• 센서학회지
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• 제2권1호
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• pp.35-40
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• 1993

$Si_{3}N_{4}$ 박막의 코팅된 pH-ISFET의 게이트 부위 위에 lipase 효소를 고정화하여 FET(field effect transistor) 형지질(lipid) 센서를 제조하였다. 효소 고정화막 형성 물질로는 수용성 고분자인 polyvinyl alcohol(PVA)에 감광성기인 1-methyl-4-(formylstyryl) pyridinium methosulfate(SbQ)를 결합시킨 PVA-SbQ를 사용하였다. PVA-SbQ 수용액 (SbQ 1mole %, 10 wt%) $200{\mu}L$, bovine serum albumin (BSA) 7.5 mg, lipase 10 mg으로 구성 된 현탁액을 사용한 사진식각(photolithographic) 공정의 최적 조건은 피막의 상온 진공 건조 시간 45분, spin coater의 회전수 $5,000{\sim}6,000$ rpm, UV 노광시간 $20{\sim}30$초, 증류수 현상 시간 $30{\sim}40$초로 나타났다. 이렇게 구성된 지질 센서는 triacetin을 지질 시료로 하였을 때 $10{\sim}100$ mM의 농도 범위에서 직선성의 검정선을 나타내었다.

• Natural Product Sciences
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• 제22권2호
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• pp.93-101
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• 2016

For efficient quality control of the Samryeongbaekchul-san decoction, a powerful and accurate an ultra-performance liquid chromatography (UPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS) method was developed for quantitative analysis of the thirteen constituents: allantoin (1), spinosin (2), liquiritin (3), ginsenoside Rg1 (4), liquiritigenin (5), platycodin D2 (6), platycodin D (7), ginsenoside Rb1 (8), glycyrrhizin (9), 6-gingerol (10), atractylenolide III (11), atractylenolide II (12), and atractylenolide I (13). Separation of the compounds 1 - 13 was performed on a UPLC BEH $C_{18}$ column ($2.1{\times}100mm$, $1.7{\mu}m$) at a column temperature of $40^{\circ}C$ with a gradient solvent system of 0.1% (v/v) formic acid aqueous-acetonitrile. The flow rate and injection volume were 0.3 mL/min and $2.0{\mu}L$. Calibration curves of all compounds were showed good linearity with values of the correlation coefficient ${\geq}0.9920$ within the test ranges. The values of limits of detection and quantification for all analytes were 0.04 - 4.53 ng/mL and 0.13 - 13.60 ng/mL. The result of an experiment, compounds 2, 6, 12, and 13 were not detected while compounds 1, 3 - 5, and 7 - 11 were detected with 1,570.42, 5,239.85, 299.35, 318.88, 562.27, 340.87, 12,253.69, 73.80, and $115.01{\mu}g/g$, respectively.

• 생약학회지
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• 제43권1호
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• pp.10-15
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• 2012

An accurate and sensitive analysis method was established for simultaneous determination of three bioactive compounds (glycyrrhizin, 6-gingerol and ginsenoside Rg3) in the Ejung Tang with high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-Mass spectrometry (MS). The optimizing chromatographic separations a were acquired by an $C_{18}$ column ($5{\mu}m$, $4.6I.D{\times}250mm$, SHISHEDO) using gradient elution with water comprising 0.1% TFA(trifluoroacetic acid) and acetonitrile at a performing temperature of $35^{\circ}C$. Flow rate was 1.0 ml/min. A detection UV wavelength set at 205 nm and 250 nm. The three compounds were identified by electrospray ionization mass spectrometry. All calibration curves indicated great linear regression within test ranges ($R^2>0.9997$). The established method provided acceptable precision and accuracy. The relative standard deviations (RSDs) of intra-day and inter-day were less than 2.00% and 3.00%, respectively. The recoveries were found to range from 94.49 to 101.10% for the three compounds analyzed. These results showed that this method was effective and reliable for quality control of Eiung-Tang.

• Natural Product Sciences
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• 제16권2호
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• pp.116-122
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• 2010

A high-performance liquid chromatography (HPLC) with diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of five representative metabolites of the iridoid and phenolic classes from Rehmannia glutinosa. The optimal chromatographic conditions were obtained on an ODS column (5 mm, $4.6{\times}250\;mm$) with the column temperature at $25^{\circ}C$. The mobile phase was composed of water and acetonitrile using a gradient elution with the flow rate 0.3 mL/min. Detection wavelength was set at 205 nm. All calibration curves showed good linear regression ($r^2$ > 0.997) within test ranges. Limits of detection (LOD) and quantitation (LOQ) values were lower than 0.123 and $0.373\;{\mu}g/mL$, respectively. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.09 - 0.76% and 0.16 - 1.41%, respectively, and the overall recoveries of 99.03 - 102.67% for all of the compounds analyzed. In addition, effectiveness of diverse extraction methods was compared to each other for the development of standard analytic method. The verified method was successfully applied to the quantitative determination of five representative metabolites in twenty-one commercial Rehmannia glutinosa samples from different markets in Korea and China. The analytical results showed that the contents of the five analytes vary significantly with sources.

• 한국물환경학회지
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• 제23권2호
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• pp.228-235
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• 2007

For efficient turbid water withdrawal in the Imha reservoir, a selective withdrawal facility was recently installed and operated during summer season of year 2006. In this research, CE-QUAL-W2 model was utilized to assess the efficiency of the selective withdrawal facility, in comparison with the original surface withdrawal, on turbid water management. Model calibration was carried out using data observed at four automatic monitoring stations in the reservoir. It was found that the model appropriately simulated, with the RMSE less than 5.2 NTU, the observed vertical and horizontal distributions of water temperature and turbidity as well as the location of maximum turbid water at each monitoring station. The analysis results showed that selective withdrawal is more effective in removing high turbid water than surface withdrawal as selective withdrawal contributed to reducing $35Mm^3$ of high turbidity water (> 100 NTU) in the reservoir by increasing outflows of high turbid water. Therefore, effective management of turbid water in the reservoir can be achieved by changing locations of intake depending on turbid water distribution conditions. The results of this study will provide some basic information for establishing better operation strategies to cope with turbid water problems.

• 식품보건융합연구
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• 제6권2호
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• pp.17-21
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• 2020

The response of honeybee (Apis mellifera L.) pollination on canola yield with reference to most suitable number of bee hive need per unit area of crops in order to meet optimum pollination needs and better economic yields by comparing number of hives and yield components an experiment was conducted at Beekeeping and Hill Fruit Pests Research, Station Rawalpindi during 2017-18 in complete randomized block design with two sets of four treatments for comparison: 1 hive acre^-1, 2 hives acre^-1, 3 hives acre^-1 and 0 hive acre^-1. The hives were kept inside the experimental area. Parameters were assessed: pollination density, pollinator's diversity, agronomic and economic yield. In case of pollination density, the cumulative mean abundance bee species revealed that at 1200 hours, Apis mellifera was the most abundant and frequent visitor with a mean population of 8.69 bees/plant followed by A. dorsata (0.72), Syrphid fly (0.2) and other pollinators. Minimum bee population was observed during 1400 hours, mainly due to the closure of flowers and partially due to high temperature (>35℃). Pollinator diversity revealed that A. mellifera was the most dominant pollinator of Brassica crop with highest abundance (71%). A. dosata ranked 2^nd (16%) followed by A. florea (6%) respectively.

• 한국농공학회논문집
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• 제61권6호
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• pp.81-92
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• 2019

The objective of this study is to evaluate hydrologic impacts of climate change according to downscaling methods using the Soil and Water Assessment Tool (SWAT) model at watershed scale. We used the APCC Integrated Modeling Solution (AIMS) for assessing various General Circulation Models (GCMs) and downscaling methods. AIMS provides three downscaling methods: 1) BCSA (Bias-Correction & Stochastic Analogue), 2) Simple Quantile Mapping (SQM), 3) SDQDM (Spatial Disaggregation and Quantile Delta Mapping). To assess future hydrologic responses of climate change, we adopted three GCMs: CESM1-BGC for flood, MIROC-ESM for drought, and HadGEM2-AO for Korea Meteorological Administration (KMA) national standard scenario. Combined nine climate change scenarios were assessed by Expert Team on Climate Change Detection and Indices (ETCCDI). SWAT model was established at the Mankyung watershed and the applicability assessment was completed by performing calibration and validation from 2008 to 2017. Historical reproducibility results from BCSA, SQM, SDQDM of three GCMs show different patterns on annual precipitation, maximum temperature, and four selected ETCCDI. BCSA and SQM showed high historical reproducibility compared with the observed data, however SDQDM was underestimated, possibly due to the uncertainty of future climate data. Future hydrologic responses presented greater variability in SQM and relatively less variability in BCSA and SDQDM. This study implies that reasonable selection of GCMs and downscaling methods considering research objective is important and necessary to minimize uncertainty of climate change scenarios.

• 생약학회지
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• 제49권4호
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• pp.328-335
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• 2018

'EGCG(epigallocatechin gallate) rich Green Tea extract(EGTE)' was prepared by a convenient chromatographical manner using water and alcohol which was regarded as the most suitable and appropriate process for food manufacturing. The EGCG content in EGTE was estimated above 97%. Analysis of polyphenol components in green tea, i.e., catechin(C), epigallocatechin(EGC), epicatechin(EC), epigallocatechin gallate(EGCG), epicatechin gallate(ECG) and caffeine was performed by HPLC. The optimized HPLC method exhibited a good linearity of calibration curve, accuracy and precision. The long-term stability evaluation of EGTE was carried out with a powdered formulation and solution formulation by estimating the color change and measuring the EGCG content by HPLC analysis for one year. The EGCG content of the powdered EGTE stored in a transparent bottle at room temperature was retained over 97% at the end of the experimental period. The EGCG content of 0.1% water solution of EGTE stored in a transparent bottle at RT were observed to decrease below 30%, whereas that stored at $2^{\circ}C$ retained over 70%, respectively. These results suggested that a powdered formulation could be recommended for the commercialized nutraceutical product of EGTE rather than a solution formulation.

• 한국광물학회지
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• 제31권4호
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• pp.277-285
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• 2018

상온에서 남동석($Cu_3(CO_3)_2(OH)_2$)에 대한 고압 거동 연구를 하였다. 대칭형 다이아몬드 앤빌기기를 이용하여 21.52 GPa까지 압력을 증가시키면서 각분산 X-선 회절법과 방사광을 이용하여 고압회절 데이터를 얻었으며, 시료에 가해준 압력은 루비 형광파의 파장 변화를 측정하여 결정하였다. 본 실험에서 시행한 압력의 범위 내에서 상변이는 관찰되지 않았으며, 정압상태에서 체적탄성률($K_0$)은 ${K_0}^{\prime}$이 4일 때, 54.4 GPa로 계산되었다. 상온상태에서 얻은 남동석의 체적탄성률에 대한 신뢰도를 정규화압력 및 정규화응력변형 분석을 통해 검증하였다.

• 인간식물환경학회지
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• 제21권6호
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• pp.485-492
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• 2018

This study aimed to develop and validate highly sensitive determination method of a prostaglandin ($PGE_1$, OP 1206) in human plasma by LC-MS/MS using column switching. Plasma stored at $-30^{\circ}C$ and treated with methanol effectively inhibited interferences synthesized post-sampling. Samples were added with internal standard and were separated by reversed-phase HPLC with a cycle time of 30min. The method was selective for OP 1206 and the regression models, based on internal standard, were linear across the concentration range 0.5-50 pg/mL with the limit of quantification of 0.5 pg/mL (limit of quantitation, LOQ) for OP 1206. The calibration curve of OP 1206 standards spiked in five individual plasma samples was linear ($r^2=0.9999$). Accuracy and precision at the concentrations of 0.5, 1.5, 5.0 and 40 pg/mL, and at the lower LOQ of 0.5 pg/mL were excellent at 20%. OP120 < 6 was stable in plasma samples for at least 24 hours at room temperature, 24 hours frozen at $-70^{\circ}C$, 24 hours in an auto sampler at $6^{\circ}C$, and for two freeze/unfreezing cycles. The validated determination method successfully quantified the concentrations of OP 1206 in plasma samples from simulated administrating a single $5{\mu}g$ OP 1206 formulation. Thus, this novel LC-MS/MS technique for drug separation, detection and quantitation is expected to become the standard highly-sensitive detection method in bioanalysis and to be applied to many low dose pharmaceutical products.