polyelectrolyte solute, AAc content, and charge density. The polyelectrolyte complex was formed at neutral condition. The influence of more hydrophobic PLL as a polyelectrolyte on the cloud point of PNIPAAm in the aqueous copolymer solution was stronger than that of poly(allyl amine)(PAA). Although polymer-polymer complex was formed between poly(NIPAAm-co-AAc) and PLL, the conformational change of PLL did not occur due to steric hinderance of bulky N-isopropyl groups of PNIPAAm.

• Journal of the Korean Institute of Gas
• /
• v.13 no.4
• /
• pp.33-39
• /
• 2009

This study is intended to experiment performance of pressure relief device and to extend the effective ways to prevent cylinders of NGV from bursting when they are exposed to local fire intensively or when they are overcharged under ambient temperature at fueling stations in summer. In the results of thermal cycling experiments, all products of three companies met the requirements for gas leakage in the qualification criteria between $82^{\circ}C$ and $-40^{\circ}C$. But the o-rings of two companies' specimens among the three companies' specimens got damaged under the accelerated conditions between $135^{\circ}C$ and $-45^{\circ}C$. It took one minute and thirty nine seconds for a glass bulb type of a thermal sensitive type PRD to activate and it took two minutes and thirty one seconds for a fusible plug type of a thermal sensitive type PRD to activate. These results indicated that a glass bulb type of a thermal sensitive type PRD was one minute faster than a fusible plug type of a thermal sensitive type PRD. Under the accelerated condition $135^{\circ}C$, the activation pressure of a pressure sensitive type PRD burst at 32.1 MPa and, under the condition of qualification criteria, it burst from 30.7 MPa to 32.1 MPa.. As a result of the experiment for performance of pressure relief device, in the case of the thermal sensitive type PRD, a glass bulb type is more effective to flame than a fusible plug type. we confirmed that the rupture pressure of a pressure sensitive type PRD could not be affected by temperature and pressure cycling.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 1995.05a
• /
• pp.90-95
• /
• 1995

This paper describes the highly-sensitive Si magnetic sensor using the amplitude modulation in order to real ice the integrated magnetic sensor which to sensor a weak magnetic field. Generally, the most important two parameters in Hall IC which degrade the ability of magnetic detection are the variation of offset according to the variation of temperature and the noise of amplifiers. In this paper, we use a Hall element and compensator to reduce the offset and the nouse of amplifiers by Using amplititude modulation method.

• 전기의세계
• /
• v.31 no.5
• /
• pp.368-374
• /
• 1982

This paper describes a combined algorithm for short-term-load forecating. One of the specific features of this algorithm is that the base, weather sensitive and residual components are predicted respectively. The base load is represented by the exponential smoothing approach and residual load is represented by the Box-Jenkins methodology. The weather sensitive load models are developed according to the information of temperature and discomfort index. This method was applied to Korea Electric Company and results for test periods up to three years are given.

• Coupled systems mechanics
• /
• v.5 no.4
• /
• pp.315-327
• /
• 2016

Undergoing large volumetric changes upon incremental environmental stimulation, hydrogels are interesting materials which hold immense potentials for utilization in a wide array of applications in diverse industries. Owing to the large magnitudes of deformation it undergoes, swelling induced instability is a commonly observed sight in all types of gels. In this work, we investigate the instability of photo-thermal sensitive hydrogels, produced by impregnating light absorbing nano-particles into the polymer network of a temperature sensitive hydrogel, such as PNIPAM. Earlier works have shown that by using lights of different intensities, these hydrogels follow different swelling trends. We investigate the possibility of utilizing this fact for remote switching applications. The analysis is built on a thermodynamic framework of inhomogeneous large deformation of hydrogels and implemented via commercial finite element software, ABAQUS. Various examples of swelling induced instabilities, and its corresponding dependence on light intensity, will be investigated. We show that the instabilities that arise have their morphologies dependent on the light intensity.

• Journal of Microbiology
• /
• v.35 no.4
• /
• pp.334-340
• /
• 1997

A collection of 132 temperature sensitive (ts) mutants was generated by the chemical mutagenesis of Schizosaccharomyces pombe wild type strain and screened for tRNA splicing defects on Northern blots by hybridization with an oligonucleotide that recognizes the exon of the S. pombe tRNA^Val as a probe. We identidied 6 mutants which accumulate precursor $tRNA^{Val}$. Among them, 2 mutants exhibited remarkable morphological differences compared to wild type cells. One tRNA splicing mutant showed elongated cell shape in permissive as well as non-permissive cultures. The other mutant exhibited shortened cell morphology only in nonpermissive culture. The total RNA pattern in the splicing mutants appeared to be normal. Genetic analysis of four $tRNA^{Val}$ splicing mutants demonstrated that the mutation reside in different genes.