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Simultaneous Determination of Three Compounds in Ejung Tang by HPLC-DAD and LC-ESI-MS  

Lee, Bo-Hyoung (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
Ma, Jin-Yeul (TKM Converging Research Division, Korea Institute of Oriental Medicine)
Weon, Jin-Bae (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
Yang, Hye-Jin (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
Yun, Bo-Ra (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
Ma, Choong-Je (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Pharmacognosy / v.43, no.1, 2012 , pp. 10-15 More about this Journal
Abstract
An accurate and sensitive analysis method was established for simultaneous determination of three bioactive compounds (glycyrrhizin, 6-gingerol and ginsenoside Rg3) in the Ejung Tang with high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-Mass spectrometry (MS). The optimizing chromatographic separations a were acquired by an $C_{18}$ column ($5{\mu}m$, $4.6I.D{\times}250mm$, SHISHEDO) using gradient elution with water comprising 0.1% TFA(trifluoroacetic acid) and acetonitrile at a performing temperature of $35^{\circ}C$. Flow rate was 1.0 ml/min. A detection UV wavelength set at 205 nm and 250 nm. The three compounds were identified by electrospray ionization mass spectrometry. All calibration curves indicated great linear regression within test ranges ($R^2>0.9997$). The established method provided acceptable precision and accuracy. The relative standard deviations (RSDs) of intra-day and inter-day were less than 2.00% and 3.00%, respectively. The recoveries were found to range from 94.49 to 101.10% for the three compounds analyzed. These results showed that this method was effective and reliable for quality control of Eiung-Tang.
Keywords
Ejung Tang; Simultaneous determination; HPLC-DAD; LC-ESI-MS; Validation;
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