• Journal of the Mineralogical Society of Korea
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• v.19 no.4 s.50
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• pp.221-229
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• 2006

Mineralogical and chemical examinations were performed on interstratified illite-smectite (I-S) minerals that occur in the mudstones from a petroleum exploration well in the Tertiary marine basin, Japan. X-ray diffraction analysis shows that component layers of illite in the interstratified I-S increase with increasing burial depth while those of smectie decrease. In addition, the randomly (R=0) interstratified illite-smectite is changed into Rp1 ordered I-S at a depth of about 4,000 m, which corresponds to the result of organic analysis and indicates a burial temperature of about $100^{\circ}C$. However, the present geothermal gradient shows that the conversion of the random I-S to R=0 ordered I-S is likely to occur at 3,000 m. This discrepancy may be interpreted by the reverse fault at 2,500 m which resulted in a deeper burial of sediments up to 1,000 m. Chemical analysis also shows the compositional variation in I-S with increasing depth: a decrease in Si and an increases in Al and K, indicating that the substitution of Al for Si in tetrahedral sheets is compensated by the addition of K to interlayers. K may be derived from K-feldspar and micas, which is present in the mudstones.

• Korean Journal of Materials Research
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• v.8 no.9
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• pp.831-836
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• 1998

The B1914 Ni-base superalloy was manufactured according to crystal structures of poly-, directionally solidified- and single crystals. We observe deformation as type of different crystal structure from room to high temperature. Specimens are controled by cooling rate and thermal gradient and then heat treatment in vacuum and then cooling with Ar gas. Different crystal structure has different stress-strain characteristic. At $600^{\circ}C$, yield strength and ultimate strength is increased single-, directionally solidified- and poly crystals in order.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.62-68
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• 2016

The feeder cable assembly is an automotive part used for telecommunication. If it malfunctions, the control and safety of the automobile can be put at risk. ALT (Accelerated Life Testing) is a testing process for products in which they are subjected to conditions (stress, strain, temperatures, etc.) in excess of their normal service parameters in an attempt to uncover faults and potential modes of failure in a short amount of time. Failure is caused by defects in the design, process, quality, or application of the part, and these defects are the underlying causes of failure or which initiate a process leading to failure. Thermal shock occurs when a thermal gradient causes different parts of an object to expand by different amounts. Thermal shock testing is performed to determine the ability of parts and components to withstand sudden changes in temperature. In this research, the main causes of failure of the feeder cable assembly were snapping, shorting and electro-pressure resistance failure. Using the Coffin-Manson model for ALT, the normal conditions were from Tmax = $80^{\circ}C$ to Tmin = $-40^{\circ}C$, the accelerated testing conditions were from Tmax = $120^{\circ}C$ to Tmin = $-60^{\circ}C$, the AF (Acceleration Factor) was 2.25 and the testing time was reduced from 1,000 cycles to 444 cycles. Using the Bxlife test, the number of samples was 5, the required life was B0.04%.10years, in the acceleration condition, 747 cycles were obtained. After the thermal shock test under different conditions, the feeder cable assembly was examined by a network analyzer and compared with the Weibull distribution modulus parameter. The results obtained showed good results in acceleration life test mode. For the same reliability rate, the testing time was decreased by a quarter using ALT.

• The Journal of Engineering Geology
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• v.24 no.4
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• pp.487-499
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• 2014

While the vertical open type of heat exchanger is more effective in areas of abundant groundwater, and is becoming more widely used, the heat exchanger most commonly used in geothermal heating and cooling systems in Korea is the vertical closed loop type. In this study, we performed numerical simulations of the optimal utilization of geothermal energy based on the hydrogeological and thermal properties to evaluate the efficiency of the vertical open type in areas of abundant groundwater supply. The first simulation indicated that the vertical open type using groundwater directly is more efficient than the vertical closed loop type in areas of abundant groundwater. Furthermore, a doublet system with separated injection and extraction wells was more efficient because the temperature difference (${\Delta}$) between the injection and extraction water generated by heat exchange with the ground is large. In the second simulation, we performed additional numerical simulations of the optimal utilization of geothermal energy that incorporated heat transfer, distance, flow rate, and groundwater hydraulic gradient targeting a single well, SCW (standing column well), and doublet. We present a flow diagram that can be used to select the optimal type of heat exchanger based on these simulation results. The results of this study indicate that it is necessary to examine the adequacy of the geothermal energy utilization system based on the hydrogeological and thermal properties of the area concerned, and also on a review of the COP (coefficient of performance) of the geothermal heating and cooling system.

• Korean Journal of Veterinary Research
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• v.24 no.1
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• pp.40-49
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• 1984

To investigate the factors influencing the artifical transformation in Escherichia coli, E. coli C600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCl-Etbr equilibrium density gradient centrifugation from E.coli HB 101. The influencing factors in the transformation such as concentration of calcium chloride, time of ice incubation, temperature and time of heat shock, time of gene expression, effects of plasmid DNA concentration and adding time were examined in these experiments. The results obtained were as follows; 1. The highest transformation frequency was observed in the treatments of 100 mM $CaCl_2$ before heat shock and the treatment of $CaCl_2$ was essential step in the process of E. coli transformation. 2. The highest transformation frequency was observed in the treatment of heat shock at $42^{\circ}C$ for 4 min. or $37^{\circ}C$ for 6 min., but the prolonged heat shock resulted a decreased transformation frequency. 3. Treatments of ice incubation at $0^{\circ}C$ for 45 min. before heat stocks or at $0^{\circ}C$ for 30min. after heat shock resulted an increased transformation frequency. 4. There was a linear relationship between DNA concentration and transformation frequency at the concentration of $8{\times}10^3$ recipient cells. The highest transformation frequency reached in carte of 7 mcg of donor DNA, but above 1 mcg of DNA concentration, transformation frequency was not remarkably increased. Addition of donor DNA just after the treatment of $CaCl_2$ was the best. 5. The best condition of gene expression at $37^{\circ}C$ were 40min. for TC-resistant gene and 100min. for AP-resistant gene. TC-resistant gene was higher in the transformation frequency and faster in the gene expression time than AP-resistant gene. In these results, the best conditions for the transformation of E. coli C 600 with pBR322 DNA were: treatment with 100mM $CaCl_2$, ice incubation at $0^{\circ}C$ for 45 min, heat shock at $42^{\circ}C$ for 4 min., 30 min. of ice incubation and incubation at $37^{\circ}C$ for 100min. for gene expression in that order.

• International Journal of Highway Engineering
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• v.19 no.6
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• pp.67-74
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• 2017

PURPOSES : In this study, a three-dimensional nonlinear finite element analysis (FEA) model for airport concrete pavement was developed using the commercial program ABAQUS. Users can select an analysis method and set the range of input parameters to reflect actual conditions such as environmental loading. METHODS : The geometrical shape of the FEA model was chosen by considering the concrete pavement located in the third-stage construction site of Incheon International Airport. Incompatible eight-node elements were used for the FEA model. Laboratory test results for the concrete specimens fabricated at the construction site were used as material properties of the concrete slab. The material properties of the cement-treated base suggested by the Federal Aviation Administration(FAA) manual were used as those of the lean concrete subbase. In addition, preceding studies and pavement evaluation reports of Incheon International Airport were referred for the material properties of asphalt base and subgrade. The kinetic friction coefficient between the concrete slab and asphalt base acquired from a preceding study was used for the friction coefficient between the layers. A nonlinear temperature gradient according to slab depth was used as an input parameter of environmental loading, and a quasistatic method was used to analyze traffic loading. The average load transfer efficiency obtained from an Heavy falling Weight Deflectomete(HWD) test was converted to a spring constant between adjacent slabs to be used as an input parameter. The reliability of the FEA model developed in this study was verified by comparing its analysis results to those of the FEAFAA model. RESULTS : A series of analyses were performed for environmental loading, traffic loading, and combined loading by using both the model developed in this study and the FEAFAA model under the same conditions. The stresses of the concrete slab obtained by both analysis models were almost the same. An HWD test was simulated and analyzed using the FEA model developed in this study. As a result, the actual deflections at the center, mid-edge, and corner of the slab caused by the HWD loading were similar to those obtained by the analysis. CONCLUSIONS : The FEA model developed in this study was judged to be utilized sufficiently in the prediction of behavior of airport concrete pavement.

• Korean Journal of Animal Reproduction
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• v.17 no.4
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• pp.339-346
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• 1994

An understanding of the structure and function of mammalian spermatozoa requires the iso-lation of these components. In this study, frozen-thawed bovine spermatozoa were treated by physical treatments (vortexing, 26 gauge needle, strained 26 gauge needles and freezing-thawing) or chemical treatments (trypsin, dithiothreitol, sodium dodecylsulfate and $\beta$-mercaptoethanoJ) to yield free heads and tails. The most effective treatment was repeated pumping of sperm suspension through a strained 26 gauge needle conneted to a syringe. Spermatozoa by this treatment were mainly broken at the junction of the head and the tail, resulting in 90-100% yields. Also, sperm head surface did not modify during strained 26 gauge needle treatment when either spermatozoa or sperm heads were incubated in 250${\mu}\textrm{g}$/ml of FITC-UEA 1 for 1 h at room temperature to detect the modification of sperm surface components. Other physical treatments were less efficient for the breakdown of spermatozoa. The effects of chemical treatments on bovine spermatozoa are not noticeable. Dissected sperm heads and tails should be fractional leading to nearly pure components by sucrose gradient centrifugation at 1,000 rpm for 15 min. The result suggest that the established method may be useful for the biochemical study of spermatozoal components, and the understanding of oocyte activation mechanism either by spermatozoal components during fertilization or microinjection of isolated components.

• The Korean Journal of Mycology
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• v.17 no.2
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• pp.91-98
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• 1989

Mitochondria in the L. edodes was separated and purified by stepped sucrose density gradient centrifugation. The activity of mitochondrial ATP synthase has been investigated during various illumination times at each wavelength within the range of 400 nm to 700 nm. The stimulation of above activity increased by two times compared with nonilluminated control group when the illumination was given for 15 seconds at 470 nm wavelength. The optimal pH and temperature of this light-induced mitochondrial ATP synthase were 7.5 and $54^{\circ}C$, respectively. The activity of this enzyme increased by 26%, 25% and 14%, respectively, when there were 1 mmole $Fe^{3+}$, 0.5 mmole $Fe^{2+}$, and 5 mmole ${SO_4}^{2-}$ ion, and was inhibited by 5 mmole $Co^{2+}$, 5 mmole $Mn^{2+}$, 1 mmole $Ca^{2+}$, 0.1 mmole $Na^+$, 5 mmole $CN^-$, and 0.1 mmole ${CO_3}^{2-}$ ion. But $Na^+$ and $K^+$ ion did not affect the activity of enzyme.

• Korean Journal of Medicinal Crop Science
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• v.21 no.6
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• pp.486-492
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• 2013

This study has been conducted to establish the optimal extraction process and HPLC analysis method for the determination of marker compounds as a part of the materials standardization for the development of health functional food materials from Astragali radix. Five extraction conditions including the shaking extraction at room temperature and the reflux extraction at $85^{\circ}C$ with 30%, 50% and 95% ethanol were evaluated. Reflux extraction with 50% ethanol showed the highest extraction yield as $27.27{\pm}2.27%$, while the extraction under reflux with 95% ethanol showed significantly the lowest yield of $10.55{\pm}0.24%$. The quantitative determination methods of calycosin-7-O-${\beta}$-D-glucoside and calycosin as marker compounds of Astragali radix extracts were optimized by HPLC analysis using a Thermo Hypersil column ($4.6{\times}250mm$, $5{\mu}m$) with the gradient elution of water and acetonitrile as the mobile phase at the flow rate of $0.8mLmin^{-1}$ and a detection wavelength of 230nm. The HPLC/UV method was applied successfully to the quantification of two marker compounds in Astragali radix extracts after validation of the method with the linearity, accuracy and precision. The contents of calycosin-7-O-${\beta}$-D-glucoside and calycosin in 50% ethanol extracts by reflux extraction were significantly higher as $1,700.3{\pm}30.4$ and $443.6{\pm}8.4{\mu}g-1$, respectively, comparing with those in other extracts. The results indicate that the reflux extraction with 50% ethanol at $85^{\circ}C$ is optimal for the extraction of Astragali radix, and the established HPLC method are very useful for the evaluation of marker compounds in Astragali radix extracts to develop the health functional material from Astragali radix.

• Microbiology and Biotechnology Letters
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• v.39 no.3
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• pp.301-307
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• 2011

Oil pollution was world-wide prevalent treat to the environment, and the physic-chemical remediation technology of the TPH (total petroleum hydrocarbon) contaminated soil had the weakness that its rate was very slow and not economical. Bioremediation of the contaminated soil is a useful method if the concentrations are moderate and non-biological techniques are not economical. The aim of this research is to investigate the influence of additives on TPH degradation in a diesel contaminated soil environment. Six experimental conditions were conduced; (i) diesel contaminated soil, (ii) diesel contaminated soil treated with microbial additives, (iii) diesel contaminated soil treated with microbial additives and the mixture was titrated to the end point of pH 7 with NaOH, (iv) diesel contaminated soil treated with microbial additives and accelerating agents and (v) diesel contaminated soil treated with microbial additives and accelerating agents, and the mixture was titrated to the end point of pH 7 with NaOH. After 10 days, significant TPH degradation (67%) was observed in the DSP-1 soil sample. The removal of TPH in the soil sample where microbial additives were supplemented was 38% higher than the control soil sample during the first ten days. The microbial additives were effective in both the initial removal rate and relative removal efficiency of TPH compared with the control group. However, various environmental factors, such as pH and temperature, also affected the activities of microbes lived in the additives, so the pH calibration of the oil-contaminated soil would help the initial reduction efficiency in the early periods.